Pleurotus Ostreatus Research Articles

The Potential of Mycofoam as a Biocomposite Material with Various Substrate and Mushroom Compositions

Mycofoam is a biocomposite composed of mycelial filaments that bind to the host substrate, which can replace polystyrene. This research aims to determine the most appropriate formulation of the growing medium and mushroom to be used in mycofoam products based on some parameters. Composition is one of the main factors for selecting the most appropriate mycofoam to replace polystyrene. On the other hand, the quality of mycofoam can be assessed through its strength and water resistance. The mushroom mycelium used in this study came from Pleurotus ostreatus and Lentinula edodes. Besides, the growing medium formulation comprises five different compositions, each containing different proportions of sawdust and bagasse. This research was composed of preparation of growing medium, spawn inoculation, molding, heating, testing, and data analysis. Based on the results, the combination of 100 percent sawdust and L. edodes was the most appropriate choice compared to other formulations based on strength and appearance. The results of the water absorption test showed that all mycofoam formulations were not resistant to water, bio-based coating can be used to overcome this problem.

Effect of Five Concentrations of Aqueous Extracts of Pleurotus ostreatus P. Kumm and Tagetes minuta L. on the Mortality of Two Nematodes in a Laboratory Setting

The nematode attacks affect several plant species of Ecuadorian crops. There are fungi and plants with nematicide ability that have agricultural interest. This study assessed the effect of five concentrations of aqueous extracts of Pleurotus ostreatus and Tagetes minuta on the mortality of Meloidogyne spp. and Panagrellus redivivus nematodes in a laboratory setting. The aqueous extracts were obtained through hydro distillation where concentrations of 0%, 0.5%, 5%, 25%, 50% and 100% were prepared. A wholly randomized single-factor design was used for the P. ostreatus extract and a bifactorial for the T. minuta extract (leaves and flowers). The number of dead individuals was evaluated, and the efficacy and LC50 were determined. T. minuta leaf extract showcased higher nematicide activity against P. redivivus with an LC50 of 8.03 ppm; when applied to Meloidogyne sp., the extract showed nematicide activity with an LC50 of 0.01 ppm. For P. ostreatus extract, the greatest nematicide activity against P. redivivus was an LC50 of 1.22 ppm and nematicide activity against Meloidogyne sp., was an LC50 of 0.01 ppm. The aqueous extract of T. minuta flowers showed low nematicide activity and the aqueous extract of T. minuta leaf showed the best nematicide activity. Keywords: nematicide; Tagetes minuta; Pleurotus ostreatus; Panagrellus redivivus; Meloidogyne sp.

Effect of Five Concentrations of Aqueous Extracts of Pleurotus ostreatus P. Kumm and Tagetes minuta L. on the Mortality of Two Nematodes in a Laboratory Setting.

The nematode attacks affect several plant species of Ecuadorian crops. Fungi and plants with nematicide ability are of agricultural interest. This study assessed the effect of five concentrations of aqueous extracts of Pleurotus ostreatus and Tagetes minuta on the mortality of Meloidogyne spp. and Panagrellus redivivus nematodes in a laboratory setting. The aqueous extracts were obtained through hydro distillation where concentrations of 0%, 0.5%, 5%, 25%, 50% and 100% were prepared. A wholly randomized single-factor design was used for the P. ostreatus extract and a bifactorial for the T. minuta extract (leaves and flowers). The number of dead individuals was evaluated, and the efficacy and LC50 were determined. T. minuta leaf extract showcased higher nematicide activity against P. redivivus with an LC50 of 8.03 ppm; when applied to Meloidogyne sp., the extract showed nematicide activity with an LC50 of 0.01 ppm. For P. ostreatus extract, the greatest nematicide activity against P. redivivus was an LC50 of 1.22 ppm and nematicide activity against Meloidogyne sp., was an LC50 of 0.01 ppm. The aqueous extract of T. minuta flowers showed low nematicide activity and the aqueous extract of T. minuta leaf showed the best nematicide activity. Keywords: nematicide; Tagetes minuta; Pleurotus ostreatus; Panagrellus redivivus; Meloidogyne sp.

Evaluation of Sample Size Influence on Chemical Characterization and In Vitro Antioxidant Properties of Flours Obtained from Mushroom Stems Coproducts.

