Heating Block Research Articles

Oil-based drilling cutting pyrolysis residues- phosphogypsum foamed ceramics: Basic properties, microsintering mechanism and heavy metals solidification

Oil-based drilling cutting pyrolysis residues(ODPR) and phosphogypsum(PG) are typical types of industrial solid waste in China, characterized by low utilization rate and high output. The large amount of untreated accumulation will release heavy metals(HMs) and other pollutants, posing risks to the environment. Therefore, there is an urgent need to develop a high value-added building material product that can reduce environmental hazards while improving utilization rate. Based on this background, this study uses ODPR and PG as raw materials to explore their potential applications in the field of foamed ceramics. Research has shown that different sintering processes have a significant impact on the appearance and performance of products. By adjusting the preparation process, foamed ceramic products with excellent performance can be prepared. When the sintering temperature is 925℃, the foaming agent dosage is 3 %, and the raw material ratio ODPR:PG:Borax=35:45:20, a foamed ceramic product with a porosity of 60.95 %, water absorption rate of 3.4 %, compressive strength of 4.8 MPa, thermal conductivity of 0.8016 W/(m·k), and acid and alkali resistance of 98.57 % and 99.44 % can be prepared. The product meets the relevant requirements of “Fired heat preservation brick and block (GB/T 26538)” and “ General Specification for porous ceramic products (GB/T 16533)”. The HMs content test showed that the Ni and Pb contents in the pre-sintered blanks were the highest, at 0.6308 mg/kg and 0.3182 mg/kg, respectively. However, after high-temperature calcination, the solidification rate of HMs could reach up to 98.93 %, and the leaching amount was significantly reduced. The three further evaluation methods indicate that HMs in the pre-sintered blanks and foamed ceramic products do not pose significant ecological risks. The research results provide a green inorganic insulation material in the field of solid waste control and building materials.

Use of CO2 for enhanced carbon recovery in thermochemical processing of fruit peel waste

To address a supply variability associated with biomass, utilization of food waste provides a practical approach to consistently obtain carbon–neutral energy resource. Despite the technical merits of conventional biological process, the inevitable generation of less useful by-products, such as carbon dioxide (CO2) and sludge, remains a primary constraint. As an alternative, thermochemical process, such as pyrolysis, presents a promising solution for the comprehensive valorisation of food waste. In this study, citrus peel, one of the fruit processing wastes, was used as a model food waste. To enhance the eco-friendliness of the pyrolysis system, CO2 was introduced as a medium gas given the uncertainty regarding its behaviour as an inert or reactive agent at the pyrolysis temperatures. The pyrolysis approach demonstrated that CO2 interacts with volatile pyrogenic products, resulting in an increased formation of carbon monoxide (CO). Notably, this enhancement was observed at ≥ 400 °C, suggesting that CO2′s reactivity is limited at lower temperatures. To maximize the utility of CO2, the pyrolysis setup was modified by incorporating a heating block at 600 °C and a nickel-based catalyst (Ni/Al2O3). While these adjustments accelerated the thermal cracking of citrus peel, test setup modification led to an aromaticity increase of the resulting pyrogenic products. However, integrating CO2 into the catalytic pyrolysis created favourable conditions for detoxifying these aromatic compounds and further boosting CO production. Syngas productivity from catalytic pyrolysis in the presence of CO2 was 35.2 wt%, showing 3.67 times increase over that from conventional pyrolysis. In conclusion, CO2-assisted catalytic pyrolysis of the citrus peel offers significant technical benefits: it enhances carbon recovery in the form of CO and reduces the formation of undesirable aromatic pyrogenic products.

