GH Gene Research Articles

Reducing the modifying impact of the ecological factor at breeding value assessment of Hereford bull calves taking into account phenotype and complex of genes GH and GDF5

The objective assessment of beef cattle genetic potential is a priority task of beef cattle breeding intensification. The purpose of the study was to test a method for reducing the modifying effect of ecological factor in assessing the breeding value of Hereford bulls based on combined selection, taking into account the phenotype assessment and genotyping by GH and GDF5 genes. For this purpose, the bull calves were tested according to productivity in two variants: 1) setting and selection of young cattle for control cultivation on the basis of phenotype (traditional assessment); 2) traditional selection was supplemented by genotyping assay for the complex of GH and GDF5 genes. The individuals with desired allelic profile (genotypes VV and VL of GH gene and genotypes TT and TC of GDF5 gene) showed increased weight gain intensity relative to their herd mates. The analysis of body weight repeatability data at different stages of ontogenesis shows that the selection of carriers of desirable alleles for the gene complex increases the accuracy of high-priced animal selection. Thus, in order to determine the genetic potential of Hereford bull calves more objectively and minimizing the ecological impact on breeding value, it is advisable to use a combined approach to selection that unites the assessment of the phenotype of young cattle and the genotyping of animals taking into account the complex of genes GH and GDF5.

Characterization and expression of DNA sequences encoding the growth hormone gene in African Pygmy Mouse (Mus minutoides).

We determined the nucleotide sequence of the growth hormone (Gh) gene in Mus minutoides, one of the smallest mammals, where was predicted to be distinct in the functional regions between M. minutoides and Mus musculus. To investigate the evolutionary characteristics of Gh in M. minutoides, we constructed a phylogenetic tree based on the putative amino acid sequences of Gh, suggesting that the Gh of M. minutoides diverged earlier than M. musculus. Furthermore, the Gh gene expressed higher in M. minutoides than in M. musculus. Our results suggest that the specific feature of the Gh in M. minutoides is in rather the regulatory mechanism than the sequence.

Polymorphism Exploration of Growth Family (GH, GHRH and PIT-1) Genes Polymorphisms of Local Swamp Buffalo for Productivity Improvement in North Tapanuli Regency, North Sumatra

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. Genetic polymorphism of the growth family (GH, GHRH, and PIT1) genes were studied in local swamp buffalo (106 hds.) from a government buffalo breeding station (46 heads) and smallholders (60 heads) in North Tapanuli District, North Sumatra Province. Genotype variants of the three genes were identified by PCR-RFLP method using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene) and HinfI (PIT -1 gene). Genotyping on individual GH_g.1547T>C, GHRH_g.4666G>C, and PIT -1_g.1256G >A loci resulted only one type genotype, respectively TT, CC, an d AA, with one type of allele, respectively T, C, and A. Heterozygosity observation (Ho) and expectation (He) values values and the PIC value for each locus was 0.00. It could be suggested to increase genotype frequenciest of the three growth genes that are positively associated with the growth traits and economic traits of the buffalo.

Identification of growth hormone gene polymorphism and its association with body weight in PO Kebumen cattle

PO-Kebumen cattle was one of local genetic resources that famous with good body performance and high growth rate. The aim of this research was to detect growth hormone (GHR, GH and Pit-1) polymorphism and its association with body weight performance of PO-Kebumen cattle. The sample used in this research was 69 heads PO-Kebumen cattle belongs to the farmers of PO-Kebumen Cattle Farmer Association (ASPOKEB). This research observed the growth of PO Kebumen cattle, birth weight, weaning weight and the weight at one year. The parameters observed were birth weight, weaning weight and the weight at one year. The DNA extraction was carried out using blood samples. After DNA extraction, PCR-RFLP method was used to observe the genotype of three growth gene (GH, GHR and Pit1). The results showed that the GH and GHR genes were polymorphic, meanwhile Pit1 gene was monomorphic. The analysis showed that the three growth genes were not significantly associated to body weight (birth weight, weaning weight and the weight at one year) on PO-Kebumen cattle.

Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

Lactation traits are controlled by many genes, among others, potentially by growth genes. This research was conducted to study genetic polymorphisms of GH, GHRH, and Pit-1 genes and associations of GH (g.1456_1457 InsT), GHRH (g.4474 C>A), and Pit-1 (g.244 G>A) genotypes with milk yield and quality in Holstein Friesian (HF) cattle. Genotyping was conducted for HF dairy cows raised by small farmers from North Lembang (SF-NL) (98 heads) and South Lembang (SF-SL) (95 heads), and also from Cikole Dairy Cattle Station (CDCS) (82 heads) in Lembang, West Java, Indonesia. Progeny tested of HF bulls (17 heads) from LAIC (Lembang Artificial Insemination Center) in West Java and from SAIC (Singosari AIC) (32 heads) in East Java were also genotyped. Effects of genotypes on test day milk yield, fat content, and SNF content were analyzed by General Linear Models. The GHRH g.4474 C>A SNP and Pit-1 g.244 G>A SNP generated high frequencies of C allele to A allele, while the two allelic frequencies of the GH g.1456_1457 InsT varied. Compared to the AA and AC genotypes, the CC genotype of the GH gene resulted higher test day milk yield (p<0.01), fat content (p<0.05), and SNF content (p<0.05). Further the CC genotype of the GHRH gene yielded higher milk yield (p<0.05), while the GG genotype of the Pit-1 gene resulted higher fat content (p<0.05). Therefore the GH g.1456_1457 InsT, GHRH g.4474 C>A SNP, and Pit-1 g.244 G>A SNP are potential to be used as molecular markers for selection on milk yield and quality in domestic HF cattle.

Study the Effect of Microinjection of Zn, Fe, and Cu Loaded in Montmorillonite on Development Activities in Fertilized Chicken Eggs.

This study aims to reduce embryonic mortality, increase body weight, and improve immune system in chicken. A total of 240 eggs were assigned to three treatments (n = 60) and injected with cooper (Cu), zinc (Zn), and iron (Fe) loaded by montmorillonite (Mnt), and one untreated group (n = 60). Some hormones and enzymes related with growth were measured in terms of serum, and expression of some genes related to growth, immune, and programmed cell deaths that were determined in the liver and spleen of chicken by RT-qPCR. The embryonic death on the fifth and seventh days after injecting eggs with Fe-Mnt was less obvious than in other groups. The heaviest body weight was recorded for Fe-Mnt and Cu-Mnt treatment. Fe-Mnt treatment had higher serum GSH, SOD, GH, and Myostatin contents and lower MDA than those in the other treatments. Cu-Mnt treatment included the highest contents of CAT enzyme and IGF-1 hormone in serum. The highest expression of IGF-1, GH, BCL6, and SYK genes in liver tissue were recorded by Zn-Mnt, IGFBP2, FGF8, and IFNW1 genes by Cu-Mnt, and TC1RG1 and IFNW1 genes by Fe-Mnt in spleen tissue. In conclusion, Fe-Mnt was the best treatment for reducing embryonic mortality, and increasing body weight of chickens and expression of growth and immune genes, followed by Cu-Mnt treatment.

Endothelial dysfunction in patients with acromegaly and It's association with Endocan

ObjectiveThis study aims to assess endocan levels in patients with acromegaly who have active disease or disease in remission and to investigate a relation between endocan levels and endothelial dysfunction in these patients. DesignThe study is a case-control study. Study was conducted at Istanbul Medeniyet University Goztepe Training and Research Hospital between 2013 and 2019. Patients who were older than 18 years with acromegaly diagnosis were recruited if they agreed to participate. Patients with uncontrolled diabetes (DM), hypertension (HT), hyperlipidemia, decompensated heart failure, immune or infectious diseases, moderate-severe valve disease and stage 3 or more advanced chronic kidney disease were excluded. There were 30 healthy control subjects who agreed to participate to the study. Patients with acromegaly were divided into two groups as: disease active patients and patients in remission. Serum endocan levels were measured with enzyme linked immunosorbent assay (ELISA) method endothelial function was assessed with flow mediated dilatation (FMD). ResultsThere were 85 patients included to the study. Twenty-three patients had active disease, 31 were in remission and 31 were healthy controls. FMD was higher in controls compared to patients in active disease and patients in remission (p < 0.001). There was no difference between patients with active disease for FMD and patients in remission (p = 0.088). There was statistically significant correlation between FMD and endocan and insulin like growth hormone-1 (IGF-1) levels of patients with acromegaly. As FMD increased endocan and IGF-1 decreased. A moderate negative relation between FMD and endocan was identified (p < 0.001, r:-0.409) as well as FMD and IGF-1 levels (p:0.011, r:-0.377). Along with endocan and IGF-1, DM, HT, sex, body mass index, age and uric acid were associated with changes in FMD. ConclusionsEndocan levels and endothelial function measured with FMD have an inverse relationship. Endocan may prove to be a marker for endothelial dysfunction in acromegaly.

