Plant Growth Processes Research Articles

Genome-Wide Identification and Expression Analysis of the Cucumber FKBP Gene Family in Response to Abiotic and Biotic Stresses.

The FKBP (FK506-binding protein) gene family is an important member of the PPlase protease family and plays a vital role during the processes of plant growth and development. However, no studies of the FKBP gene family have been reported in cucumber. In this study, 19 FKBP genes were identified in cucumber, which were located on chromosomes 1, 3, 4, 6, and 7. Phylogenetic analysis divided the cucumber FKBP genes into three subgroups. The FKBP genes in the same subgroup exhibited similar structures and conserved motifs. The cis-acting elements analysis revealed that the promoters of cucumber FKBP genes contained hormone-, stress-, and development-related cis-acting elements. Synteny analysis of the FKBP genes among cucumber, Arabidopsis, and rice showed that 12 kinds of syntenic relationships were detected between cucumber and Arabidopsis FKBP genes, and 3 kinds of syntenic relationships were observed between cucumber and rice FKBP genes. The tissue-specific expression analysis showed that some FKBP genes were expressed in all tissues, while others were only highly expressed in part of the 10 types of tissues. The expression profile analysis of cucumber FKBP genes under 13 types of stresses showed that the CsaV3_1G007080 gene was differentially expressed under abiotic stresses (high temperature, NaCl, silicon, and photoperiod) and biotic stresses (downy mildew, green mottle mosaic virus, Fusarium wilt, phytophthora capsica, angular leaf spot, and root-knot nematode), which indicated that the CsaV3_1G007080 gene plays an important role in the growth and development of cucumber. The interaction protein analysis showed that most of the proteins in the FKBP gene family interacted with each other. The results of this study will lay the foundation for further research on the molecular biological functions of the cucumber FKBP gene family.

Genome-wide analysis of PIN genes in cultivated peanuts (Arachis hypogaea L.): identification, subcellular localization, evolution, and expression patterns

BackgroundAuxin is an important hormone in plants and the PIN-FORMED (PIN) genes are essential to auxin distribution in growth and developmental processes of plants. Peanut is an influential cash crop, but research into PIN genes in peanuts remains limited.ResultsIn this study, 16 PIN genes were identified in the genome of cultivated peanut, resolving into four subfamilies. All PIN genes were predicted to be located in the plasma membrane and a subcellular location experiment confirmed this prediction for eight of them. The gene structure, cis-elements in the promoter, and evolutionary relationships were elucidated, facilitating our understanding of peanut PINs and their evolution. In addition, the expression patterns of these PINs in various tissues were analyzed according to a previously published transcriptome dataset and qRT-PCR, which gave us a clear understanding of the temporal and spatial expression of PIN genes in different growth stages and different tissues. The expression trend of homologous genes was similar. AhPIN2A and AhPIN2B exhibited predominant expression in roots. AhPIN1A-1 and AhPIN1B-1 displayed significant upregulation following peg penetration, suggesting a potential close association with peanut pod development. Furthermore, we presented the gene network and gene ontology enrichment of these PINs. Notably, AhABCB19 exhibited a co-expression relationship with AhPIN1A and AhPIN1B-1, with all three genes displaying higher expression levels in peanut pegs and pods. These findings reinforce their potential role in peanut pod development.ConclusionsThis study details a comprehensive analysis of PIN genes in cultivated peanuts and lays the foundation for subsequent studies of peanut gene function and phenotype.

Molecular evolution and interaction of 14-3-3 proteins with H+-ATPases in plant abiotic stresses.

