Heavy Metals In Plants Research Articles

MAPK Cascades and Transcriptional Factors: Regulation of Heavy Metal Tolerance in Plants.

In nature, heavy metal (HM) stress is one of the most destructive abiotic stresses for plants. Heavy metals produce toxicity by targeting key molecules and important processes in plant cells. The mitogen-activated protein kinase (MAPK) cascade transfers the signals perceived by cell membrane surface receptors to cells through phosphorylation and dephosphorylation and targets various effector proteins or transcriptional factors so as to result in the stress response. Signal molecules such as plant hormones, reactive oxygen species (ROS), and nitric oxide (NO) can activate the MAPK cascade through differentially expressed genes, the activation of the antioxidant system and synergistic crosstalk between different signal molecules in order to regulate plant responses to HMs. Transcriptional factors, located downstream of MAPK, are key factors in regulating plant responses to heavy metals and improving plant heavy metal tolerance and accumulation. Thus, understanding how HMs activate the expression of the genes related to the MAPK cascade pathway and then phosphorylate those transcriptional factors may allow us to develop a regulation network to increase our knowledge of HMs tolerance and accumulation. This review highlighted MAPK pathway activation and responses under HMs and mainly focused on the specificity of MAPK activation mediated by ROS, NO and plant hormones. Here, we also described the signaling pathways and their interactions under heavy metal stresses. Moreover, the process of MAPK phosphorylation and the response of downstream transcriptional factors exhibited the importance of regulating targets. It was conducive to analyzing the molecular mechanisms underlying heavy metal accumulation and tolerance.

Coal gangue modified bioretention system for runoff pollutants removal and the biological characteristics

In this study, coal gangue (CG) was applied as media in bioretention system to remove runoff pollutant. CG modified bioretention systems show good removal efficiency towards runoff pollutant due to the high adsorption capacity of CG. The removal of total phosphorus (TP), total nitrogen (TN), ammonia (NH4+-N) and chemical oxygen demand (COD) by CG modified bioretention systems was influenced by diverse rainfall conditions including rainfall concentration, recurrence period and drying period, and their removal rate ranged 94–99%, 30–70%, 83–97% and 33–86%, respectively. The effluent concentration of Zn, Pb and Cu was as low as 3.14–10.99 μg/L, 0.66–2.56 μg/L and 0.60–3.15 μg/L, respectively. In addition, CG could promote the plant heavy metal uptake and thus decrease their accumulation in soil to a certain extent. Meanwhile, Malondialdehyde (MDA) content and peroxidases (POD) activities of plants in CG modified bioretention were lower than that in tradition bioretention, indicating that CG could help plants recovery and lessened the oxidative stress for the negative impact of high heavy metals accumulation. CG-based media alleviated the inhibitory effect of rainwater runoff pollutant accumulation (especially heavy metals) on microbial diversity and the enhancement of the dominant bacteria (such as Proteobacteria and Bacteroidota) could conduce the nutrients removal in the bioretention systems. In overall, this study demonstrated that the CG modified bioretention systems show an excellent removal performance combine with biological effects.

Can Potato Crop on Sandy Soil Be Safely Irrigated with Heavy Metal Polluted Water?

Heavy metal (HM) accumulation in soil and plants can occur when water contaminated with HMs is used as a source of irrigation (El-Salam Canal, Egypt). In this study, the effect of watering potato crop in sandy soil from a polluted water source under flood irrigation (FI), sprinkler irrigation (SI), and surface drip irrigation (DI) on the transport of the HMs copper (Cu), manganese (Mn), lead (Pb), and zinc (Zn) in the root zone was experimentally investigated. HM concentrations in potato plant parts was also determined. The field experiments were conducted in a completely randomized block with three replicates for each irrigation method by using nine field lysimeters. Soil and plant samples were collected at the end of the growing season to determine their HM content. The results showed that regardless of irrigation method, irrigation with HM contaminated water raised HM concentrations in both soil and potato plants. DI produced the highest concentrations of most HMs (Cu, Mn, and Pb) in the upper soil layer (0–40 cm) and highest Cu, Pb, and Zn concentrations in plant tubers as well. Maximum Zn concentration in the upper soil layer and maximum Mn concentration in plant tubers occurred under SI. The maximum concentrations of Cu, Mn, Pb, and Zn in both the upper soil layer and plant tubers were 12.0, 140.0, 11.6 and 67.9 mg/kg and 6.3, 9.4, 2.3 and 23.9 mg/kg, respectively. However, FI produced the highest concentrations in the deep soil layer (40–60 cm) and the least concentration of HMs in plant tubers. These concentrations were 18.8, 203.8, 13.3 and 70 mg/kg and 4.0, 6.0, 0.6 and 17.1 mg/kg in soil and plant tubers for Cu, Mn, Pb, and Zn, respectively. The maximum concentrations of HMs in soil and potato plants were lower than the maximum permissible limits. Therefore, El-Salam Canal water appears not to be harmful in the short term. However, as shown in the results, HM accumulation depends on irrigation technique; thus, more studies are needed to determine harmful effects in the long term.

