Healing Process Of Burns Research Articles

Effects of herbal ointment containing the leaf extracts of Madeira vine (Anredera cordifolia (Ten.) Steenis) for burn wound healing process on albino rats.

Aim:Skin burn is a health problem that requires fast and accurate treatment. If not well-treated, the burn will cause various damaging conditions for the patient. The leaf extract of Madeira vine (Anredera cordifolia (Ten.) Steenis), or popularly known as Binahong in Indonesia, has been used to treat various diseases. The purpose of this research is to determine the effects of leaf extracts of Madeira vine (A. cordifolia (Ten.) Steenis) on skin burn healing process in rats as an animal model.Materials and Methods:In this research, there were four treatment groups: G0, G1, G2, and G3, each consisting of five rats. All these rats were given skin burns, using hot metal plates. Then, sulfadiazine was given to G0, 2.5% leaf extract of Madeira vine was given to G1, 5% extract was given to G2, and 10% extract was given to G3, for straight 14 days topically, 3 times a day. At the end of the treatment period, skin excisions were conducted, and histopathological examination was carried out.Result:Microscopic observation on the wound healing process on the collagen deposition, polymorphonuclear infiltration, angiogenesis, and fibrosis showed that G2 had a significant difference with G0, G1, and G3 (p<0.05), while group G0 was significantly different from G1 and G3 (p<0.05). The better burn healing process on G2 allegedly because of the activity of flavonoid, saponin, and tannin, contained in the Madeira vine, which have the antioxidant, anti-inflammatory, and antibacterial effects.Conclusion:The ointment from the 5% leaf extract of Madeira vine (A. cordifolia (Ten.) Steenis) has been proven to be effective to be used for topical burn therapy.

UJI AKTIVITAS EKSTRAK ETANOL GAMBIR (Uncaria gambir Roxb.) TERHADAP PENYEMBUHAN LUKA BAKAR PADA KULIT PUNGGUNG MENCIT PUTIH JANTAN (Mus musculus)

Gambir (Uncaria gambir Roxb.) contains flavonoids, tannins, saponins and alkaloids that can help in the healing process of burns on the back skin of mice. Gambir has been used for the treatment because it has the effect of antimicrobial and anti-inflammatory. Gambir is used by the people as a traditional medicine to treat burns.This research is experimental research. The object studied is the potential of gambir extract on healing burns of mice back skin. The number of mice used are 15 which divided into 5 groups: the positive control group (branded ointment), negative control group (vaseline flavum), group of gambir ethanol extract and vaseline flavum concentration of 25%, 35%, and 45%. Each group consisted of 3 mice.Backs of the mice induced using a heated solder which tip contained stainlees plate measuring 1x1 cm and placed for 2 seconds on the back skin of mice. The percentage of burns healing is obtained by calculating the surface area of the wound. The data were analyzed using ANOVA analysis followed by LSD test. The results showed that ethanol extract of gambir concentration of 25% with the healing burns percentage of (72.00%), the concentration of 35% (85.00%), and the concentration of 45% (88.67%) have activity on healing burns of back skin of mice. The results of data analysis using ANOVA showed that the data has significant differences with a significance value &lt;0.05 is 0.000. LSD test results stated that each treatment group have significant differences with a significance value &lt;0.05. It can be seen that with increasing dose will be followed by duration of burns healing

ЗМІНИ БІЛКОВОГО СКЛАДУ СИРОВАТКИ КРОВІ ТА ГОМОГЕНАТУ СТРАВОХОДУ ПРИ РОЗВИТКУ КИСЛОТНОГО ОПІКУ СТРАВОХОДУ СТАТЕВОНЕЗРІЛИХ ЩУРІВ

The steady increase of the number of chemical esophagus burns is noted over the past tenth anniversary. The esophageal acid burns are seen frequently in children ages 1 to 8 years. The chemical injuries sights are characterized by increase synthesis and degradation of proteins and scar formation. The cicatricial changes of the esophagus, developing as a result of burns, remain one of the most difficult problems of physiological functions maintaining. Despite of numerous studies, there are many insufficiently studied issues of esophagus burn healing and scarring process at the cellular and molecular level. Further study of the esophageal burns healing process would be useful to create effective approaches to prevention of scarring formation in esophagus.

