Germination Of Barley Research Articles

Investigation into the Relationship between Spermidine Degradation and Phenolic Compounds Biosynthesis in Barley Seedlings under Ultraviolet B Stress.

Barley germination under ultraviolet B (UV-B) illumination stress induces effective accumulation of phenolic compounds in the barley. Spermidine can enhance the biosynthesis of phenolic compounds and alleviate the oxidative damage caused by UV-B. To better understand the function of spermidine, inhibitors of enzymes that are involved in the degradation of spermidine and the synthesis of gamma-aminobutyric acid (GABA), the product of spermidine degradation, were applied to barley germinated under UV-B treatment. The results showed a more severe oxidative damage, and a decrease in phenolic acid contents were observed when spermidine degradation was inhibited. However, GABA application did attenuate an increase in electrolyte permeability and MDA content caused by UV-B induced oxidative damage and improved the respiration rate. Meanwhile, GABA application can elevate the accumulation of phenolic compounds by ca. 20%, by elevating the activities of some key enzymes. Furthermore, the application of GABA, together with the inhibitor of spermidine degradation, can alleviate its suppression of the synthesis of phenolic acids, and resistance to UV-B stress. In conclusion, spermidine alleviated oxidative damage and enhanced the accumulation of phenolic compounds using its degradation product.

Alleviation of Water-Deficit Stress on Seed Germination of Barley and Fenugreek in a Sandy Soil Using Superabsorbent Polymer

Water deficit is one of the major limiting factors of seed crop germination and productivity. Consequently, superabsorbent polymers (SAPs) are among several technologies that enhance water use efficiency, leading to worthy seed germination. The objective of this study was to investigate the potential effect of three rates of SAPs (0.0%, 0.5%, and 1.0% w/w) on the seedling emergence of barley and fenugreek sown in a sandy soil using three replicates in a randomized complete block design. Outdoor pot experiments were conducted in Aswan Province, Egypt. The differences in the final germination percentages (FGPs) were not significant for both seeds, while the application of SAPs enhanced the seedling germination index (GI) with significant differences for both crop seeds. The application of SAP at 0.5% gave the highest GI for barley (2.47 day−1) and fenugreek (2.66 day−1) seeds. The sigmoidal function effectively described the cumulative germination percentage rates for both seeds as a function of time under the SAP levels with R2 greater than 0.992. The maximum rates were 69.4 and 64.6 day−1 for barley and fenugreek seeds at SAP 0.5%, respectively. The corresponding rates for 0.0% SAP were 11 and 12 day−1. The water germination efficiencies (WGE) were 27.76 and 30.04 cm−1 for 0.0% and 0.5% SAP for barley, while they were 23.07 and 29.9 cm−1 for fenugreek. Accordingly, SAPs could represent a promising solution for increasing moisture conservation for seed germination in a sand soil. For strategic management, barley is recommended for growth over fenugreek in a semidried soil.

Agrotechnological evaluation of spring barley cultivation in the conditions of the Middle Urals

The studies were carried out in 2016–2020 on dark grey forest soil in the conditions of the Sverdlovsk region in a long-term stationary experiment. The object of observation was the spring barley Sonnet. The agrotechnological methods of spring barley cultivation were assessed in a two-factor experiment: factor A - crop rotations (precursor); factor B - food background. It was established that in the 0–50 cm layer during the sowing period, soil moisture availability on average for the predecessors varied in the range of 64.4–80.3 mm. The introduction of mineral fertilisers and their combinations with organic fertilisers in field crop rotations increased the reserves of productive moisture by 7–10 mm. The highest total nitrate and ammonium nitrogen content was noted during barley germination. The systematic use of fertilisers increased mineral nitrogen in the arable layer by 6.3–14.3 m/kg compared with the control (11.5–12.8 mg). A close positive relationship was found between the moisture content and mineral nitrogen in the 0–20 cm layer in the phase of barley seedlings. The correlation coefficient (r) was 0.67. During the crop rotation, there was no noticeable difference between the predecessors regarding the effect on the barley yield. In control, it varied at 2.63–2.77 t/ha. On fertilised nutrition backgrounds, an excess of grain harvest by 0.2–0.47 t/ha was revealed hectares when placing it as the second crop after the pure fallow to the occupied. On average, for predecessors, fertilisers provided an additional grain harvest at 1.53–1.54 t/ha compared to the control (2.71 t/ha). The organometal background of nutrition was not inferior to mineral fertilisers in terms of its impact on the productivity of grain forage crops—payback 1 kg a.i. Depending on the predecessor on the mineral background of nutrition, Fertilizers varied from 13.2 to 19.1 kg of grain. The maximum was noted in the grain-grass crop rotation. With a combination of organic and mineral fertilisers, the return decreased to 8.4–12.0 kg. A strong correlation was revealed between barley yield and mineral nitrogen content in the arable layer. On average, for the growing season, it was r = 0.97.