Using the Groundwater Cooling System and Phenolic Aldehyde Isolation Layer on Building Walls to Evaluation of Heat Effect

This study examines the thermal performance of building walls under full sunlight conditions using various insulation strategies. Specifically, it evaluates: (1) the effects of heat on building walls and indoor spaces; (2) the impact of groundwater cooling systems on thermal environments; (3) the influence of phenolic aldehyde insulation layers on heat transfer; and (4) the combined effects of groundwater cooling and phenolic aldehyde thermal insulation. Fluent–CFD (Computational Fluid Dynamics) was used in the study to simulate temperature transmission between the sun, the groundwater cooling system, and both indoor and outdoor spaces. Experimental analysis and simulations reveal that both the phenolic aldehyde insulation layer and the groundwater cooling system effectively reduce heat transfer, with the groundwater cooling system demonstrating the most significant impact. The phenolic aldehyde layer decreases the temperature difference between inner and outer walls by approximately 8 °C. The groundwater cooling system further reduces both inner and outer wall temperatures, helping to maintain cooler indoor environments. Simulation results indicate that, while the phenolic aldehyde layer effectively prevents external heat from penetrating into the room, it does not eliminate heat accumulation. In contrast, the groundwater cooling system efficiently dissipates heat, mitigating this issue. Groundwater analysis shows that maximum temperature differences occur at specific times of the day, with water flow effectively cooling the space. The combined use of the phenolic aldehyde insulation layer and the groundwater cooling system offers superior thermal performance. The phenolic layer provides effective heat blocking, while the groundwater system facilitates heat dissipation, optimizing indoor temperature and reducing air conditioning loads. This combination enhances overall comfort and energy efficiency, with the groundwater cooling system benefiting from reduced flow velocity and lower energy consumption.

Evaluation of an electricity-independent method for IS2404 Loop-mediated isothermal amplification (LAMP) diagnosis of Buruli ulcer in resource-limited settings.

Buruli ulcer (BU) caused by Mycobacterium ulcerans (MU) is a devastating necrotic skin disease. PCR, recommended for confirmation of BU by WHO, requires an adequately equipped laboratory, therefore often delaying timely diagnosis and treatment of BU patients in remote settings. Loop-mediated isothermal amplification (LAMP) is a PCR-based protocol for isothermal amplification of DNA that has been suggested for diagnosis of BU in low-resource settings. This is an exploratory diagnostic test evaluation study, with an embedded qualitative sub-study. Its aims are two-fold: First, to evaluate a simple rapid syringe-based DNA extraction method (SM) in comparison with a more elaborate conventional DNA extraction method (CM), followed by a LAMP assay targeting IS2404 for the detection of MU, either using a commercially available pocket warmer (pw) or a heat block (hb) for incubation. Second, to complement this by exploring the diagnostic workflow for BU at a community-based health centre in an endemic area in rural Ghana as an example of a potential target setting, using interviews with researchers and health care workers (HCWs). Diagnostic test evaluation results are discussed in relation to the requirements of a target product profile (TPP) for BU diagnosis and the target setting. A protocol using SM for DNA extraction followed by IS2404 PCR (IS2404 PCRSM) was able to identify MU DNA in 73 out of 83 BU clinical specimens submitted for diagnosis. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IS2404 PCRSM were 90.12%, 100%, 100% and 65.21% respectively, as compared to the reference standard IS2404 PCR in combination with a standard extraction protocol for mycobacterial DNA. Evaluation of the LAMP assay on 64 SM DNA extracts showed a sensitivity, specificity, PPV and NPV of 83.6%, 100%, 100% and 50%, respectively, using either pocket warmer (pwLAMPSM) or heat block (hbLAMPSM) for incubation of the reaction, as compared to the same reference standard. The limit of detection of pwLAMPSM was found to be 30 copies of the IS2404 target. Interview findings explored barriers to BU diagnosis and treatment, including perceptions of the disease, costs, and availability of transport. Participants confirmed that a diagnosis at the PoC, in addition to screening based on clinical criteria, would be advantageous in order to prevent delays and loss to follow-up. The high diagnostic and analytic accuracy of the pwLAMP, evaluated by us in combination with a syringe-based DNA extraction method, supports its potential use for the rapid detection of MU in suspected BU samples at the community or primary health care level without reliable electricity supply. Further optimization needs include a lysis buffer, evaluation directly at the PoC and/or other sites, assessing staff training requirements and quality control.