Environmental stresses severely affect plant growth and crop productivity. Regulated by 14-3-3 proteins (14-3-3s), H+-ATPases (AHAs) are important proton pumps that can induce diverse secondary transport via channels and co-transporters for the abiotic stress response of plants. Many studies demonstrated the roles of 14-3-3s and AHAs in coordinating the processes of plant growth, phytohormone signaling, and stress responses. However, the molecular evolution of 14-3-3s and AHAs has not been summarized in parallel with evolutionary insights across multiple plant species. Here, we comprehensively review the roles of 14-3-3s and AHAs in cell signaling to enhance plant responses to diverse environmental stresses. We analyzed the molecular evolution of key proteins and functional domains that are associated with 14-3-3s and AHAs in plant growth and hormone signaling. The results revealed evolution, duplication, contraction, and expansion of 14-3-3s and AHAs in green plants. We also discussed the stress-specific expression of those 14-3-3and AHA genes in a eudicotyledon (Arabidopsis thaliana), a monocotyledon (Hordeum vulgare), and a moss (Physcomitrium patens) under abiotic stresses. We propose that 14-3-3s and AHAs respond to abiotic stresses through many important targets and signaling components of phytohormones, which could be promising to improve plant tolerance to single or multiple environmental stresses.

Systematic analysis of the expansin gene family in Nelumbo reveals candidate seed development responsive members in lotus (Nelumbo nucifera)

Expansins (EXPs) are plant cell wall proteins that not only induce cell wall extension in a pH-dependent manner, but also regulate plant growth and development process, as well as stress tolerance. This study systematically analyzed the EXP gene family in Nelumbo, and 62 genes, including 29 and 33 members were identified in the N. nucifera and N. lutea genomes, respectively. These genes could be classified into four subfamilies, including α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA), and expansin-like B (EXLB), with 40, 11, 5, and 6 members, respectively. Physicochemical analysis, motif structure, and chromosomal distribution of these genes were evaluated. Evolutionary analysis within or between the two Nelumbo genomes revealed strong purifying selection among the EXP genes, with segmental duplication emerging as the primary driver of the EXP gene family expansion in lotus. Moreover, synteny analysis conducted between lotus and other species has revealed that EXP genes exhibit varying evolutionary patterns across different plant species. Subcellular localization assay demonstrated that NnEXP proteins were localized in the cell wall. NnEXP gene expression analysis revealed that most members were expressed in at least one lotus tissue, and qRT-PCR indicated that some genes were differently expressed in lotus seeds at different developmental stages. Furthermore, the transient overexpression of NnEXPA2 has been demonstrated to enhance cell expansion during the early development of lotus seed cotyledons. This study not only forms a basis for functional characterization of lotus EXP genes, but also provides useful genetic resources for future lotus breeding.

A First Principles Investigation of the Bulk and Electronic Properties of Doped Armchair Graphene Nanoribbon for Sensing Dimethyl Disulphide

Studies have revealed that sulphur compounds from dimethyl disulphide, an essential semiochemical, support a number of crucial plant growth processes, including protein synthesis, nitrogen metabolism, and enzyme activity. Dimethyl disulphide is a crucial fertiliser that aids in the growth and promotion of diverse plant species, according to numerous research. Sensing the presence or level of sulphur can fundamentally help in managing the inputs that are delivered in the field, leading to the success of environmentally friendly agricultural practises. The availability of this crucial voc affects the vigour and longevity of plant species. In this article, we use the density functional theory approach to analyse the adsorption behaviour of a dimethyl disulphide molecule on a graphene nanoribbon doped with boron. The analysis of the nanoribbon's bulk, electrical, and transport properties demonstrates its potential for the detection of this crucial volatile organic molecule.

