Gluten In Grain Research Articles

Wpływ diet eliminacyjnych na jakość życia osób z wybranymi chorobami autoimmunologicznymi

Złożoność etiopatogenezy schorzeń autoimmunologicznych, w tym celiakii i choroby Hashimoto oraz brak skutecznej farmakoterapii powoduje, że istotnym czynnikiem poprawy stanu zdrowia i komfortu życia oraz zmniejszenia rozwoju powikłań może być odpowiednia dietoterapia. W badaniach własnych zaobserwowano, że jedna trzecia osób z celiakią oprócz wyłączenia zbóż glutenowych eliminowała z diety produkty mleczne. Większość osób z chorobą Hashimoto wyłączyła z diety zboża glutenowe, a ponad połowa również produkty mleczne i soję. Największą poprawę stanu zdrowia, w tym szczególnie zmniejszenie występowania nudności, wzdęć, dolegliwości bólowych oraz zwiększenie uczucia komfortu obserwowały osoby z celiakią eliminujące oprócz zbóż glutenowych: mleko i jego przetwory, orzechy i soję. Podobnie największe zmiany samopoczucia związanego z objawami choroby Hashimoto, w tym szczególnie zmniejszenie nadwrażliwości na temperaturę, uczucia suchości skóry oraz poprawę samopoczucia deklarowały osoby eliminujące zboża glutenowe, produkty mleczne, orzechy arachidowe i warzywa psiankowate. Leczenie celiakii oparte na diecie bezglutenowej połączone z eliminacją orzechów i soi może przynieść największe korzyści związane z poprawą stanu zdrowia i jakości życia, a eliminacja zbóż glutenowych i orzeszków ziemnych może wspomagać poprawę jakości życia osób z chorobą Hashimoto.
 Słowa kluczowe: dieta eliminacyjna, Hashimoto, celiakia

THE STUDY RESULTS OF PRODUCTIVITY AND GRAIN QUALITY OF THE BREEDING LINES OF WINTER SOFT WHEAT IN THE COMPETITIVE VARIETY-TESTING

The article presents the study results of winter soft wheat lines in competitive variety testing according to productivity and grain quality indicators. The study was conducted at the experimental plot of the laboratory of the breeding and seed-growing of winter soft wheat of intensive type in the FSBSI “Agricultural Research Center “Donskoy” in 2016–2018. 47 winter soft wheat samples of competitive variety testing were used as the studied material. The winter soft wheat variety “Ermak” was used as a standard. Over the years of study 23 lines exceeded productivity of the standard variety “Ermak”, the increase ranged from 0.49 to 1.66 t / ha on average. The maximum protein percentage in grain was formed by the samples “1992/16” (13.5%), “1221/16” (13.5%), “1914/16” (13.6%), “1788/16” (13.9%), “2030/16” (14.0%). The lines “1854/16” and “1990/16” combining high yields (11.17–11.30 t/ha) with protein percentage of 12.9–13.2% are of great interest for breeding. The highest content of gluten in the grain (29.3–30.0%) was identified in the samples “1992/16”, “1914/16”, “2030/16”, “1221/16” and “1093/16”. There were identified the lines “1385/16”, “1533/16” and “1862/16”, combining high yields (11.20–11.59 t/ha) and 27.9–29.1% of gluten in grain. The highest value of SDS-sedimentation (66–70 ml) was found in the samples “1093/16”, “1990/16”, “2079/16”, “1533/16” and “1987/16”. The lines “1547/16”, “1385/16”, “1854/16” combined high yields (11.10–11.31 t/ha) with high value of SDS-sedimentation (62–65 ml). The breeding material identified during the research was used for further study and has been involved in hybridization to increase productivity and grain quality.