The mushroom industry generates large amounts of stem co-product. This is generated after mushroom harvest; stems are attached to the growth substratum, and their only use has traditionally been as compost. In this study, we investigated extensively for the first time this co-product and the influence of sample size (L->0.510 mm; LI-0.510-0.315 mm; SI-0.315-0.180 mm; S-<0.180 mm) on the characterization and antioxidant activity of flours obtained from stem co-products of Agaricus bisporus (ABSF) and Pleurotus ostreatus (POSF). ABSF was rich in protein (14 g/100 g), calcium (428.23-700.77 mg/100 g), and sorbitol (22.57-26.60 g/100 g), while POSF was rich in β-glucans (36.62-40.34 g/100 g) and linoleic acid (20.57-39.86 g/100 g of lipid). Both species were flush in amino acids and had an umami flavour. ABSF showed more elevated values for emulsifying activity than POSF. The S sizes were highlighted for their yield, hydration properties, and oil holding capacity. Furthermore, ABSF-S exhibited heightened antioxidant capacity in vitro, in consonance with the total phenolic compounds observed (0.91 mg/g). However, the antioxidant assays in POSF presented a positive correlation with β-glucan content. Our study suggests that these co-products could have several food-related applications, such as potential for use as an emulsifier, sweetener, or fortifier in the development of functional food, owing to their rich concentrations of fibre, protein, sorbitol, and β-glucans. Nevertheless, it is necessary to understand the interactions of the flours with the potential food matrix prior to proceeding further with food-related applications.

Use of sawdust for production of ligninolytic enzymes by white-rot fungi and pharmaceutical removal.

Use of white-rot fungi for enzyme-based bioremediation of wastewater is of high interest. These fungi produce considerable amounts of extracellular ligninolytic enzymes during solid-state fermentation on lignocellulosic materials such as straw and sawdust. We used pure sawdust colonized by Pleurotus ostreatus, Trametes versicolor, and Ganoderma lucidum for extraction of ligninolytic enzymes in aqueous suspension. Crude enzyme suspensions of the three fungi, with laccase activity range 12-43 U/L and manganese peroxidase activity range 5-55 U/L, were evaluated for degradation of 11 selected pharmaceuticals spiked at environmentally relevant concentrations. Sulfamethoxazole was removed significantly in all treatments. The crude enzyme suspension from P. ostreatus achieved degradation of wider range of pharmaceuticals when the enzyme activity was increased. Brief homogenization of the colonized sawdust was also observed to be favorable, resulting in significant reductions after a short exposure of 5 min. The highest reduction was observed for sulfamethoxazole which was reduced by 84% compared to an autoclaved control without enzyme activity and for trimethoprim which was reduced by 60%. The compounds metoprolol, lidocaine, and venlafaxine were reduced by approximately 30% compared to the control. Overall, this study confirmed the potential of low-cost lignocellulosic material as a substrate for production of enzymes from white-rot fungi. However, monitoring over time in bioreactors revealed a rapid decrease in enzymatic ligninolytic activity.

Quantifying the potential renal acid load of edible mushrooms

The capability of any food to alter net endogenous acid or base production can be estimated using the potential renal acid load (PRAL) estimation method. The PRAL of edible mushrooms has been rarely examined; thus a quantification study of n = 37 edible mushroom species was performed. Results revealed a heterogeneous picture: although the most commonly consumed mushrooms (e.g., Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus (white)) have alkalizing properties, several acidifying species (Volvariella volvacea, Pleurotus flabellatus) were identified, which may be attributable to their high phosphorus content.

Advances in the Applications of Clinoptilolite-Rich Tuffs.

Adsorptive, catalytic, and antibacterial properties of clinoptilolite-rich tuffs (ZT) are presented here. ZT transformed into Fe-containing ZT (Fe-ZT) removes various organic and inorganic anions from water. Fe-ZT, which contains selenium, is beneficial for growing Pleurotus ostreatus mushrooms. The fungi convert inorganic Se from Fe-ZT into a more useful organically bonded form. ZT and Fe-ZT as supplements retain nitrogen and potassium in sandy, silty loam and silty clay soils. ZT shows an affinity toward toxic metal cations, which are essential for cleaning contaminated water. The adsorption of atenolol, acetylsalicylic, and salicylic acid onto M-ZT (M-Cu2+, Mn2+, Ni2+, or Zn2+) from water solutions suggests that both the natures of M and pharmaceuticals have a significant impact on the adsorption mechanism and determine the adsorption capability of the ZT. ZT is an excellent carrier for ultrafine (2-5 nm) nano oxide particles, which have been shown to have catalytic activity in different chemical processes and photodegradation reactions of organic pollutants. ZT can also be transformed into SO4-SnO2-ZT, which is catalytically active as a solid acid. M-ZT is an effective carrier of valuable bacteria. Ag-ZT possesses beneficial bactericidal activity in disinfecting water and soil remediation.