(Invited) Engineering the Switching Kinetics of Valence Change-Based Memristive Devices for Neuromorphic Computing

Memristive devices based on the valence mechanism are highly interesting candidates for the use as hardware representation of synapses in neuromorphic computing. The memristive model system SrTiO3 exhibit gradual switching and can be tuned between short-term and long-term plasticity. We will discuss the impact of the switching kinetics on the gradual switching mode and provide general guidelines for the design of gradual switching systems. Moreover, we present an approach to accelerate the switching kinetics of SrTiO3 by up to 103 times, or reduce the operating voltage by ≈ 30% to maintain the switching speed.Our approach is to introduce a low thermal conductivity layer inside the active electrode of the active electrode of the devices, which blocks the heat dissipation caused by Joule heating during switching. Our method leaves the switching layer and its interfaces with the electrodes intact, while the use of HfO2 and TaOx as the heat blocking layers ensures ease of fabrication and CMOS compatibility. We will demonstrate that this approach is transferable to other more common material systems.

Rapid Detection of Viral, Bacterial, Fungal, and Oomycete Pathogens on Tomatoes with Microneedles, LAMP on a Microfluidic Chip, and Smartphone Device.

Rapid detection of plant diseases before they escalate can improve disease control. Our team has developed rapid nucleic acid extraction methods with microneedles and combined these with loop-mediated amplification (LAMP) assays for pathogen detection in the field. In this work, we developed LAMP assays for early blight (Alternaria linariae, A. alternata, and A. solani) and bacterial spot of tomato (Xanthomonas perforans) and validated these LAMP assays and two previously developed LAMP assays for tomato spotted wilt virus and late blight. Tomato plants were inoculated, and disease severity was measured. Extractions were performed using microneedles, and LAMP assays were run in tubes (with hydroxynaphthol blue) on a heat block or on a newly designed microfluidic slide chip on a heat block or a slide heater. Fluorescence on the microfluidic chip slides was visualized using EvaGreen and photographed on a smartphone. Plants inoculated with X. perforans or tomato spotted wilt virus tested positive prior to visible disease symptoms, whereas Phytophthora infestans and A. linariae were detected at the time of visual disease symptoms. LAMP assays were more sensitive than PCR, and the limit of detection was 1 pg of DNA for both A. linariae and X. perforans. The LAMP assay designed for early blight detected all three species of Alternaria that infect tomato and is thus an Alternaria spp. assay. This study demonstrates the utility of rapid microneedle extraction followed by LAMP on a microfluidic chip for rapid diagnosis of four important tomato pathogens.

Enhanced Point-of-Care SARS-CoV-2 Detection: Integrating RT-LAMP with Microscanning.

The COVID-19 pandemic has highlighted the urgent need for rapid and accurate diagnostic methods for various infectious diseases, including SARS-CoV-2. Traditional RT-PCR methods, while highly sensitive and specific, require complex equipment and skilled personnel. In response, we developed an integrated RT-LAMP-MS assay, which combines rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) with microscanning (MS) technology for detecting SARS-CoV-2. The assay uses magnesium pyrophosphate formed during LAMP amplification as a visual marker, allowing direct observation via microscopy without the need for additional chemical indicators or probes. For the SARS-CoV-2/IC RT-LAMP-MS assay, the sample-LAMP reagent mixture was added to a microchip with SARS-CoV-2 primers and internal controls, then incubated at 62 °C for 30 min in a heat block, followed by amplification analysis using a microscanner. In clinical tests, the RT-LAMP-MS assay showed 99% sensitivity and 100% specificity, which is identical to the RT-LAMP results and comparable to the commercial AllplexTM SARS-CoV-2 assay results. Additionally, the limit of detection (LOD) was determined to be 10-1 PFU mL-1 (dynamic range: 103~10-1 PFU mL-1). The assay delivers results in 30 min, uses low-cost equipment, and demonstrates 100% reproducibility in repeated tests, making it suitable for point-of-care use in resource-limited settings.

