Increase of P and Cd bioavailability in the rhizosphere by endophytes promoted phytoremediation efficiency of Phytolacca acinosa

Abstract

The information about the spatial distribution of bioavailable phosphorus (P) and heavy metal (HM) in the rhizosphere could aid in the precise phytoremediation regulation. In this study, a rhizobox system was adapted to study soil-root interactions and used to access the endophyte inoculation variation on bioavailable P and cadmium (Cd) spatial distribution during phytoremediation of Cd contaminated soils. Results showed that endophyte PE31 Bacillus cereus inoculation enhanced Cd uptake of P. acinosa by 52.70% and 46.73% in low and high Cd contaminated soils, increasing the phytoremediation hotspot area from 45.78% and 15.29% to 60.97% and 21.80%, respectively. Available P and Cd significantly diminished because root activities depleted large amounts of bioavailable P and Cd concentrations. However, PE31 increased bioavailable P and Cd concentration in the rhizosphere soil. The bioavailable P enhancement in the rhizosphere was positively correlated to plant growth and Cd accumulation. Overall, endophyte inoculation compensated the diminution of bioavailable P and Cd in the rhizosphere to improve plant biomass and HM absorption, and thus promote phytoremediation efficiency. This study helped to better understand bioavailable P and Cd spatial distribution under endophyte inoculation, which could provide effective management strategies for the precise regulation of phytoremediation.