Enhanced production of mycelium biomass and exopolysaccharides of Pleurotus ostreatus by integrating response surface methodology and artificial neural network

This study aimed to enhance the production of mycelium biomass and exopolysaccharides (EPS) of Pleurotus ostreatus in submerged fermentation. Response Surface Methodology (RSM)sought to optimize culture conditions, whereas Artificial Neural Network (ANN)aimed to predict the mycelium biomass and EPS. After optimization of RSM model conditions, the maximum biomass (36.45 g/L) and EPS (6.72 g/L) were obtained at the optimum temperature of 22.9 °C, pH 5.6, and agitation of 138.9 rpm. Further, the Genetic Algorithm (GA) was employed to optimize the cultivation conditions in order to maximize the mycelium biomass and EPS production. The ANN model with an optimized network structure gave the coefficient of determination (R2) value of 0.99 and the least mean squared error of 1.9 for the validation set. In the end, a graphical user interface was developed to predict mycelium biomass and EPS production.

Emulsifying properties of β-glucan-rich extracts obtained from Pleurotus ostreatus whole mushroom and stipes

In this work, aqueous fractions were extracted from the whole Pleurotus ostreatus biomass (AF) and from a waste product (RAF) generated from it in the industry (stipes) with the aim of valorizing them as emulsifying ingredients. The composition of the aqueous extracts, before and after a purification step (p) carried out to enrich its content in β-glucans, was correlated with the physical stability, rheological properties and microstructure of oil-in-water (O/W) emulsions. Better emulsifying properties were obtained for AFp-based emulsions (being able to incorporate greater amounts of oil phase), than for emulsions formulated with non-purified fractions. These properties can be attributed to the relatively high protein content of AFp fractions (acting at the O/W interphase) and high viscosity of the continuous phase which slowed down the oil droplet movement. The lower emulsifying properties of β-glucan extracts obtained from the stipes was ascribed to the lower content of proteins, which were less accessible to act as surface-active compounds due to the greater glucan structural complexity with which they are interacting.

Exploring New Fruit- and Vegetable-Derived Rennet for Cheese Making

Cheese production is an ancient practice to preserve a perishable food, such as milk, for a long time. The first step of cheese processing involves the addition of rennet, which contains the enzymes necessary for the hydrolysis and coagulation of the caseins present in milk. Typically, animal-derived rennet, such as calf rennet containing chymosin, are used as source of enzymes for cheese processing. Alternatively, microbial chymosin or recombinant chymosin is used. However, recently, plant-derived rennet such as the ones derived from thistle and bitter orange flowers and from artichoke (Cynara cardunculus var. scolymus) have also been demonstrated to be valid sources of enzymes for cheese processing. Therefore, herein, different plant and fruit extracts were tested and compared for their ability to coagulate milk caseins. In particular, beyond artichoke and cardoon (Cynara cardunculus) extracts, those from pineapple (Ananas comosus (L.) Merr.), papaya (Carica papaya L.), common fig (Ficus carica L.) milky sap, and oyster mushroom (Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm.) were investigated for their proteolytic, esterase, and milk-clotting activities. The extracts were then exploited as vegetable and fruit rennet for the experimental production of cheeses, which were examined, after 30 days of maturation, for their moisture, fat, protein, and free fatty acid (FFA) content. Interestingly, the artichoke, cardoon, and thistle mushroom extracts showed high proteolytic activity compared to calf rennet, while the level of esterase activity appeared to be similar for all the extracts. The papaya extract showed the lowest proteolytic and esterase activity. Although the pH, moisture, fat, and protein contents were very similar to those of cheese made with calf rennet, the medium- and long-chain FFAs broadly differed among produced cheeses, with variations in the lipid quality indices.

