The ability to overcome an opposition in team sports is reliant upon an understanding of the tactical behaviour of the opposing team members. Recent research is limited to a performance analysts’ own playing team members, as the required opposing team athletes’ geolocation (GPS) data are unavailable. However, in professional Australian rules Football (AF), animations of athlete GPS data from all teams are commercially available. The purpose of this technical study was to obtain the on-field location of AF athletes from animations of the 2019 Australian Football League season to enable the examination of the tactical behaviour of any team. The pre-trained object detection model YOLOv4 was fine-tuned to detect players, and a custom convolutional neural network was trained to track numbers in the animations. The object detection and the athlete tracking achieved an accuracy of 0.94 and 0.98, respectively. Subsequent scaling and translation coefficients were determined through solving an optimisation problem to transform the pixel coordinate positions of a tracked player number to field-relative Cartesian coordinates. The derived equations achieved an average Euclidean distance from the athletes’ raw GPS data of 2.63 m. The proposed athlete detection and tracking approach is a novel methodology to obtain the on-field positions of AF athletes in the absence of direct measures, which may be used for the analysis of opposition collective team behaviour and in the development of interactive play sketching AF tools.