Guajava Research Articles

Synergistic effects of Psidium guajava and procaine penicillin on MRSA, MSSA and E. faecalis

Background: Antibiotic resistance is a significant public health challenge, exacerbated by resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA).Aim: This study investigates the synergistic antibacterial effects of Psidium guajava aqueous extract combined with procaine penicillin against MRSA, methicillin-sensitive S. aureus (MSSA) and Enterococcus faecalis.Setting: In-vitro study conducted under controlled laboratory settings at the Mangosuthu University of Technology, Department of Biomedical Sciences, Kwa-Zulu Natal, South Africa.Methods: Psidium guajava leaves were collected and prepared according to the German Homeopathic Pharmacopoeia, while procaine penicillin was diluted for testing. The combination treatment was evaluated using the Kirby–Bauer Antimicrobial Sensitivity Test and Minimum Inhibitory Concentration (MIC) assays.Results: Results showed that the combination of P. guajava extract and procaine penicillin significantly enhanced the antibacterial activity against MRSA, as evidenced by larger zones of inhibition and lower MIC values compared to P. guajava extract and procaine penicillin on their own. However, no significant difference was observed against MSSA and E. faecalis.Conclusion: The findings suggest that P. guajava extract could potentiate the efficacy of procaine penicillin against MRSA, providing a potential alternative strategy to combat antibiotic-resistant bacteria.Contribution: The study revealed that P. guajava aqueous extract enhances the antibacterial efficacy of procaine penicillin specifically against MRSA, suggesting a potential alternative treatment strategy for antibiotic-resistant infections, validated through robust methods and highlighting the need for further research to understand the mechanisms and clinical applications.

Anxiolytic and Antianhedonic-like Effects of Psidium guajava Leaf in Alcohol-Withdrawn Mice.

Alcohol withdrawal syndrome manifests through a range of symptoms, including anxiety and anhedonia, significantly affecting the quality of life of those affected. This study investigates the potential therapeutic effects of the methanolic extract of Psidium guajava leaves (MPG) on anxiety and anhedonia in Swiss albino female mice undergoing alcohol withdrawal. Four groups of mice underwent alcohol withdrawal, with one group undergoing saline withdrawal as a control. On the test day, behavioral assessments were conducted to evaluate anxiety and anhedonia. Groups I and II received sodium carboxymethylcellulose, Group III received diazepam, and Groups IV and V received varying oral doses of MPG. The results indicate significant anti-anhedonic and anxiolytic effects of MPG. These effects were observed through changes in parameters measured in the Open Field test, Elevated Plus Maze test, Marble Burying test, and Sucrose Preference test. Mice treated with MPG displayed reduced anxiety-like behaviors and increased sucrose preference compared to untreated mice undergoing alcohol withdrawal. These findings suggest that Psidium guajava leaf extract may have therapeutic potential in alleviating anxiety and anhedonia associated with alcohol withdrawal. The observed effects indicate that MPG could serve as a promising adjunct therapy for managing alcohol withdrawal symptoms, thereby enhancing the overall well-being of individuals undergoing alcohol cessation.

Effect of pre-harvest application of potassium nitrate, naphthalene acetic acid and ethephon on ripening and shelf-life of guava cv. Allahabad Safeda

The current study, effect of pre-harvest application of potassium nitrate (KNO3), naphthalene acetic acid (NAA) and ethephon on ripening and shelf-life of guava (Psidium guajava L.) cv. Allahabad Safeda was undertaken at the Fruit Research Farm, Dr B R S Agri at Sri Muktsar Sahib, Punjab. The experiment was carried out on an eight-year-old guava tree of the Allahabad Safeda. The trees were sprayed with various doses of chemicals, KNO3 (1.0, 1.5, 2.0%), NAA (200, 300, 400 ppm) and ethephon (200, 400, 600 ppm) during the third week of November. The fruits were harvested at the hard ripe stage on 19 December, placed in Corrugated fibre box (CFB) of 4 kg capacity and stored at ambient temperature. In ambient storage, guava fruits were remain acceptable for up to 6 days of storage without any adverse effect on fruit quality. KNO3 (1.5 and 2.0%), NAA (300 ppm) and ethephon (200 ppm) recorded a reduction in physiological loss of weight and spoilage and KNO3 (2.0%) maintaining fruit firmness (7.64 kg/cm2), palatability rating (8.00) throughout the storage period. However, the highest storage life and good-quality fruits was recorded with the application of KNO3 (1.5, 2%) during ambient storage. At lower concentrations, ethephon also helps to enhance the ripening of fruits, contrast to this fruits treated with NAA delayed the fruit senescense.