Endophyte colonization enhanced cadmium phytoremediation by improving endosphere and rhizosphere microecology characteristics

This study investigated the phytoremediation efficiency of Cd-contaminated soils by hyperaccumulator P. acinosa and its endophyte B. cereus, and evaluated the variation of rhizosphere/endosphere microecology characteristics. The result showed that endophyte PE31, which could successfully colonize on P. acinosa root, increased plant Cd uptake by 42.90% and 28.85% in low and high Cd contaminated soils by promotion of plant biomass and Cd concentration in plant tissues. The improved phytoremediation may attribute to the endophyte inoculation, which significantly improved the bioavailable heavy metal (HM) percentage, nutrient cycling related enzyme activities and nutrient contents including available potassium, phosphorus and organic matter. Additionally, the relative abundance beneficial bacteria Bacillus (significantly increased by 81.23% and 34.03% in the endosphere, and by 4.86% and 8.54% in rhizosphere in low and high Cd contaminated soils) and Lysobacter, showed positive and close correlation with plant growth and HM accumulation. These results indicated that endophyte inoculation could reshape rhizosphere and endosphere microecology characteristics, which enhanced the potential for phytoremediation of Cd contaminated soils.

Measurement of heavy metals in soil, plants and water samples based on MWCNTs modified with Bis(triethoxysilylpropyl)tetrasulfide by flame atomic absorption spectrophotometry

Heavy metals (HMs) are considered as the major environmental pollutants that accumulated in soil and plant. Consumption of such contaminated plants by humans and animals would ultimately harm the health of communities. This study aims to evaluate the amount of copper(Co), cadmium(Cd), and lead(Pb) in soil and cultivated plants that are irrigated by the city of Zabol’s wastewater. Also, the heavy metals determined in 20 mL of Zabol’s water based on Bis(triethoxysilylpropyl)tetrasulfide (S4[C3H6Si(OEt)3]2, TEOSiP-TS) modified on MWCNTs as an adsorbent by the uniform dispersive -micro-solid phase extraction (UD-µ-SPE) at optimized pH. In this study, 52 samples including wheat, corn grain, and wild spinach, as well as agricultural soil were selected randomly from three village stations. The concentrations of heavy metals in plants, soils, and water samples were measured using a flame atomic absorption spectrometer (F-AAS). By optimizing parameters, the linear range (LR) and the detection limit (LOD) of Cu, Cd, and Pb were obtained 1.5-1000 μg L-1, 1-200 μg L-1, 5-1500 μg L-1 and 0.5 μg L-1, 0.25 μg L-1, 1.5 μg L-1, respectively in water samples (RSD%<2). This study indicates that irrigation of agricultural fields using wastewater causes the accumulation of heavy metals in soil and plants.

Accumulation of Heavy Metals in Plants of the Genus Achillea L. under Arid Conditions of the Plain Zone of Dagestan

Accumulation of Heavy Metals in Plants of the Genus Achillea L. under Arid Conditions of the Plain Zone of Dagestan

Assessment of Heavy Metals in Fruits and Vegetables Collected from Bareilly Local Market, Uttar Pradesh State, India

