Gas Chromatography-mass Spectrum Research Articles

Volatiles potentially useful to be used as biomarkers for monitoring the khapra beetle Trogoderma granarium for quarantine

The khapra beetle, Trogoderma granarium, is one of the most important quarantine pests globally. In order to identify potential biomarkers for establishing a rapid and effective monitoring technology for T. granarium quarantine, headspace-solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrum (GC-MS) was used to identify volatiles that are potentially useful for early detection of the presence of the T. granarium presence. Three volatiles, including Ethyl palmitate, (Z)-5-tetradecenoic acid and (Z)-14-methyl-8-hexadecen-1-al, were commonly identified as unique volatiles emitted from four different stored products including peanut, barley, wheat and rapeseed only after T. granarium infestation. In addition, four volatiles including hexadecane, palmitic acid, (Z)-14-methyl-8-hexadecen-1-al and N-heptadecane, were found emitted commonly from the larvae of T. granarium after they fed on the four stored products. These common chemicals could potentially be biomarkers sensitive enough for monitoring the presence of T. granarium. In addition to these unique chemicals, a combination of up- and down-regulated volatiles by each type of stored seeds were also identified as potential markers useful for the early detection of the presence of T. granarium. Our results could be a first step towards establishing a method for T. granarium monitoring and quarantine.

Analysing the Bioactive Compound of Earthworm Pheretima Javanica Extract by Using Gas Chromatography Mass Spectrum

Research Based Learning (RBL) is a learning method that uses contextual learning, authentic learning, problem solving, cooperative learning, hands on and minds on learning and inquiry learning. The STEM approach in learning is expected to provide meaning to students through the systematic integration of research-based knowledge, concepts and skills. STEM design begin with the identification of social problems. The social issue of typhoid fever cases caused by Salmonella enterica Typhi bacteria with a high mortality rate in the world is a public health problem. The existence of Pheretima javanica which is abundant in the environment can be utilized by conducting antibacterial activity testing and analysis of bioactive compounds by students on the form of the extract. This research is a development research carried out with the aim of developing a product and also a qualitative descriptive research which serves to provide an overview of the object. The steps in carrying out these tests were identified as part of learning that hone creative thinking skills. The results of the class experimental research showed that the N-Gain score was 0.8, which is in the high category. These results show that the application of developing problem-based learning tools with a STEM approach is effective. Learning outcomes contribute to the effort to find alternative drugs that are effective and do not cause resistance. In addition, students also produce scientific articles as learning products. This paper has implications for STEM integrated research-based learning.

Synthesis, characterization and bioactive evaluation of cobalt(II) and copper(II) complexes of {4-[5-(5-sulfanylidene-2,5-dihydro-1H-1,2,4-triazol-3-yl) furan-2-yl] phenyl} acetic acid

A new triazole derivative was synthesized by three steps. The first step involve adding 2-carboxyaledhyed furan to the 4-amino phenyl acetic acid after converting the substituted aromatic amine to the diazanium salt. In the first stage, [4-(5-formylfuran-2-yl) phenyl] acetic acid is the title of product. The second step is adding the thiosemicarbazide to the [4-(5-formylfuran-2-yl) phenyl] acetic acid which it gives the (4-{5-[(2-carbamothioylhydrazinylidene) methyl] furan-2-yl} phenyl) acetic acid. A final compound with the title {4-[5-(15-sulfanylidene-2,5-dihydro-1H-1,32,4-triazol-3-yl) furan-22-yl] phenyl} acetic acid was produced as a result of adding sodium acetate and chloro ethyl acetate to the product from the second step in the presence of triethyl amine which is used as a ligand to prepare cobalt(II) and copper(II) complexes. The structures of synthesized compounds were identified through the utilization of diverse measures, these measurements were (UV-Vis spectrum, LC-MS technique, FT-IR spectrophotometer, conductivity measurements, GC-mass spectra and atomic absorption) and melting point measurement. The results confirmed that the complexes have octahedral geometry. The biological activity data for the synthesized compounds revealed that the ligand and its complexes had antibacterial activity. KEY WORDS: Synthesis, Triazole derivatives, Cobalt(II) complex, Copper(II) complex, Biological evaluation Bull. Chem. Soc. Ethiop. 2024, 38(6), 1595-1607. DOI: https://dx.doi.org/10.4314/bcse.v38i6.8