Increasing mustard (Brassica juncea L.) yields through exposure sound and preventive pest management based on refugia plants

Abstract. In Indonesia, mustard plants will increase by 8.2% in 2021, but more than that is needed to meet the community's needs due to a growing population. Pests and fertilization problems hinder expected growth. The provision of plant fertilizers can be replaced through the application of sound. Exposure sounds to plants can increase plant growth by opening stomata, improving water absorption, and increasing plant nutrient uptake. This study aimed to examine the application of sound effects and refugia plants t890po increase the growth and yield of mustard greens (Brassica juncea L.). The study used a Time's Series Randomized Block Design with 3 replications. The first factor is sound applications (S) consisting of S0 = without sound, S1 = rock music (Frequency 21-14,000 Hz), and S2 = classical sound (Frequency 21-14,000 Hz). The second factor is the refugia plant (R) consisting of R0 = without refugia, R1 = Tagetes erecta L., R2 = Zinnia elegans L, and R3 = Ocimum bassilicum L. The application of classical sound frequency (20-14,000 Hz) affects the growth process of mustard plants through the increase in plant height (14.5%), leaf area (20.92%), wet weight (3.5%), dry weight (26.41%), total chlorophyll (11.62%) and plant N content (31.03%). Meanwhile, rock music frequency (20-14,000 Hz) can increase plant height (2.97%), leaf area (19.11%), and total stomata (9.6%). Refugia plants affect the process of plant protection through their ability to attract pests in the research area.

Ecological and photosynthetic characteristics of Carex schmidtii with respect to hydrological fluctuations

Hydrological fluctuations are key abiotic stresses that influence plant growth and photosynthetic processes of wetland plants. However, the response mechanism of the plant characteristics and photosynthesis of Carex schmidtii, with respect to hydrological fluctuations, are still unclear. Comparative studies of plant size parameters, biomass parameters, and photosynthesis parameters of C. schmidtii under the interactive influence of initial water depth (WD), water-level amplitude (WA), and duration time, were performed. The coupling relationship between any two factors of the plant characteristics of C. schmidtii was also examined. Generally, the results showed that the WD and WA treatments, and the duration time, significantly affected plant size, biomass, and photosynthesis of C. schmidtii. The exception was the effect by the WA on the biomass and the NPQ. Furthermore, the biomass and photosynthesis of C. schmidtii were significantly affected by the interactive effects by WD and WA. The plant size, biomass, and photosynthesis parameters of C. schmidtii first increased and then decreased over time. Larger values of plant size, biomass, and photosynthesis parameters (except for qL and NPQ) were found for the treatment with an initial water depth of 0 cm. As was the situation with WD, the largest values of plant height, chlorophyll content, Fv/Fm, and ΔFv/Fm’ were obtained for a water-level amplitude of 0 cm. Furthermore, the chlorophyll content was positively related to the plant size parameters, Fv/Fm, ΔFv/Fm’, and qL. Except for qL, ΔFv/Fm’ had a significant relationship with all plant factors. Lower water levels and smaller water level changes are more conducive to the growth and photosynthese of C. schmidtii. Plant size, biomass accumulation and photosynthesis of C. schmidtii collectively coped with hydrological fluctuations. The findings have improved understanding of the response of C. schmidtii to hydrological fluctuations. They have thereby provided invaluable information for the restoration, management and conservation of tussock wetlands.

Heavy Metals Can Affect Plant Morphology and Limit Plant Growth and Photosynthesis Processes

Soil heavy metal pollution caused by human activities has become one of the most critical environmental issues with a global concern. Phytoremediation is widely used due to its low cost and environmental friendliness. However, the impact of heavy metals on plant growth remains unclear. This study investigated the effects on the growth and photosynthetic activity of Picris divaricata Vant. under different cadmium concentrations using a hydroponics cultivation system. The results showed that the growth and photosynthetic processes of P. divaricata exhibited a phenomenon of promotion in low Cd concentrations and inhibition in high Cd concentrations. Under a low to medium Cd concentration (≤25 μM), there was no Cd toxicity in terms of plant growth, but high concentrations of Cd inhibited plant growth. The Fe content of leaves gradually increased as the Cd concentration increased; it reached 201.8 mg kg−1 in 75 μM Cd. However, there was no significant difference in Mn between the 75 μM Cd treatment and the control (p > 0.05). The contents of carotenoid ranged between 3.06 and 3.26 mg/g across the different Cd treatments, showing no significant differences. The treatment with 5–75 μM Cd did not directly affect the photosynthesis of P. divaricata. Higher Cd concentrations reduced the stomatal density on the of P. divaricata leaves, resulting in stomatal and mesophyll conductance limitations, indirectly affecting P. divaricata photosynthesis. These research results provide a reference for evaluating and selecting heavy metal tolerant plants and provide environmentally friendly approaches to remediate heavy metal pollution.