Evaluating the possible feeding strategies of selenium nanoparticles on the growth rate and wellbeing of European seabass (Dicentrarchus labrax)

The study aimed at evaluating the possible feeding strategies of selenium (Se) nanoparticles on the performances of European seabass (Dicentrarchus labrax). The first group fed the basal diet continuously (control) while the second group offered Se (1 mg/kg diet) every day (R1). The third group fed the basal diet one day then Se diet every other day (R2), and the fourth group basal diet for two days then fed Se diet every two days (R3). The FBW, WG, and SGR were significantly improved in the R1 group than fish in the other groups (P < 0.05). The FCR was significantly reduced by feeding Se nanoparticles to fish in R1, R2, and R3 groups than the control with the lowest being in R1 group (P < 0.05). The R1, R2, and R3 groups had enhanced values of Hb, RBCs, and WBCs (P < 0.05). Additionally, the blood total protein and globulin of fish fed in R1, R2, and R3 groups was significantly higher than the control (P < 0.05). Phagocytic index, phagocytic, lysozyme activities were significantly higher in fish fed Se nanoparticles (R1, R2, and R3 groups) than fish fed the basal diets (P < 0.05). The antioxidative related indices (SOD, CAT, and GPx) were enhanced in fish of R2 group while the concentration of MDA was reduced (P < 0.05). Further, the level of MDA was decreased in fish of R1 group that fed dietary Se nanoparticles every day. The relative expression of liver GH, IGF-1, IL-8, and IL-1β genes were significantly upregulated in fish of R1, R2, and R3 groups while HSP70 gene was reduced (P < 0.05). In conclusion, fish fed dietary Se nanoparticles at 1 mg/kg either daily, every other day, or every other two days had enhanced growth rate, feed efficiency, haemato-biochemical indices, immunity, antioxidative responses, and anti-inflammatory impacts on European seabass.

Exon and Intron Variability in Salmonidae Growth Hormone Genes

Gene duplication is one of the main mechanisms of formation of new genetic material in evolution. The occurrence of a gene duplication is believed to relax selection pressure on one of the copies. Consequently, this gene accumulates mutations at a higher rate, and over time it acquires a new function. As a result of several rounds of polyploidization, many genes in salmon are duplicated, including the growth hormone gene. The analysis of nucleotide diversity in the paralogous genes of growth hormone, gh1 and gh2, demonstrated that the level of variability in their introns was higher than in the exons. In addition, the variability of each exon weakly correlated with its length, and seems to be determined by the functional significance of the protein region encoded. The level of variability in the exons of the gh2 gene was higher than that in the gh1 one, which was probably due to the current process of gene subfunctionalization.

Indigenous Lien Minh chicken of Vietnam: Phenotypic characteristics and single nucleotide polymorphisms of GH, IGFBP and PIT candidate genes related to growth traits

Abstract. Thu HDT, Binh NTT, Duc LD, Huu DB, Nhung DT, Xuan CN, Viet LN, Thai AN, Quang ML, Pham DK, Hoang TN. 2020. Indigenous Lien Minh chicken of Vietnam: Phenotypic characteristics and single nucleotide polymorphisms of GH, IGFBP and PIT candidate genes related to growth traits. Biodiversitas 21: 5344-5352. Lien Minh chicken, a Vietnamese indigenous breed, has contributed importantly to the economic development of rural citizens in Cat Ba island, Hai Phong province due to its highly desired characteristics. However, Lien Minh chickens showed a slower growth rate when compared to other industrial chicken breeds. Therefore, the study aims at assessing the appearance characteristics and growth performance of 100 individuals Lien Minh chickens, and investigating the single nucleotide polymorphisms (SNPs) of candidate genes including GH, IGFBP and PIT, which might be associated with growth traits. Blood samples were collected from wing of Lien Minh chickens and used for DNA extraction. Genotyping by the PCR-RFLP method was then conducted. The allele frequencies were 0.97 and 0.03 for alleles A and G of GHi3 gene, respectively. For in IGFBP2 gene, allele frequencies were found as 0.47 for the A allele and 0.53 for G allele. The allele frequencies for PIT1 gene were recorded as 100% for allele B. These polymorphic loci (GHi3, PIT1, and IGFBP2) were followed by the Hardy-Weinberg equilibrium in the Lien Minh chicken population. These were the initial results, which could be used to analyze the correlation of molecular markers and growth traits in Lien Minh chickens.