GC–MS analysis of Lactobacillus plantarum YW11 metabolites and its computational analysis on familial pulmonary fibrosis hub genes

Abstract The purpose of this research was to examine the interaction between metabolites of Lactobacillus plantarum YW11, characterized through GC–mass spectra (MS) analysis, and the FN1 protein in cases of familial pulmonary fibrosis, found from hub genes analysis. GC–MS analysis was performed to identify metabolites in L. plantarum. Then, gene expression analysis and functional annotations were conducted to investigate the hub genes. A network of hub genes and transcription factors (TFs) was constructed, highlighting the significance of FN1 in the disease’s etiology. Molecular docking was employed to explore the interaction between the characterized metabolites and the FN1 protein. Toxicity analysis was also carried out. Thirty-two active compounds of L. plantarum YW11 were characterized by GC–MS. The gene expression analysis identified 295 differentially expressed genes, including 10 hub genes and 6 TFs, providing further support for the involvement of FN1 protein in the disease. The results of the molecular docking studies suggest the therapeutic potential of targeting FN1, with the best docking result observed for the interaction between FN1 and the 2,4-di-tert-butylphenol metabolite (energy of −6.9 kcal/mol). The toxicity analysis and molecular dynamic simulations support the suitability of 2,4-di-tert-butylphenol as a candidate for targeting FN1.

Identification of the Sex Pheromone of the Asparagus Moth, Parahypopta Caestrum (Lepidoptera, Cossidae)

Chemical, electrophysiological, and field trapping experiments were carried out to identify the female-produced sex pheromone of the asparagus moth, Parahypopta caestrum, a very serious pests of asparagus cultivations in southern Europe. Gas chromatography coupled with mass spectrometry and electroantennogram detection (GC-MS-EAD) analysis of hexane and solid-phase microextraction (SPME) extracts of sex pheromone glands of calling females consistently detected four compounds eliciting EAG responses in male moth antennae. According to their GC retention times, mass spectra, and comparative EAG analyses with reference standards, these EAD-active compounds were identified as (Z)-9-tetradecenol (Z9-14:OH), (Z)-5-tetradecenyl acetate (Z5-14:Ac), (Z)-7-tetradecenyl acetate (Z7-14:Ac), and (Z)-9-tetradecenyl acetate (Z9-14:Ac), respectively. In the SPME extracts from the head-space of individual abdominal tips, Z9-14:Ac, Z5-14:Ac, Z7-14:Ac, and Z9:14 OH were detected in the ratio of 82:9:5:4. In EAG dose-response experiments, Z9-14:Ac was the strongest antennal stimulant at different doses tested. In field trapping experiments, Z9-14:Ac, Z7-14:Ac, and Z5-14:Ac proven to be essential for male attraction and a their 85:5:10 blend loaded onto green rubber septum dispensers was significantly more effective than single-, two-, and any other three-component blend of these compounds. The addition of Z9-14:OH to the optimal blend resulted in a significant reduction of male catches. The attractive blend here identified allowed for an effective and accurate monitoring of P. caestrum flight activity in southern Italy.

Optimization, Production, and characterization of cottonseed methyl ester based on Box-Behnken in response surface design and gas Chromatography-Mass spectrum analysis