Experimental investigation of a 10 kW-class flat-type loop heat pipe for waste heat recovery

A flat-type 10 kW-class loop heat pipe (LHP) with a box-type wick was designed and developed to handle the higher heat loads in waste heat recovery applications, such as industrial processing and internal combustion engines. Additionally, a numerical model was developed to predict the overall thermal performance of the LHP. The LHP used a stainless steel wick with a pore diameter of 2.0 µm and pure water as the working fluid. The LHP was tested using two types of cooling for the condenser (forced and natural convection), two types of evaporator orientations (horizontal and vertical), and with and without gravity assist (0.3 m and 0 m). For all the tests, the maximum heat load ranged from 5 kW to 10 kW, with the test with a gravity assist of 0.3 m, vertical evaporator orientation, and natural convection performing the best. This test sustained a 10 kW heat load at a steady-state temperature of 182 °C for the evaporator. During the same test, a maximum evaporative heat transfer coefficient of 92,000 W/m2/K at 4.5 kW and a thermal resistance between the evaporator and condenser value of less than 0.007 K/W was achieved. A numerical model was developed to compare the experimental results with the numerical temperature results for the heater block, evaporator, vapor line, condenser, liquid line, and compensation chamber. Overall, the average temperature difference for all components ranged from 7.1 °C to 10.6 °C, with the horizontal orientation without gravity assist and natural convection test predicting the best. The findings demonstrate that LHPs can handle the higher heat loads that are found in waste heat recovery applications for industrial processing and internal combustion engines.

Rapid and portable microfluidic chip-based qPCR for quality assurance of commercial seaweeds (lavers) in the supply chain

Lavers, an important type of seaweed rich in health-promoting substances, have seen a surge in global demand. However, their similar morphological characteristics among different species make them vulnerable to food fraud. To address this issue and ensure quality assurance, we developed a portable microfluidic quantitative polymerase chain reaction (qPCR) method to monitor six laver species, comparing its effectiveness with conventional qPCR. Our portable microfluidic qPCR system used a plate-shaped heat block for rapid heat transfer, combined with a microfluidic-based biochip for quick detection. The primers were specifically designed to target the chloroplast genes rbcL and rbcS. Specificity using 17 seaweed species showed no cross-reactivity, and the sensitivity of the assay was 1 × 10−4 ng. Furthermore, we enhanced the portable microfluidic qPCR for field applications by integrating it with a simple DNA extraction method. To validate the on-site rapid identification of laver species, we evaluated 79 laver samples collected from fish farms in Korea and commercially available laver products. We discovered instances where products were adulterated with cheaper species or replaced with others that resemble them. This method proves to be rapid, sensitive, reliable, and applicable for effectively managing and monitoring lavers in the supply chain.

Influence of the magnetic field on Al2O3-Cu/H2O hybrid nanofluid natural convection in a square cavity with heat sources and internal heat generation/absorption

In view of the growing interest in the numerical approach during the optimization phase of technical projects, this study proposes an in-depth analysis of the effects of several relevant parameters on the cooling of heat sources, part of the existing problems in the field of heat exchangers. The study focused on a natural convection problem inside a square cavity traversed by a vertical or horizontal magnetic field. This cavity contains four heating blocks, arranged at a uniform distance from each other and from the different surrounding walls. A hybrid nanofluid, composed of aluminum oxide (Al2O3) and copper (Cu) particles in equal proportions, was considered inside the cavity, imposing on it heat-generating or heat-absorbing behavior. The finite volume method was adopted as the solving approach for the adimensional governing equations, with the SIMPLE algorithm specifically chosen to handle the coupling between the momentum and continuity equations. Numerical results, covering a wide range of adimensional parameters such as Rayleigh number, Hartman number, volume fraction, internal heat value of the hybrid nanofluid, as well as for horizontal and vertical magnetic field orientations, have been grouped according to different scenarios to provide in-depth technical answers to fundamental questions related to the specific problem studied. These results have shown that increasing the flow regime leads to a transition from conductive to convective heat transfer, thus favoring an increase in heat exchange of up to 85% in certain scenarios. It is also clear that the cooling process of the heat sources is enhanced by the absorbing behavior of the hybrid nanofluid. Furthermore, the effects of the magnetic field (Ha) and the nanoparticle volume fraction (ϕ hnf) proved to be opposites: the former has an inhibiting effect, while the latter stimulates.