Effect of Allogeneic Bone Marrow-mesenchymal Stem Cells (BM-MSCs) to Accelerate Burn Healing of Rat on the Expression of Collagen Type I and Integrin α2β1.

Burn is a public health problem, it causes physical disability even death. Treatment of burn wound has been conducted in various ways, but the satisfactory healing has not been provided. Bone marrow-derived mesenchymal stem cells (BM-MSCs) treatment is one of attempt to burn recovery, accelerate wound healing and angiogenesis. This study aimed to investigate the effect of allogeneic BM-MSC treatment on the expression of collagen type I and integrin α2β1 in burn skin tissue of rat observed on day 14. Twelve Wistar rats divided into two groups, control group (injected with phospate buffer solution) and treatment group (injected with BM-MSC). Rat was anaesthetized with xylazine and ketamine (ratio 1:1), fur of rat's back was shaved and full thickness burn was made by boiling plate in hot water for 30 min and patched on the back for 20 min. The burns were covered by tegaderm film and elastomult haft. Antalgin as an analgetic was injected to rats during observation process. Burns of rat was observed on day 14. In this study one-way analysis of variance test and Tukey as a further test were analyzed. The results showed that the healing time of allogeneic BM-MSC treatment on burn skin tissue rats was faster, the thickness of collagen type I in burn skin tissue of rats was thicker (0.977 μm) than controls (0.475 μm) and statistically demonstrated significant differences (p = 0.000). The average percentage of integrin α2β1 expression was higher (2.94%) than control group (2.34%), but the differences were not statistically significant (p = 0.176). The study concluded that BM-MSC treatment was able to accelerate the healing process of burns by increasing the thickness of the collagen and the percentage of integrin α2β1, thus accelerated the cell migration involved during wound healing.

In Vivo Monitoring of Microcirculation in Burn Healing Process with Optical Microangiography.

Objective: Optical microangiography (OMAG)-based optical coherence tomography is a noninvasive technique capable of imaging functional microvasculature innervating scanned tissue volume. In this study, we utilize OMAG to investigate dynamic changes of microcirculation during the healing process of a burn. Approach: A soft-contact superficial burn injury was induced on a mouse ear with 1 μL 70°C hot water. Microangiograms were generated by using OMAG before and after the burn. Results: Vessel recruitment and remodeling were observed in the healing process. Burn injury reached to the worst extent within the first 24 h and had no expansion thereafter. The interrupted microcirculation in the mouse ear was progressively recovered in the consequent postburn days and completely healed on postburn day 7. Innovation: OMAG provides a novel way for noninvasive visualization and quantification of vasculature changes over time after burn injuries. The high resolution achieved by the imaging system reveals microvascular details down to capillary level. Conclusion: Our results demonstrated that OMAG has great potential to improve the understanding of microcirculatory responses to burns and thus benefit the development of effective therapeutics.

Fumarate-loaded electrospun nanofibers with anti-inflammatory activity for fast recovery of mild skin burns

In the biomedical sector the availability of engineered scaffolds and dressings that control and reduce inflammatory states is highly desired, particularly for the management of burn wounds. In this work, we demonstrate for the first time, to the best of our knowledge, that electrospun fibrous dressings of poly(octyl cyanoacrylate) (POCA) combined with polypropylene fumarate (PPF) possess anti-inflammatory activity and promote the fast and effective healing of mild skin burns in an animal model. The fibers produced had an average diameter of (0.8 ± 0.1) µm and they were able to provide a conformal coverage of the injured tissue. The application of the fibrous mats on the burned tissue effectively reduced around 80% of the levels of pro-inflammatory cytokines in the first 48 h in comparison with un-treated animals, and enhanced skin epithelialization. From histological analysis, the skin thickness of the animals treated with POCA : PPF dressings appeared similar to that of one of the naïve animals: (13.7 ± 1.4) µm and (14.3 ± 2.5) µm for naïve and treated animals, respectively. The density of dermal cells was comparable as well: (1100 ± 112) cells mm−2 and (1358 ± 255) cells mm−2 for naïve and treated mice, respectively. The results demonstrate the suitability of the electrospun dressings in accelerating and effectively promoting the burn healing process.

Aged garlic extract ameliorates immunotoxicity, hematotoxicity and impaired burn-healing in malathion- and carbaryl-treated male albino rats.