Renewable energy sources have a significant approach to eco-friendly systems with economically cost-effective and efficient use of biodiesel. The creation of innovative catalysts is attracting the attention and concern of the chemical and manufacturing sectors to be used in the production of biodiesel. The main objective of this work is to produce the optimum biodiesel yield using BBD design and the base-catalyzed method. The production of biodiesel was achieved through experimental investigation (alkali-catalyzed transesterification) and optimized using the BBD design method.The most important parameters such as methanol to oil ratios, KOH as catalysts, and reaction times were provided with variable conditions. The variation of methanol to oil ratios has significant relationships and is highly correlated in findings. The physicochemical properties of cottonseed biodiesel were carried out according to ASTM and EN standards. The maximum yield was achieved at 96.708 at a molar ratio of 6:1, reaction time of 60 min, and catalyst concentration of 1 wt%. The strong correlation between actual and predicted values of COSME was analyzed using ANOVA variances. The F-value and P-value validated the outcomes of produced biodiesel having a model value of 43.68 and 0.00, respectively. The coefficient of determinations and adjusted R2 of CSOME were achieved at 98.7% and 98.6%, respectively. The gas chromatogram mass spectrum analysis was also obtained to identify the acid profile present in COSME. The major percentage of ester available in COSME has been determined using GC–MS analysis and presented by its weight percentages. The main esters have been including myristic acid methyl ester (1.7%), palmitic acid methyl ester (26.65%), methyl 9-cis,11-trans-octadecadienoate(44.05%), ethyl 9. Cis.11.trans octadecadienoate(9.67%), methyl 20-methyl-heneicosanoate(6.83%), 9,12-Octadecadienoic acid (Z,Z)-, 2-hydroxy-1-(hydroxymethyl)ethyl ester(1.93%), and Pentacosanoic acid, methyl ester(7.14%).

Effect of jujube powder addition on the aroma profile of quinoa snacks (QS).

Quinoa is a full-nutrition food; however, its poor flavor and small size make it not the best food option for direct consumption. In this study, a quinoa snack (QS, a cake) was developed, and the aroma profile of the products was improved by adding jujube fruit powder (made from dried jujube fruits, from 5% to 30%). Gas chromatography mass spectrum (GC-MS) combined with electronic nose (e-nose) was applied for characterizing the aroma profiles of QS samples. Results showed a total of 26 aroma compounds were identified in QS samples by GC-MS, and 3-methylbutanol (from 1525 μg/kg in QS-30 to 3487 μg/kg in QS-0), ethanol (from 1126 μg/kg in QS-0 to 3581 μg/kg in QS-30), hexanal (from 125.6 μg/kg in QS-30 to 984.1 μg/kg in QS-0), and acetaldehyde (from 531.9 μg/kg in QS-30 to 191.1 μg/kg in QS-0) were common. The e-nose response of W1S (sensitive to methane, from 17.50 of QS-0 to 93.85 of QS-30) and W1W (sensitive to sulfur-organic compounds of e-nose, from 15.57 of QS-0 to 39.50 of QS-30) were significantly higher, and significant differences were presented among QS samples. In conclusion, the aroma profile of the QS sample was significantly (p < .05) enhanced by the addition of jujube powder, and QS-30 with the highest jujube content (30%) presented the strongest aroma profile. Moreover, QS samples with different additions of jujube powders could be well distinguished by principal component analysis (PCA), and the combination of e-nose and GC-MS was effective in the volatile profile analysis of QS samples.

Assessment of the chemical profile and potential biocontrol of Amphora coffeaeformis against foodborne pathogens and Culex pipiens L. to ensure food safety

AbstractThe extensive use of synthetic antibiotics and insecticides in controlling microbes and insect spreads led to a build‐up of resistance strains and caused negative impacts on human health through bioaccumulation in food and the environment. The present study assessed the antimicrobial activity of Amphora coffeaeformis (A. coffeaeformis) ethanolic extract against foodborne pathogenic microbes as well as assessed its ability to control Culex pipiens L. (C. pipiens) larvae. The gas chromatography–mass spectrum (GC/MS) and high‐performance liquid chromatography (HPLC) analysis showed that A. coffeaeformis extract was rich with phenolic compounds, organosulfur compounds, carboxylic acids, amino acids, organic quinoline, dipeptide and monosaccharide. The extract of A. coffeaeformis showed antibacterial capability against all tested bacteria, with minimal inhibition concentration (MIC) values ranging from 20.2 to 66.7 g/mL, as well as antifungal effects against all tested mycotoxigenic fungi, with MIC values ranging from 113.62, 68.95 and 49.37 μg/mL mg/mL. Furthermore, the extract showed high larvicidal activity against C. pipiens larvae at 24, 48, and 72 h, respectively. LC50 values decreased gradually with the treatment period. Likewise, the adult emergence and growth index were significantly negatively related to increasing extract concentrations. Moreover, the extract exhibited a noteworthy depletion in the reproductive potential and increased sterility index of C. pipiens females developed of treated larvae. Besides a reduction in egg hatching percent, increase pupal malformations, increase and adult abnormalities. C. pipiens biochemical markers; glutathione‐S‐transferase, carboxylesterase, acetylcholinesterase, α‐esterase and cytochrome P‐450 showed significant alteration after the extract exposure. Overall, these findings promise the application possibility of A. coffeaeformis extract as a biopreservator against foodborne pathogens and as bioinsecticides for mosquito control as well.