Bacteria Stenotrophomonas are Promising Agents for the Treatment of Fat-containing Sewage

The results of monitoring the biological properties of indigenous bacterial strains isolated from soils, which for a long time were in contact with highly concentrated wastewater from the slaughterhouse of meat processing units, were presented. The resulting strains are able to grow on slaughterhouse wastewater in the presence of animal fats as a carbon source in concentration exceeding the maximum acceptable level for wastewater intended for biological treatment with activated sludge, it demonstrated combined high growth rates and resistance to chlorides. The stimulating effect of wastewater treated by combined indigenous bacterial strains on the germination of barley and corn seeds was confirmed. These strains were identified as Stenotrophomonas rhizophila.

Allelopathic effects of Oldeania alpina (K. Schum.) Stapleton leaf aqueous extract on seed germination and initial seedling growth of two selected crops

Empirical evidence indicates that allelopathic chemicals produced by perennials plants result in a positive or negative change in the germination, growth, and behaviour of other plants growing in close association. This study aimed to investigate the allelopathic effects of Oldeania alpina leaf aqueous extract on germination and initial growth of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) test crops under laboratory conditions. Four different solvents (i.e., hexane, ethyl acetate, methanol, and water) were used to prepare O. alpina leaf extracts. Then, the extracts were subjected to qualitative analysis to identify the presence of allelochemical compounds namely; flavonoids, phenols, tannins, terpenoids, steroids, quinones, coumarins, saponins, and, anthraquinones. Aqueous extract concentrations (i.e., 0 %, 2.5 %, 5 %, 7.5 %, and 10 %) were used to irrigate seeds of test crops using a completely randomized design. The analysis revealed that better germination percentage, speed, and time for both wheat and barley were observed at control while reduced as the concentration of the extract increased up to 10 %. Higher mean shoot growth for wheat (105.2 ± 14.6 mm) and for barley (94.3 ± 30.9 mm) were obtained for the control treatment. A similar trend was observed for root lengths of wheat (104.0 ± 12.4 mm) and barley (65.9 ± 18.4 mm). The mean values decreased for each of the studied parameters as the concentration of the extract increased. Except for anthraquinones, all tested allelochemicals in extracts inhibited germination and initial growth of barley and wheat crops. This implies that planting wheat and barley close to O. alpina may require field-level investigation.

Valorization of Baker Yeast Industry Waste in Agriculture by Improving Germination and Growth of Barley and Pea.

Industrial waste still present an environmental danger for the nature and survival of all living beings. Among these toxic products, the focus has been on liquid effluents from the baker's yeast industry that cause real environmental problems mainly due to their pollutant load and the release of unpleasant odors. In order to minimize these hazards and to take advantage of these wastes for the sake of our environment, the present work consists on valorizing effluents from the baker's yeast industry on barley (Hordeum vulgare) and pea (Pisum sativum), two important agricultural products of Tunisian north-west. Results showed that this waste is characterized by its richness in organic matter, and the presence of proteins traces with high chemical and biochemical oxygen demand (COD and BOD5) values. Diluted effluent at a dose of 2.5mg/g significantly improves germination of both plant seeds by germination index (GI) calculation, to reach a maximum of 190 ± 17% and 150 ± 14% for barley and pea, respectively. In fertigation experiment, the use of a lower dose of .62mg/g of diluted effluent promotes plant length to reach 52 ± 4cm and 45 ± 1.4cm, respectively, for H. vulgare and P. sativum. Gas chromatography coupled to mass spectrometry (GC-MS) analysis after derivatization showed significant enhancement of auxin production in pea treated with .62mg/g of cream compared to control with a concentration of 10.60 ± .81 and 8.16 ± .43ng/gFW, respectively. In another experiment, the irrigation of pea plants with furfural, as major compound of cream, promotes length and auxin production to reach 9.89 ± .56ng/gFW for a furfural dose of .31mg/g. This leads us to valorize baker's yeast effluent as an environment-friendly natural product in pea and barley agricultural and give insight to its mode of action.