Effects of Environmental and Nutritional Conditions on Mycelium Growth of Three Basidiomycota

In recent decades, an enormous potential of fungal-based products with characteristics equal to, or even outperforming, classic petroleum-derived products has been acknowledged. The production of these new materials uses mycelium, a root-like structure of fungi consisting of a mass of branching, thread-like hyphae. Optimizing the production of mycelium-based materials and fungal growth under technical conditions needs to be further investigated. The main objective of this study was to select fast-growing fungi and identify optimized incubation conditions to obtain a dense mycelium mat in a short time. Further, the influence of the initial substrate characteristics on hyphae expansion was determined. Fungal isolates of Ganoderma lucidum, Pleurotus ostreatus, and Trametes versicolor were cultivated for seven days on substrate mixtures consisting of various proportions of pine bark and cotton fibers. Furthermore, the substrates were mixed with 0, 2, and 5 wt.% calcium carbonate (CaCO3), and the incubator was flushed with 0, 5, and 10 vol.% carbon dioxide (CO2). All samples grew in the dark at 26 °C and a relative humidity of 80%. Evaluation of growth rate shows that cotton fiber-rich substrates performed best for all investigated fungi. Although Pleurotus ostreatus and Trametes versicolor showed comparatively high growth rates of up to 5.4 and 5.3 mm d−1, respectively, mycelium density was thin and transparent. Ganoderma lucidum showed a significantly denser mycelium at a maximum growth rate of 3.3 mm d−1 on a cotton fiber-rich substrate (75 wt.%) without CaCO3 but flushed with 5 vol.% CO2 during incubation.

Nameko Mushroom (Pholiota microspore), Hiratake Mushroom (Pleurotus ostreatus) and Shimeji Mushrooms (Lyophyllum shimeji): Bioactive Compounds as a Source of Various Biological Activities: A Review

Mushrooms are substantial source of nutritional, medicinal compounds, and other benefits compounds still need to be worked out for their important applications. Mushrooms as higher Basidiomycetes and Ascomycetes contain secondary metabolites in their fruit bodies, cultured mycelium, and cultured broth. These secondary metabolites have been used in many sides of human activity for many years for maintaining long and healthy life. The genera Pholiota, Pleurotus and Lyophyllum are a group of edible mushrooms that are distributed all over the world. The studies on different species of these edible mushrooms have revealed their nutritional medicinal potentials. This review aims to present the importance of Pholiota microspore, Pleurotus ostreatus and Lyophyllum shimeji, as both food and medicine, and they offer new visions to researchers to develop new drugs and nutraceuticals.

Identification of two genes essential for basidiospore formation during the postmeiotic stages in Pleurotus ostreatus

A sporeless strain is an important breeding target in the mushroom industry. However, basidiospore production in the oyster mushroom Pleurotus ostreatus has been shown to be impaired by single-gene mutations in only two meiosis-related genes, mer3 and msh4. This study proposed a strategy for identifying the genes essential for basidiospore formation after meiotic division to determine new targets for molecular breeding. RNA-seq analysis was performed to identify P. ostreatus genes that are specifically expressed in the gill tissue of fruiting bodies, where basidiospore formation occurs. Transcriptome data during fruiting development of Coprinopsis cinerea, in which the meiotic steps progress synchronously, were then used to identify genes that are active in the postmeiotic stages. Based on these comparative analyses, five P. ostreatus genes were identified. Plasmids containing expression cassettes for hygromycin B-resistance screening, Cas9, and single-guide RNA targeting each gene were introduced into the protoplasts of dikaryotic strain, PC9×#64, to generate dikaryotic gene disruptants. Among the obtained transformants, three dikaryotic pcl1 disruptants and two cro6c disruptants did not produce basidiospores. Microscopic analyses indicated that spore formation was arrested at particular stages in these gene disruptants. These results indicate that these two genes are essential for mature spore formation in this fungus.

Filamentous Fungi as Bioremediation Agents of Industrial Effluents: A Systematic Review

The industrial sector plays a significant role in global economic growth. However, it also produces polluting effluents that must be treated to prevent environmental damage and ensure the quality of life for future generations is not compromised. Various physical, chemical, and biological methods have been employed to treat industrial effluents. Filamentous fungi, in particular, have garnered attention as effective bioremediation agents due to their ability to produce enzymes capable of degrading recalcitrant compounds, and adsorb different pollutant molecules. The novelty of the work reported herein lies in its comprehensive assessment of the research surrounding the use of white- and brown-rot fungi for removing phenolic compounds from industrial effluents. This study employs a systematic review coupled with scientometric analysis to provide insights into the evolution of this technology over time. It scrutinizes geographical distribution, identifies research gaps and trends, and highlights the most studied fungal species and their applications. A systematic review of 464 publications from 1945 to 2023 assessed the use of these fungi in removing phenolic compounds from industrial effluents. White-rot fungi were predominant (96.3%), notably Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, and Lentinula edodes. The cultures employing free cells (64.15%) stand out over those using immobilized cells, just like cultures with isolated fungi regarding systems with microbial consortia. Geographically, Italy, Spain, Greece, India, and Brazil emerged as the most prominent countries in publications related to this area during the evaluated period.