Productive and biochemical responses of durum wheat to UV filtration

AbstractA UV exclusion experiment was conducted on durum wheat (Triticum durum Desf. var. Claudio) grown in pots. Plants were grown under three different radiative treatments in greenhouses covered with plastic filters: Teflon, transparent to the entire region of natural UV‐visible sunlight (TEF); polyester, transparent above 312 nm (MYL, excluding UVB) and Lee, transparent above 400 nm (LEE, excluding both UVA and UVB). Analyses have been carried out to determine the concentration of photosynthetic pigments in leaves, UV‐absorbing compounds, nitrogen and carbon in leaves, culms and spikes and proteins and gluten in grains. In particular, plants grown under UV exclusion showed a reduction in protein and dry gluten content (consequently without variation in the ratio dry gluten/protein), but at the same time, a significant increase of gluten index, which is a parameter to define the quality of gluten, was observed. The results highlighted the influence played by UV radiation on some biochemical parameters, mainly UV‐absorbing compounds, leaf nitrogen and grain protein characteristics of durum wheat cultivated under Mediterranean conditions. In particular, natural level of UV in the Mediterranean improves the characteristics of durum wheat flour important for pasta production (high dry gluten level), while the UV exclusion could improve characteristics of flour important for bread production (high gluten index).

Залежність якості зерна пшениці озимої від погодних умов та систем удобрення у Південному Степу України

У статті відображено результати досліджень, отримані у тривалому польовому стаціонарному досліді упродовж 46 років. Метою досліджень було визначення якості зерна пшениці озимої за різних погодних умов та систем удобрення у Південному Степу України. Дослідження виконували у тривалому польовому стаціонарному досліді відділу агрохімії та родючості ґрунтів Одеської державної сільськогосподарської дослідної станції НААН на чорноземі південному малогумусному важкосуглинковому на лесовій породі, закладеному у 1971 р. Аналіз результатів тривалого польового стаціонарного досліду забезпечив отримання об’єктивних даних впливу погодних умов та дії добрив на якість зерна пшениці озимої упродовж шести ротацій сівозмін. Встановлено, що тривале використання на чорноземах південних органо-мінеральної системи удобрення забезпечувало кращу якість зерна пшениці озимої, найвищий вм іст білка і клейковини у якому відповідав вимогам другого класу. Відмічено, що показники якості зерна пшениці озимої залежали від особливостей погодних умов упродовж вегетаційного періоду. Достатня кількість тепла і вологи сприяла кращому засвоєнню азоту і нагромадженню білка та клейковини в зерні пшениці озимої із застосуванням всіх систем удобрення.

Mineral Composition and Baking Value of the Winter Wheat Grain under Varied Environmental and Agronomic Conditions

The mineral composition of cereal crops, the technological value of grain and flour, as well as bread quality are affected by the genotype, environment, and agronomic management practices. The aim of the research has been to investigate the effect of the environment and agronomic factors on the mineral composition and baking value of winter wheat grain. Opal cultivar grain of the genetically determined prime-quality wheat was obtained in a two-year field experiment (varied soil and weather). The agronomic management practices included tillage (conventional moldboard-plow, reduced ploughless, and strip-till) and nitrogen fertilisation rate (100 kg·N·ha−1, 200 kg·N·ha−1). In the grain samples, the content of macronutrients was assayed: P, K, Mg, Ca, and Na, total protein, and wet gluten as well as sedimentation value. The colour and the water absorption of flour and its content of protein and ash were determined. Laboratory baking was performed. It was found that the content of protein and gluten in grain, sedimentation value, bread volume, and weight changed depending on the environmental conditions and research years. Tillage and nitrogen rate, despite an effect on the properties of grain and flour, did not differentiate, however, the bread quality. The environmental conditions and agronomic management practices did not have a significant effect on the content of mineral nutrients in grain, except for calcium. The biofortification with mineral nutrients in prime-quality winter wheat cultivar grain by selecting the environmental and agronomic conditions seems more difficult than increasing the content of organic compounds and enhancement of flour and bread parameters.