Abstract: Heavy metals are among the contaminating agents of our food supply, and the main source of metals or the media through which living beings get exposed to various toxic metals is food and water. The concentration of heavy metals in plants is essential for good health and growth of human and animals but should be within permissible limits. Various natural and anthropogenic factors are responsible for excess metal concentration in crops. Dietary heavy metals such as cadmium, lead, arsenic, mercury can be harmful to biota as their excessive accumulation in human body can lead to systematic health problems. Fruits and vegetables are main component in diet after cereals. Hence the present study aimed to determine Aluminium (Al), Arsenic (As), Cadmium (Cd), Lead (Pb) and Mercury (Hg) content in mostly consumed vegetables (onion, potato, taro root, spinach, taro root leaves, beans, jackfruit and pumpkin) and fruits (blackberry, mango and pears) during summers in Bareilly City, Uttar Pradesh. These food samples were collected from local vendors and shops from four different market sites of the city. The samples were analysed using atomic absorption spectrophotometer and the obtained results were compared with safe limits set by different national and international agencies. Based on the data we conclude that concentrations (mg/kg dry weight) of As, Pb and Hg for all samples and Al and Cd for most of the food stuffs were found exceeding the allowable limits causing potential health risk to consumers of present study area. Elevated levels of these metals in food stuffs might be due to various factors such as usage of industrial and disposal waste water for irrigation, municipal and urban runoff, fertilizers, pesticides, that contaminates the soil and hence crops absorb those metals. Some cultivation areas get exposed to atmospheric pollution found near highways, in form of metal containing aerosols. Other factors can be exposure to pollution during transportation and marketing. The prolonged consumption of unsafe concentration of metals is an alarming public health concerns, considering that metals can accumulate in organism and show negative effects by causing various disruptions of numerous biochemical processes and therefore leading to acute and chronic health outcomes. Such foodstuffs should be avoided in the diet and a regular monitoring is required to avoid the health risk among humans. Keywords: heavy metal, contamination, concentration, food sample, human, toxicity

Effects of fulvic acid and humic acid from different sources on Hg methylation in soil and accumulation in rice

Humus is often used as an organic modifier to reduce the bioaccumulation of heavy metals in plants, but the effects of different humus components from different sources on the fate of mercury (Hg) in paddy fields are still unclear. Here, fulvic acid (FA) and humic acid (HA) extracted from composted straw (CS), composted cow dung (CCD), peat soil (PM) and lignite coal (LC) were used to understand their effects on the methylation and bioaccumulation of Hg in paddy soil by pot experiments. Amendments of both FA and HA largely increased the abundance of Hg-methylating microbes and low-molecular-weight organic matters (e.g, cysteine) in paddy soil. They were also found to change the aromaticity, molecular size and Chromophoric DOM concentration of DOM, and resulted in heterogeneous effects on migration and transformation of Hg. All the FA-amended treatments increased the mobility and methylation of Hg in soil and its absorption in roots. Nevertheless, FA from different sources have heterogeneous effects on transport of Hg between rice tissues. FA-CCD and FA-PM promoted the translocation of MeHg from roots to rice grains by 32.95% and 41.12%, while FA-CS and FA-LC significantly inhibited the translocation of inorganic Hg (IHg) by 52.65% and 66.06% and of MeHg by 46.65% and 36.23%, respectively. In contrast, all HA-amended treatments reduced the mobility of soil Hg, but promoted Hg methylation in soil. Among which, HA-CCD and HA-PM promoted the translocation of MeHg in rice tissues by 88.95% and 64.10%, while its accumulation in rice grains by 28.43% and 28.69%, respectively. In general, the application of some FA and HA as organic modifiers to reduce Hg bioaccumulation in rice is not feasible.

Increase Potential of Environmental Stresses and Climatic Risk of Heavy Metals in Plants and Control through Nanotechnology Advances

Different heavy salts accumulated in the soil causing the soil salinity and damage the biological processing occurring in plants such as photosynthesis and light reactions. Climate changes also promote the accumulation of heavy metals by promoting the chemicals substances in the leaves and roots cells that causing the chlorosis. Coal combustion release the large amount of mercury metals that acting as toxic for the plants. There is need to design the biological and mechanical processing the removal of salts and toxic wastes from surfaces in such a way that no eruptions of plant materials occur at the surfaces. Nanotechnology employed the nanoparticles to capture the toxic metals through biological and chemical process that is more reliable and less expensive. A large variety of nanoparticles in the form of nanobased graphene oxides, graphite oxides and CNT, mesoporous silica materials have been used for controlling the pollution caused by heavy metals. These nanoparticles are compatible for the plant growth by suppressing the crop diseases by acting directly on phytopathogens. Therefore, it is reliable for controlling the risk of environmental pollution caused by heavy metals.

