Evaluation of transcription factor and aquaporin gene expressions in response to Al2O3 and ZnO nanoparticles during barley germination

Abstract

Aluminum oxide and zinc oxide nanoparticles (NPs) are two of the mostly produced engineered metal oxide NPs. Here, barley germination and root elongation as well as gene expressions of the selected aquaporins (HvTip1;1 and HvPip1;1) and transcription factors (HvERFs and HvNFX1) were investigated after exposure to Al2O3 and ZnO NPs for foreseeing the effect of NP exposure. ICP-MS analysis showed that the nanoparticles were taken up into root and leaves. Even the germination analysis and seedling establishment data indicate that the applied NPs do not have any observable inhibitory effects except on root length, the gene expression analysis revealed that these nanoparticle applications lead to a response at the molecular level. The gene expression profiling indicated that aquaporins and transcription factor genes were differentially regulated in leaves and roots in response to NPs treatments. The expressions of aquaporin genes were higher especially in leaves in compared to the control plants. Gradual decrease was obtained in roots by application of the increased levels of Al2O3 NPs. The effects of ZnO NPs on gene expression levels of barley TFs were dramatically more distinctive in comparison with that of Al2O3 NPs. The expression profiles of HvERFs and HvNFX1 transcription factors in response to the Al2O3 and ZnO NPs suggest that these selected TFs can play important roles in shaping abiotic stress tolerance in young barley roots and leaves. Outcomes of the study will allow us to predict complex stress response of barley in response to the nanoparticles.