Present study deals with synthesizing CuNPs from Garlic (Allium sativum) plants leaf and investigates its antibacterial activity against different bacteria. CuNPs was synthesized by Herbal method. The characterization of nanoparticles was done by XRD, FTIR and UV-spectroscopy method. Antibacterial activity was determined by disc diffusion, well diffusion method and MIC. This green synthesis method is alternative to chemical methods, since it is cheap, pollutant free and eco-friendly. Thus the obtained CuNPs demonstrated remarkable antibacterial activity against three human pathogenic bacteria when used in combination with commercially available antibiotics. CuNPs have become an important approach for applications in nanobiotechnology in the development of antibiotic treatment of different bacterial infections.