БУЛЯК – ПЕРСПЕКТИВНЫЙ СОРТ ЯРОВОЙ МЯГКОЙ ПШЕНИЦЫ

A new semi-intensive variety of spring soft wheat Bulyak, bred in Tatar Scientific Research Institute of Agriculture, by intraspecific hybridization, the method based on the principle of expected transgressions was based on the selection of parental forms for the creation of the cultivar. The variety has a realized yield potential at the level of 5.5 tons per hectare, the advantage over the three-year test (2014-2016) over the standard Simbirtsit variety in the differing pressure of abiotic and biotic environmental factors is 0.93 tons per hectare, standard in all years of the test. The variety forms a valuable grain in quality. The technological parameters of grain meet the requirements for valuable and strong wheat: nature - 809-815 gramms per litre, vitreousity - 55-87%, protein content in grain – 11.7-13.9%, gluten in grain – 28.2-31.1% of the first and second quality groups. Rheological properties of the dough: strength of flour - 308 units, valorimetric evaluation - 71.3 units, which meets the requirements for strong varieties. The bakery quality is excellent. The variety is medium-ripening, it belongs to the erythospermum (estivum) variety. Middle-growing, height – 82.2 cm, resistance to lodging in the cultivar was not marked below 8 points. The variety has a high-grain ears - 25.6 grains and a high mass of 1000 grains, an average of 42.5 g, in 2014, it reached 47 g. The variety has a low productive bushiness - 1.08 pieces per plant, which is also characteristic of other varieties of selection of the Tatar SRIA. The variety was transferred to the State Variety Test in the Middle Volga and Central regions of the Russian Federation in 2016.

YIELD VARIABILITY OF GRAIN CROP VARIETIES IN THE CONDITIONS OF THE STEPPE ZONE OF WESTERN SIBERIA

The work presents the results of research into the yield and quality of grain obtained from crop varieties of different maturity types, namely spring common wheat varieties (mid-early Pamyati Aziyeva, mid-late Baganskaya 95 and Omskaya 28), spring barley (early-ripening Bagan, mid-ripening Acha and Signal) and spring oats (early-ripening Krasnoobsky, mid-ripening SIG, mid-late Ural 2). The study was conducted in the conditions of the steppe zone of Western Siberia (North Kulunda), the climate of which is extremely continental, and is characterized by signifi cant variability of agrometeorological conditions of the vegetation period by years and a drought during the fi rst half of summer. It was established that sowing of midripening and mid-late varieties of wheat and barley resulted in the increase of the grain yield by 0.47-1.07 t/ha or by 24-30%, compared with the more early-ripening varieties. Among various biotypes of oats, the highest yields of grain were formed by sowing of the mid-ripening variety, which was by 0.60-0.87 t/ha or 19-30% higher than the early and mid-late varieties, whereas the highest yield of green mass was achieved by sowing of the mid-late variety. All varieties of barley, oats and mid-early wheat were characterized by signifi cant variability in grain yields by years (with coeffi cient of variation being 24-38%), while midlate varieties of wheat were characterized by medium or small variability in grain yields (coeffi cient of variation being 3-12%). The content of crude gluten in the grain of wheat varieties varied considerably depending on agrometeorological conditions (coeffi cient of variation being 20-25%). On average, over the years of research, the highest content of gluten (34.0%) was in the grain of the mid-early variety Pamyati Aziyeva, the lowest content (27.4%) was in the mid-late Baganskaya 95. The maximum amount of crude gluten in the wheat grain (33.6-40.0%) was observed in the dry year, the minimum (19.9-26.4%) was in the year with favorable humidity. The content of crude protein in the barley grain varied in the experiment from 11.6 to 14.9% (coeffi cient of variation being 9-14%) and did not differ signifi cantly by varieties (12.5-12.9%). The greatest amount of crude protein in the grain of all varieties of barley (13.7-14.9%) was recorded in the dry year.