Malathion and carbaryl are the most widely used organophosphate and carbamate insecticides, respectively, especially in developing countries; they pose a potential health hazard for both humans and animals. Here, we evaluated the protective effects of an odorless (free from allicin) Kyolic aged garlic extract (AGE, containing 0.1% S-allylcysteine; 200 mg/kg body weight) on the toxicity induced by 0.1 LD50 of malathion (89.5 mg/kg body weight) and/or carbaryl (33.9 mg/kg body weight) in male Wistar rats. Doses were orally administered to animals for four consecutive weeks. The present study showed that AGE completely modulated most adverse effects induced by malathion and/or carbaryl in rats including the normocytic normochromic anemia, immunosuppression, and the delay in the skin-burning healing process through normalizing the count of blood cells (erythrocytes, leucocytes and platelets), hemoglobin content, hematocrit value, blood glucose-6-phosphodehydrogenase activity, weights and cellularity of lymphoid organs, serum γ-globulin concentration, and the delayed type of hypersensitivity response to the control values, and accelerating the inflammatory and proliferative phases of burn-healing. In addition, AGE completely modulated the decrease in serum reduced glutathione (GSH) concentration and the increase in clotting time in malathion alone and carbaryl alone treated rats. Moreover, AGE induced a significant increase (P < 0.001) in serum GSH concentration (above the normal value) and accelerating burn-healing process in healthy rats. In conclusion, AGE was effective in modulating most adverse effects induced in rats by malathion and carbaryl, and hence may be useful as a dietary adjunct for alleviating the toxicity in highly vulnerable people to insecticides intoxication. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 789-798, 2017.

4. EFIKASI MENTIMUN (Cucumis sativus L.) TERHADAP PERCEPATAN PENYEMBUHAN LUKA BAKAR (Vulnus combustion) DERAJAT IIB PADA TIKUS PUTIH (Rattus norvegicus) (Efficacy of Cucumber (Cucumis sativus L.) on Healing of IIB Degree Burn Wound (Vulnus combustion) in Rat (Rattus norvegicus))

This research was aimed to determine the efficacy of cucumber (Cucumis sativus L.) on healing of burn wound IIB degree in rat (Rattus norvegicus). Experimental animal used were 6 male rats, aged 2-3 months with average weight of 150-250 grams. Rats were divided into 2 treatment groups, each group consist of 3 rats. Burn wound was made on the dorsal of the rats using a solder that has been linked with 2 cm diameter metal and heated for 5 minutes. The metal was placed for 5 seconds on each rat tail to form IIB degree burns. The burn wound were observed and treated according to the treatment group everyday. Group 1 as a negative control was smeared with aquades and group II was given the cucumber. Treatment of burn wound was conducted twice daily in the morning and evening until the diameter of the burn wound closed. Data were analyzed using independent t test. Statistical analysis showed that the healing of burn wound using a cucumber have no significant effect (P0.05) on reducing of burn wound diameter, red wound color and edema, scab formation and epithelization. This result can be concluded that the cucumber could not enhance the healing process of IIB degree wound burn on rats. ____________________________________________________________________________________________________________________ Key words: cucumber, burn wound, rat

Improved burn wound healing by the antimicrobial peptide LLKKK18 released from conjugates with dextrin embedded in a carbopol gel

Antimicrobial peptides (AMPs) are good candidates to treat burn wounds, a major cause of morbidity, impaired life quality and resources consumption in developed countries. We took advantage of a commercially available hydrogel, Carbopol®, a vehicle for topical administration that maintains a moist environment within the wound site. We hypothesized that the incorporation of LLKKK18 conjugated to dextrin would improve the healing process in rat burns. Whereas the hydrogel improves healing, LLKKK18 released from the dextrin conjugates further accelerated wound closure, and simultaneously improving the quality of healing. Indeed, the release of LLKKK18 reduced oxidative stress and inflammation (low neutrophil and macrophage infiltration and pro-inflammatory cytokines levels). Importantly, it induced a faster resolution of the inflammatory stage through early M2 macrophage recruitment. In addition, LLKKK18 stimulated angiogenesis (increased VEGF and microvessel development in vivo). Moreover, collagen staining evaluated by Masson’s Trichrome was visually much more intense after treatment with LLKKK18, suggesting higher collagen deposition.Overall, we generated an effective, safe and inexpensive formulation that maintains a moist environment in the wound, easy to apply and remove, and with potential to prevent infection due to the presence of an antimicrobial peptide. These findings propel us to further study this LLKKK18-containing formulation, setting the foundations towards a potential therapeutic approach for burn wound treatment. Statement of SignificanceThis work presents a newly developed formulation that holds great potential as a therapeutic approach for burn treatment. It is based on the sustained delivery of an antimicrobial peptide – LLKKK18 – from conjugates with dextrin, after degradation of dextrin backbone upon exposure to wound α-amylases. Conjugates were further embedded in Carbopol®, a commercially available hydrogel, suitable for topical administration and that provides a moist environment to the wound. Overall, we obtained an efficient, safe and non-expensive formulation that improves burn wound healing, maintains a moist environment within the wound, is easy to apply-and-remove, and has potential to prevent infection due to the presence of an antimicrobial peptide. Importantly, this is the first time the wound healing ability of LLKKK18 is demonstrated and that its main mechanisms of action are identified.

Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents.

BackgroundEvery year thousands of people are victims of burns, mainly scald burns. Many of these victims have small size wounds and superficial partial thickness and do not seek specialized medical care. As in Brazil Casearia sylvestris Sw., popularly known as guaçatonga is widely used for its analgesic, antiseptic and anti-inflammatory activities, this study sought to evaluate the effects of its hydroalcoholic extract in healing process of burns injuries.MethodsThe obtained extract was validated applying a thin layer chromatography and sophisticated validation method using Bothrops jararacussu snake venom that is necrotic and inflammatory, and by which guaçatonga extract was able to neutralize the irreversible neuromuscular blockade induced by the venom. After induction of the scald injury, the animals were treated daily with saline solution spray; spray containing extract; biofilm; or biofilm impregnated with extract.ResultsSignificant differences were observed between the four groups studied considering: extension of the healing area, neovascularization, fibroblast proliferation, and epithelialization.ConclusionThe anti-inflammatory and bactericidal effects of C. sylvestris Sw. suggests a potential therapeutic benefit in the treatment of inflammatory conditions in second-degree scald burn injuries, as well as, counteracting against the in vitro paralysis induced by B. jararacussu venom.

Proses Penyembuhan Luka Bakar pada Mencit Putih Jantan Menggunakan Membran Pembalut dari Pati Bengkuang (Pachyrrhizus erosus (L) Urban)

Study of the effect of varying the concentration of the absorption propilenglicol membrane wound dressing yam starch in the healing process of burns on white mice. The concentration of propilenglicol are used 20%, 30%, 40% of the total analyzed poliblen. Parameter of analysis are organoleptic, membrane thickness, pH,absorption test , antibacterial activity and pharmacological activity. The reasults show that concentrations propilenglicol effect was not significantly different in membrane thickness formula 1 and formula 2, but significantly different from the formula 3, the absorption test formula 3 have the better absorption of the formula 1 and formula 2. in the healing process of burn, healing percentages were not significantly different in each formula, but significantly different from controls. Keywords : Yam bean, plasticizer, membrane wound dressing, burns

PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus.&#x0D; Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test.&#x0D; Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats.&#x0D; Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

Effects of red laser, infrared, photodynamic therapy, and green LED on the healing process of third-degree burns: clinical and histological study in rats.

The aim of this study was to evaluate the effects of red laser, infrared, photodynamic therapy, and green light-emitting diode (LED) on the healing process of skin burns through clinical and histopathologic analysis in rats. For this, 100 animals were randomly divided into five groups: G1-untreated control (CTR), G2-red laser (LVER), G3-infrared (LINF), G4-photodynamic therapy (PDT), and G5-green LED. Burn was induced on the dorsum of the rat and the treatment of the experimental groups was red light (10 J/cm(2), 10 s, 40 mW, and λ660 nm), infrared (10 J/cm(2), 10 s, 40 mW, and λ780 nm), green LED irradiation (60 J/cm(2), 10 s, λ520, and 550 nm), and photodynamic therapy (10 J/cm(2), 40 mW, and λ660 nm), the latter combined with methylene blue photosensitizer at concentration 0.5 μg/mL. Applications were performed daily until day prior to sacrifice of the animal at 3, 7, 14, and 21 days with intraperitoneal anesthetic overdose. The specimens collected were clinically examined and soon after processed and stained with hematoxylin-eosin and Picrosirius for analysis under light and polarized light microscopy, respectively. Animals treated with LVER, LINF, PDT (p < 0.001), and LED (p < 0.05) stimulated production and maturation of collagen, and increased the consumption of food and water compared to the CTR (p < 0.001). Laser λ660 nm and λ780 nm showed the largest wound reductions in all groups (p = 0.001). In conclusion, red laser, infrared, photodynamic therapy, and green LED favored the healing process of third-degree burns in rats.