Spatially resolved transcriptomic analysis of the germinating barley grain.

Seeds are a vital source of calories for humans and a unique stage in the life cycle of flowering plants. During seed germination, the embryo undergoes major developmental transitions to become a seedling. Studying gene expression in individual seed cell types has been challenging due to the lack of spatial information or low throughput of existing methods. To overcome these limitations, a spatial transcriptomics workflow was developed for germinating barley grain. This approach enabled high-throughput analysis of spatial gene expression, revealing specific spatial expression patterns of various functional gene categories at a sub-tissue level. This study revealed over 14000 genes differentially regulated during the first 24 hafter imbibition. Individual genes, such as the aquaporin gene family, starch degradation, cell wall modification, transport processes, ribosomal proteins and transcription factors, were found to have specific spatial expression patterns over time. Using spatial autocorrelation algorithms, we identified auxin transport genes that had increasingly focused expression within subdomains of the embryo over time, suggesting their role in establishing the embryo axis. Overall, our study provides an unprecedented spatially resolved cellular map for barley germination and identifies specific functional genomics targets to better understand cellular restricted processes during germination. The data can be viewed at https://spatial.latrobe.edu.au/.

Identification of potential fungal biocontrol agents for the management of ripgut brome

Ripgut brome (Bromus diandrus) is a significant weed in arable systems with cereals being negatively impacted through yield reduction and contamination of harvested grain. There are limited herbicide options for its control, and reduction of the weed seed bank is a key part of its management. Ripgut brome seeds were incubated in three soils known to harbour a range of plant pathogens, and a range of fungi were subsequently isolated from ungerminated seeds. The most prevalent putative fungal pathogens isolated were Fusarium spp. and Marasmius sp. Selected isolates were cultured on artificial media and tested for their ability to inhibit germination of ripgut brome seeds in a series of in vitro assays, with many isolates completely inhibiting germination. When isolates were tested for their activity against non-target arable species in plant assays, three of five Fusarium spp. isolates significantly inhibited germination of oats and barley while a Marasmius sp. isolate had no effect on germination of these species. Further plant assays using four Marasmius sp. isolates against a wider range of plant species (oats, barley, ryegrass, brome, cocksfoot, and prairie grass) indicated some variability between Marasmius sp. isolates, with two isolates slightly inhibiting germination of some plant species, while two isolates (W14, W17) had no adverse effects. Sequencing of the ITS region of the Marasmius sp. isolates indicated that they were closely related but distinct from M. graminum, which is known to have some phytopathogenic activity and M. oreades which has not yet been reported as a plant pathogen. Further investigation of the Marasmius isolates as potential biocontrol agents for ripgut brome seed is warranted.

Plant growth promoting rhizobacteria and biochar production from Parthenium hysterophorus enhance seed germination and productivity in barley under drought stress.

