Event Abstract Back to Event Study of the impact of glycation in wound healing using a tissue-engineered skin Kiefer K. Thouin1, Sébastien S. Cadau1 and François F. Berthod1 1 Laval University, Surgery, Canada The wound healing process is essential to restore the skin barrier function to prevent fluid loss and infection. The peripheral nervous system plays an important role in this process by inducing neurogenic inflammation. Diabetes causes neuropathy that aggravates wound healing closure, leading to ulcer development and high risks of lower limb amputation. This process is mainly due to diabetic-induced hyperglycemia causing tissue glycation and advanced glycated endproduct (AGE) formation such as N-carboxymethyl-lysine. Our hypothesis is that the deleterious effects of AGEs in wound healing which are observed in diabetes could be reversed by a treatment with anti-AGE and AGE-breaker molecules. The project’s aim is to develop an in vitro wound within glycated tissue-engineered reconstructed skin to evaluate the influence of AGEs on the reepithelialisation process and to develop a topical treatment targeting AGEs to improve it. Method: We developed an in vitro wound-healing model by using a biomaterial made of collagen and chitosan to create a reconstructed skin in which an 8mm diameter wound is created at the epidermal level. The treatment of this model with glyoxal started one week before the wound was made and was maintained for three weeks to induce expression of AGEs and recapitulate the glycation process. We have evaluated the effect of a treatment with aminoguanidin, an anti-AGE molecule, and alagebrium, an AGE-breaker molecule, to establish if these molecules could improve different parameters in glycated wound healing. Results and Conclusions: Results suggest that the tissue engineered skin we used is a good model to recapitulate the diabetic wound healing process and suggest that the action of aminoguanidin and alagebrium partially inhibits AGEs production and improves the reepithelialisation of the wound in glycated models. Thus, their topical application on ulcers could be a valuable approach to improve wound healing in diabetic patients with minimal systemic side effects. François Berthod; Sabrina Bellenfant; Sébastien Cadau; Lorène Mottier; François Auger; IRSC; LOEX; Medecine faculty of Laval University Keywords: Regenerative Medicine, Tissue Engineering, biomaterial, matrix-cell interaction Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Thouin KK, Cadau SS and Berthod FF (2016). Study of the impact of glycation in wound healing using a tissue-engineered skin. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02420 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kiefer K Thouin Sébastien S Cadau François F Berthod Google Kiefer K Thouin Sébastien S Cadau François F Berthod Google Scholar Kiefer K Thouin Sébastien S Cadau François F Berthod PubMed Kiefer K Thouin Sébastien S Cadau François F Berthod Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.