Extraction of &lt;i&gt;trans&lt;/i&gt;-Anethole from Star Anise (&lt;i&gt;Illicium verum&lt;/i&gt;) Using Combination of Microwave, Ultrasonic, and Enzyme Assisted Methods and Evaluation of Their Antibacterial Activity

This study examines the efficiency of non-conventional extraction methods to obtain trans-anethole from Illicium verum using single and combination extraction techniques: microwave assisted extraction (MAE), ultrasound-assisted extraction (UAE), and enzyme assisted extraction (EAE) employing lipase from Aspergillus oryzae. All extraction methods were conducted using 96% ethanol (1:5 w/v) with varying time durations. The resulting product was an oleoresin, subsequently analyzed and separated using thin liquid chromatography (TLC) and column chromatography employing a solvent mixture of toluene and ethyl acetate in a 9:1 ratio and identified via gas chromatography-mass spectra (GC-MS). The results revealed that trans-anethole yields from a single extraction method were 30.76% (MAE), 41.05% (UAE), and 40.90% (EAE). The combination of extraction methods, such as MAE-UAE, MAE-EAE, and UAE-EAE, produced trans-anethole yields of approximately 42.73%, 52.80%, and 45.02% respectively, surpassing the yields of the single extraction method. Notably, the triple extraction method of MAE-UAE-EAE yielded the highest trans-anethole content at 56.00%. Antibacterial testing against Staphylococcus aureus was performed on all samples. The trans-anethole demonstrating the highest inhibitory effect was obtained from the double extraction method, particularly the combination of UAE-EAE. These results underscore the synergistic efficiency of combining microwave, ultrasound, and enzymatic extraction methods, highlighting their superior efficacy in obtaining trans-anethole.

Caralluma pauciflora based Ag-NPs activate ROS - induced apoptosis through down-regulation of AKT, mTOR and pI3K signaling in human gastric cancer (AGS) cells

The phytochemicals found in Caralluma pauciflora were studied for their ability to reduce silver nitrate in order to synthesise silver nanoparticles (AgNPs) and characterise their size and crystal structure. Thunbergol, 1,1,6-trimethyl-3-methylene-2-(3,6,9,13-tetram, Methyl nonadecanoate, Methyl cis-13,16-Docosadienate, and (1R,4aR,5S)-5-[(E)-5-Hydroxy-3-methylpent were the major compounds identified in the methanol extract by gas chromatography–mass spectrum analysis. UV/Vis spectra, Fourier-transform infrared spectroscopy, x-ray diffraction, scanning electron microscope with Energy Dispersive X‐ray Analysis (EDAX), Dynamic Light Scattering (DLS) particle size analyser and atomic force microscope (AfM) were used to characterise the Caralluma pauciflora plant extract-based AgNPs. The crystal structure and estimated size of the AgNPs ranged from 20.2 to 43 nm, according to the characterization data. The anti-cancer activity of silver nanoparticles (AgNPs) synthesised from Caralluma pauciflora extract. The AgNPs inhibited more than 60% of the AGS cell lines and had an IC50 value of 10.9640.318 g, according to the findings. The cells were further examined using fluorescence microscopy, which revealed that the AgNPs triggered apoptosis in the cells. Furthermore, the researchers looked at the levels of reactive oxygen species (ROS) in cells treated with AgNPs and discovered that the existence of ROS was indicated by green fluorescence. Finally, apoptotic gene mRNA expression analysis revealed that three target proteins (AKT, mTOR, and pI3K) were downregulated following AgNP therapy. Overall, the findings imply that AgNPs synthesised from Caralluma pauciflora extract could be used to treat human gastric cancer.