CHF and heat transfer enhancement by SiO2 nanofluids on a inclined downward facing heating surface

In this experiment, the factors affecting critical heat flux (CHF) in the pool boiling research with the heating face down were investigated. Including the type of cooling liquid and the angle of the heating surface. The types of coolants include reverse osmosis (R·O) water and SiO2 nanofluids (0.02 g/L～0.12 g/L). The morphology and types of nanoparticles were characterized by microscope (SEM) and scanning electron X-ray diffraction (XRD) before the experiment. Pool boiling experiments were performed using a specially designed heating block. After data processing, we found that in the horizontal downward heating experiment, the heat flux density increased by 49.68% and the heat transfer coefficient (HTC) increased by 60.73% under nanofluid conditions compared to the case of R·O water when CHF occurred. The increase in the HTC is strongly related to the deposition of nanoparticles on the heating surface. In the heating surface experiment with different tilt angles, both the heat flux density and the HTC increase with the increase of the angle. In the experiment of inclined downward heating surface, the CHF and HTC increase with the increase of heating angle, but the increase is smaller and smaller.

Multivariate Analysis of Okra [Abelmoschus esculentus (L.) Moench] Genotypes and Hybrids based on Mineral Content

Background: In 2021, the world production of okra [Abelmoschus esculentus (L.) Moench] was over 10.8 million tonnes which is led by India (about 60%). The objective of this study was to determine mineral content of genotypes and hybrids of okra identified by our in-house heterosis breeding programme. Methods: The samples were digested in a heating block using nitric acid and hydrogen peroxide and analyzed using inductively coupled plasma mass spectrometry (ICP-MS) for mineral profiling of okra samples. Result: The results were evaluated using multivariate analysis. The genotype EC169474 was with the highest concentration of minerals Na (184.91 μg/g), K (14613.52 μg/g) and Fe (268.76 μg/g), while, EC169477 was found to have highest concentration of Mg (6530.76 μg/g) and Ca (273.58 μg/g). Comparatively, in hybrids, EC 169470 x EC169474 contains highest concentration of minerals Na (177.27 μg/g) and Ca (253.01 μg/g), EC169474 x EC169477 contains highest Mg (6301.74 μg/g) and hybrid EC169468 x EC169477 contains highest K (14412.99 μg/g) and Fe (246.51 μg/g). Principal component analysis (PCA) reveals that the element that contributed most for the variability between the genotypes and hybrids was K, moderate contribution by Ca, Fe and Na, while, Mg being the least. Score plot of principal components reveals EC169470 and EC169477 among the most variable genotypes and EC169470 x EC169477 was the most variable hybrid. Hierarchical cluster analysis (HCA) tends to separate all analyzed samples in two major clusters. However, the genotypes EC169474 and EC169477 were grouped in hybrid cluster, while, hybrid EC169470 ´ EC169477 grouped with genotype cluster. The multivariate analysis revealed a systematic difference in the mineral content of okra. The results presented in this study are of great value for nutritionally dense breeding programmes for okra that provides important nutritional diversity by hybrids.

Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes

BackgroundS. pyogenes, is a primary pathogen that leads to pharyngitis and can also trigger severe conditions like necrotizing fasciitis and streptococcal toxic shock syndrome (STSS), often resulting in high mortality rates. Therefore, prompt identification and appropriate treatment of S. pyogenes infections are crucial in preventing the worsening of symptoms and alleviating the disease's impact. ResultsIn this study, a newly developed technique called multiple cross displacement amplification (MCDA) was employed to detect S. pyogenes,specifically targeting the speB gene, at a temperature of 63°C within 30 min. Then, an easily portable and user-friendly nanoparticles-based lateral flow biosensor (LFB) assay was introduced for the rapid analysis of MCDA products in just 2 min. The results indicated that the LFB offers greater objectivity compared to Malachite Green and is simpler than electrophoresis. The MCDA-LFB assay boasts a low detection limit of 200 fg and exhibits no cross-reaction with non-S. pyogenes strains. Among 230 clinical swab throat samples, the MCDA-LFB method identified 27 specimens as positive, demonstrating higher sensitivity compared to 23 samples detected positive by qPCR assay and 18 samples by culture. The only equipment needed for this assay is a portable dry block heater. Moreover, each MCDA-LFB test is cost-effective, priced at approximately $US 5.5.ConclusionThe MCDA-LFB assay emerges as a straightforward, specific, sensitive, portable, and user-friendly method for the rapid diagnosis of S. pyogenes in clinical samples.