Rancang Bangun Sistem Kontrol dan Monitoring Suhu dan Kelembaban Tanah pada Greenhouse berbasis Internet of Thing menggunakan Aplikasi Telegram

The quality of plant growth is significantly influenced by environmental factors, including temperature, air humidity, soil moisture, light intensity, and air quality. Therefore, an agrotechnology approach is required to manage exposure to these environmental factors in accordance with the needs of plant growth processes, which can be achieved through greenhouse technology. To facilitate real-time and remote monitoring and control in the greenhouse farming system, this research involves the design of a temperature and humidity control and monitoring system based on the Internet of Things (IoT). This study employed a NodeMCU ESP8266 microcontroller and a DHT11 sensor to detect the temperature and humidity levels in the greenhouse. The performance test results show that the system works well according to the programming algorithm provided, with an average error rate of 1.2% for the temperature sensor and 1.17% for the humidity sensor. The test results for notification delay time on the Telegram application also meet the standard requirements of Telecommunications and Internet Protocol Harmonization Over Networks (TIPHON), with an average delay value of 0.532882 ms

A Novel Extensible Continuum Robot with Growing Motion Capability Inspired by Plant Growth for Path-Following in Transoral Laryngeal Surgery.

This article presents a novel extensible continuum robot (ECR) with growing motion capability for improved flexible access in transoral laryngeal procedures. The robot uses an extensible continuum joint with a staggered V-shaped notched structure as the backbone, driven by the pushing and pulling of superelastic Nitinol rods. The notched structure is optimized to achieve a wide range of extension/contraction and bending motion for the continuum joint. The successive and uniform deflection of the notches provides the continuum joint with excellent constant curvature bending characteristics. The bidirectional rod-driven approach expands the robot's extension capabilities with both pushing and pulling operations, and the superelasticity of the driving rods preserves the robot's bending performance. The ECR significantly increases motion dexterity and reachability through its variable length, which facilitates collision-free access to deep lesions by following the anatomy. To further exploit the advantages of the ECR in path-following for flexible access, a growing motion approach inspired by the plant growth process has been proposed to minimize the path deviation error. Characterization experiments are conducted to verify the performances of the proposed ECR. The extension ratio achieves up to 225.92%, and the average distal positioning error and hysteresis error values are 2.87% and 0.51% within the ±120° bending range. Compared with the typical continuum robot with a fixed length, the path-following deviation of this robot is reduced by more than 58.30%, effectively reducing the risk of collision during access. Phantom experiments validate the feasibility of the proposed concept in flexible access procedures.

An atypical heterotrimeric Gα and its interactome suggest an extra-large role in overcoming abiotic and biotic stress.

Canonical heterotrimeric G-proteins (G-proteins) are comprised of Gα, Gβ, and Gγ subunits. G-proteins regulate multiple crucial plant growth and development processes, incorporating environmental responses. Besides Gα, Gβ and Gγ, the discovery of atypical Gα subunits termed as extra-large G-proteins or extra-large GTP-binding proteins (XLGs) makes G-protein signaling unique in plants. The C-terminus of XLG shares similarities with the canonical Gα subunits; the N-terminus harbors a nuclear localization signal (NLS) and is rich in cysteine. The earlier explorations suggest XLG's role in flowering, the development of embryos and seedlings, root morphogenesis, stamen development, cytokinin-induced development, stomatal opening and regulation of rice grain filling. The XLGs are also known to initiate signaling cascades that prime plants against a variety of abiotic and biotic stresses. They are also engaged in controlling several agronomic parameters such as rice panicle length, grain filling, grain size, and biomass, highlighting their potential contribution to crop improvement. The present review explores the remarkable properties of non-canonical Gα subunits (XLGs) and reflects on the various developmental, abiotic and biotic stress signaling pathways controlled by them. Moreover, the bottleneck dilemma of how a tiny handful of XLGs control a multiplicity of stress-responsive activities is partially resolved in this review by addressing the interaction of XLGs with different interacting proteins. XLG proteins presented in this review can be exploited to gain access to highly productive and stress-tolerant plants.