Biodiversity and suppression of wood-decaying fungi in marine environment, Alexandria, Egypt by selected natural products: Control the fungal growth/contamination

The current study investigated the biodiversity and control of wood-decaying fungi isolated from marine environment, at Alexandria, Egypt. The fungal growth was in vitro control via selected plant natural products (seaweeds and aromatic herbs). Among 45 marine fungal isolates from decaying-wood samples were detected. At 85% similarity level, 5 clusters (A, B, C, D, and E) were obtained. According to numerical classification, one strain from each cluster was identified based on the sequences of ITS-PCR. The species level because their best matched references from BLAST search were higher than 96% of similarities with ITS sequences obtained from GenBank (four Aspergillus and one Penicillium species as the most abundant isolates). Additionally, the enzymatic action of cellulase, amylase, protease, and lipase was detected by fungal isolates. The highest enzymatic degradation coefficient (EDC) was of 1.38 for lipase secreted by P. chrysogenum strain HGM3. Only three strains (A. sydowi strain HGM1, P. chrysogenum strain HGM3 and A. amstelodami strain HGM5) produced cellulase enzyme with EDC value (1.075, 1.27 and 1.08), respectively. Moreover, the methanolic extracts of Ulva lactuca and Corallina officinalis collected from the Egyptian coasts besides aromatic plant; Salvia. officinalis purchased from herbal market were qualitatively analyzed using Gas chromatographic mass spectra. Various organic crude extracts exhibited that there were different types of high and low molecular weight bioactive compounds. Most of them are basically biologically important. Thus the identification of a good number of compounds in various crude extracts of seaweeds and herbal plants might have potent ecological role.

Chemotaxonomy Significant in Delimitation of Six Taxa of Euphorbia L. in Iraq

Six taxa of Euphorbia L. (E. hirta L., E. milii var. milii, E. milii cv. White, E. prostrata Aiton, E. hypericifolia L. and E. serpens Kunth) collected from different location in Baghdad were extracted and the phytochemical screening of hexane crude extracts of six taxa of Euphorbia revealed that the most distributed phytochemical compounds were saponins in all taxa except E. prostrata, however, alkaloids have been reported in E. hypericifolia, E. milii cv. White and E. milii var. milii, on the other hand, glycosides have been stated in E. prostrata and E. serpens. Flavonoids and phenols have been noticed in E. serpens only, while terpenes and tannins found in E. prostata and E. hirta respectively. However, the Gas Chromatography-Mass Spectrum of methanol extract of six taxa of Euphorbia analysis explained the presence of twenty seven different chemical compounds and two new terpenoids (trans-Geranylgeraniol and Squalene) from E. milii cv. White. Agglomerative Hierarchical cluster analysis (AHC) stated close chemical relations between all the studied species, the first group comprises E. hirta and E. hypericifolia showed close relationship due to the presence of natural compounds as fatty acids, esters and aromatic alcohols However, E. prostata and E. serpens were close correlated in one group together which characterized by presence of phenols, esters and fatty acids. The anthor group contains E. milli var. milli and E. milli cv. White characterized by the abundance of di- and tri-terpenoids in addition to other natural chemical products.