Edible colorimetric label based on immobilized purple sweet potato anthocyanins onto edible film for packaged mushrooms freshness monitoring.

An edible colorimetric label has been developed to determine the freshness level of mushrooms, i.e. white oyster mushrooms (Pleurotus ostreatus). The edible indicator label has been fabricated based on purple sweet potato (Ipomoea batatas L.) anthocyanins (PSPA) immobilized onto an edible film made of chitosan and cornstarch with added PVA. The freshness parameters of the mushrooms were pH, weight loss, texture, and sensory evaluation. The results showed that the colorimetric label was dark purple when the mushroom was fresh, and turn to light purple when the mushroom was still fresh, and finally green when the mushroom was no longer fresh. The color value (mean Red) of the label was measured using the ImageJ program, where its color value (mean Red) increased with decreasing freshness level of the mushrooms. The edible label can distinguish fresh mushrooms from spoilage, making it suitable to be used in a packaged mushroom as a freshness indicator.

Assessment of the effect of iron and selenium fortification on the amino acids profile of Pleurotus ostreatus

Assessment of the effect of iron and selenium fortification on the amino acids profile of Pleurotus ostreatus

Elemental Composition of A Cultivated Mushroom (Agaricus bisporus) and Some Wild Mushroom Species

In the study, a cultivation mushroom (Agaricus bisporus) and some wild mushroom species (Schizophyllum commune, Pleurotus ostreatus, Lactarius deliciosus, Hebeloma sinapizans, Hygrophorus ligatus, Suillus luteus, Armillaria mellea, Coprinus comatus, Psathyrella candolleana, Russula torulosa, Trametes pubescens) were investigated in terms of elemental compositions. The wild mushroom species were collected from Trabzon province. Mg, Al, Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Rb, Sr, Y, Zr, I, Hf, Hg, Pb metal concentrations were determined using Energy Dispersive X-ray Fluorescence (ED-XRF) device. The highest aliminium, silicium, vanadium, zirconium, iron and mercury were determined in Lactarius deliciosus. The highest potassium, arsenic, rubidium, iodine contents were determined in Hebeloma sinapizans. The highest manganese, zinc, lead contents were determined in Hygrophorus ligatus. The highest magnesium, chromium, nickel, yttrium contents were determined in Coprinus comatus. The highest titanium and selenium were determined in Psathyrella candolleana. The highest calcium and strontium were determined in Russula torulosa. The highest hafnium was determined in Schizophyllum commune. The highest phosphate was determined in Agaricus bisporus. The highest sulphur was determined in Armilleria mella. The highest copper was determined in Suillus lutesus. It was concluded that elemental composition of mushrooms was affected especially by mushroom specie.

Environmental implications of three Pleurotus strain growths for water remediation in the perspective of climate change in New Egyptian Delta