Identification of GH1 gene family fgt members in Stevia rebaudiana and their expression when grown in darkness.

Stevia rebaudiana Bertoni is an important economic crop that is well known for its secondary metabolites, steviol glycosides (SGs), found in leaves. Because the enzymes of deglycosylation (glycoside hydrolases) play important roles in SGs biosynthetic processes, our study is focused on the functions of β-glucosidases in SGs catabolism in stevia. We cloned and characterized 19 stevia GH1 genes based on transcriptomic sequences. The 19 genes were divided into five putative subfamilies in Arabidopsis. Conserved motifs in the SrGH1 proteins were analysed using the online motif-based sequence analysis tool, MEME. Most of the identified proteins contain the conserved 'TFNEP' motif (contains the catalytic acid/base) and 'ITENG' motif (contains the catalytic nucleophile). Furthermore, the steviol glycoside content and expression of these 19 genes were characterized under constant darkness. The dark treatment lowered the steviol glycoside content significantly, while SrBGLU16 responded to darkness and was markedly upregulated. This study is the first transcriptome-wide analysis of the GH1 family in Stevia rebaudiana. The sequences of 19 SrGH1 members and their expression when grown in darkness were characterized. Among the 19 genes, SrBGLU16 was markedly upregulated by darkness. Thus, we identified SrBGLU16 for further investigation as a possible steviol glycoside beta-glucosidase.

Retraction: Increased maternal serum placental growth hormone variant in pregnancies complicated by otosclerosis

Retraction: Increased maternal serum placental growth hormone variant in pregnancies complicated by otosclerosis

A common deletion in the growth hormone receptor gene (d3-GHR) in the offspring is related to maternal placental GH levels during pregnancy

BackgroundA common growth hormone receptor polymorphism with deletion of exon 3 (d3-GHR) has previously been linked to increased postnatal growth on the one hand and decreased fetal growth on the other. Regulation of fetal growth is positively dependent on secretion of placental GH (hGH-V). ObjectiveWe explored the effect of the fetal d3-GHR genotype on maternal serum levels of hGH-V and fetal growth. The cellular localization of hGH-V synthesis and the GH receptors were determined in normal placentas. Methods43 healthy mother-child pairs were examined during pregnancy with measurements of hGH-V during third trimester, and serial ultrasound measurements determined fetal growth rate. Birth anthropometrics were obtained. The GHR genotype of the child was analysed postnatally. Immunohistochemical (IHC) analysis was conducted on four placentas. ResultsThe presence of the d3-GHR genotype was associated with a markedly reduced concentration of hGH-V in maternal serum (β −0.52, SE 0.24, p = 0.04) compared to those who had a fl/fl genotype. Accordingly, a tendency towards reduced fetal growth rate during third trimester (β −25.8, SE 12.7, p = 0.05) and a lower birth weight were found among carriers of the d3-GHR allele, but these associations did not reach statistical significance (p = 0.08). IHC analysis showed expression of placental GH and GHR in the villous syncytiotrophoblast, the extravillous trophoblast, and the decidual cells and smooth muscle cells in chorionic vessels. ConclusionsThe presence of the d3-GHR polymorphism in the fetus was associated with lower maternal serum levels of hGH-V, decreased fetal growth rate in third trimester and lower birth weight compared to the wildtype.

Selenium Nanoparticles Act Potentially on the Growth Performance, Hemato-Biochemical Indices, Antioxidative, and Immune-Related Genes of European Seabass (Dicentrarchus labrax).