Lowering polycyclic aromatic hydrocarbon concentrations in vegetable oil deodorizer distillates and enhancing its utilization by graphene composites

Vegetable oil deodorizer distillates (VODDs), which are the main source of natural tocopherol, are easily contaminated by polycyclic aromatic hydrocarbons (PAHs), and pose health threats to human beings. To remove 16 priority-control PAHs in VODD, three graphene (GR) composites, including TiO2-GR, sepiolite-GR and Fe3O4-GR, were prepared. PAH concentrations and removal rates were determined using gas chromatography-mass spectrum (GC-MS). Additionally, density functional theory (DFT) calculation was conducted to simulate and illustrate PAH adsorption on GR composites. The results indicated that the highest BaP removal rate was given to TiO2-GR with 90.37 ± 3.23 % after 2 h in standard solution. The adsorption followed the pseudo-first order kinetic, with the constant of −0.0141. In contrast, 61.62 ± 7.88 % total PAHs were removed in VODD using TiO2-GR. Moreover, DFT calculation verified that the strongest adsorption effect occurred between TiO2-GR and PAHs, with the adsorption energy of −2.69 eV. Additionally, GR composite morphology would be changed when LPAH concentrations are higher than 30 μg kg-1. The results also indicated the retention rates tocopherol ranged from 91.91 ± 1.92 % to 99.22 ± 0.88 %. This study firstly provides a useful tool for PAH removal in VODDs while retaining tocopherol. Moreover, it will increase the understanding of adsorption process between PAHs and GR composites.

Phytochemical Composition, Antioxidant, Antimicrobial, Antibiofilm, and Antiquorum Sensing Potential of Methanol Extract and Essential Oil from Acanthus polystachyus Delile (Acanthaceae).

The evolution of microbes in response to conventional antimicrobials leads to antimicrobial resistance (AMR) and multidrug resistance (MDR), and it is a global threat to public health. Natural products are possible solutions to this massive challenge. In this study, the potential of Acanthus polystachyus extracts was investigated for phytochemical composition and biological properties as antimicrobials. Gas chromatography-mass spectra (GC-MS) analysis of methanol extract (ME) and essential oil (EO) detected 79 and 20 compounds, respectively. The major compounds identified in ME and their abundance were β-sitosterol acetate (16.06%), cholest-5-en-3-yl (9Z)-9-octadecenoate (9.54%), 1-dodecanol (7.57%), (S)-(E)-(-)-4-acetoxy-1-phenyl-2-dodecen-1-one (6.03%), neophytadiene (5.7%), (E)-2-nonadecene (3.9%), hexanol-4-D2 (2.92%), and decane (2.4%). Most compounds have known bioactive functions. In EO, the major compounds were stearyl alcohol (25.38%); cis-9-tetradecenoic acid, isobutyl ester (22.95%); butyl 9-tetradecenoate (10.62%); 11,13-dimethyl-12-tetradecen-1-ol acetate (10.14%); ginsenol (3.48%); and diisooctyl phthalate (2.54%). All compounds are known to be bioactive. The antioxidant activity of ME and EO ranged from 48.3 to 84.2% radical scavenging activity (RSA) and 45.6 to 82% RSA, respectively, with dose dependency. The disc diffusion assay for the antimicrobial activity of ME revealed high inhibition against Acenetobacter baumannii (130.2%), Pseudomonas aeruginosa (100.3%), and Staphylococcus aureus (87.7%). The MIC, MBC/MFC, and MBIC values for ME were 0.5-1.0, 2-4, and 0.5-1.0 mg/mL and for EO were 0.31-0.62, 1.25-2.5, and 0.31-0.62 μL/mL, respectively, indicating inhibition potential as well as inhibition of biofilm formation. The tolerance test values indicated bactericidal activity against most strains and bacteriostatic/fungistatic activity against A. baumannii, E. faecalis, and C. albicans. The antiquorum sensing activity of ME achieved by pyocyanin inhibition assay on P. aeruginosa showed a 51.6% inhibition at 500 μg/mL. These results suggest that ME and EO derived from A. polystachyus leaves are potent, valuable, cost-effective antioxidants and antimicrobials. Both extracts may effectively combat pathogenic and resistant microbes.

