Abstract
Among the various inorganic nanoparticles, silver nanoparticles have received substantial attention in the field of antimicrobial research. For safe and biocompatible use of silver nanoparticles in antimicrobial research, the different biogenic routes are developed to synthesize silver nanoparticles that do not use toxic chemicals. Among those, to synthesize silver nanoparticles, the use of plant part extract becomes an emerging field because plant part acts as reducing as well as capping agent. For large-scale production of antibacterial silver nanoparticles using plant part, the synthesis route should be very simple, rapid, cost-effective and environment friendly based on easy availability and non-toxic nature of plant, stability and antibacterial potential of biosynthesized nanoparticles. In the present study, we report a very simple, rapid, cost-effective and environment friendly route for green synthesis of silver nanoparticles using guava (Psidium guajava) leaf extract as reducing as well as capping agent. This plant has been opted for the present study for its known medicinal properties, and it is easily available in all seasons and everywhere. The biosynthesized silver nanoparticles are characterized by UV–Vis and TEM analysis. The average particle size is 40 nm in the range of 10–90 nm. The antibacterial activity of these nanoparticles against Pseudomonas aeruginosa MTCC 741 has been measured by disc diffusion method, agar cup assay and serial dilution turbidity measurement assay. The results show that green synthesized silver nanoparticles, using guava (Psidium guajava) leaf extract, have a potential to inhibit the growth of bacteria.
Highlights
Nanomaterials and nanostructures, usually ranging from 1 to 100 nm, based on the specific characteristics such as size, morphology and distribution exhibit their remarkable potential in the field of biology and medicine
For large-scale production of antibacterial silver nanoparticles using plant part, the synthesis route should be very simple, rapid, cost-effective and environment friendly based on easy availability and non-toxic nature of plant, stability and antibacterial potential of biosynthesized nanoparticles
We report a very simple, rapid, cost-effective and environment friendly route for green synthesis of silver nanoparticles using guava (Psidium guajava) leaf extract as reducing as well as capping agent
Summary
Nanomaterials and nanostructures, usually ranging from 1 to 100 nm, based on the specific characteristics such as size, morphology and distribution exhibit their remarkable potential in the field of biology and medicine. As per our knowledge, to investigate the antimicrobial properties only few number of available plants leaf have been used to synthesize Ag nanoparticles till This motivates us to search a very simple, rapid, cost-effective and environment friendly synthesis route of Ag nanoparticles, using leaf extract, for the investigation of its antimicrobial activities. In this present study, an attempt has been done to synthesize silver nanoparticles using guava (Psidium guajava) leaf extract from the green chemistry perspective and to investigate the antimicrobial activities of these biosynthesized silver nanoparticles on Pseudomonas aeruginosa MTCC as a model gram-negative bacteria This plant has been opted for the present study for its known medicinal properties, and it is available in all seasons and everywhere
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