Drought stress can significantly affect plant growth and development. Biochar (BC) and plant growth-promoting rhizobacteria (PGPR) have been found to increase plant fertility and development under drought conditions. The single effects of BC and PGPR in different plant species have been widely reported under abiotic stress. However, there have been relatively few studies on the positive role of PGPR, BC, and their combination in barley (Hordeum vulgare L.). Therefore, the current study investigated the effects of BC from Parthenium hysterophorus, drought tolerant PGPR (Serratia odorifera), and the combination of BC + PGPR on the growth, physiology, and biochemical traits of barley plants under drought stress for two weeks. A total of 15 pots were used under five treatments. Each pot of 4 kg soil comprised the control (T0, 90% water), drought stress alone (T1, 30% water), 35 mL PGPR/kg soil (T2, 30% water), 2.5%/kg soil BC (T3, 30% water), and a combination of BC and PGPR (T4, 30% water). Combined PGPR and BC strongly mitigated the negative effects of drought by improving the shoot length (37.03%), fresh biomass (52%), dry biomass (62.5%), and seed germination (40%) compared to the control. The PGPR + BC amendment treatment enhanced physiological traits, such as chlorophyll a (27.9%), chlorophyll b (35.3%), and total chlorophyll (31.1%), compared to the control. Similarly, the synergistic role of PGPR and BC significantly (p< 0.05) enhanced the antioxidant enzyme activity including peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) to alleviate the toxicity of ROS. The physicochemical properties (N, K, P, and EL) of the soils were also enhanced by (85%, 33%, 52%, and 58%) respectively, under the BC + PGPR treatment compared to the control and drought stress alone. The findings of this study have suggested that the addition of BC, PGPR, and a combination of both will improve the soil fertility, productivity, and antioxidant defense systems of barley under drought stress. Therefore, BC from the invasive plant P. hysterophorus and PGPR can be applied to water-deficient areas to improve barley crop production.

Effect of Abiotic Stresses from Drought, Temperature, and Density on Germination and Seedling Growth of Barley (Hordeum vulgare L.).

Seed germination and seedling growth are highly sensitive to deficit moisture and temperature stress. This study was designed to investigate barley (Hordeum vulgare L.) seeds' germination and seedling growth under conditions of abiotic stresses. Constant temperature levels of 5, 10, 15, 20, 25, 30, and 35 °C were used for the germination test. Drought and waterlogging stresses using 30 different water levels were examined using two methods: either based at 1 milliliter intervals or, on the other hand, as percentages of thousand kernel weight (TKW). Seedling density in a petri dish and antifungal application techniques were also investigated. Temperature significantly impacted germination time and seedling development with an ideal range of 15-20 °C, with a more comprehensive range to 10 °C. Higher temperatures reversely affected germination percentage, and the lower ones affected the germination and seedling growth rate. Germination commenced at 130% water of the TKW, and the ideal water range for seedling development was greater and more extensive than the range for germination, which means there is a difference between the starting point for germination and the seedling development. Seed size define germination water requirements and provides an objective and more precise basis suggesting an optimal range supply of 720% and 1080% of TKW for barley seedling development. A total of 10 seeds per 9 cm petri dish may be preferable over greater densities. The techniques of priming seeds with an antifungal solution (Bordóilé or Hypo) or antifungal application at even 5 ppm in the media significantly prevented fungal growth. This study is novel regarding the levels and types of abiotic stresses, the crop, the experimental and measurement techniques, and in comparison to the previous studies.

Isolation and characterization of a plant growth‑promoting rhizobacterium strain MD36 that promotes barley seedlings and growth under heavy metals stress.

Plant growth, promoting, bacteria, (PGPB) can improve plant germination and growth in heavy metal-contaminated land and enhance heavy metal removal efficiency. In this study, we isolated PGPR bacterial strains which can withstand heavy metal pollution and tested their ability to improve barley germination under heavy metal stress. Out of 16 multi-resistant heavy metal isolates, strain MD36 was identified by 16S rRNA sequencing and shared close relation to different species of the genus Glutamicibacter. The new isolated strain showed other important PGPR activities, mainly IAA production and salt tolerance. The effect of adding the strain MD36 to barley grains under heavy metal stress enhanced their germination up to 100%, while the percentage of germination ranged between 0 and 20% for non-inoculated grains. In addition, in these conditions, MD36 can significantly enhance barley growth by reducing the heavy metal effect. This study strongly recommends the use of MD36 as seed coatings trials in the field to enhance growth and yield in soils contaminated with heavy metals, as well as in bioremediation of HM-contaminated salt-containing soils and water.