Increase of P and Cd bioavailability in the rhizosphere by endophytes promoted phytoremediation efficiency of Phytolacca acinosa

The information about the spatial distribution of bioavailable phosphorus (P) and heavy metal (HM) in the rhizosphere could aid in the precise phytoremediation regulation. In this study, a rhizobox system was adapted to study soil-root interactions and used to access the endophyte inoculation variation on bioavailable P and cadmium (Cd) spatial distribution during phytoremediation of Cd contaminated soils. Results showed that endophyte PE31 Bacillus cereus inoculation enhanced Cd uptake of P. acinosa by 52.70% and 46.73% in low and high Cd contaminated soils, increasing the phytoremediation hotspot area from 45.78% and 15.29% to 60.97% and 21.80%, respectively. Available P and Cd significantly diminished because root activities depleted large amounts of bioavailable P and Cd concentrations. However, PE31 increased bioavailable P and Cd concentration in the rhizosphere soil. The bioavailable P enhancement in the rhizosphere was positively correlated to plant growth and Cd accumulation. Overall, endophyte inoculation compensated the diminution of bioavailable P and Cd in the rhizosphere to improve plant biomass and HM absorption, and thus promote phytoremediation efficiency. This study helped to better understand bioavailable P and Cd spatial distribution under endophyte inoculation, which could provide effective management strategies for the precise regulation of phytoremediation.

Characteristics of Plant Diversity and Heavy Metal Enrichment and Migration Under Different Ecological Restoration Modes in Abandoned Mining Areas

In order to select plant materials suitable for the ecological restoration of abandoned mining land, ecological restoration experiments were set up in landfills. The effects of different ecological restoration measures on plant diversity and heavy metal enrichment and migration characteristics were studied. The results showed that under different ecological restoration measures, a total of eight families and 10 species of surviving plants appeared, most of which were herbs. The restoration effect of the arbor shrub and grass plots was the best; the plant coverage reached 100%, and the survival rate was over 69%. The diversity index of the plant community was as follows:arbor shrub grass > shrub grass > joe grass > grass. The dominant plants under different ecological restoration measures were Pinus elliottii, Ligustrum lucidum, Boehmeria nivea, Lagerstroemia indica, and Plantago asiatica. The contents of most heavy metals in plants were higher than the normal values. Among them, the enrichment and transfer coefficients of the leaf stems of P. elliottii and L. indica were close to or greater than 1, showing a strong ability of enrichment and transfer to Cd; these two plants belong to Cd-enrichment type plants. The roots of L. lucidum Ait and P. asiatica, which are root-hoarding plants, had a high retention rate of heavy metals. Boehmeria nivea was a heavy metal-avoidance plant with a low content of heavy metals in each part and had the ability to fix and repair potential soil heavy metal pollution. Considering the diversity of plants and the ability of plant enrichment and migration, the combination of trees, shrubs, and grasses was the best restoration mode. Pinus elliottii, L. indica, and P. asiatica can be the target plants for ecological restoration in the abandoned land of mining areas.

Stabilization of heavy metals in biochar derived from plants in antimony mining area and its environmental implications

Heavy metals pollution in mining soils seriously threatens the ecological environment and human health worldwide. Phytoremediation is considered to be an ideal method to reduce the toxicity, mobility, and bioavailability of heavy metals in the soils. However, the disposal of plant-enriched heavy metals has become a thorny problem. To estimate the effect of pyrolysis on the stabilization of heavy metals in post-phytoremediation plant residues, different biochars were prepared from Conyza canadensis (CC), Gahnia tristis (GT), and Betula luminifera (BL) at different pyrolysis temperatures (300, 450, and 600 °C). Results indicated that pyrolysis was effective in the stabilization of heavy metals (Cr, Ni, As, Sb, Hg, and Pb) in plants and significantly (P < 0.05) decreased the bioavailability of most heavy metals. Among them, GT600 prepared by pyrolysis of GT at 600 °C has the best stabilization effect on Sb, which increases the residual fraction by 7.32 times, up to 82.05%. The results of environmental risk assessment show that pyrolysis of biomass at high temperature (600 °C) can effectively mitigate the environmental impact of As, Sb, and Hg. Additionally, the reutilization potential of biochar produced by post-phytoremediation plant residues as adsorbents was investigated. The results of adsorption experiments revealed that all biochars have an excellent performance to adsorb Pb(II), and the maximum adsorption capacity is 139.16 mg g−1 for CC450. The adsorption mechanism could be attributed to complexation, electrostatic attraction, and cation exchange. This study demonstrates that pyrolysis is an effective and environment-friendly alternative method to stabilize heavy metals in plants, and their pyrolysis products can be reused for heavy metal adsorption.