Breeding value of varieties of soft spring wheat on grain quality

The results of study 180 collection samples of soft spring wheat of different ecologi-cal and geographical origin according to the grain quality are given. Variability of grain quality depending on the cultivar and growing conditions is given. The greatest variability for years was marked in such indicator as gluten content in grain (Cv=4,2–20,5 %), the smallest – grain nature (Cv =0,3-4,9%). The highest variability of the values of the geno-typic coefficients of variation have vitreousness and quality of gluten in grain (Cv=11,9-14,1%; Cv=14,2-18,3%), the lowest one in grain nature (Cv=3,9-6,4%). The most valuable source material for soft spring wheat breeding is Novosibirskaya 91, Annet, Tarskaya 10, Biryusa, Ekaterina, Duo Chernozemya, Granit.

Detection and Sourcing of Gluten in Grain with Multiple Floating-Gate Transistor Biosensors.

We report a chemically tunable electronic sensor for quantitation of gluten based on a floating-gate transistor (FGT) architecture. The FGTs are fabricated in parallel and each one is functionalized with a different chemical moiety designed to preferentially bind a specific grain source of gluten. The resulting set of FGT sensors can detect both wheat and barley gluten below the gluten-free limit of 20 ppm (w/w) while providing a source-dependent signature for improved accuracy. This label-free transduction method does not require any secondary binding events, resulting in a ca. 45 min reduction in analysis time relative to state-of-the-art ELISA kits with a simple and easily implemented workflow.

КАЧЕСТВО ЗЕРНА И ЗАСУХОУСТОЙЧИВОСТЬ СОРТОВ ОЗИМОЙ МЯГКОЙ ПШЕНИЦЫ

Grain quality and drought tolerance of the winter soft wheat varieties The article considers the study of the traits of winter soft wheat (mass share of protein and gluten) and the level of drought tolerance of these samples under natural conditions. During the years of study the trait “mass share of protein” ranged from 12.8% (the variety “Kipchak”) to 16.3% (the line “1062/09”). The genotypes of the lines “1026/09” (15.0%), “1401/09” (14.9%), “1127/09” (14.8%), the varieties “Nakhodka” (14.8%), “Aksiniya” (14.7%), “Shef” (14.7%) and “Etyud” (14.7%) have been selected due to their maximum average mass share of protein. These samples can be used as the sources of large mass share of protein in the breeding programs. The mass share of gluten in grain of the studied varieties ranged from 23.0% (the variety “Ermak”) to 28.4% (the variety “Nakhodka”). The varieties “Nakhodka” (CV = 1.6%; 28.4%) and “Shef” (CV = 2.0%; 28.0%) have been selected due to the combination of a low coefficient of variation and large mass share of gluten in grain. On average all studied samples had high level of heat tolerance (I st group), the trait varied from 74.3% (the variety “Kazachka”) to 98.4% (the variety “Etyud”). The varieties “Nakhodka” (72.2%) and “Shef” (79.7%) have shown high indexes of drought tolerance and large content of gluten in grain (28.4% and 28% respectively). The study of correlation between mass share of protein and gluten have revealed an average positive correlation (r = 0.67 ± 0.20). The average feedback of protein content and drought tolerance was (r = -0.51 ± 0.23). There is an average direct correlation between heat tolerance and mass share of protein (r = 0.63 ± 0.20) and an average correlation with mass share of gluten in grain (r = 0.57 ± 0.22). The varieties “Nakhodka” and “Shef”, having high level of drought tolerance and mass share of protein and gluten in grain, can be recommended for introducing them into the breeding programs on drought tolerance and grain quality.