Un caso de transversalidad con regresiones y ecuaciones en el crecimiento de una planta de maíz amarillo (Zea mays L.)

For educational purposes for student learning and the cross-sectional relationship of mathematics with corn production, this research is developed as a case study of the Centro Bachillerato Tecnológico Agropecuario 116 (CBTa 116) located in the municipality of Angostura, Sinaloa. Corn is a fundamental product of the primary sector of the municipality and the state, therefore, the case study is directly linked to the context, thus allowing, to acquire an integral learning of the growth process of a yellow corn plant (Zea mays) and considering transversality as a focal point of mathematics, through research and implementation in the sowing of said seed through a quasi-experimental design. The process to follow was sowing, germination and monitoring the growth of the plant, recording the data in a logbook for 18 days. Subsequently, a table and graphs with regression results were developed to obtain its equation and R2 (attachment to the data) using Excel software, thereby generating exponential, linear, logarithmic and potential equations. The results obtained showed that the linear equations were the ones that were most attached to the growth that the plants showed with the equation y= 1.9319x + 0.4582 and with an R2 of 0.9893.

Changes in m6A RNA methylation are associated with male sterility in wolfberry

BackgroundN6-methyladenosine (m6A) modification is the most abundant type of RNA modification in eukaryotic cells, playing pivotal roles in multiple plant growth and development processes. Yet the potential role of m6A in conferring the trait of male sterility in plants remains unknown.ResultsIn this study, we performed RNA-sequencing (RNA-Seq) and m6A-sequencing (m6A-Seq) of RNAs obtained from the anther tissue of two wolfberry lines: ‘Ningqi No.1’ (LB1) and its natural male sterile mutant ‘Ningqi No.5’ (LB5). Based on the newly assembled transcriptome, we established transcriptome-wide m6A maps for LB1 and LB5 at the single nucleus pollen stage. We found that the gene XLOC_021201, a homolog of m6A eraser-related gene ALKBH10 in Arabidopsis thaliana, was significantly differentially expressed between LB1 and LB5. We also identified 1642 and 563 m6A-modified genes with hypermethylated and hypomethylated patterns, respectively, in LB1 compared with LB5. We found the hypermethylated genes significantly enriched in biological processes related to energy metabolism and lipid metabolism, while hypomethylation genes were mainly linked to cell cycle process, gametophyte development, and reproductive process. Among these 2205 differentially m6A methylated genes, 13.74% (303 of 2205) were differentially expressed in LB1 vis-à-vis LB5.ConclusionsThis study constructs the first m6A transcriptome map of wolfberry and establishes an association between m6A and the trait of male sterility in wolfberry.

Light signaling as cellular integrator of multiple environmental cues in plants.