Phytochemical screening, antibacterial activity, and chromatographic study of Camellia sinensis

Abstract Background: This study highlights the use of plants as a vital part of Iraq’s health care. One of the most significant plants for medicine is Camellia sinensis. Objectives: phytochemically and chromatographically evaluation of C. sinensis and its antibacterial activity. Materials and Methods: The phytochemical analysis revealed the occurrence of alkaloids, tannins, flavonoids, and phenols, but glycosides are not found in extracts of C. sinensis leaves. Antibacterial efficacy of the herbal extracts revealed that both aqueous and ethanol extracts showed some degree of inhibition of growth in Salmonella spp., Escherichia coli, Streptococcus pyogenes, Streptococcus pneumonia, and Staphylococcus aureus. Results: Nine functional groups were identified through Fourier-transform infrared spectrophotometer, and 10 phytochemical compounds were identified through gas chromatography–mass spectrum in the ethanol extract. The antibacterial activity of aqueous and ethanolic green tea extracts was tested. Both types of extracts showed clear biological activity against the studied bacterial species; the inhibitory effect increased with increasing concentration, with the highest activity of ethanolic and aqueous extracts against Salmonella spp.(inhibition zone 40, 36 mm, respectively) at 200 mg/mL, but less inhibitory effect appears on S. pyogenes when using aqueous extract (12 mm) at 50 mg/mL and (18 mm) at 200 mg/mL. Conclusions: Various therapeutically active compounds are present in the aqueous and ethanolic extract of C. sinensis, which encourages its use in the treatment of microbial infections. Further investigations are needed for the chemical composition of green tea, and other techniques like HPLC may be used. Further tests are necessary for its antimicrobial activity, either in vitro or in vivo.

Extraction of Natural Pigment Curcumin from Curcuma Longa: Spectral, DFT, Third-order Nonlinear Optical and Optical Limiting Study.

Herein, we report the extraction of natural pigment curcumin from curcuma longa and their linear and third-order nonlinear optical (NLO) characteristics. The characterization techniques viz., UV-Visible absorption, FT-IR, Micro Raman and Gas Chromatography Mass Spectrum (GC-MS) are used to study the spectral characteristics of curcumin.Third-order NLO features of curcumin are studied using Z‒scan technique with a semiconductor diode laser working at 405nm wavelength. The natural pigment exhibits negative nonlinear index of refraction resulting from self-defocusing and positive coefficient of absorption is the consequence of reverse saturable absorption (RSA). The order of nonlinear index of refraction (n2) and nonlinear coefficient of absorption (β) is measured to be 10-7 cm2/W and 10-2cm/W, respectively. Third-order NLO susceptibility (χ(3)) and second-order hyperpolarizability (γ) of curcumin is measured to be 2.73 × 10‒7 esu and 1.67 × 10‒31 esu, respectively. A low optical limiting (OL) threshold of 0.71 mW is observed in the extracted pigment. The experimental results are supplemented by quantum mechanical calculations of the NLO parameters. The overall result finding is that curcumin extracted from curcuma longa has the potential to be novel optical candidates for photonics and optoelectronics applications.

Evaluation of Antibacterial and Antibiofilm Activity of Cucurbita Maxima Leaf Extract Against Streptococcus Mutans Isolated from Orthodontic Patients

Aim The aim of this investigation was to evaluate the antimicrobial activity and anti-biofilm activity of Cucurbita Maxima leaf extract on Streptococcus mutans isolated from dental plaque of subjects undergoing orthodontic treatment. Methodology Dental plaque samples were collected from out patients undergoing orthodontic treatment. From the plaque samples Biochemical Characterisation of Streptococcus mutans then Collection of Medicinal Plants was done followed by Preparation of Solvent Extract and stock Solution. Evaluation of Minimum Inhibitory Concentration (MIC) of the plant extract and Biofilm Inhibition Assay with Gas Chromatography-Mass Spectrum Analysis (GCMS) was carried out. Results The MIC was identified to be 1.25mg/ml. The biofilm inhibition assay showed that the extract did not inhibit the formation of the biofilm. The GCMS analysis identified twenty five constituent compounds from the crude extract. Conclusion The C. maxima leaf extract showed antibacterial activity against S. mutans and the minimum inhibitory concentration was identified at 1.25mg/ml. The extract did not inhibit the formation of biofilm.