Seed quality of winter wheat varieties after black fallow depending on organo-mine­ral fertilizer application in the Left-Bank Forest-Steppe zone of Ukraine

Purpose. To determine the effect of the basic organo-mineral fertilizer on the formation and stability of grain quality of winter wheat varieties in multiple crop rotation after black fallow as a predecessor.Methods. Field experiments were based on a multifactorial scheme using split-plot method with due regard to all requirements of the field experiment procedure, analysis of variance was used for statistical processing of the obtained results.Results. Investigation data was given concerning determination of grain quality indices in winter wheat varieties of diffe­rent ecotypes after black fallow as a predecessor depending on organo-mineral fertilizer application in the Left-Bank Forest-Steppe zone of Ukraine. In average for the period of investigation (2011–2015), the highest protein content in winter wheat grains was formed in no treatment variant [in such varieties as ‘Doridna’ (14.1%), ‘Dykanka’ (14.3%) and ‘Levada’ (14.2%)] and in case of organo-mineral fertilizer application [in the varieties ‘Hordovyta’ (14.0%), ‘Kalyta’ (14.0%), ‘Dykanka’ (14.7%) and ‘Levada’ (14.6%)]. The highest content of crude gluten in grains, without regard for the variant of the experiment, was found in the following varieties as ‘Dykanka’ (24.9–25.1%) and ‘Levada’ (23.7–25.4%).Conclusions. It was established that the content of protein and crude gluten in grains as well as the falling number of winter wheat was highly dependent on such factors as the variety and the year of cultivation as compared to the fertilizer background. The following varieties as ‘Hordovyta’, ‘Mulan’, Dykanka’ and ‘Levada’ were very sensitive to the application of organo-mineral fertilizer for the protein content, while ‘Hordovyta’ (2.4%), ‘Levada’ (1.7%), ‘Borvii’ (1.2%) and ‘Mulan’ (1.1%) – for the crude gluten content.

MICROBIAL PREPARATIONS AND GROWTH REGULATORS AS A MEANS OF BIOLOGIZATION IN AGRICULTURE

The article shows influence of seed treatments with microbial agents and plant growth regulators on growing conditions, yield, content and grain quality of winter wheat, as well as the impact of biopreparations on crop residues’ degradation. It is found that the use of biological products for treating seeds and crops in comparison with control conditions improves the growth and development of winter wheat plants, reduces the incidence of diseases (leaf rust and septoria), increases yield and affect the accumulation of a gluten in grain. Most high profitability provided seed treatment and processing of the autumn crop of winter wheat plant with growth regulator Vitazim, which in addition to the direct impact on the growth and development of plants, stimulated the activity of native microorganisms of chernozem soil.

Effects of grain species and cultivar, thermal processing, and enzymatic hydrolysis on gluten quantitation

Gluten from wheat, rye, and barley can trigger IgE-mediated allergy or Celiac disease in sensitive individuals. Gluten-free labeled foods are available as a safe alternative. Immunoassays such as the enzyme-linked immunosorbent assay (ELISA) are commonly used to quantify gluten in foods. However, various non-assay related factors can affect gluten quantitation. The effect of gluten-containing grain cultivars, thermal processing, and enzymatic hydrolysis on gluten quantitation by various ELISA kits was evaluated. The ELISA kits exhibited variations in gluten quantitation depending on the gluten-containing grain and their cultivars. Acceptable gluten recoveries were obtained in 200mg/kg wheat, rye, and barley-spiked corn flour thermally processed at various conditions. However, depending on the enzyme, gluten grain source, and ELISA kit used, measured gluten content was significantly reduced in corn flour spiked with 200mg/kg hydrolyzed wheat, rye, and barley flour. Thus, the gluten grain source and processing conditions should be considered for accurate gluten analysis.