Evaluation of Synergistic Antioxidant Activity of Psidium Guajava and Raw Honey- An Invitro study

Evaluation of Synergistic Antioxidant Activity of Psidium Guajava and Raw Honey- An Invitro study

Effect of Red Guava (Psidium guajava L.) Juice Concentration on Product Quality and Antioxidant Activity of Red Guava Synbiotic Yoghurt

Red guava juice has very strong antioxidant activity and has the potential to be processed into synbiotic functional beverage products that have health benefits. This study examined the effect of red guava juice concentration on product quality and antioxidant activity of red guava synbiotic yoghurt. Product quality tested included microbiological quality (total LAB) and chemical quality (pH value and Total Tritable Acid (TTA )value). The concentrations of red guava juice used were 0%, 10%, 20%, 30%, and 40%. Fermentation was carried out for 6 hours at 37⁰C by Lactic Acid Bacteria (LAB) from powdered mixed culture. Then organoleptic testing using the hedonic test, total LAB testing using the Total Plate Count (TPC) method, pH value testing using a pH meter, TTA value testing using the acid-base titration method, and antioxidant activity testing using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. Test data were analyzed using SPSS software to determine the effect of red guava juice concentration on test results. The concentration of red guava juice in red guava synbiotic yoghurt affects the pH value, TTA value, and IC50 value as the result of antioxidant activity testing. Red guava juice concentration did not affect total LAB testing. Organoleptic assessment of red guava symbiotic yoghurt is in the range of like to like very much. The best formulation was red guava synbiotic yoghurt with 30% red guava juice concentration, which had strong antioxidant activity with an IC50 value of 59.637 ppm, pH value of 3.94, TTA value of 1.98%, and total LAB as much as 1.81x10⁸ colonies/g, so it has met the quality requirements of yoghurt according to Indonesian National Standard SNI 2981: 2009.

Effects of Plant Extracts on Growth Promotion, Antioxidant Enzymes, and Secondary Metabolites in Rice (Oryza sativa) Plants

Plant extracts are widely used in sustainable agriculture practices to enhance crop production and reduce chemical usage in agriculture. This study employed several extraction solutions of various plant extracts to synthesize planting and spraying strategies, assess the persistence efficacy of rice, and investigate the influence of selected water extracts on secondary chemicals at different rice planting stages. Among 17 water extracts that were evaluated on rice seeds, 7 were enhanced to align with the lengths of rice roots 50–70% and shoots 40–50%. The analysis of extraction, spraying, and planting experiments revealed that water extracts, soil application, and transplanting were the most efficient methods for stimulating rice growth, especially 0.1 and 0.5% concentrations. The efficacy of the extracts remained intact also after 14 days of treatment. This study showed that photosynthesis and antioxidant activities may play crucial roles in plant growth. Rice growth stimulation has been linked to photosynthesis, flavonoid contents, and antioxidant enzymes, providing a balanced supply of nutrients for plant growth. Among all tested water extracts, Psidium guajava, Aloe vera, Allium sativum, and Medicago sativa extracts can be used to promote plant growth in organic farming.

Pulp colour and pigment composition have no genetic correlation with seed hardness in guava (Psidium guajava L.)

ABSTRACT Guava (Psidium guajava L.) is a ‘super fruit’, also known as the ‘apple of the tropics’. This study was conducted on five guava parents and their 42 F1s. They were studied for their pigment composition (lycopene, total anthocyanin, and total carotenoid content) and seed-based parameters (seed hardness, index, length, and width). Transgressive segregation was present for pigment composition, seed index, and seed hardness. For lycopene and total carotenoids content, F1s had maximum values. However, for total anthocyanins content, only guava parent BG had a considerably higher value (8.881 mg/100 g) and had a purple pulp colour. Most F1s belonged to the soft seed category (28 F1s out of 42) and intermediate seed index 1–1.5 g (24 F1s out of 42). The findings indicate that, in guava, pulp colour is not correlated with seed hardness. However, a significantly positive correlation between seed hardness and other seed-related parameters (namely seed index, seed length, and seed width) indicates that a bigger seed size is associated with seed hardness.