The relation between Rossby wave-breaking events and low-level weather systems

Abstract. Rossby wave-breaking events describe the last stage in the life cycle of baroclinic atmospheric disturbances. These breaking events can strongly influence large-scale circulation and are also related to weather extremes such as heat waves, blocking, and extreme precipitation events. Nonetheless, a complete understanding of the synoptic-scale dynamics involved with the breaking events is still absent. For example, it is not clear how well the theoretical life cycle experiments, which use a specified initial perturbation with a single zonal wavenumber and a prescribed simplified initial zonal jet, capture the life cycle of real-atmosphere weather systems. Here we combine a storm-tracking technique together with a wave-breaking detection algorithm to examine how upper-level wave breaking and surface weather systems are related in the North Atlantic during winter. These datasets allow us to examine whether upper-level wave breaking and low-level weather systems always occur simultaneously and if we can identify preferred relations between the surface weather system type (cyclone or anticyclone) and the type of the upper-level breaking event (cyclonic or anticyclonic wave breaking denoted CWB or AWB, respectively). We find that in the North Atlantic, most weather systems are associated with AWB and/or CWB at some point during their lifetime, while only few cyclones and anticyclones do not involve any upper-level wave breaking (roughly 11 % and 15 %, respectively). Our results imply that composites of cyclones and anticyclones involve a mixture of different types of life cycles, depending on whether they involve CWB or AWB, as well as their position relative to the Rossby wave-breaking (RWB) center. Moreover, the system characteristics (including actual and relative positions, intensities, and displacements) differ depending on the associated breaking type. We distinguish between “same-pairing” cases (i.e., cyclone with CWB and anticyclones with AWB) and “opposite-pairing” cases (i.e., cyclones with AWB and anticyclones with CWB). Compositing the cyclones and anticyclones based on this criterion, we find that in similar pairings the surface system is positioned so that its associated upper-level winds would enhance the breaking (the anomalous circulation is in the same direction as the background shear), but, for opposite pairings, the upper-level winds associated with the surface system do not act to enhance the breaking which occurs in the direction of the background shear. A better understanding of the different life cycles of real-atmosphere cyclones and anticyclones and the upper-level breaking they involve is important for exploring the relation between storm tracks and slowly varying weather regimes and how they are mediated by RWB events.

Disease Progress and Detection of a California Resistance-Breaking Strain of Tomato Spotted Wilt Virus in Tomato with LAMP and CRISPR-Cas12a Assays

Use of tomato cultivars with the Sw-5 resistance gene cluster has led to the occurrence of resistance-breaking (RB) tomato spotted wilt virus (TSWV) strains globally, including California and, recently, North Carolina and Texas. We documented disease on tomato infected with either an RB strain from California (CA-RB) or a wild type (CA-WT) strain of TSWV on tomato with (cultivar Mountain Merit) or without (cultivar Mountain Fresh Plus) the Sw-5b resistance gene and detected virus incidence over time using microneedle RNA extractions and LAMP. We developed a LAMP/Cas12a assay for detection of the CA-C118Y mutation in a CA-RB strain and tested the assay with field samples. Disease in the susceptible cultivar was less severe with CA-RB than with the CA-WT strain. In contrast, the resistant cultivar had little disease when inoculated with the CA-WT strain but exhibited stunting of greater than 50% when inoculated with the CA-RB strain. In the susceptible tomatoes, the detection rates over time by LAMP reaction were higher in CA-WT than in CA-RB-inoculated plants. In resistant tomato, CA-RB remained detectable by TSWV LAMP over 14 days, whereas the WT strain was undetectable. A two-step LAMP/Cas12a assay differentiated the two strains in 1 h. Our methods were validated with samples from TSWV-infected North Carolina fields. A phylogeny of NSm gene sequences that included North Carolina field samples revealed two independent origins of the North Carolina RB isolates. The LAMP/Cas12 assay showed excellent detection of the CA-C118Y mutation. The TSWV LAMP/Cas12a assay is adaptable for in-field applications on either a smart phone platform or heat block. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Thermal Test Apparatus for Mini Heat Sinks

