A high pressure cold spray system was used to deposit three Ti-6Al-4V feedstock powders (i.e., hydride de-hydride, plasma atomized, and gas atomized) on Ti-6Al-4V substrates while varying gas temperature and nozzle length. Particle impact temperature and particle velocity were calculated using a 1-D axial model. The microstructure of the feedstock powders and the cold spray depositions were characterized via optical and scanning electron microscopy. The hardness of the as-received powders was determined using nanoindentation. To assess deposition quality, coatings were characterized in terms of porosity, microhardness, and adhesion strength. Results showed that hydride de-hydride powders were characterized by an equiaxed alpha grain structure with intergranular beta phase regions while atomized powders were characterized by martensitic α phase structures. Cold sprayed coatings revealed two distinct microstructures. Regions that experienced low/moderate plastic strain retained the as-received powder microstructure while regions that experienced significant plastic strain were characterized either by a featureless microstructure (atomized coatings) or the presence of fine, elongated beta precipitates (hydride de-hydride coatings). Depositions performed using a long nozzle resulted in the best deposition quality, with porosity as low as 0.3% and adhesion strengths >69MPa. While atomized powders resulted in comparatively higher quality coatings for all process conditions, hydride de-hydride coatings of excellent quality (average porosity≈0.6%, adhesion strength >65MPa) were achieved under optimal conditions. Thus, hydride de-hydride powders may hold promise as a cost effective alternative to atomized powders for Ti-6Al-4V cold spray depositions.