Suitability of Volcanic Ash, Rice Husk Ash, Green Compost and Biochar as Amendments for a Mediterranean Alkaline Soil

Today’s agriculture has the challenge of ensuring food supply for a growing population while human activity has already deteriorated about 40% of the world’s soils, reducing productive capacity and increasing reliance on mineral fertilizers. In this context, valorizing and recycling mineral and agricultural waste for use as substrates or soil supplements enhance a sustainable economy, as well as the development of activities focused on finishing the soil nutrients’ cycle. Looking for an effective solution to the massive waste generation and to enhance the agronomic qualities of soils, this study investigates the agronomic impact of contrasting inorganic and organic materials such as green compost (GC), wood biochar (WB), rice husk ash (RA), and volcanic ash (VA) as amendments to an alkaline Luvisol under controlled conditions. In this sense, barley seeds were planted and grown in a greenhouse under controlled conditions for 60 days on a soil amended with the aforementioned materials. The amendments demonstrated appropriate attributes for improving soil agronomic properties, enhancing the soil’s nutritional content with no effect on barley germination. The WB showed high aromaticity and abundance of refractory organic C. Both ash-rich amendments showed high P and K contents, which are important elements for plant development. The GC has high water retention capacity and an adequate C and N balance. Although the application of the amendments had no effect on barley yields, the plants from the ash-amended pots showed an increase of Photosystem II efficiency, indicative of a better physiological status. In terms of toxicological safety, the abundance of trace elements in soils and plants was investigated. All soils met the maximum allowable limits for these persistent pollutants. Nevertheless, longer-term tests on plants are required to determine the risk of Pb accumulation, particularly in soils amended with GC and compost-ash mixtures. The simultaneous combination of organic and inorganic amendments showed adequate agronomic attributes. WB analysis revealed its great recalcitrance and carbon sequestration potential.

Thermal treatment of petroleum-contaminated marine sediment according to oxygen availability and temperature: Product quality as a potential plant-growth medium

The sustainable management of dredged sediment from contaminated sites needs to consider the end-use of the treated sediment. In this regard, modifying conventional sediment treatment techniques to generate a product that is suitable for a range of terrestrial uses is necessary. In the present study, we evaluated the product quality of treated sediment as a potential plant-growth medium following the thermal treatment of marine sediment contaminated by petroleum. The contaminated sediment was subject to thermal treatment at temperatures of 300, 400, or 500 °C, and no, low, or moderate oxygen availability, and the resulting treated sediment was analyzed in terms of its bulk properties, spectroscopic properties, organic contaminants, water-soluble salts and organic matter, and the leachability and extractability of heavy metals. All operational combinations for the treatment process reduced the total petroleum hydrocarbon content of the sediment from 4922 mg kg−1 to lower than 50 mg kg−1. The thermal treatment process stabilized the heavy metals in the sediment, reducing the zinc and copper concentration by up to 58.9% and 89.6%, respectively, in the leachate from the toxicity characteristic leaching procedure. The hydrophilic organic and/or sulfate salt byproducts of the treatment were phytotoxic, but these can easily be removed by washing the sediment with water. By combining the sediment analysis results with experimental data from barley germination and early-growth tests, the end product was found to be of higher quality when higher temperatures and lower oxygen availability were employed in the treatment process. These findings demonstrate that it is possible to retain the natural organic resources of the original sediment by optimizing the thermal treatment, thus ensuring a suitably high product quality for use as a plant-growth medium.

Effect of highland barely germination on thermomechanical, rheological, and micro-structural properties of wheat-oat composite flour dough

This study investigated the effects of highland barley's germination time (24, 48, and 72 h) on the thermomechanical, pasting, fermentation, dynamic rheological, molecular mobility, and protein structural properties of oat-wheat composite flour dough. Germination significantly reduced flour water absorption, freezable water, melting enthalpy, and increased protein weakening compared with native barley (NB) samples. In addition, germination decreased bound and immobilized water and increased the free water compared to the NB-composite flour dough samples, according to the results of a low-field nuclear magnetic resonance spectrometer (LF-NMR). Germination also increased the protein's extractability and surface hydrophobicity while negatively impacting the starch-protein network; however, it decreased bread-specific volume. Germination for 24 h, on the other hand, had minor effects on dough and bread samples compared to 48 and 72 h. As a result, germination for 24 h under controlled conditions could be used to improve the rheological qualities of highland barley and avoid the adverse rheological effects of the prolonged germination period.