Variation of vitamin C content and antioxidant capacities during the post-harvest storage of fresh fruits under different temperatures

During post-harvest storage, vitamins and health promoting compounds of fresh fruits are prone to change. Variation in ascorbic acid (ASC), total vitamin C (VitC), total phenolic (TPC) and total flavonoid (TFC) and antioxidant capacities (ACs) were studied during storage of fresh fruits (31 species) for 14 days at three different temperatures. During storage, ASC (1.6–90.0%) and VitC (0.1–71.6%) contents decreased significantly in most of the fruits, irrespective to the storage temperature. Interestingly accumulation of ASC (1.0–245.0%) and VitC (0.5–102.8%) was also observed in some fruit species; Musa paradisiaca, Aegal marmelos, Citrus aurantifolia and Passiflora edulis. All the fruits with the highest rate of accumulation of ASC and VitC are climacteric fruits; Musa paradisiaca, Persea americana etc. In Psidium guajava, almost all the measured health promoting compounds were significantly lost during storage. In most of the under-utilized fruits, significant loss in TPC (Artocarpus heterophyllus (‘Wela’) at room temperature: 87.4%), TFC (Averrhoa carambola: 100.0%) and ACs could be observed. The highest percentage of losses (100.0%) in DPPH radical scavenging assay and Ferric reducing antioxidant potential were observed in Citrullus lantanus. According to the results of multivariate analysis of variance (MANOVA), there is a statistically significant effect (p < 0.0005) of fruit species, storage temperature and an interaction effect of those on changing rates of measured antioxidants. The reduction rates of measured antioxidants are significantly highest at room temperature, while which are significantly highest under freezing conditions compared to refrigeration. Refrigeration temperature is recommended for optimization of preservation of vitamin C and antioxidants during storage of fresh fruits.

Exploring zinc impact on rooting efficiency in guava (Psidium guajava) cuttings under shade net environment

Exploring zinc impact on rooting efficiency in guava (Psidium guajava) cuttings under shade net environment

Psidium Exotic and Native Species from Brazil Abolish Depression-like Behavior and Oxidative Stress induced by Corticosterone in Mice.

Depression is a highly prevalent neuropsychiatric disorder worldwide. One currently accepted hypothesis of this pathogenesis is the hypothalamic-pituitary-adrenal axis dysfunction, which involves oxidative stress and brain damage. Therefore, antioxidants, such as phenolic compounds, could be used in depression. In this study, we investigated the antidepressant-like and antioxidant effects of an aqueous extract of the leaves of three species of the genus Psidium, Myrtaceae family, in mice. The exotic Psidium guajava L.and the natives Psidium guineense Sw. and Psidium cattleianum Sabine (10, 1, and 0.1 mg/kg, respectively) and fluoxetine (10 mg/kg) were administered orally (p. o.) once daily for 21 days, with or without corticosterone (20 mg/kg). After behavioral assessments (tail suspension, splash, and open-field tests), the hippocampus, prefrontal cortex, liver, kidneys, and plasma were examined to determine the oxidative stress status. The three extracts and fluoxetine treatment decreased the immobility time and counteracted the oxidative stress induced by corticosterone administration. The phenolic compounds identified as major components of the extracts, quercetin in P. guajava and P. guineense and o-coumaric acid in P. cattleianum, may be involved in the biological activities. Therefore, the aqueous leaf extracts of P. guajava, P. cattleianum, and P. guineense could be potential antidepressants helpful in treating depression and other diseases with elevated nitro-oxidative stress.

First report of Neopestalotiopsis clavispora Causing guava scab in China.