Влияние рельефа поля на урожай и качество зерна яровой пшеницы

Цель исследований заключалась в изучении влияния склонов различной экспозиции на поражённость растений яровой мягкой пшеницы корневыми гнилями, а также на урожайность посевов и качество зерна. Опыты проводили в период с 2010 по 2011 г. на полях УНЦ ФГБОУ СПО «Аксеновский сельскохозяйственный техникум», а также на полях прилегающих хозяйств Альшеевского района, расположенных на южном крыле Бугульмино-Белебеевской возвышенности. В соответствии с этим ставилась задача выявления экспозиций и участков склона, способных обеспечивать получение максимального урожая зерна высокого качества. Исследования вели в годы с резко контрастными погодными условиями. Урожайность посевов определяли сплошным обмолотом всех растений учетной площадки в фазу полной спелости зерна и последующим взвешиванием урожая. В качестве контроля выступал выровненный участок, на котором по аналогичной схеме закладывали опытные площадки. Яровая мягкая пшеница, размещенная на склоновых землях, в 1,3 – 1,6 раза чаще поражается корневыми гнилями, а ее урожайность на 6,4 – 38,0 % ниже, чем на выровненном участке. В условиях Бугульмино-Белебеевской возвышенности яровую мягкую пшеницу лучше высевать в серединной части склона, так как это позволит получать урожаи зерна на уровне 22,9 – 26,6 ц/га, что на 1,5 – 18,3 % больше, чем в верхней и нижней частях склона. При этом содержание сырой клейковины в зерне достигает 26,9 – 28,6 %, что на 2,9 – 5,6 % выше, чем в зерне, полученном на других частях склона.

Enhancing Efficacy of Azotobactor and Bacillus by Entrapping in Organic Matrix for Rice Cultivation

Inorganic fertilizers release numerous hazardous gases into the atmosphere by volatilization as well as release ionic radicles into surface runoff and ground water by leaching. There is need to develop some sound alternatives to enhance crop productivity without harming the environment. This study was conducted to evaluate the effects of ecofriendly organic matrix-entrapped biofertilizers (OMEB) (Azotobacter chroococcum+Bacillus subtilis) on Basmati rice (Oryza sativa L cv. Basmati). The entrapped biofertilizers significantly increased plant growth, that is, fresh and dry weights, root length, root, leaf and tiller numbers, soluble protein, organic nitrogen, and free ammonium in leaves over free biofertilizers. The crop productivity, that is, grain and straw yield, and quality of grains in terms of grain protein, percentage of moisture, grain starch, wet gluten in grains, as well as NPK in grains and straw were also enhanced by OMEB. We concluded that the efficacy of free biofertilizers can be enhanced by entrapping them in appropriate carriers and providing protection to the biofertilizer microbes. It appears that organic matrix entrapped biofertilizers can be developed as effective organic alternative to conventional chemical fertilizers for rice cultivation in semiarid subtropical agroecosystem.

EVALUATION OF QUALITY INDICATORS RELATED TO QUALITY BREAD WHEAT PROMISING LINES

The bread waste is one of the important socio-economic's issues country now, the urgent need is feeling to improve the wheat quality. Therefore, using the methods of farming and breeding is necessary to improve the quality of this strategic product. As a result, tests of quality's traits in wheat promising lines in Isfahan climate took place. In this study, the choice 17 advanced lines of compare the performances,s experiments, an experiment was conducted for two consecutive cropping (2011-2012) at cultural experiment and research centre in Isfahan located in Kabutar Abad region. Randomized complete block designs with 3 replications were compared with Spring variety (for control). Traits including: 1000 grain weight, hectolitre weight, protein content, Zeleny sedimentation rate, bread volume, grain moisture content, grain hardness, water absorption, falling number, percentage of dry gluten, gluten index, sedimentation rates were SDS.The results of the combined analysis of variance qualitative characteristics,s for two consecutive cropping showed that treatments with compare together and control variety had significant influence in 1% probability.Correlation coefficients of two years showed that the compound test significant positive correlation within grain hardness index and protein content, wet gluten and dry deposition rates of SDS. Also, significant positive correlation with the percentage of protein content of dry gluten. In view of the high correlation with protein content of dry gluten (quantity). However, grain hardness and relatively high correlation with SDS sedimentation as an important measure of protein quality. Therefore, the test results of dry gluten grains can be tough to choose in order to improve the quality of wheat bread may be used.

