Gingival Tissue Of Patients Research Articles

The effect of cyclosporin A (CsA) treatment on gingival tissue of patients with Behçet's disease.

Gingival biopsies were obtained at various intervals for a period of two years from 12 patients suffering from Behçet's disease who were under a clinical trial of cyclosporin A (CsA) treatment. The levels of nondialyzable hydroxyproline (Hypro) were determined in the medium of the cultured tissues. Histologic examinations were also performed every three months. Correlations between the CsA blood levels and the levels of nondialyzable Hypro indicated a reciprocal relationship, especially at blood levels of CsA higher than 600 ng/ml. Histologic examination of gingival sections from CsA-treated patients showed swelling of the epithelial cells, formation of perinuclear clear zones, widening of intercellular gaps and formation of several basal cell layers. In addition, foci of PAS-positive material were found in both the epithelium and stroma. It is assumed that the gingival enlargement observed in the CsA-treated patients was not due to an increase in tissue collagen but rather to an increase in epithelium combined with an accumulation of noncollagenous extracellular matrix material.

歯周疾患における歯肉組織中の kininase 活性について

Kininase activity of gingival tissue in normal subjects and in patients with periodontal disease has been measured quantitatively in order to clarify the relationship between various periodontal condition and the behaviour of the kininase activity.The kininase activity in either the homogenate or the aceton powder of gingival tissue was assayed by biological method of K. Takeya using guinea pig ileum.The following results were obtained;1) Kininase activity in gingival aceton powder individuals was less variable than the activity in the homogenate from fresh gingival tissues. However, both activities showed remarkable individual variation.2) Kininase activities either in the homogenate or the aceton powder of gingival tissues from 70 individuals were very high.3) Kininase activity of the gingival tissue in patients with periodontal disease was lower than that of normal tissue of the disease.From these results, it is speculated that the kininase activity of gingival tissue is closely related to the various periodontal condition, and it is also suggested from pharmacological actions of kinin and kininase that the kininase activity of gingival tissue may play an important role in the etiology and the progression of periodontal disease.

歯周疾患における Lysozyme 活性の消長に関する研究

Lysozyme activity of gingival tissue from patients with or without periodontal disease has been measured quantitatively in order to clarify the relationship between various periodontal conditions and the behaviour of the lysozyme activity.Following this, lysozyme activites of epithelium and tunica propria of gingival tissue were measured, and the differences of the lysozyme activity were investigated.Variations of lysozyme activity in gingival tissues were also investigated before and after oral administration of lysozyme chloride 90mg per pay for 14 days.The total subjects were 104 Japanese (adults) of the ages between 18 and 58 (including 46 males and 58 females).Gingival tissues obtained from periodontal surgery were weighed, then were immediately homogenized at 7500rpm for 20sec. with cold M/15 phosphate buffer solution by using a cold polytron homogenizer. The homogenate was centrifuged at 4500rpm for 10min. in a refrigerated centrifuge at 0°C. The supernatant fluid was used as tissue extract. Lysozyme activities of these specimens were measured by the turbidimetric method within 12 hours of storage at 4°C.The following results were obtained:1) The total lysozyme activities in gingival tissues of the 104 individuals were very high. However, there was a considerable variation of gingival lysozyme activity in each individual.2) The lysozyme activity of gingival tissue was found to be lowest in youth and increased with age.3) Slight difference in the lysozyme activity of gingival tissue by sex was found, but the difference was not significant.4) The lysozyme activity of the gingival tissue in patients with periodontal diseases was clearly higher than that of normal tissue. The activity of the tissue from acute periodontal condition was higher than that from chronic condition. A remarkable increment of the enzyme activity in proportion to the progression and aggravation of the periodontal disease was observed. Also the difference in the lysozyme activity among each periodontal group was significant.5) The average activity of tunica propria in normal subjects was about 40.7per cent higher than that of epithelium. The average activity of tunica propria in patients with periodontal disease was about 50.7per cent higher than that of epithelium.6) The lysozyme activity of epithelium and tunica propria in gingival tissues increased in proportion to the progression and aggravation of the periodontal disease. A positive statistically significant correlation was found between the activity of epithelium and tunica propria.7) Lysozyme chloride (90mg per day for 14 days) was administered orally to patients with periodontal diseases. After the administration of lysozyme chloride, the lysozyme activity of the tunica propria was greater than that of the epithelium.8) After oral administration of lysozyme chloride, the lysozyme activity of tunica propria in patients with severe marginal periodontitis was found to be significantly decreased.As described above, it was speculated that the lysozyme activity of gingival tissue was closely related to the various periodontal conditions.The lysozyme activity of tunica propria of gingival tissue was higher than that of epithelium.After oral administration of lysozyme chloride, the lysozyme activity of tunica propria was increased, and it is suggested that the lysozyme activity of gingival tunica propria may play an important role in the etiology and the mechanism of healing of periodontal disease.

