Enhancement Effect of Chitosan Coating on Inhibition of Deoxynivalenol Accumulation by Litsea cubeba Essential Oil Emulsion during Malting.

Zhengcong Peng,Wenxu Feng,Guolin Cai,Jian Lu,Dianhui Wu

doi:10.3390/foods10123051

Abstract

The purpose of this work was to study the enhancement effect of chitosan coating on inhibition of deoxynivalenol (DON) accumulation by Litsea cubeba essential oil emulsion during malting. Firstly, the primary emulsion suitable for malting process was screened and the improvement effect of chitosan coating on the properties of primary emulsion was studied. On this basis, chitosan-based Litsea cubeba essential oil emulsion was applied to malting processing. The results showed that the primary emulsion of Litsea cubeba essential oil had good antifungal properties and a minimal effect on the germinability of barley compared with other primary emulsions. The addition of chitosan can improve the physical stability and antifungal ability of the emulsion and reduce the effect of the emulsion on barley germination. When 100 g of chitosan-based Litsea cubeba essential oil emulsion (40 mg/g) was applied to the malting process, the germination rate of barley was 87.7% and the DON concentration of finished malt was reduced to 690 μg/kg, which was 20.9% lower than that of the control. Meanwhile, the other indexes of malt produced by secondary emulsion treatment (after adding chitosan) increased significantly compared with those of malt produced by primary emulsion. This study was of great significance for the application of emulsion to inhibit the accumulation of mycotoxin during malting.

Highlights

Deoxynivalenol (DON) is a type B trichothecene mycotoxin, which is produced by Fusarium when it infects cereals, fruits, and vegetables [1,2]
Potatoes and mung beans were purchased from local supermarkets (Wuxi, China), while barley infected with Fusarium head blight was provided by Jiangsu Agricultural Reclamation Malt Company (Sheyang, China)
Thirteen strains of filamentous fungi from barley infected with Fusarium head blight were obtained by plate coating and further purification

Summary

Introduction

Deoxynivalenol (DON) is a type B trichothecene mycotoxin, which is produced by Fusarium when it infects cereals, fruits, and vegetables [1,2]. Barley malt is the main raw material of beer production, which is prepared by soaking, germinating, and baking under a certain temperature and humidity. There is a risk of DON contamination in the production and storage process of malt. DON has good thermal stability and water solubility, which is not easy to remove in the beer production process and will eventually remain in the finished beer [3,4,5]. Studies have shown that excessive intake of DON can produce toxic reactions, and long-term low doses can cause damage to the body [6,7]. The residual of DON in beer will pose a threat to the health of consumers

Objectives

Methods

Results