Role of a grade in technologies of cultivation of a winter wheat

On the basis of the lead analysis of references and own supervision it is shown, that grades play an appreciable role in decrease in development of wreckers and illnesses. The share of influence of grades on productivity in technologies of cultivation reaches 15-20 and more %. Consumer qualities of grain improve. The maintenance gluten in grain reached 39%, fluctuation from 25% at grade Немчиновская 24 up to 39% at a grade Moscow 40. On quality gluten all the tested grades are carried to II group.

Effect of differentiated phosphorus and potassium fertilization on winter wheat yield and quality

This study was conducted in 2007-2010, on a farm near Œrem (south of Poznan, Poland). A field experiment was set up in a randomized block design with four replications for each combination tested. The effects of differentiated rates of phosphorous and potassium applied together with a fixed level of nitrogen and magnesium fertilization were investigated. During the experiment, the winter wheat grain yields were high and significantly different between both between fertilizer treatments and when compared with the control. Correlation analysis on relationships between grain yield and nutrient content in wheat leaves at the beginning of stem elongation stage (BBCH31) showed significant relationships for phosphorous, calcium, magnesium, zinc and manganese. Regression analysis proved that the content of zinc in leaves at the BBCH31 stage was the main factor which determined winter wheat grain yield. Furthermore, mineral fertilization significantly increased the content of protein and gluten when compared with the control objects, whereas no significant differences were observed among the fertilized objects. Statistically significant relationships were found between leaf content of N, P, Mg, Zn and Mn at BBCH31 and the accumulation of protein and gluten in wheat grain. Protein and gluten in grain depended on the content of magnesium in leaves at the beginning of stem elongation stage. Weather conditions as a factor significantly influenced grain size uniformity while mineral fertilization had no influence on this trait.

Content of minerals, total protein and wet gluten in grain of spring wheat depending on cropping systems

Content of minerals, total protein and wet gluten in grain of spring wheat depending on cropping systems

Biotype Diets System®: Blood types and food allergies

Purpose: The goal of this research was to determine patterns in adverse food reactions among human biotypes, if any.Design: Blood types A1, A2, B, AB, O and Rh‐negative were correlated to four kinds of food allergies and hypersensitivities.Materials and Methods: Three studies were conducted from 1985 to 2004. Blood types were correlated to food scores from: (1) mRAST‐IgE food allergy tests, (2) mRAST‐IgG food hypersensitivity tests, and (3) T‐cell food hypersensitivity tests (ELISA/ACT® LRA). An allergy history was recorded. Data were evaluated by statistical analyses, including: ANOVA, MANOVA, Mann–Whitney U, Kruskal–Wallis, and Chi Square. Clinical categories were established by means and ranges. (4) Blood‐type specific lectins were included from the scientific literature.Results: Results were reported by blood types and food groups as raw scores and classes, and included the following: blood type A1 reacted most strongly to nuts and beans, eggs, dairy, and nightshades; while type A2 reacted to the same but more strongly to dairy, eggs and gluten grains. Type B reacted most to eggs, nuts and beans, dairy, gluten grains, nightshades, and sugars. Type O reacted most to dairy, eggs, gluten grains, and nightshades. Type AB reacted most to nuts and beans, seafood, eggs, and dairy; while A2B also reacted to gluten grains. Type Rh‐negative was most reactive to eggs, dairy, nuts and beans, and gluten grains. The highest IgE scores were among types B and Rh‐negative. The highest IgG scores were among types O, A2 and Rh‐negative. The most lectins reacted with type AB.Conclusions: The research showed patterns in food group allergies and hypersensitivities based on ABO, A1/A2 and Rh blood types. Biotype Diets is the first diet‐typing system supported by original scientific research, and has greater accuracy and specificity than other systems. More information is available at www.biotype.net.