Psidium guajava L. is widely cultivated in southern China. In May 2021, guava scab on cv. Zhenzhu was observed in Zhanjiang (21.18° N, 110.21° E), Guangdong province, China. Guavascab was corky with ovoid or round lesions on the surfaces of green fruits. Gradually the lesions sunk. Disease incidence was estimated as 85% in 500 investigated plants in about 50 ha. Twenty diseased fruits were collected from twenty trees in the field. From each fruit the margin of the diseased tissues was cut into 2 mm × 2 mm pieces; surface disinfected with 75% ethanol and 2% sodium hypochlorite for 30 and 60 s, successively; and rinsed thrice with sterile water. The tissues were plated onto potato dextrose agar (PDA) medium and incubated at 28 ℃. Thirty-four isolates were obtained. Single-spore isolation method (Liu et al. 2021) was used to recover pure cultures of three isolates (PGNC-1, PGNC-2, and PGNC-3) . The colonies were initially white with cottony aerial mycelium at 7 days on PDA. Then, these colonies form black acervular conidiomata at 10 days. Conidia were clavate to fusiform, four-septate, straight or slightly curved, and measured 15.8 to 21.2 µm × 4.5 to 6.5 µm (n = 40). The three median cells were versicolored, whereas the basal and apical cells were hyaline. Conidia had a single basal appendage (4.5 to 5.5 µm long; n = 40) and three apical appendages (19.2 to 24.5 µm long; n = 40). The morphological characteristics of the isolates were consistent with the description of Neopestalotiopsisclavispora (Maharachchikumbura et al. 2012). Molecular identification was performed using PCR method with MightyAmp DNA Polymerase (Takara-Bio, Dalian, China) (Lu et al. 2012). Sequences were generated from the isolates using primers for the rDNA ITS (ITS1/ITS4), TEF1-α (EF1-728F/EF1-986R), and β-tubulin (T1/βt2b) loci (Maharachchikumbura et al. 2012). The sequences of the isolates were submitted to GenBank (ITS, OQ996557 to OQ996559; TEF, OR101037 to OR101039; β-tubulin, OR100971 to OR100973). The sequences of the isolates were 100% identical to the type strain MFLUCC12-0281 (accession nos. JX398979, JX399014, and JX399045) through BLAST analysis. The isolates clustered with N. clavispora (MFLUCC12-0280 and MFLUCC12-0281). N. clavispora and Pestalotiopsis clavispora are synonyms. The pathogenicity was tested in vivo. Plants (cv. Zhenzhu) were grown ( 3 years old) in a quarantine orchard at 25℃ to 32℃ with 60 to 80% relative humidity in May 2022. Disease-free green fruits were inoculated. Sterile cotton balls were immersed in the spore suspension (1 × 105 per mL) and sterile distilled water (control) for about 15 s before they were fixed on the wounded fruits with transparent tape. Five fruits on one plant per isolate were inoculated. Five fruits on one plant severed as control. The test was performed thrice. Disease symptoms were found on the inoculated fruits after 20 days, whereas the controls remained healthy. The pathogen was re-isolated from infected fruits and was phenotypically identical to the original isolates thus fulfilling Koch's postulates. Neopestalotiopsis or Pestalotiopsis spp. were reported to be the causal agents of guava scab in Colombia and in Hawaii (Keith et al. 2006; Solarte et al. 2018). N. clavispora has been reported to cause disease in a broad range of hosts (Ge et al. 2009; Chen et al. 2018), but not in guava. This is the first report of N. clavispora causing guava scab in China. There would be no harvest if this disease is left unmanaged.

An economic, and environmentally benign Psidium guajava L. leaves catalyst for biodiesel production at room temperature: Process optimization, and life cycle cost analysis

Biodiesel has emerged as a popular alternative to conventional fossil fuel in transportation sector and thus producing low-cost biodiesel is a topic of interest for a large community of researchers. This study reports the preparation of an efficient and robust heterogeneous catalyst from dried Psidium guajava L. (Guava) leaves and its application towards generation of biodiesel from soybean oil at room temperature. The biomass material was calcinated at variable temperature and the efficiency of each of the catalyst prepared was studied. The catalyst generated as well as the product biodiesel was thoroughly characterized using sophisticated analytical techniques. The catalyst was found to have mesoporous structure with active basic sites which contributed towards accelerating the transesterification process. Detailed characterization unveiled the occurrence of Ca as oxide and carbonate, in addition to a certain quantity of K in oxide and carbonate form, which contribute to the catalyst’s activity. Maximum biodiesel yield of 95.8 % was observed for reaction performed under optimum experimental conditions i.e., 1:17 oil-to-methanol ratio, 6 wt% loading of GL-850 (catalyst prepared by calcination at 850°C), and 15 % n-Hexane loading for 3 h at room temperature. GL-850 demonstrated exceptional recyclability for up to the fifth cycle exhibiting a mere 3.7 % decline in yield. The calculated expense for 1 kg catalyst was found to be $0.478, whereas the production cost of 1 kg biodiesel was found to be merely $1.120, indicating a strong economic feasibility of the process.