The current study investigated the role of selenium (Se) nanoparticles on the growth performance, hemato-biochemical indices, antioxidative, and immune-related genes of European seabass (Dicentrarchus labrax). Therefore, fish with initial weight of 20.53 ± 0.10 g/fish werefed diets with 0, 0.25, 0.5, and 1 mg Se nanoparticles/kg diet for 90 days. The final body weight, weight gain, and specific growth rate of fish fed dietary nano-Se varying levels were significantly higher than the control with the highest performances and lowest FCR in the group of fish fed nano-Se at 0.5mg/kg. The values of Hb, PCV, RBCs, and WBCs were significantly higher in fish fed varying levels of Se nanoparticles than fish fed the basal diets. The values of total serum protein and globulin were significantly higher in fish fed varying levels of Se nanoparticles than fish fed the basal diets. Additionally, globulin had higher value in the group of fish fed 0.25 and 0.5mg nano-Se/kg than fish fed 1mg nano-Se/kg (P < 0.05). No significant alterations were observed on albumin, ALT, and AST variables (P > 0.05). Phagocytic index, phagocytic, lysozyme activities were significantly higher in fish fed varying levels of Se nanoparticles than fish fed the basal diets in a dose dependent manner (P < 0.05). Further, SOD activity had higher value in the group of fish fed 0.25 and 0.5mg nano-Se/kg than fish fed 1mg nano-Se/kg, whereas CAT was increased in the group of fish fed dietary 0.5mg nano-Se/kg diet (P < 0.05). The level of MDA was significantly lowered by dietary nano-Se where the group of fish fed 0.25mg/kg had the lowest level followed by those fed 0.5 and 1mg/kg. The expression of GH, IGF-1, IL-8, and IL-1β genes had the highest mRNA levels in the group of fish fed 0.25 and 0.5mg/kg followed by those fed 1mg/kg, whereas HSP70 was downregulated. Based on the overall results, Se nanoparticles are recommended at the rate of 0.5-1mg/kg diet to maintain the optimal growth performance, hemato-biochemical indices, antioxidative status, and immune-related genes in European seabass.

Activin A affects feeding by promoting the inner diameter and muscle development of the pharynx and oesophagus in zebrafish (Danio rerio) larvae.

Activin A belongs to the superfamily of transforming growth factor-β and plays an important role in hormone regulation and tissue development. However, few research studies have been conducted on the effect of activin A on feeding organs in fish. In this study, the zebrafish (Danio rerio) larvae were treated with 1 ng ml-1 activin A for 8 days continuously. The haematoxylin and eosin (H&E) staining section results revealed that the transverse inner diameter of the pharynx and oesophagus significantly increased on the third and eighth days after treatment compared with the control group (P < 0.05). On the eighth day, the cross-sectional area of the pharyngeal muscle increased by 8638 μm2 compared to the control group (P < 0.05). The RNA in situ hybridization results also showed that the expression of skeletal muscle-specific genes (myog and myod) was significantly increased in pharyngeal muscle on the eighth day. Furthermore, the qRT-PCR results showed the expression of gh gene was significantly increased on the eighth day (P < 0.05). At the same time, more larvae in activin A group were able to feed larger brine shrimp (Artemia) than in the control group on the eighth day. In conclusion, activin A could affect feeding by promoting the inner diameter and muscle development of the pharynx and oesophagus in zebrafish larvae. This study is the first to report that the development of the pharynx and oesophagus can directly affect food intake in fish larvae, which provides a theoretical basis for the study of food intake of fish at an early stage.

Enteromorpha prolifera Diet Drives Intestinal Microbiome Composition in Siganus oramin.

Enteromorpha prolifera (E. prolifera) contains complex sulfated polysaccharides that are resistant to biological degradation. Most organisms cannot digest biomass of E. prolifera, except Siganus oramin (S. oramin). This study was conducted to identify the bacteria in the intestine of S. oramin facilitating the digestion of E. prolifera polysaccharides (EPP). Metagenomic sequencing analysis of the S. oramin intestinal microbiota revealed that E. prolifera diet increased the number of Firmicutes, replacing Proteobacteria to be the dominant bacteria. The proportion of Firmicutes increased from 38.8 to 58.6%, with Bacteroidetes increasing nearly fivefold from 5 to 23.7%. 16S rDNA high-throughput sequencing showed that EPP-induced Bacteroidetes increased significantly in the intestinal flora of S. oramin cultivated in vitro. Metatranscriptome analysis showed that EPP induced more transferase, polysaccharide hydrolase, glycoside hydrolase, and esterases expressed in vitro, and most of them were taxonomically annotated to Bacteroidetes. Compared with the aggregation of GH family genes in metagenomic sequencing analysis in vivo, EPP induced more CBM32, GH2, GT2, GT30, and GH30 families gene expression in vitro. In general, We found that the bacteria in intestinal tract of S. oramin responsible for digestion of E. prolifera were Firmicutes and Bacteroidetes, while Bacteroidetes was the dominant bacteria involved in EPP degradation in vitro cultures. Compared with in vivo experiments, only GH family genes were mostly involved, we detected a more complete and complex EPP degradation pathway in vitro. The results may benefit the further study of biodegradation of E. prolifera and has potential implications for the utilization of E. prolifera for biotechnology.