The competitive strategies of poisonous weeds Elsholtzia densa Benth. on the Qinghai Tibet Plateau: Allelopathy and improving soil environment.

The competitive strategies of plants play a crucial role in their growth. Allelopathy is one of the weapons that plants use to improve their competitive advantage. In order to explore the competitive strategy of a poisonous weed Elsholtzia densa Benth. (E. densa) on the Qinghai-Tibet Plateau (QTP), the effects of decomposing substances of E. densa on growth, root border cells (RBCs) characteristics of highland crop highland barley (Hordeum vulgare L.), and soil environment were determined. The decomposing allelopathic effect of E. densa on the germination and seedling growth of highland barley mainly occurred in the early stage of decomposing. The allelopathic effects were mainly on seed germination and root growth of highland barley. After treatment with its decomposing solution, the RBC's mucilage layer of highland barley thickened, and the RBC's activity decreased or even apoptosis compared with the control. However, only the above-ground part of the treatment group showed a significant difference. The effects of E. densa decomposed substances on the soil environment were evaluated from soil physicochemical properties and bacterial community. The results showed that soil bacteria varied greatly in the early stage of decomposion under different concentrations of E. densa. In addition, E. densa decomposing substances increased the soil nutrient content, extracellular enzyme activities, and bacterial community diversity. In the process of decomposition, the bacterial community structure changed constantly, but Actinobacteriota was always the dominant phylum. These results indicated that E. densa might adopt the following two strategies to help it gain an advantage in the competition: 1. Release allelochemicals that interfere with the defense function of surrounding plants and directly inhibit the growth and development of surrounding plants. 2. By changing the physical and chemical properties of soil and extracellular enzyme activity, residual plant decomposition can stimulate soil microbial activity, improve soil nutrition status, and create a more suitable soil environment for growth.

Gibberellic acid effect and different salt concentrations on barley germination quality | IJAAR 2022

Gibberellic acid effect and different salt concentrations on barley germination quality | IJAAR 2022

Allelopathic Effects of Cannabis sativa L. Aqueous Leaf Extracts on Seed Germination and Seedling Growth in Durum Wheat and Barley

In this study, the allelochemical-induced effects of the leaf water extract of hemp (Cannabis sativa) on seed germination and seedling growth were investigated in durum wheat (Triticum durum) cv. Mongibello and barley (Hordeum vulgare) cv. Alamo. The seeds were imbibed in 25%, 50%, 75%, or 100% solution concentrations, obtained from a 2% hemp leaf extract, and they were germinated at 20 and 10 °C. The 2% extract stock solution (i.e., 100% solution concentration) was obtained by extracting 20 g of leaf powder in 1 L distilled water (2%, w/v). A control of seeds imbibed in distilled water was considered. Germination was scored daily and at the end of the germination test, the final germination percentage (FGP), mean germination time (MGT), germination index (GI), and vigor index (VI) were calculated. The radicles and shoots of the germinated seeds of both species were also measured for their length and weight. The analyses revealed the presence of phenols in the hemp leaf extract, which were the highest in the 100% concentration (13.14 ± 0.20 mg gallic acid equivalent-GAE g−1 extract). At 20 °C, increasing the concentration had either minor (in wheat) or no effects (in barley) on the seed germination. At 10 °C, the germination was reduced by the increasing concentration, suggesting a potential allelopathic effect of the extract. However, at 100% concentration, the seeds germinated significantly better that those at 75% concentration. At 20 °C, increasing concentration progressively depressed both the radicle and shoot elongation in both species. At 10 °C, the radicle elongation was depressed by the extract, irrespective of the concentration, whilst the shoots were as long as those of the control in wheat, and smaller at the 50 and 75% concentrations in barley. We can conclude that the water extract of hemp leaves can have a negative impact on wheat and barley seed germination and seedling growth, particularly at temperatures below optimal. However, the high concentration of phenols in the extract may stimulate seed germination and seedling growth. Thus, in semi-arid areas, such as those of the Mediterranean regions, sowings of wheat and barley after hemp at an optimal (i.e., 20 °C) or nearly optimal temperature are recommended in order to minimize the combined stressing effects of the temperature and allelopathic leaf extract upon plant establishment, which may limit a successful crop establishment.

