Granulomatous Synovitis Research Articles

Granulomatous Synovitis Caused by a Mycobacterial Avium-Intracellulare Complex.

Granulomatous Synovitis Caused by a Mycobacterial Avium-Intracellulare Complex.

P152 African Woman With a Knee Knocked by a Rare Dematiaceous Fungi

Poster session 2, September 22, 2022, 12:30 PM - 1:30 PMObjectives/IntroductionIncidence of fungal peri-prosthetic joint infection (PJI) is rare (1%-3%) and the majority are caused by Candida and Aspergillus. We report a peri-prosthetic knee joint infection caused by a rare dematiaceous fungi—pleurostomophora richardsiae, probably the first case in the world.Methods/Case DetailsA 78-year-old East African female from Malawi, housewife, with no known medical comorbidities presented with a chronic history of left knee pain and pus discharge. She had left knee pain since 2008 and was given several intra-articular injections between 2008 and 2010 for pain relief suspecting osteoarthritis. She denied a history of splinter injuries, trauma, systemic, or constitutional symptoms.In 2010 she underwent left total knee replacement (TKR) in the USA, but the pain persisted post-operatively associated with intermittent swelling of both knees. She was evaluated again in 2015 and revision left TKR done with single stage exchange.She was asymptomatic for a few years but symptoms worsened again and drainage of pus from her left knee started in December 2019. She was treated in Malawi with multiple courses of parenteral and oral antibiotics but did not improve.She presented to our hospital in January 2022 with swelling in left knee and restriction of movements. On examination, a discharging sinus was noted over the medial aspect of left knee. She was anemic with a normal leucocyte count, HIV negative, ESR of 85 mm/h, and CRP of 23 mg/L. Her renal and liver function tests were normal. CT left leg with sinogram showed features of chronic osteomyelitis of left distal femur and proximal tibia with active sinus tract in left tibia.Sinus tract excision with removal of prosthesis, debridement, and antibiotic cement spacer insertion was done. Bone and peri-prosthetic tissue were sent for histopathology and microbiological analysis including fungal and mycobacterial cultures. Xpert MTb was negative.Histopathology showed granulomatous synovitis with fungal hyphae and spores. Cultures grew a slender septate dark pigmented fungus, Pleurostomophora richardsiae which was confirmed by fungal PCR sequencing of internal transcribed spacer (ITS) region.Results/TreatmentShe was treated with Liposomal amphotericin B 5 mg/kg IV OD for 2 weeks followed by oral Itraconazole. She had persistent raised inflammatory markers at 4 weeks which settled after changing to posaconazole for 2 weeks. Conservative management will continue for 3-6 months with second stage revision arthroplasty/arthrodesis later.ConclusionDematiaceous fungi usually cause skin and soft tissue infections and they are extremely rare in causing prosthetic joint infection. Case reports of P. richardsiae causing osteomyelitis of foot and endocarditis are available but we couldn't find a published case of prosthetic joint infection caused by it. Identifying the causative organism in PJI is the most important step because the management depends mainly on it.Two-stage exchange in combination with antifungal administration between stages and post-revision should be the procedure of choice for fungal PJI. Incorporation of antifungal agents into cement spacers appears to be effective in eradicating local infections and reducing the duration of antifungal treatment and should be strongly considered.

An unusual clinical presentation of granulomatous tophaceous gout in the wrist

The possibility of infection with mycobacterium tuberculosis (TB), and other infectious agents is the first option when granulomatous inflammation wasobserved in the histological examination of a tissue specimen. However, gout with manifestations of granulomatous synovitis may mimic TB tenosynovitis. An 80-year-old male with a history of hypertension, and gout presented with the swelling of the right wrist, and deformity as a result of trauma. The results of cytology for malignancy, TB polymerase chain reaction (PCR), and TB culture were negative. Histopathological examination with an electron microscope confirmed the diagnosis of tophaceous gout along with granulomatous synovitis. The current case emphasizes the significance of a correct gouty process diagnosis in patients who exhibit granulomatous tophaceous tuberculous tenosynovitis or rheumatoid arthritis. We suggested that gout should be considered in differential diagnosis of granulomatous synovitis, especially in cases with negative mycobacterium TB-PCR.

