Antioxidant Effect of Channa Micropeltes in Diabetic Wound of Oral Mucosa

Abstract

Background and Objective: Toman fish (Channa micropeltes) contains albumin which possesses antioxidant effect. People in south Kalimantan, Indonesia, use toman fish as an alternative drug to accelerate diabetic wound healing process. In diabetic wound, there is a reduction in superoxide dismutase (SOD) activity and an elevation in malondialdehyde (MDA) level which can lead to the occurrence of delayed wound healing. The objective of this study was to prove the antioxidant effect of Channa micropeltes using SOD activity and MDA level in diabetic wound of oral mucosa. Materials and Methods: This is an experimental study with post test-only control group design. This study used 24 Wistar rats which divided into three treatment groups. The groups comprised of one group administered with toman fish extract of 16 mL kg–1 rat BW per oral, one group as a positive control administered with Haruan fish extract of 13.54 mL kg–1 rat BW per oral and one group as a negative control given BR2 feed only. The SOD activity measurement was done through Misra and Fridovich method using spectrophotometer in 480 nm wavelength. The MDA level measurement was done through Thiobarbituric Acid (TBA) test from Buege and Aust method using spectrophotometry in 532 nm wavelength. Results: One-way ANOVA test of day 3 showed no significant difference in SOD activity (p = 0.078) and MDA level (p = 0.094). Meanwhile, statistical result of day 7 showed significant difference in SOD activity (p = 0.018) and MDA level (p = 0.011). Post-hoc LSD test showed significant difference between toman fish extract of 16 mL kg–1 rat BW dosage and haruan fish extract of 13.54 mL kg–1 rat BW dosage and group given BR2 feed only. Conclusion: It can be concluded that Channa micropeltes has an antioxidant effect which represented by the increase of SOD activities and the decrease of MDA level in diabetic wound of oral mucosa