Effect of Laser Photostimulation on the Healing of Third-Degree Burn wounds in Rats

Background: Burns are one of the most common injuries in the world, most importantly during the wars, and the patients usually experience high levels of pain. Complications include both physical and psychological aspects as well as aesthetic concerns. Management comprises special strategies via specific treatment protocols. Treatment stages include: rescue and control of breathing rhythm, hemorrhage management, fracture closure, particle removal, infection control and control of body temperature. It has been suggested that laser therapy with low level energy could have a positive effect on wound healing process. Objectives: The aim of this study was to examine the effect of different wavelengths of Ga-As laser with blue (405 nm) and green (532 nm) wavelengths on the healing of third-degree burn wounds in Wistar rats. Materials and Methods: In this study, 36 male Wistar rats (8-weekold, weighting 250-300 g) were divided into three groups of equal numbers. All animals were anesthetized by intraperitoneal injections. Dorsal hairs were shaved and third-degree skin burns were created on the back of the animals by using a copper stamp. The stamp was warmed up first to 95˚C via a heater. Afterwards, the animals were randomly divided into the blue and green laser and control groups. The length and width of each wound were measured by a caliper and the area of the lesion was calculated on the first, third, seventh, tenth, fourteenth, seventeenth, and twenty-first days of the study. Six samples were taken from each group for pathobiological analysis. Results: A mild anti-inflammatory effect of blue laser was observed during acute phase, which could possibly accelerate wound healing during the first week of repair. Results of our study showed that using low-level laser therapy (LLLT) with green and blue wavelengths may accelerate the wound healing process as compared with the control group. Conclusions: The laser groups have a more rapid rate of wound contraction than the control group during the healing process of burns; however, the effect of LLLT on burn wound healing was neither statistically nor histologically significant. More studies should be performed to investigate other treatment protocols and prescriptions of soft laser to clarify its role in the treatment of wounds and burn injuries.

Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review

Burn wound healing is a complicated process including inflammation, re-epithelialization, granulation, neovascularization and wound contraction. Several biochemicals are involved in burn healing process including antioxidants, cytokines and liver and kidney damage biomarkers. Although several preparations are available for the management of burn wound, there is still a necessity of researching for efficacious medicine. The aim of the present study was to evaluate herbal preparations and their phytochemical constituents for burn wound management. For this purpose, electronic databases including Pubmed, Scirus, Scopus and Cochrane library were searched from 1966 to July 2013 for in vitro, in vivo or clinical studies which examined the effect of any herbal preparation on different types of burn wound. Only 3 human studies were found to include in this review. In contrast, there were 62 in vivo and in vitro studies that show the need for more clinical trials to prove the plant's potential to cure burn wound. Among single herbal preparations, Allium sativum, Aloe vera, Centella asiatica and Hippophae rhamnoides showed the best burn wound healing activity. Flavonoids, alkaloids, saponins and phenolic compounds were active constituents present in different herbs facilitating wound closure. Glycosides including madecassoside and asiaticoside and proteolytic enzymes were among the main active components. Phytochemicals represented positive activity at different stages of burn wound healing process by various mechanisms including antimicrobial, anti-inflammatory, antioxidant, collagen synthesis stimulation, cell proliferative and angiogenic effect. Overall, several herbal medicaments have shown marked activity in the management of wounds-especially burn wounds-and therefore can be considered as an alternative source of treatment. Furthermore, various natural compounds with verified burn-induced wound healing potential can be assumed as future natural drugs.