Metal Artefact Reduction Sequences (MARS) in Magnetic Resonance Imaging (MRI) after Total Hip Arthroplasty (THA)

BackgroundIn the past, radiographic imaging was of minor relevance in the diagnosis of periprosthetic joint infections (PJI). Since metal artefact reduction sequences (MARS) are available, magnetic resonance imaging (MRI) has become a promising diagnostic tool for the evaluation of hip arthroplasty implants. The purpose of the present study was to evaluate the efficacy of MARS-MRI in comparison to established diagnostic tools to distinguish between aseptic failure and PJI.MethodsFrom July 2018 to September 2019, 33 patients classified as having an aseptic joint effusion were recruited into the study. The group included 22 women and 11 men with a mean age of 70.4 ± 13.7 (42–88) years. In the same period, 12 patients were classified as having a PJI. The group consisted of 9 women and 3 men with a mean age of 72.5 ± 10.6 (54–88) years. MARS-MRI was conducted using the optimized parameters at 1.5 T in a coronal and axial STIR (short-tau-inversion recovery), a non-fat-saturated T2 in coronal view and a non-fat-saturated T1 in transverse view in 45 patients with painful hip after total hip arthroplasty (THA). Normally distributed continuous data were shown as mean ± standard deviation (SD) and compared using student's t-test. Non-normally distributed continuous data were shown as mean and compared using the Mann–Whitney U test.ResultsSynovial layering and muscle edema were significant features of periprosthetic joint infection, with sensitivities of 100% and specifities of 63.0—75.0%. The combined specifity and sensitivity levels of synovial layering and muscular edema was 88.0% and 90.0%. Granulomatous synovitis was a significant feature for aseptic failure, with 90.0% sensitivity and 57.0% specifity.ConclusionMARS-MRI is as suitable as standard diagnostic tools to distinguish between aseptic failure and PJI in patients with THA. Further studies with larger patient numbers have to prove whether MARS-MRI could be integral part of PJI diagnostic.

Synoviale Veränderungen bei Erkrankungen des rheumatologischen Formenkreises und Differenzialdiagnosen

Die Untersuchung synovialer Veränderungen kann zur Diagnose von Gelenkerkrankungen und von systemischen Erkrankungen beitragen. Die Domäne der histopathologischen Diagnostik stellt die Abgrenzung tumoröser von entzündlichen Läsionen dar. Daneben können Kristallarthropathien und bestimmte Stoffwechselerkrankungen sicher diagnostiziert werden. Unter dem histologischen Bild einer granulomatösen Synovialitis können neben einer mykobakteriellen Infektion Sarkoidosen und Fremdkörperreaktionen sowie selten genetische Erkrankungen beobachtet werden. Amyloidosen können auch in der Tunica synovialis subtypisiert werden. Molekulare Methoden erlauben die schnelle und sichere Diagnostik septischer Arthritiden und eine Keimtypisierung. Mittels dieser Methoden können auch reaktive Arthritiden klassifiziert werden, da auch hier häufig DNA oder RNA bestimmter Keime in Gewebe oder Gelenkflüssigkeit nachgewiesen werden kann. Die Diagnose der rheumatoiden Arthritis basiert auf den American College of Rheumatology(ACR)-Kriterien. Molekulare Methoden, wie die Mikro-RNA-Technologie oder proteomische Methoden können die Diagnose unterstützen.

Granulomatous lesions !! Expected guests in unexpected places

Introduction: Granulomatous lesions in various sites have different modes of presentation, different etiologic factors with identical histological patterns. The aim was to study the occurrence of granulomatous lesions at uncommon sites. Materials and methods: A retrospective study of histopathological specimens received over a period of one year from June 2017 to May 2018 was done and cases of granulomatous lesions of various sites reported on histopathological examination were reviewed along with Ziehl-Neelsen (ZN) and Fite-Faraco (FF) staining. Results: Out of total 3623 histopathology specimens, 61 (1.7%) were granulomatous lesions of which 28 (45.9%) were AFB (Acid-fast bacilli) positive. 20 cases (32.7%) were granulomatous lymphadenitis out of which 12 (19.6%) were positive for AFB. 4 (6.5%) out of 13 (21.3%) granulomatous skin lesions showed AFB positivity. 6 (9.8%) cases of granulomatous mastitis (1 was AFB +ve), 4(6.5%) cases of granulomatous synovitis (2 were AFB +ve), 3 (4.9%) cases of granulomatous endometritis (2 were AFB+ve) were seen. 2 (3.3%) cases each of granulomatous epididymitis and appendicitis were noted. 1(1.6%) case of HIV positive hysterectomy specimen showed positivity for AFB. 1 (1.6%) case each of granulomatous lesions was found in the salivary gland, thyroid, testis, parietal pleura, nasal septum, suture granuloma of the fallopian tube, lipoma with foreign body granuloma, sinus tract, intestine, and omentum. Conclusion: In the present study tuberculosis was the most common cause of granulomatous lesions of various sites. Histopathology plays an important role in the diagnosis and management of a variety of granulomatous lesions. Special stains play a vital role in the diagnosis of infectious granulomatous lesions.