Plants being sessile need to rapidly adapt to the constantly changing environment through modifications in their internal clock, metabolism, and gene expression. They have evolved an intricate system to perceive and transfer the signals from the primary environmental factors namely light, temperature and water to regulate their growth development and survival. Over past few decades rigorous research using molecular genetics approaches, especially in model plant Arabidopsis, has resulted in substantial progress in discovering various photoreceptor systems and light signaling components. In parallel several molecular pathways operating in response to other environmental cues have also been elucidated. Interestingly, the studies have shown that expression profiles of genes involved in photomorphogenesis can undergo modulation in response to other cues from the environment. Recently, the photoreceptor, PHYB, has been shown to function as a thermosensor. Downstream components of light signaling pathway like COP1 and PIF have also emerged as integrating hubs for various kinds of signals. All these findings indicate that light signaling components may act as central integrator of various environmental cues to regulate plant growth and development processes. In this review, we present a perspective on cross talk of signaling mechanisms induced in response to myriad array of signals and their integration with the light signaling components. By putting light signals on the central stage, we propose the possibilities of enhancing plant resilience to the changing environment by fine-tuning the genetic manipulation of its signaling components in the future.

Melatonin improves plant water status, photosynthetic performance, and antioxidant defense system in highbush blueberry (Vaccinium corymbosum L.) plants subjected to drought stress

Drought stress causes a decrease in agricultural yields. Recently, it has been reported that the phytohormone melatonin (MT), participates in different plant growth and developmental processes. Highbush blueberry (Vaccinium corymbosum L.) is quickly affected by drought, decreasing plant growth, yield, and quality. Thus, the aim of this study was to evaluate the role of melatonin on physiological performance, water status, chlorophyll concentration, and antioxidant metabolism in V. corymbosum plants exposed to drought stress. One-year-old V. corymbosum plants were subjected to four treatments: 1) 100% field capacity (FC) without melatonin; 2) 100% FC with 0.1 mM MT; 3) 50% FC without MT; and 4) 50% FC with MT. Plant water content, leaf area, photosynthetic performance, antioxidants compounds, and chlorophyll concentration were determined at different times post MT application (0, 3, 7, and 10 days). In our study, leaf area, plant water status, the photosynthetic performance and photosynthetic pigments decreased in the treatments with irrigation at 50% FC compared to the treatment whit irrigation at 100% FC. Interestingly, MT application improved plant water potential, CO2 assimilation, chlorophyll a, and total phenols by 35, 65, 22, and 27% respectively, at 50% FC treated with MT compared to plants without MT at 10 days of the experiment. Our results demonstrate that MT enhanced physiological and biochemical responses of V. corymbosum plants to cope with drought stress.

The Effect of Low Temperature Stress on Plant Physiological Development

Temperature is the most important factor affecting plant growth, development, and metabolic processes, which has significant theoretical value and practical significance for studying the physiological and biochemical characteristics of plants under temperature stress. The damage of low temperature to plants is caused by dehydration caused by extracellular ice formation, or by apoptosis caused by intracellular ice formation. This article mainly discusses the cold resistance of plants from several aspects, including lipid membrane peroxidation, osmotic regulation products, plant growth and development products, and antioxidant enzyme systems.

On the continuum mechanics of growing plant-like structures

The contribution at hand focuses on the introduction of a novel approach to model biological growth. The proposed formulations are chosen to represent plant like structures. Therefore, thermomechanically open systems are considered. The balance laws are presented for such systems. Furthermore, the proposed formulations are coupled with an adaptive meshing framework. Therefore, a so-called structural generator is presented and utilized in this work. Since no growth formulations within the framework of continuum mechanics exist so far for plant like systems, a novel set of constitutive equations is shown. The newly described principles are the phototropism and graviotropism. In the numerical examples, it is shown that the proposed formulation yields physically meaningful results. The combination of different growth principles results in plausible interactions of the aforementioned principles. Furthermore, results of numerical simulations are shown, which represent the growth process of plant like biological structures.