&lt;b&gt; Comparative study of Low-level laser therapy and microcurrent on the healing of skin burns in rats&lt;/b&gt;

This study investigated and compared the effects of low-level laser therapy (LLLT) and microcurrent in the burn healing process in Wistar rats. We conducted a randomized controlled study with 30 rats divided into 3 groups (n = 10); control group (CG), laser group (LG) and microcurrent group (MG). After thermal damage, 10 applications of 660 nm diode laser were performed in GL and 10 applications of 60 Hz microcurrent (160 μA) in MG. The semi-quantitative histological analysis was done using scores (0-3), in sections stained by hematoxylin and eosin and Masson's trichrome. The results indicated a significant improvement in the fibroblasts proliferation, collagen fibers deposition, neoangiogenesis, and cutaneous appendages regeneration in MG and LG. When microcurrent and LLLT were compared, no difference was detected, except the regeneration and formation of new cutaneous appendages, observed in MG. Despite the similar effects, GM showed faster tissue repair with the formation of skin appendages.

Healing Process Of Burns Using Ambarella Leaf (Spondias dulcis F.) and Vaselin in Rats (Rattus norvegicus)

The aims of this research was to find out the healing process of second degree burn in rats (Rattus norvegicus) using ambarella leaf (Spondias dulcis F.) and vaselin on histopathologically. The research consisted of 4 treatment groups and 3 rats each, P1(double distilled water), P2 (ambarella leaf + double distilled water), P3 (vaselin), and P4 (ambarella leaf + vaselin). Treatment was done twice a day for 21 days. The research parameters are inflammatory reactions and distribution of collagen tissue. Data were analyzed descriptively. The results showed that on day 7, P1 and P3 many infiltration of inflammatory cells, hemorrhage and edema, on P2 and P4 here more infiltration of inflammatory cells, hemorrhage and edema. On the day 14th of many inflammatory cell infiltration, collagen tissue diffuse very thin on were described P1 and P3, and visible presence of new blood vessels was found on P4. On day 21th infiltration of inflammatory cells still present on (P1), there were a lot of new blood vessels on P2, there has been a process of epithelialization (P3), epidermis formed and collagen tissue spread very densely on P4. Based on the results of this study it can be concluded that administration of ambarella leaf and vaselin can speed up the healing process of burns in rats compared with other treatments.Key words: ambarella leaf, burn, vaselin

Centella asiaticain Dermatology: An Overview

Centella asiatica is a medicinal plant that was already used as a 'panacea' 3000 years ago. The active compounds include pentacyclic triterpenes, mainly asiaticoside, madecasosside, asiatic acid and madecassic acid. We have conducted an overview to summarize current knowledge on the results of scientific in vitro and in vivo experiments focused on the improvement of the healing process of small wounds, hypertrophic scars and burns by C. asiatica. In this paper, we discuss the data on constituents, recommended preparations and the potential side effects of C. asiatica.

Microparticles of Aloe vera/vitamin E/chitosan: Microscopic, a nuclear imaging and an in vivo test analysis for burn treatment

The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes. In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan. In order to observe the morphological properties, Raman and atomic force microscopy evaluation were performed. The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process. The results of AFM analysis show the formation and the adherence property of the microparticles. Raman analyses show the distribution of each component in the microparticle. The nuclear method used shows that the biodistribution of the microparticles remained in the skin. The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.

Digital photogrammetry and histomorphometric assessment of the effect of non-coherent light (light-emitting diode) therapy (λ640 ± 20 nm) on the repair of third-degree burns in rats

Recent studies have demonstrated the efficacy of coherent light therapy from the red region of the electromagnetic spectrum on the tissue-healing process. This study analysed the effect of non-coherent light therapy (light-emitting diode-LED) with or without silver sulfadiazine (sulpha) on the healing process of third-degree burns. In this study, 72 rats with third-degree burns were randomly divided into six groups (n = 12): Gr1 (control), Gr2 (non-contact LED), Gr3 (contact LED), Gr4 (sulfadiazine), Gr5 (sulfadiazine + non-contact LED) and Gr6 (sulfadiazine + contact LED). The groups treated with LED therapy received treatment every 48 h (λ = 640 ± 20 nm, 110 mW, 16 J/cm(2); 41 s with contact and 680 s without contact). The digital photometric and histomorphometric analyses were conducted after the burn occurred. The combination of sulpha and LED (contact or non-contact) improved the healing of burn wounds. These results demonstrate that the combination of silver sulfadiazine with LED therapy (λ = 640 ± 20 nm, 4 J/cm(2), without contact) improves healing of third-degree burn wounds, significantly reduces the lesion area and increases the granulation tissue, increases the number of fibroblasts, promotes collagen synthesis and prevents burn infections by accelerating recovery.