Fluorescent Molecular Sensor for the Detection of Cadmium in Basil Roots

In this work, a methodological route to find a fluorescent radiometric OFF–ON type configuration of rhodamine B with diethylenetriamine dissolved in ethanol for the detection of Cd2+ on the surface of basil roots is presented. The proposed fluorescent configuration has a very fast response time and is highly selective and sensitive in a linear detection range between 10 and 1000 μg/L. Furthermore, the obtained quantum efficiency of 0.63 projects this configuration as a strategic option to be applied in the development of fluorescent molecular sensors for the in situ detection of heavy metals like cadmium in plant species. This approach contributes to addressing the critical need to monitor, detect, and quantify heavy metals in plants meant for human consumption at the local, national, and global levels.

Study the effect of operating of the North and South Al Diwaniyah power plants on the city of Al Diwaniyah

This study was conducted to find out the impact of two diesel plants (southern and northern) of the city of Al Diwaniyah by studying the concentrations of some heavy elements (Cu, Pb, Zn, Mn and Fe) in the soil and plants around the two stations as a result of operating with heavy fuel and the absence of techniques for filtering exhaust waste. The results showed that heavy metals in plants are higher than their concentration in the soil. Moreover, the highest percentage of contamination with heavy elements for each of Fe, Zn, Mn, Cu and Pb in the soil is 293.1, 133.8, 365, 55, 46 and 0.093 mcg/gm, respectively against, 440, 201.8, 446, 130,98 and 35.12 mcg/gm in the plant. These percentages are higher than the recommended values, according to the World Health Organization (WHO). The results also showed that the pollution level from heavy metals in soil and plant samples was higher in the areas south of the two stations. The lowest values were located to the North of stations because the wind blew from North to south, carrying the pollutants most of the year. This has resulted from the incomplete combustion of heavy fuel in the two plants to the populated city center, located to the south of the North Al Diwaniyah station.

Nationwide Review of Heavy Metals in Municipal Sludge Wastewater Treatment Plants in China: Sources, Composition, Accumulation and Risk Assessment

Nationwide Review of Heavy Metals in Municipal Sludge Wastewater Treatment Plants in China: Sources, Composition, Accumulation and Risk Assessment

Effect of iron slag, zeolite, and Piriformospora indica fungus on mazut biodegradation in a heavy metal-polluted soil that was amended with cow manure under canola cultivation

Aims: Petroleum hydrocarbon pollution in the soil is one of the important problems in environmental studies. Thus, this research was conducted to evaluate the effect of iron slag, zeolite, and Piriformospora indica fungus on mazut biodegradation in a heavy metal-polluted soil that was amended with cow manure under canola cultivation. Materials and Methods: Treatments included application of zeolite (0% and 2% [W/W]) and iron slag enriched cow manure (0, 15, and 30 t/ha) in a mazut-polluted soil (0%, 4%, and 8% [W/W]) under canola cultivation in the presence of P. indica. After 70 days, plants were harvested and the mazut biodegradation in the soil was measured. In addition, the soil and plant Pb and Cd concentration was measured using atomic absorption spectroscopy. Findings: Soil application of zeolite (2% [W/W]) in the mazut-polluted soil (4% [W/W]) significantly increased the mazut biodegradation in the soil by 13.1%. In addition, plant inoculation with P. indica significantly increased the mazut biodegradation in the soil and decreased the plant Pb and Cd concentration. Soil application of cow manure at the rate of 15 and 30 t/ha has also increased the mazut biodegradation in the soil by 14.1% and 17.4%, respectively. Conclusion: Using iron slag enriched cow manure and zeolite had additive effects of increasing the mazut biodegradation in the soil and decreasing the soil and plant heavy metal concentration. However, these changes depend on the plant physiology and soil physic-chemical properties that should be considered in different studies.

Oshipp17 is Involved in Plant Heavy Metal Toxic Response Via Affecting Cytokinin Homeostasis

Oshipp17 is Involved in Plant Heavy Metal Toxic Response Via Affecting Cytokinin Homeostasis

Environmental safety monitoring of heavy metals accumulation in some species of Sambucus L.