歯周疾患における Lysozyme 活性の消長に関する研究

Lysozyme activity of gingival tissue from patients with or without periodontal disease has been measured quantitatively in order to clarify the relationship between various periodontal conditions and the behaviour of the lysozyme activity.Following this, lysozyme activites of epithelium and tunica propria of gingival tissue were measured, and the differences of the lysozyme activity were investigated.Variations of lysozyme activity in gingival tissues were also investigated before and after oral administration of lysozyme chloride 90mg per pay for 14 days.The total subjects were 104 Japanese (adults) of the ages between 18 and 58 (including 46 males and 58 females).Gingival tissues obtained from periodontal surgery were weighed, then were immediately homogenized at 7500rpm for 20sec. with cold M/15 phosphate buffer solution by using a cold polytron homogenizer. The homogenate was centrifuged at 4500rpm for 10min. in a refrigerated centrifuge at 0°C. The supernatant fluid was used as tissue extract. Lysozyme activities of these specimens were measured by the turbidimetric method within 12 hours of storage at 4°C.The following results were obtained:1) The total lysozyme activities in gingival tissues of the 104 individuals were very high. However, there was a considerable variation of gingival lysozyme activity in each individual.2) The lysozyme activity of gingival tissue was found to be lowest in youth and increased with age.3) Slight difference in the lysozyme activity of gingival tissue by sex was found, but the difference was not significant.4) The lysozyme activity of the gingival tissue in patients with periodontal diseases was clearly higher than that of normal tissue. The activity of the tissue from acute periodontal condition was higher than that from chronic condition. A remarkable increment of the enzyme activity in proportion to the progression and aggravation of the periodontal disease was observed. Also the difference in the lysozyme activity among each periodontal group was significant.5) The average activity of tunica propria in normal subjects was about 40.7per cent higher than that of epithelium. The average activity of tunica propria in patients with periodontal disease was about 50.7per cent higher than that of epithelium.6) The lysozyme activity of epithelium and tunica propria in gingival tissues increased in proportion to the progression and aggravation of the periodontal disease. A positive statistically significant correlation was found between the activity of epithelium and tunica propria.7) Lysozyme chloride (90mg per day for 14 days) was administered orally to patients with periodontal diseases. After the administration of lysozyme chloride, the lysozyme activity of the tunica propria was greater than that of the epithelium.8) After oral administration of lysozyme chloride, the lysozyme activity of tunica propria in patients with severe marginal periodontitis was found to be significantly decreased.As described above, it was speculated that the lysozyme activity of gingival tissue was closely related to the various periodontal conditions.The lysozyme activity of tunica propria of gingival tissue was higher than that of epithelium.After oral administration of lysozyme chloride, the lysozyme activity of tunica propria was increased, and it is suggested that the lysozyme activity of gingival tunica propria may play an important role in the etiology and the mechanism of healing of periodontal disease.

Investigations for pyronin bodies and fluorescent antibodies in 5:5 diphenylhydantoin gingival hyperplasia

This study indicates that pyronin bodies are found in conjunction with plasma cells and are more discernible in the gingival tissue of patients with Dilantin hyperplasia but are not pathognomonic of Dilantin hyperplasia. Pyronin bodies are seen as readily with hematoxylin and eosin as with other staining methods. A specific antigen-antibody reaction is discredited on the basis of direct staining with fluorescein-conjugated gamma globulins. The connective tissue substance from patients with Dilantin gingival hyperplasia exhibited antigenic properties which were probably located in the reticulin or cementing substance. In the connective tissue of patients with periodontitis the degree of fluorescence was less and the morphology of the antigen site was not so definitive in nature as in Dilantin tissue.