Phytochemical Screening And Antimcrobial Efficacy of Leaf Extract Of Guava (Psidium guajava) On Selected Bacteria

Abstract

Guava leaf extract has analgesic, anti-inflammatory, antimicrobial, hepato protective and antioxidant activities. The high resistance of bacteria to modern day drugs has led researchers in the pharmaceutical industry to look towards plant-based extracts for solutions. Hence, this studywas aimed at evaluating the phytochemical and antibacterial efficacy of leaf extracts of Psidium guajava (guava) on some selected bacteria. Pure isolates of bacteria gotten from urine of patients in Charis Rhema Research and Diagnostic Laboratory were used in the study. Extracts from the leaves of Psidium guajava were tested for presence of phytochemicals. Separation of active components of the extracts was one using Chromatographic technique. The antibacterial activityof Psidium guajava leaves was determined using agar diffusion bioassay. Phenols were present at all concentrations (100, 80 and 60mg). The study discovered a significant relationship between extract concentration and the zones of inhibition at 60% N-hexane/40% methanol for Klebsiellasp., and 0.0% N-hexane/100% methanol for S. aureus. Flavonoids, tannins, saponins and phenols were the active compounds in guava that helped in the inhibition of growth on the sampled isolates. Methanol and N-hexane extracts of guava can be used as potent medicines for inhibitingthe growth of Klebsiella and Staphylococcus aureus.