The aim in this work was to monitor heavy metals in plants of elderberry species. The objective of the research was to identify the actual concentrations of heavy metals in the fruits and leaves of the studied elderberry species. The need to determine deviations in the content of heavy metals in various parts of plants arose because the current ecological situation in Belgorod’s area requires special attention. The presence and concentration of heavy metals in different elderberry species and different parts of plants vary greatly and have the following features. Increased magnesium content was observed in almost all elderberry species, especially in red-fruited (Sambucus racemosa ‘Aurea’, S. sibirica Nakai, S. racemosa L., S. racemosa ‘Plumosa’, S. coreana Nakai), And its concentration much higher in fruits than in leaves. The highest deviation of the magnesium content from the norm was noted in the fruits and leaves of S. sibirica (2 and 1.5 times, respectively). Black elderberry species (S. nigra L., S. canadensis L., S. canadensis ‘Plumosa’) have a lower magnesium concentration in fruits and leaves. On the other hand, the concentration of copper in fruits and leaves in elderberry species is many times higher than the norm.

Genome-wide identification and genetic characterization of the CaMYB family and its response to five types of heavy metal stress in hot pepper (Capsicum annuum cv. CM334)

MYB proteins play a crucial role in plant growth and development and stress responses. In this study, 160 members of the MYB gene family from the pepper genome database were used to analyze gene structures, chromosome localization, collinearity, genetic affinity and expression in response to heavy metals. The results identified R2R3-MYB members and further phylogenetically classified them into 35 subgroups based on highly conserved gene structures and motifs. Collinearity analysis showed that segmental duplication events played a crucial role in the functional expansion of the CaMYB gene family by intraspecific collinearity, and at least 12 pairs of CaMYB genes existed between species prior to the differentiation between monocots and dicots. Moreover, the upstream CaMYB genes were mainly localized to the phytohormone elements ABRE and transcription factor elements MYB and MYC. Further analysis revealed that MYB transcription factors were closely associated with a variety of abiotic stress-related proteins (e.g., MAC-complex and SKIP). Under the stress of five metal ions, Cd2+, Cu2+, Pb2+, Zn2+, and Fe3+, the expression levels of some CaMYB family genes were upregulated. Of these genes, pairing homologous 1 (PH-1), PH-13, and PH-15 in the roots of Capsicum annuum were upregulated to the greatest extent, indicating that these three MYB family members are particularly sensitive to these five metals. This study provides a theoretical reference for the analysis of the molecular regulatory mechanism of MYB family genes in mediating the response to heavy metals in plants. This study reveals the mode of interaction between MYB and a variety of abiotic stress proteins and clarifies the biological functions of CaMYB family members in the regulation of heavy metal stress.

Heavy metal ATPase genes (HMAs) expression induced by endophytic bacteria, “AI001, and AI002” mediate cadmium translocation and phytoremediation

Contamination of heavy metals is a serious threat, which causes threats to the environment. Our study aimed to determine the role of endophytic bacteria in Cd phytoremediation and heavy metal ATPase gene expression. Cadmium (Cd) resistant endophytic bacteria were isolated from Solanum nigrum on LB agar plates, contaminated with 0–30 mg/L Cd. The phosphate solubilization and indole-3-acetic acid (IAA) production of endophytes were estimated by growing them on Pikovskaya agar medium and GC-MS analysis, respectively. An experiment in a pot was performed to evaluate the effects of bacteria on rice plants contaminated with 5–25 mg/L of Cd. Expression of Cd response genes was quantified through qRT–PCR and Cd translocation from one part to another part of the plant was measured through the ICP. BLAST alignment of 16 S-rDNA gene sequences confirmed the bacterial isolates as Serratia sp. AI001 and Klebsiella sp. Strain AI002. Both strains tolerated Cd up to 25 mg/L and produced 27–30 μg/mL of IAA. Inoculation of AI001 and AI002 improved plant growth dynamics (i.e., plant length, biomass, chlorophyll contents), relieved electrolyte leakage, and improved reduced glutathione significantly (P < 0.05). The inoculation of AI001 and AI002 significantly (P < 0.05) induced the expression of heavy metal ATPase genes ie., “HMA2, HMA3, and HMA4” and Cd translocation compared to uninoculated plants. Both AI001 and AI002 exhibited very prominent plant-growth-promoting and Cd phytoremediation properties. The results revealed that isolates also contributed a lot to the expression of rice plant heavy metal ATPase genes and in the Cd translocation in the plant.