Mycobacterial Arthritis and Synovitis in Painted Reed Frogs (Hyperolius marmoratus)

Several species of atypical mycobacteria have been isolated from wild and captive amphibians. In captive anurans, cutaneous and visceral mycobacteriosis are common and can result in significant mortality, particularly when animals are immunocompromised. Mycobacterial arthritis and synovitis are reported rarely in amphibians. We describe 20 cases in painted reed frogs (Hyperolius marmoratus), which presented with cachexia, limb paresis or paralysis or 'spindly leg syndrome'. Histopathology revealed multifocal histiocytic to granulomatous synovitis affecting appendicular, rib or spinal intervertebral joints. Periarticular granulomata, granulomatous cellulitis and skeletal muscle atrophy, necrosis and degeneration were also present. In one case, granulomatous spinal osteomyelitis was recorded. Ziehl-Neelsen stains showed large numbers of acid-fast bacteria in macrophages and histiocytes. The mycobacterial isolates obtained from culture were identified as members of the Mycobacterium chelonae complex (either M. chelonae or Mycobacterium abscessus). This was confirmed by 5'-16S ribosomal ribonucleic acid (rRNA) sequencing. In 17 cases mycobacterial lesions were present only in the joints and skeleton, highlighting the importance of not ruling out mycobacterial infection on the basis of absence of cutaneous or visceral lesions.

Total Knee Arthroplasty in a Case of Tuberculosis Knee in Healing Stage: Is it Safe?

ABSTRACT Background Association of tuberculosis and total hip joint replacement (THR) is described with or without antitubercular treatment (ATT) cover but total knee arthroplasty (TKA) in early disease in single-stage is uncommonly reported. We wish to share our clinical experience in such a case. Case description A 22-year-old male presented with pain and swelling of left knee for 7 months. It was drained at local health facility with sinus development. Tuberculosis was diagnosed by clinic-radiological evaluation and ATT was started. Sinus healed but disabling pain and knee stiffness with flexion deformity persisted. Radiographs revealed destructive osteoarticular arthritis with periarticular osteopenia. Debridement followed by TKA in single-stage was done with posterior-stabilized implant. Postoperative period was uneventful. Histopathological analysis confirmed chronic granulomatous synovitis with caseation necrosis suggestive of mycobacterial infection. After 1-year, ATT was discontinued and patient was disease-free. Clinical relevance Single-stage prosthetic knee joint arthroplasty can be safely performed under adequate ATT coverage in early disease provided response to medical treatment is good. Patient counseling regarding compliance with ATT and postoperative protocol is important in ensuring success. How to cite this article Yadav S, Yadav CS, Kumar N, Kumar A. Total Knee Arthroplasty in a Case of Tuberculosis Knee in Healing Stage: Is it Safe? J Postgrad Med Edu Res 2015;49(3): 139-142.

Molecular pathological diagnostics of infections in orthopedic pathology

The diagnosis of infections in patients with arthritis and/or in joint prostheses requires interdisciplinary cooperation and the application of up-to-date methods. The histological investigation of the synovial membrane allows the differentiation of acute, chronic and granulomatous synovialitis. Detection of conserved regions of the microbial genome by PCR, especially 16S rRNA for bacteria and 18S rRNA for fungi, is a broad approach for the classification of pathogens which cannot be cultured. Acute infectious arthritis and periprosthetic infections share the spectrum of pathogens with sepsis, therefore multiplex PCR-based methods for the detection of sepsis can be employed. Molecular diagnostics can detect minimal infections in periprosthetic tissues even after antibiotic therapy. The anamnesis (enteral or urogenital infection), clinical picture (oligoarthritis) and further parameters (e.g. HLA B27 status) are important for the diagnosis of reactive arthritis. In many cases of reactive arthritis, molecular methods allow the detection of bacterial DNA or RNA in synovial fluid or tissue samples. The low sensitivity of histopathological methods may be compensated by application of PCR techniques, especially in the differential diagnosis of granulomatous synovitis including mycobacterial infections. Molecular methods can be used to support the differential diagnosis of septic and reactive arthritis. MicroRNA techniques combined with PCR for detection of pathogens support the differential diagnosis of rheumatoid arthritis with severe inflammatory activity compared to infectious arthritis. Proteomic methods could expand the methodological spectrum for the diagnosis of infections.

Granulomatous synovitis of wrist in rheumatoid arthritis- is it tuberculosis of wrist?

Granulomatous synovitis of wrist in rheumatoid arthritis- is it tuberculosis of wrist?

