歯周疾患における Lysozyme 活性の消長に関する研究

Abstract

Lysozyme activity of gingival tissue from patients with or without periodontal disease has been measured quantitatively in order to clarify the relationship between various periodontal conditions and the behaviour of the lysozyme activity.Following this, lysozyme activites of epithelium and tunica propria of gingival tissue were measured, and the differences of the lysozyme activity were investigated.Variations of lysozyme activity in gingival tissues were also investigated before and after oral administration of lysozyme chloride 90mg per pay for 14 days.The total subjects were 104 Japanese (adults) of the ages between 18 and 58 (including 46 males and 58 females).Gingival tissues obtained from periodontal surgery were weighed, then were immediately homogenized at 7500rpm for 20sec. with cold M/15 phosphate buffer solution by using a cold polytron homogenizer. The homogenate was centrifuged at 4500rpm for 10min. in a refrigerated centrifuge at 0°C. The supernatant fluid was used as tissue extract. Lysozyme activities of these specimens were measured by the turbidimetric method within 12 hours of storage at 4°C.The following results were obtained:1) The total lysozyme activities in gingival tissues of the 104 individuals were very high. However, there was a considerable variation of gingival lysozyme activity in each individual.2) The lysozyme activity of gingival tissue was found to be lowest in youth and increased with age.3) Slight difference in the lysozyme activity of gingival tissue by sex was found, but the difference was not significant.4) The lysozyme activity of the gingival tissue in patients with periodontal diseases was clearly higher than that of normal tissue. The activity of the tissue from acute periodontal condition was higher than that from chronic condition. A remarkable increment of the enzyme activity in proportion to the progression and aggravation of the periodontal disease was observed. Also the difference in the lysozyme activity among each periodontal group was significant.5) The average activity of tunica propria in normal subjects was about 40.7per cent higher than that of epithelium. The average activity of tunica propria in patients with periodontal disease was about 50.7per cent higher than that of epithelium.6) The lysozyme activity of epithelium and tunica propria in gingival tissues increased in proportion to the progression and aggravation of the periodontal disease. A positive statistically significant correlation was found between the activity of epithelium and tunica propria.7) Lysozyme chloride (90mg per day for 14 days) was administered orally to patients with periodontal diseases. After the administration of lysozyme chloride, the lysozyme activity of the tunica propria was greater than that of the epithelium.8) After oral administration of lysozyme chloride, the lysozyme activity of tunica propria in patients with severe marginal periodontitis was found to be significantly decreased.As described above, it was speculated that the lysozyme activity of gingival tissue was closely related to the various periodontal conditions.The lysozyme activity of tunica propria of gingival tissue was higher than that of epithelium.After oral administration of lysozyme chloride, the lysozyme activity of tunica propria was increased, and it is suggested that the lysozyme activity of gingival tunica propria may play an important role in the etiology and the mechanism of healing of periodontal disease.