Effect of Leaf and Seed Powder Based Edible Coating with Beeswax and Clove Essential Oil on the Shelf Life of Guava (Psidium guajava) cv. Allahabad Safeda

The guava is thought to be India's most significant commercial fruit crop. Due to their high respiration rate, vulnerability to different pathogens, and mechanical damage that can quickly deteriorate the quality and concised its shelf life. Nonetheless, the purpose of the experiment was to investigate how guava's edible coatings affected its postharvest quality and shelf life. Guava fruits were treated by seven treatments of different concentrations of beeswax, guava leaf powder, neem leaf powder, tamarind seed powder, and clove oil with different combinations were used as edible coating were studied in a Factorial Completely Randomized Design (CRD) with three replications. All seven treatments were used to examine the impact of edible coating materials on the physical and chemical properties. Under ambient conditions, the variations in physiological and chemical parameters were seen. The findings of present study showed maximum fruit weight (120.29 g), highest vitamin C (163.19 mg/100g), maximum acidity (0.49%) and antioxidant activity (35.43%) obtained from T4 [Neem leaves powder (2%) + Beeswax (1%)+ clove oil (1%)] compared with untreated fruits. The result suggested that the applications of [Neem leaves powder (2%) + Beeswax (1%) + clove oil (1%)] as an edible coating material in guava fruit is more effective than other treatments to improve the shelf life of Guava.

Guaijaverin And Epigallocatechin Gallate Exerts Antiinflammatory And Antiallergenic Effects Through Interleukin-12 Production.

Our aim in the current study was to determine the in vitro and invivo synergistic antiinflammatory and antiallergic effect associated with the IL-12 production of guaijaverin and epigallocatechin gallate (EGCG) complex (GEC) and ILS-F-2301 (2:8 extract of Psidium guajava and Camellia sinensis). Compared to EGCG alone, GEC showed synergistic inhibition of nitric oxide (NO), inducible NO synthase, and cyclooxygenase-2 by 3.8, 5.1, and 4.1%, respectively. The downregulation of interleukin-12 (IL-12) by 2,4-dinitrophenyl-human serum albumin conjugate/DNP-immunoglobulin E or ovalbumin (OVA) was synergistically increased by GEC by about 7.5% or 5.4% compared to EGCG alone. The level of downregulation of IL-12 in plasma increased by 100 mg/kg with ILS-F-2301 (28.7%) when compared to the OVA/Alu-treated group. Also, GEC synergistically increased by GEC by about 7.5% or 5.4% compared to EGCG alone. The level of down and cyclooxygenase C synergistically inhibited p-Akt, PI3K, mTOR, p-STAT6, and GATA3 by 4.9%, 4.1%, 19.2%, 23.8%, and 35.3%, respectively, while increasing the expressions of p-STAT1 and T-bet (showing 53.3% and 9.4% activation) when compared to EGCG alone. In an allergenic rhinitis mouse model, 100 mg/kg of ILS-F-2301 was shown to inhibit p-Akt, PI3K, mTOR, p-c-Jun N-terminal kinase (p-JNK), p-extracellular signal-regulated kinase (p-ERK), and p-p38 by 23.3%, 43.8%, 17.2%, 32.2%, 29.1%, and 41.8% when compared to the OVA/Alu-sensitized group. Taken together, our findings suggest that ILS-F-2301 may have potential as a functional food for alleviating antiallergic rhinitis.

Phytochemical Screening And Antimcrobial Efficacy of Leaf Extract Of Guava (Psidium guajava) On Selected Bacteria

Guava leaf extract has analgesic, anti-inflammatory, antimicrobial, hepato protective and antioxidant activities. The high resistance of bacteria to modern day drugs has led researchers in the pharmaceutical industry to look towards plant-based extracts for solutions. Hence, this studywas aimed at evaluating the phytochemical and antibacterial efficacy of leaf extracts of Psidium guajava (guava) on some selected bacteria. Pure isolates of bacteria gotten from urine of patients in Charis Rhema Research and Diagnostic Laboratory were used in the study. Extracts from the leaves of Psidium guajava were tested for presence of phytochemicals. Separation of active components of the extracts was one using Chromatographic technique. The antibacterial activityof Psidium guajava leaves was determined using agar diffusion bioassay. Phenols were present at all concentrations (100, 80 and 60mg). The study discovered a significant relationship between extract concentration and the zones of inhibition at 60% N-hexane/40% methanol for Klebsiellasp., and 0.0% N-hexane/100% methanol for S. aureus. Flavonoids, tannins, saponins and phenols were the active compounds in guava that helped in the inhibition of growth on the sampled isolates. Methanol and N-hexane extracts of guava can be used as potent medicines for inhibitingthe growth of Klebsiella and Staphylococcus aureus.