AB0971 New Availability of an Old Modality; Useful Grading System for Gout Tophi on Plain Radiography

BackgroundGout is caused by hyperuricemia related to altered purine metabolism characterized by monosodium urate (MSU) crystals deposition into the joints, particularly the first metartarsophalangeal joint (1st MTPJ). Chronic gout is...

Bilateral Hip Joint Hylan G-F 20 Granulomatous Synovitis due to Viscosupplementation Injections.

We present the diagnosis of bilateral granulomatous inflammation of the hip joints associated with Hylan G-F 20 viscosupplementation injections. Clinicians recommending therapeutic Hylan injections for the management of hip arthritis should maintain clinical awareness regarding this potential complication.

HISTOPLASMOSE ARTICULAR: RELATO DE CASO DE SINOVITE GRANULOMATOSA

We report a case of sub acute granulomatous synovitis of the knee due to Histoplasma capsulatum in a patient with a past history of intra-articular corticosteroid injections for right knee pain. The diagnosis of histoplasmosis was made on microscopical examination of the surgical specimen. Subsequent cultures from the synovial fluid grew H. capsulatum. This is an unusual case of focal histoplasmosis. This diagnosis should be considered in endemic areas.

Multimodality imaging of chronic tophaceous gout

The diagnosis of gout is usually based on clinical presentation and laboratory findings. Imaging plays a role in the assessment and grading of articular damage related to chronic, long-standing disease, which is characterized by granulomatous synovitis, tophi, and erosions. Multimodality imaging of chronic tophaceous gout may be useful in clinical practice for a variety of purposes, including assessment of disease-related anatomical changes and monitoring of articular and soft-tissue lesions over time, especially in response to urate-lowering therapy. Radiography remains the primary imaging technique. Ultrasonography may detect monosodium urate crystals on cartilage, is helpful to assess small joint effusion, to guide to joint aspiration, and to evaluate the volume of tophi. Computed tomography is considered to be more sensitive than plain radiography in the detection and evaluation of cortical bone erosions associated with tophi. MRI represents the only imaging modality which provides visualization of bone marrow oedema associated with erosions and may be useful to characterize and distinguish tophi from other soft tissue nodules.

Rezidivierende Ergussbildung und granulomatöse Synovitis nach Knietotalendoprothesenimplantation bei Latexallergie

An increasing number of articles report about allergic reactions in association with total knee arthroplasty. While most studies focus on allergic reactions to metallic components, few reports exist about reactions to bone cement or its ingredients. Allergy to natural rubber latex is a major occupational problem in the health care sector and a problem even in other occupations in which protective gloves are used. The allergic reaction to latex ranges from a minor skin rash to anaphylactic shock. Preventing exposure to latex is the key to managing and preventing this allergy. We report about a patient who developed recurrent effusion and granulomatous synovitis following total knee arthroplasty in association with latex allergy.

Polyarticular arthritis secondary to Mycobacterium bovis infection: An unusual clinical presentation

We describe a 37-year-old Mexican man with incapacitating polyarticular arthritis secondary to Mycobacterium bovis infection. A dermatologist diagnosed psoriasis two years before admission. One year later due to symmetric ankle and knee arthritis, he was treated with three doses of etanercept. The arthritis extended to the carpus and metacarpophalangeal joints. Multifocal dactylitis and a left ankle periarticular abscess were documented. Concomitantly he developed fever, cough, and adenopathies. Ankle MRI showed osteomyelitis of the calcaneous with a posterior abscess. A CT-body scan documented mild pleural effusion that corresponded to an exudate. An ankle aspiration yielded a caseous fluid with acid-fast bacilli. Knee and ankle synovial biopsies documented a granulomatous synovitis. A lymph node biopsy showed granulomas with caseous necrosis. After 1 month, Mycobacterium bovis was isolated from these tissues. Antituberculous regimen was started with satisfactory response. Although largely eradicated, bovine tuberculosis still occurs. Nevertheless, the clinical presentation as polyarthritis is very uncommon and represents a diagnostic challenge.

Harmful Waste Products as Novel Immune Modulators for Treating Inflammatory Arthritis?