Genetic and Hormonal Difference between High Growth Rate Breed (Cobb Broiler chicken) and Low Growth Rate Breed (Native Fayoumi chicken)

Abstract The present study aimed at investigating the genetic and hormonal differences between a low growth rate chicken breed (a native breed: Fayoumi chicken) and a high growth rate chicken breed (Cobb broiler chicken). A total number of 100 one-day-old Fayoumi chicks and 100 one-day-old Cobb chicks were used. Chicks of each breed were allocated into three equal replicates. The growth parameters (body weight, BWG and FCR), genetic expression of some growth related genes (GH, IGF1, ghrelin and Myostatin gene) and plasma level of some growth related hormones (Insulin, T3, T4 and corticosterone) were recorded. The obtained results revealed that, Cobb chicken had significantly higher body weight, BWG and feed intake than Fayoumi chicken but the FCR of Fayoumi chicken was higher than that of broiler chicken. Cobb recorded significantly lower plasma level of corticosterone, T3 and T4 than Fayoumi chicken while Insulin is higher in cobb chicken. Cobb recorded highly significant values of GH, IGF1 and ghrelin Gene expression while myostatin gene expression was higher in Fayoumi chicken than in Cobb From the obtained results could be concluded that, the observed high growth rate in cobb chicken may be attributed the difference gene expression related growth as well as hormonal difference

Effect of Dietary Sodium Acetate on Skin Mucus Immune Parameters and Expression of Gene Related to Growth, Immunity and Antioxidant System in Common Carp (Cyprinus carpio) Intestine

Abstract The present study investigated the possible effects of including salt of short chain fatty acid, sodium acetate (SA), on skin mucus immune parameters and immune, antioxidant and growth-related genes expression in common carp. There is little data available about the effective role of SA on immune, antioxidant and growth related genes expression as well as skin mucus immune parameters. The aim of this study was to analyse the effect of SA intake on these factors using common carp (Cyprinus carpio) as model organism. Two hundred and forty healthy common carps (mean weight = 15 ± 0.9 g) were supplied and randomly stocked into 12 fiberglass tanks 200 L (20 fish per tank) assigned to four treatments and triplicates. The study was performed in a completely randomized design. The treatments were feeding carps with experimental diets containing different levels (0.0 [control], 0.5, 1 and 2%) of SA. The skin mucus total immunoglobulin and total protein levels in fish fed 2% SA showed significant increase compared to the control group (P&lt;0.05). Results showed a significant increase in the GH gene expression in 1 and 2% SA treatments (P&lt;0.05). The carps fed the diet with 2% SA showed a significant increase in IGF-1 expression (P&lt;0.05). The expression of GSTa, and GPX (antioxidant genes) revealed a significant increase in the GSTa (fish fed SA at 1% and 2% levels) and GPX gene expression with fish fed 2% SA (P&lt;0.05). Supplementation of fish diet with SA induced a slight elevation in the intestine of all immune-related genes (TNF-α, IL-1β, IL8 and Lyz) compared to the control group (P&gt;0.05). However, Lyz gene was significantly up-regulated in 1 or 2% SA treatments. These results confirmed beneficial effects of SA as a feed additive in common carp culture.

Association of lactation persistency with genetic variants of bovine growth hormone gene in Indian Sahiwal cows

The aim of this study was to determine the associations of persistency with genetic variants of bovine growth hormone (bGH) gene in Indian Sahiwal cows. The data pertaining to the monthly test day milk yield (MTDMY) and first lactation length (FLL) of 305 lactating Sahiwal cows over a period of 23 years (1993–2016) were subjected to analysis. Persistency of milk yield was estimated by using test day based ratio method and Wilmink b function. Mean LL was 220.06±8.15 days in all studied Sahiwal cows, however, 276.67±10.25 days in cows with ≥100 days of LL and having 500 kg MY. The peak of milk yields was achieved at third TD of the first lactation. Macrophage stimulating protein 1 (MspI) and Arthrobacter luteus1 (AluI) restriction endonucleases were used to digest the amplified regions in intron 3 and exon 5, respectively of bGHgene. The bGHMspI restriction fragment length polymorphism (RFLP) yielded TT, CT and CC genotypes, whereas bGH-AluIRFLP yielded CC, CG and GG genotypes. Significant association was found between first lactation persistency estimated using test day based ratio method and bGH-MspI polymorphism. No significant association was found between first lactation persistency and bGH-AluI polymorphism. In summary, results of present study revealed that the TT genotype of bGH–MspI gene in Sahiwal cows has better lactation persistency than other genotypes.

The 20-kDa Placental GH Variant: A New and Improved Growth Hormone?

The 20-kDa Placental GH Variant: A New and Improved Growth Hormone?