Chemical analysis of novel ginger and Hibiscus sabdariffa bio-preserved tomato powder

Background: Tomatoes (Solanum lycopersicum L.) are widely consumed vegetables globally, and are well known for their nutrients and economic value. This research aimed to create a tomato powder with improved nutraceutical benefits based on its ingredients. Methods: Fresh tomatoes, ginger, and dried hibiscus calyx were obtained and dehydrated at 45°C for 10 hours, 8 hours, and 1 hour and 30 minutes respectively. Proximate, elemental, phytochemical, high-performance liquid chromatography (HPLC), and gas chromatography-mass spectrum (GCMS) analysis of the tomato powder mix were determined using standard methods.Results: The proximate analysis revealed constituents of carbohydrates, fats, proteins, ash, fiber, and moisture as 65, 5, 10, 1, 7, and 12 % respectively. The GCMS also revealed 36 classes of compounds with different retention times. The most prominent peak is oleic acid with an RT value of 20.9. The fiber content of the tomato powder mix was higher than what is obtainable in fresh tomatoes. Bioactive compounds like stigmasterol (5.25%), oleic acids (49.11%), n-hexadecenoic acid (26.58%), campesterol (2.72%), and octadecanoic acid (6.8%) are notably present with lycopene (135.745 mg/100 grams). Conclusion:The tomato powder mix is nutrient-packed and non-toxic. The bioactive components are known as antioxidant, anti-inflammatory, cardiovascular health enhancers, etc showing the possible use of the powder as a functional food.Keywords: Tomatoes powder, bioactive compounds, functional foods, dehydration

Potential Antibacterial Activity of Milkfish (Chanos chanos) Bile Extract against Escherichia coli and Staphylococcus aureus

Fish is one of the popular health foods worldwide and has a huge medicinal importance. Several traditional medicinal practitioners claimed that bile content of fresh water fish may useful as antibiotic. Therefore, the present work was undertaken to evaluate the antibacterial activity of the bile content of Milkfish (Chanos chanos). The study manifested that the Milkfish bile extract was partially active against partially active on S. aureus and inactive on E. coli. There were no significant differences on the antibacterial activity among the three concentrations of Milkfish bile extract and the control drug against the two bacterial pathogens (E. coli and S. aureus). The results of this study have shown that bile contains some substances that can exert inhibitory effect on bacteria hence can be employed in the treatment of diseases caused by these groups of bacterial pathogens. To provide a more conclusive and convincing claim on the effect of Milkfish bile extract, it is recommended that Quantitative analysis be conducted using gas chromatography mass spectrum of the bile extract to accurately measure the different components; Further research studies can be continued to discover other wonder properties of this fish in promoting good health as an alternative medicine; and the use of other fishes may be investigated to determine the presence of bioactive components.

2D hydroxylated MXene (OH-MXene)/RGO composites modification toward superior electrocatalytic degradation of paracetamol

The MXene precursor was adjusted with a combination of –OH grafting and reduced graphene oxide (RGO) via alkalization and hydrothermal method to improve the electrocatalytic performance. The as-prepared hydroxylated MXene (OH-MXene)/RGO composites possess maximum 100 % paracetamol (APAP) degradation efficiency under an applied current of 15 mA, temperature of 25 °C, and electrolyte of 1 g/L NaCl. All characterization and experiment results showed that the grafting of –OH groups into MXene could access the active site of Ti-OH, strengthen the linking of OH-MXene and RGO layers (as C-Ti-Ox), accelerate the free electrons transfer from OH-MXene to RGO; thus, enhancing the catalytic activity of OH-MXene/RGO composites. Then, •OH, 1O2, O2•−, and active chlorine were determined as secondary active species in the solution, which dominated the APAP degradation. Three degradation pathways of APAP were further proven by gas chromatography-mass spectrum (GC–MS) with cleavage of N-branched chain and benzene rings. In addition, the superior and stable performance of OH-MG-15h under various operation conditions confirmed the application perspective of self-prepared material. The research not only provides feasible methods for MXene adjustment and electrocatalytic activity improvement but also proves its possibility for environmental application.