For many years, reactive oxygen species (ROS), including superoxide, hydrogen peroxide, and hydroxyl radicals, and their reaction products, were classically described as harmful by-products of aerobic metabolism capable of causing DNA mutations, lipid peroxidation, and protein oxidation [1]. The identifi cation of enzymes such as superoxide dismutase, catalase, and peroxidase that served to eliminate these waste products rather substantiated this view. It soon became clear, however, that there existed a family of enzymes whose function it was to deliberately generate ROS. The fi rst of these was NADPH oxidase, which is responsible for the respiratory burst in neutrophils and macrophages in response to microbes or infl ammatory cytokines [2]. The catalytic centre of NADPH oxidase is the membrane-associated protein gp91phox (see Glossary) complexed with p22phox. Activation requires association with a phosphorylated form of p47phox (also known as Ncf1), p67phox, and the small GTPase Rac (Figure 1). p47phox defi ciency in humans leads to neutrophil dysfunction and chronic granulomatous disease (CGD), a primary immunodefi ciency disorder characterised by the inability to eradicate bacterial infections [3]. Since the late 1990s, six human gp91phox homologues have been identifi ed, each with distinct functions [1]. Besides a role in phagocyte function and host defence, a large amount of evidence points to important roles for ROS in cell proliferation, apoptosis, angiogenesis, endocrinerelated functions, and oxidative modifi cation of the extracellular matrix. Indeed, increased ROS have been documented at sites of infl ammation, such as synovial joints of patients with infl ammatory arthritis (e.g., rheumatoid arthritis [RA]), and circulating neutrophils and monocytes from patients with RA have increased NADPH oxidase activity [4,5]. Interestingly, polyarthritis has also been described in patients with CGD. More recently, induction of arthritis in mice defi cient for the p47phox subunit of NADPH oxidase was shown to induce granulomatous synovitis and exaggerated matrix destruction associated with enhanced expression of infl ammatory mediators [6]. Although the mechanism for this paradoxical relationship between defi cient NADPH Harmful Waste Products as Novel Immune Modulators for Treating Infl ammatory Arthritis?

Septic arthritis and granulomatous synovitis caused by infection with Mycobacterium avium complex in a horse

A 12-year-old American Saddlebred gelding was referred to a veterinary teaching hospital for evaluation of a chronic lameness problem in the right radiocarpal joint. The horse had been treated for osteoarthritis of the right radiocarpal joint with multiple injections of cortisone during the past 3 years. The horse was severely lame on the right forelimb at a trot. Radiography and computed tomography revealed a 3 x 2-cm lytic defect in the distal portion of the radius and periarticular bone proliferation around the right radiocarpal joint. Ultrasonography of the distal portion of the radius revealed a soft tissue mass in the palmarolateral aspect of the joint. Proliferative synovium with a large amount of fibrin was observed in the dorsal and palmar aspects of the joint via arthroscopic examination of the right radiocarpal joint. Histologic examination of synovial biopsy specimens revealed proliferative granulomatous synovitis with giant cells. Mycobacterium avium complex was cultured from the synovial fluid. Infection with M avium complex should be considered in horses with chronic recurring arthritis associated with granulomatous synovitis.

Human parvovirus B19 transgenic mice become susceptible to polyarthritis.

Human parvovirus B19 (B19) often causes acute polyarthritis in adults. In this paper, we analyzed nucleotide sequences of the B19 genome of patients with rheumatoid arthritis (RA), and then introduced the nonstructural protein 1 (NS1) gene of B19 into C57BL/6 mice that had a genetic origin not susceptible to arthritis. The transgenic mice developed no lesions spontaneously, but were susceptible to type II collagen (CII)-induced arthritis. B19 NS1 was expressed in synovial cells on the articular lesions that were histologically characteristic of granulomatous synovitis and pannus formation in cartilage and bone. Serum levels of anti-CII Abs and TNF-alpha increased in NS1 transgenic mice to the same levels as those of DBA/1 mice, which were susceptible to polyarthritis. Stimulation with CII increased secretion of Th1-type- and Th2-type cytokines in NS1 transgenic mice, indicating that a nonpermissive H-2(b) haplotype in the wild type of C57BL/6 mice can be made susceptible to polyarthritis through the expression of NS1. This study is the first to show that a viral agent from the joints in humans can cause CII-induced arthritis resembling RA.

Synovite granulomateuse après injection de hylane GF-20. À propos de deux cas

Intra-articular hyaluronan injections are used to treat osteo-arthritis of the knee. Acute painful swelling with a joint effusion develops locally after the injection in about 10% of cases but resolves spontaneously. Crystals are identifiable in some patients, but the mechanism in crystal-negative cases remains unknown. We report knee arthritis with inflammatory joint fluid free of organisms and crystals in two patients after Hylan GF-20 treatment for femorotibial osteo-arthritis. Synovial membrane histology disclosed granulomatous synovitis with epithelioid histiocytic and multinucleate giant cells but no visible foreign bodies. These two cases suggest that crystal-negative arthritis after Hylan GF-20 injection may be ascribable to granulomatous synovitis of the foreign-body giant-cell type.