Salisilik Asit Uygulamalarının Tuz Stresi Altındaki Arpa (Hordeum vulgare L.) Çeşitlerinde Çimlenme ve Tohum Özellikleri Üzerine Etkisinin Belirlenmesi

Ordu Üniversitesi Ziraat Fakültesi Tarla Bitkileri Laboratuvarında 2022 yılında yürütülen bu çalışmada tuz stresi altındaki arpa çeşitlerinde salisilik asit uygulamalarının çimlenme ve tohum özellikleri üzerine etkilerinin belirlenmesi amaçlanmıştır. Bitki materyali olarak 3 farklı arpa çeşidi (Larende, Beyşehir, Kral) kullanılmıştır. Dört farklı tuz konsantrasyonu (0-50-100-150 mM) ve dört farklı salisilik asit konsantrasyonu (0-0.25-0.5-1 mM) arpa tohumlarına uygulanmıştır. Araştırma, tesadüf parsellerinde faktöriyel deneme desenine göre üç tekrarlı olarak yürütülmüştür. Araştırmada çimlenme oranı (%), çimlenme süresi (gün), toplam yaş ve kuru ağırlıK (g), radikula yaş ve kuru ağırlığı (g), koleoptil yaş ve kuru ağırlığı (g), radikula uzunluk (mm) ve koleoptil uzunluk (mm) değerleri hesaplanmıştır. Çalışma sonucunda tüm uygulamalarda en iyi sonuç veren çeşitler Larende ve Beyşehir arpa çeşitleri olmuştur. Tuz dozlarının artmasıyla birlikte hemen hemen incelenen tüm parametrelerde olumsuz yönde değişiklikler meydana gelmiş, salisilik asit uygulamalarıyla ile bu olumsuz değişiklikler tamamen ortadan kaldırılamasa da etkileri azaltılmıştır. Tuzun özellikle 150 mM dozu en kritik değişikliklerin meydana geldiği tuz dozu olarak belirlenmiştir.

The Correlations of Barley Vitality and Storage Reserve Mobilization during Early Germination

The elucidation of the genetic relationship about storage reserve mobilization and enzymatic activity during barley germination is of particular importance for breeding and malt production. In addition to plant hormones such as gibberellic acid (GA) and abscisic acid (ABA), recent research has highlighted the importance of reactive oxygen species (ROS) signaling with hydrolase induction and plant growth. In this study, the correlation of barley vitality and mobilization of storage reserves during early seed germination was investigated. The activity of important hydrolases, as well as ROS-producing NADPH (nicotinamide adenine dinucleotide phosphate) oxidases, was monitored between six different cultivars. Associated gene expression from imbibition to germination was also analyzed by correlation analysis and cluster analysis. During early germination, the activities of α-amylase, limit dextrinase, and β-glucanase had significant positive correlations with each other and affected seed germination. Gene expression analysis showed that RbohB1 and RbohF1 (NADPH oxidases) had strong correlations with AMY1 (α-amylase) and LD (limit dextrinase). The GA synthesis gene GA3ox2 was found to have a significant correlation with protease genes Cys (cysteine protease) and Ser (serine protease), while the ABA synthesis gene NCED1 (nine-cis-epoxycarotenoid dioxygenase) had significant negative correlations with Cys and GA3ox2. Gene expression and enzyme assays indicated that NADPH oxidase, α-amylase, and limit dextrinase correlated well with barley vitality during early germination. The expression of the GA synthesis genes correlated well with glucanase and protease. These results provide information about barley gene regulation by analyzing correlations between genes.

Effect of Some Seaweed Extracts on Germination and Growth of Barley (Hordeum Vulgare L)

Effect of Some Seaweed Extracts on Germination and Growth of Barley (Hordeum Vulgare L)