MsCYP71 is a positive regulator for drought resistance in alfalfa

Cytochrome P450 (CYP450) family proteins play key roles in plant growth, development, stress responses, and other physiological processes. Here, we cloned the cytochrome P450 gene MsCYP71 in alfalfa and found that the expression of MsCYP71 was induced by drought stress. Silencing the MsCYP71 gene using virus-induced gene silencing technology significantly decreased the drought resistance of alfalfa, as indicated by their lower relative water content, net photosynthetic rate, and chlorophyll fluorescence maximum (Fm); further, the heterologous overexpression of MsCYP71 in tobacco significantly enhanced the drought resistance and Fm of transgenic tobacco. Furthermore, the expression of MsCYP71 across 45 alfalfa accessions under drought stress was investigated. A significant positive correlation between drought resistance and MsCYP71 expression was observed. The 45 alfalfa accessions were clustered into four groups, and drought resistance, Fm, and MsCYP71 were higher in group I than in the other groups, indicating that group I accessions can be used as candidate germplasm resources for the breeding of drought-resistant alfalfa varieties. Overall, our findings indicated that MsCYP71 is a positive regulator of drought resistance in alfalfa, and its expression can be used to evaluate the drought resistance of alfalfa.

Transcriptomic insights of Auxin Transport in Gymnema sylvestre R. Br

Gymnema sylvestre R. Br. is an important medicinal plant with antidiabetic properties. This is a non-model, slow growing medicinal plant and, except a transcriptome study, no genomic data is available in public domain. Understanding on cellular transport at transcriptome level was investigated for auxins being important plant hormone modulating many growth and developmental processes in plants. From a huge transcriptome date, we reported four genes Auxin Influx Carriers, Transport Inhibitors Response 1, Auxin Efflux Carriers and Auxin Response Factor. The sequences of these genes were used to prepare phylogeny of the nearest gene available in public domain and the model of the protein was predicted based on amino acid sequences. The analysis revealed that Auxin Influx Carriers in G. sylvestre (MT911901) is made up of 1461 bases and shares maximum similarity with Coffea eugenioides auxin transporter-like protein 2 (XM_027319767.1). Out of the five models generated through i-TASSER, the first model had C-score = −1.18, estimated TM-score = 0.57 ± 0.15 and the estimated RMSD = 10.0 ± 4.6 Å. The Transport Inhibitors Response 1 in G. sylvestre (MT911902) is made up of 1761 bases and is most similar to the Coffea eugenioides Transport Inhibitor Response 1 (XM_027304015.1). From this transcriptome analysis in G. sylvestre, three coding DNA sequences encoding PINs (two encoding PIN3; OP494270 and OP494271, one encoding PIN8; OP494272) and Auxin Response Factor (OP515523) are identified and submitted to the NCBI gene repository.

Genome-wide identification of HSF gene family and their expression analysis in vegetative tissue of young seedlings of hemp under different light treatments

Heat shock transcription factors (HSFs), play a crucial role in plant growth, development and defense processes. The functions of some HSFs have been well studied in response some stresses in model plants. At present, the genome of the medicinal plant Cannabis sativa L. has been fully sequenced. Nevertheless, there is little known about the HSF genes family in Cannabis sativa L, as well as a lack of understanding of its evolutionary history. Herein, 18 HSFs were identified in the cannabis genome and phylogenetically classified into three groups based on the structural characteristics and phylogenetic analysis, including classes A (9 genes), classes B (8 genes), and classes C (1 gene). They were distributed over 9 of 10 chromosomes without chromosome 8. Then, the motif composition and evolutionary relationship of CsHSF proteins are discussed in detail, along with gene structures, cis-acting elements in the promoter, and synchronization analysis. Furthermore, our results also revealed that CsHSF transcription levels varied significantly among tissues, suggesting that the functions of CsHSF may be different. Finally, we found that red and blue light treatments had a significant effect on HSF genes expression and cannabinoids accumulation in vegetative organs of hemp young seedlings, implying the expression of HSF genes may regulate the content of cannabinoids under different light conditions. Although cannabinoid biosynthesis and response to light in vegetative organs of hemp young seedlings may not represent the mature stage of hemp, these results provided an essential theoretical basis to elucidate the regulatory mechanism of HSF in cannabinoid biosynthesis in the future.