Evaluation of PGRrsquo;s, girth of hard wood stem cuttings and rooting media for rapid multiplication of guava (Psidium guajava L.) cv. Allahabad Safeda

Evaluation of PGRrsquo;s, girth of hard wood stem cuttings and rooting media for rapid multiplication of guava (Psidium guajava L.) cv. Allahabad Safeda

Enhancing post-harvest shelf life of guava (Psidium guajava L.) using herbal coating enriched with Allium cepa green leaves

Enhancing post-harvest shelf life of guava (Psidium guajava L.) using herbal coating enriched with Allium cepa green leaves

Evaluating the effect of plant growth regulators on qualitative characteristics of guava (Psidium guajava L.)

Evaluating the effect of plant growth regulators on qualitative characteristics of guava (Psidium guajava L.)

Guaijaverin and Epigallocatechin Gallate Complex Modulate Th1 and Th2 Cytokine-Mediated Allergic Responses Through STAT1/T-bet and STAT6/GATA3 Pathways.

We aimed to determine the in vitro and invivo synergistic antiallergic effect of guaijaverin and epigallocatechin gallate (EGCG) complex (GEC), and the antiallergic rhinitis (AR) properties of guaijaverin-rich Psidium guajava and EGCG-rich Camellia sinensis (ILS-F-2301). GEC showed synergistic inhibition of β-hexosaminidase by 4.20% and interleukin (IL)-4, -5, and -13 by 4.08%, 0.67%, and 4.71%, respectively, while increasing interferon (IFN)-γ by 12.43%, compared with EGCG only. In addition, 50 μg/mL of ILS-F-2301 inhibited β-hexosaminidase release, and inhibited IL-4, -5, and -13 by 61.54%, 58.79%, and 59.25%, respectively, while increasing IFN-γ (showing 133.14% activation). Moreover, 50 μg/mL of ILS-F-2301 suppressed p-STAT6 and GATA3, while p-STAT1 and T-bet increased, and 0.039 μg/mL of guaijaverin or 5.275 μg/mL of EGCG modulated T helper (Th)1- and Th2-related proteins. These data suggested that guaijaverin and EGCG in ILS-F-2301 was the main active compound involved in Th1/Th2 modulation. In the AR mouse model, the administration of ILS-F-2301 inhibited ovalbumin (OVA)-specific IgE, histamine in serum; it also inhibited IL-4 and -5 by 28.23% and 47.15%, respectively, while increasing IFN-γ (showing 37.11% activation), compared with OVA/Alu-treated mice. Taken together, our findings suggest that ILS-F-2301 is a functional food for alleviating anti-AR.

Psidium guajav-mediated zinc oxide nanoparticles as a multifunctional, microbicidal, antioxidant and antiproliferative agent against destructive pathogens.

Bio-inspired zinc oxide nanoparticles are gaining immense interest due to their safety, low cost, biocompatibility, and broad biological properties. In recent years, much research has been focused on plant-based nanoparticles, mainly for their eco-friendly, facile, and non-toxic character. Hence, the current study emphasized a bottom-up synthesis of zinc oxide nanoparticles (ZnO NPs) from Psidium guajava aqueous leaf extract and evaluation of its biological properties. The structural characteristic features of biosynthesized ZnO NPs were confirmed using various analytical methods, such as UV-Vis spectroscopy, X-ray diffraction (XRD), energy-dispersive X-ray analysis (EDX), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), Scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HR-TEM). The synthesized ZnO NPs exhibited ahydrodynamic shape with an average particle size of 11.6-80.2nm. A significant antimicrobial efficiency with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 40 and 27µg/ml for Enterococcus faecalis, followed by 30 and 40µg/ml for Staphylococcus aureus, 20 and 30µg/ml for Staphylococcus mutans, 30µg/ml for Candida albicans was observed by ZnO NPs. Additionally, they showed significant breakdown of biofilms of Streptococcus mutans and Candida albicans indicating their future value in drug-resistance research. Furthermore, an excellent dose-dependent activity of antioxidant property was noticed with an IC50 of 9.89µg/ml. The antiproliferative potential of the ZnO NPs was indicated by the viability of MDA MB 231 cells, which showed adrastic decrease in response to increased concentrations of biosynthesized ZnO NPs. Thus, the present results open up vistas to explore their pharmaceutical potential for the development of targeted anticancer drugs in the future.