Abstract Demands for more powerful and smaller electronic devices have increased the energy dissipation requirements. Accurate determination of the thermal performance of small sized heat sinks is necessary for innovation within the heat dissipation sector. This study designed, developed, and tested an apparatus for determining the thermal performance of mini heat sinks (MHS). The test apparatus consisted of a wind tunnel, fan, heater, heater block, five temperature sensors, air velocity sensor, and a data acquisition system. A robust dataset was created by testing the heater without a MHS and testing two different MHS materials of polycarbonate (PC) and aluminum (AL) and having 16 to 21 repeat tests. Linear and polynomial approximations for the temperature profile were explored. For the steady state tests, the mean and 90% confidence interval were calculated to determine statistically significant differences. The temperature gradient at the interface, rate of heat transfer, and the thermal resistances from the polynomial fit had higher variation than the linear fit. The experimentally determined heater surface temperature had a 90% confidence interval of ±0.3 to ±0.7°C. The 90% confidence intervals for the thermal resistances were 1.0 to 1.5 K/W for linear and 2.3 to 6.0 K/W for polynomial. Statistically significant differences for the temperature gradient at the interface, rate of heat transfer, and thermal resistances between the bare, PC, and AL were found. Due to heat losses, the linear fit had greater precision, but the polynomial fit had greater accuracy.

End-point rapid detection of total and pathogenic Vibrio parahaemolyticus (tdh+ and/or trh1+ and/or trh2+) in raw seafood using a colorimetric loop-mediated isothermal amplification-xylenol orange technique.

Vibrio parahaemolyticus is the leading cause of bacterial seafood-borne gastroenteritis in humans worldwide. To ensure seafood safety and to minimize the occurrence of seafood-borne diseases, early detection of total V. parahaemolyticus (pathogenic and non-pathogenic strains) and pathogenic V. parahaemolyticus (tdh+ and/or trh1+ and/or trh2+) is required. This study further improved a loop-mediated isothermal amplification (LAMP) assay using xylenol orange (XO), a pH sensitive dye, to transform conventional LAMP into a one-step colorimetric assay giving visible results to the naked eye. LAMP-XO targeted rpoD for species specificity and tdh, trh1, and trh2 for pathogenic strains. Multiple hybrid inner primers (MHP) of LAMP primers for rpoD detection to complement the main primer set previously reported were designed by our group to maximize sensitivity and speed. Following the standard LAMP protocol, LAMP reaction temperature for rpoD, tdh, trh1, and trh2 detection was first determined using a turbidimeter. The acquired optimal temperature was subjected to optimize six parameters including dNTP mix, betaine, MgSO4, Bst 2.0 WarmStart DNA polymerase, reaction time and XO dye. The last parameter was done using a heat block. The color change of the LAMP-XO result from purple (negative) to yellow (positive) was monitored visually. The detection limits (DLs) of LAMP-XO using a 10-fold serial dilution of gDNA and spiked seafood samples were determined and compared with standard LAMP, PCR, and quantitative PCR (qPCR) assays. Subsequently, the LAMP-XO assay was validated with 102 raw seafood samples and the results were compared with PCR and qPCR assays. Under optimal conditions (65°C for 75 min), rpoD-LAMP-XO and tdh-LAMP-XO showed detection sensitivity at 102 copies of gDNA/reaction, or 10 folds greater than trh1-LAMP-XO and trh2-LAMP-XO. This level of sensitivity was similar to that of standard LAMP, comparable to that of the gold standard qPCR, and 10-100 times higher than that of PCR. In spiked samples, rpoD-LAMP-XO, tdh-LAMP-XO, and trh2-LAMP-XO could detect V. parahaemolyticus at 1 CFU/2.5 g spiked shrimp. Of 102 seafood samples, LAMP-XO was significantly more sensitive than PCR (P < 0.05) for tdh and trh2 detection and not significantly different from qPCR for all genes determined. The reliability of tdh-LAMP-XO and trh2-LAMP-XO to detect pathogenic V. parahaemolyticus was at 94.4% and 100%, respectively. To detect total and pathogenic V. parahaemolyticus, at least rpoD-LAMP-XO and trh2-LAMP-XO should be used, as both showed 100% sensitivity, specificity, and accuracy. With short turnaround time, ease, and reliability, LAMP-XO serves as a better alternative to PCR and qPCR for routine detection of V.parahaemolyticus in seafood. The concept of using a one-step LAMP-XO and MHP-LAMP to enhance efficiency of diagnostic performance of LAMP-based assays can be generally applied for detecting any gene of interest.

