What is the central question of this study? Do haemoglobin mass and red blood cell volume increase in elite cyclists training in a hot environment compared to a control group training at normal temperature? What is the main finding and its importance? Fiveweeks of heat training increases haemoglobin mass in elite cyclists. There are small to intermediate effect sizes for exercise parameters favouring heat training. In this study we tested the hypothesis that performing 1h of regular light exercise in a heat chamber (HEAT; 37.8±0.5°C; 65.4±1.8% humidity) 5timesweek-1 for a total of 5weeks increases haemoglobin mass (Hbmass ) and exercise performance in elite cyclists ( =76.2±7.6mlmin-1 kg-1 ). Twenty-three male volunteers were assigned to HEAT (n=11) or CON (n=12; 15.5±0.1°C; 25.1±0.0% humidity) training groups. Hbmass was determined before and after the intervention period in conjunction with an extensive exercise test protocol (conducted at 16-19°C). HEAT increased (P<0.05) Hbmass by 42g from 893±78 to 935±108g whereas Hbmass remained unchanged (+6g) in CON. Furthermore, statistical analysis revealed a time-group interaction (P<0.05). The greater increase in Hbmass in HEAT, however, did not manifest in a greater increase in (225±274mlmin-1 in HEAT and 161±202mlmin-1 in CON). While HEAT reduced (P<0.05) lactate levels during some of the submaximal exercise tests, there was no statistical difference between other performance parameters. There were, however, small to intermediate effect sizes favouring HEAT for lactate threshold power output (2.8±3.9 vs. -0.4±5.1% change, effect size (ES)=0.34), gross economy in the fatigued state (0.19±0.42 vs. -0.12±0.49%-point change, ES=0.52) and 15min mean power (6.9±8.4vs. 3.4±5.1% increase, ES=0.22). This study demonstrates an increase in Hbmass and small to intermediate effect sizes on exercise variables in elite cyclists following a 5-week heat training intervention.