Recently, the integrated different interdisciplinary studies derived the environmental solutions of the climate change impacts (e.g., cultivation, wastewater treatment, and managing groundwater resources) (Mesalhy et al. 2020, and Gobashy et al. 2021). Thus, this paper focused on the application of bioremediation to maximize the use of wastewater for new reclamation areas in the Northwest Egyptian desert (New Egyptian Delta (NED). In the NED project, the drainage water samples collected from Nile Delta drains will provide the main unconventional water resources for irrigation through the new Hammam canal. Therefore, three Pleurotus strains were grown moderately on two natural media, the first containing Salvia L. (sage) extract (MDA) and the second containing Thymus vulgaris L. (origanum thymus Kuntze, Thymus collinus Salisb) (TDA) extract replacing potato infusions in standard PDA. Pleurotus ostreatus (Jacquin; Kummer) strain records the highest growth among the three tested fungi on modified media. PO records 4.49 and 4.41 cm on (MDA) and (TDA), respectively. There is a marked decrease in the majority of heavy metal concentrations on sterile drainage water amended with PD broth and inoculated with three tested Pleurotus strains individually. At the end of the incubation period, Pleurotus ostereatus which expressed in abbreviation (PO) are more efficient in the removal of Al, Co, Cr, and Ni by 53.15, 95.87, 58.47, and 85.07%; respectively. Pleurorotus pulmonarius (Fr.) which symbolized (PP) is more potent in the removal of Cd, Si, Sn, Sr, and V by 70.37, 56.59, 41.19, 52.78, and 96.24%; respectively. Pleurotus floridanus (NZOR) which indicated as (PF) is actively over the former species in the removal of Ba, Fe, and Mo by 87.84, 46.67, and 97.34%; respectively. Cu, Mn, Pb, As, and Se could not be detected as the control sample recorded measurements below 0.009 mg L−1. An unexpected increase in Zn among the different treatments was detected from 05.04 to 07.01%.

Physico-Chemical, Sensory, and Nutritional Properties of Shortbread Cookies Enriched with Agaricus bisporus and Pleurotus ostreatus Powders

Mushrooms, due to their basic composition and the presence of numerous mycochemicals, can be used to improve various food matrices. The objective of this study was to determine the impact of replacing wheat flour (2%, 4%, 6% w/w) with mushroom lyophilisates from cultivated mushrooms—A. bisporus and P. ostreatus—on the technological quality, basic nutritional and elemental composition, antioxidant activity (ABTS, FRAP), total polyphenol content (TPC), and sensory evaluation of shortbread cookies. The functional properties of blended flours were also determined, such as bulk density (BD), water- and oil-holding capacity (WHC, OHC), swelling capacity (SW), and water solubility index (WSI). The results show that the amounts of protein, fiber, and ash were higher in cookies enriched with mushrooms than in control cookies. The enriched products, depending on the amount of mushroom powder used and the mushroom species, had a higher content of zinc, iron, magnesium, potassium, and copper. The increase in the addition of mushroom powder resulted in a significant (p &lt; 0.05) increase in the TPC content and antioxidant properties. The use of composite flours contributed to a significant increase in hardness (at 6% mushroom powder) and a change in color parameters, with lower whiteness and a greater ΔE recorded for cookies with A. bisporus lyophilisate. In the sensory evaluation, the samples enriched with P. ostreatus powder received higher scores compared with control samples, while the cookies with A. bisporus flour were evaluated lower than the control.

Exploring Therapeutic Potential of Pleurotus ostreatus and Agaricus bisporus Mushrooms against Hyperlipidemia and Oxidative Stress Using Animal Model.

The mushrooms oyster (Pleurotus ostreatus) and white button (Agaricus bisporus) contain bioactive compounds that have potential beneficial effects on hypercholesterolemia and cardiovascular diseases. In this study, hypolipidemic and antioxidative potential of these mushrooms' extract were explored using hypercholesterolemic (HC) rats as animal model. For the study, 56 adult rats were divided into seven groups, i.e., G1 (negative control), G2 (positive control group), G3 (HC rats with statin drug orally), G4 and G5 (HC rats @ 100 and 200 mg/kg body weight (BW) dose of oyster mushroom extracts), and G6 and G7 (HC rats @ 100 and 200 mg/kg BW dose of white button mushroom extracts). The hypercholesterolemia was induced experimentally in fasted rats through a high-fat diet along with injection of triton WR-1339. After 48 h, the treatment groups were given extract for 28 days along with standard diet. At the trial termination, we analyzed the blood sugar levels, antioxidant parameters, lipid profile, and renal function, as well as conducting liver function tests of the rats. The results indicated that positive control group rats exhibited increased levels of total cholesterol (TC), triglycerides (TG), low-density level (LDL), and very-low-density level (vLDL) by 19%, 37%, 52%, and 32%, respectively, and 53% decrease in HDL, whereas treatment groups that received 200 mg oyster and white button mushroom extracts reported 15%, 34%, 22% reduction in TC, TG, vLDL, respectively, and 22% improvement in HDL level. The enzyme profiles of different groups showed non-significant differences, although both mushroom extracts provision reduced glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels. Overall, the results indicated that mushroom extracts were helpful in maintaining oxidative stress and have the potential to improve dyslipidemia in the tested rat animal model.