Numerical investigation of mixed convection inside a three-dimensional l-shaped cavity filled with hybrid-nanofluids in the presence of a heating block

Numerical investigation of mixed convection inside a three-dimensional l-shaped cavity filled with hybrid-nanofluids in the presence of a heating block

Loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor for monkeypox virus detection

Monkeypox virus (MPXV) infection is currently an evolving public health concern, highlighting an urgent need for early and rapid detection of MPXV. Here, we present a diagnostic test called MPXV-LAMP-LFB, which combines loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the simple, sensitive and specific detection of MPXV and differentiation of its two clades. The MPXV-LAMP-LFB can be conducted at a heating block and the detection results can be visually indicated with the biosensor without any specialized apparatus. Two sets of LAMP primers targeting the D14L and ATI genes were designed for the Central and West African MPXV isolates, respectively. The optimal amplification condition was 64 °C for 40 min. Thus, the MPXV-LAMP-LFB test can be completed within 1 h, incorporating rapid DNA extraction (∼15 min), LAMP reaction (∼40 min) and result indicating (∼5 min). The MPXV-LAMP-LFB assay could detect down to 5 copies of plasmid template and 12.5 copies of pseudotyped virus in simulated blood samples. Furthermore, the MPXV-LAMP-LFB assay correctly identified all the positive controls and successfully avoided cross-reactivity with the non-MPXV pathogens or clinical samples, demonstrating its high specificity. Overall, the MPXV-LAMP-LFB test developed in this study showed great promise as a rapid, sensitive and accurate molecular tool for diagnosing MPXV infection.

Comparative genomic analysis of Mycoplasma related to cell culture for infB gene-based loop-mediated isothermal amplification.

Mycoplasma contamination in cell culture affects the properties of cell lines. Gold standard detection by microbiological culture takes days and requires specialists. The polymerase chain reaction and loop-mediated isothermal amplification (LAMP) are fast molecular options, but LAMP only requires one heating block for DNA amplification. This study presents a comparative genomic analysis of Mycoplasma species to identify common target genes different from the rrsA gene, which encodes 16S rRNA. The aim is to implement a LAMP assay to detect Mycoplasma species, reducing the time and specialized equipment required for detection. We performed a comparative genomic analysis through Mauve software and the GView server and selected infB and clpB genes as target candidates for designing LAMP primers. We evaluated both genes by multiple sequence alignment (MSA). The infB gene presented the best score MSA assessment with lower odd-log values (5,480,281) than other genes. We selected the infB gene to design LAMP primers specific to Mycoplasma spp. We used these primers to implement LAMP at 63°C for 30min, which showed 100% positive amplifications for detecting Mycoplasma spp. In conclusion, we present a methodology utilizing the infB gene-based LAMP assay to detect three of the six most prevalent Mycoplasma species in cell culture.