Galium Aparine Research Articles

Protection of grain crops with herbicide Secator Plus, MD

The natural and climatic conditions of Belarusare favorable for development of over 300 species of weeds. Currently, perennial, wintering, winter and spring weeds dominate among grain crops. When grain crops are infested with both annual and perennial dicotyledonous weeds, herbicides containing several active substances are efficient. One of such promising herbicides is the new herbicide Secator Plus, MD(2.4 acid, 433 g/l + iodosulfuron, 62.5 g/l + amidosulfuron, 25 g/l + mefenpyr /antidote/, 62.5 g/l). This herbicide used at the rate of 0.3-0.5 l/ha for winter grain crops provided high biological efficiency against the dominant annual and some perennial dicotyledonous weeds (80–100% loss): Chenopodium album L., Capsella bursa-pastoris (L.) Medik., Galeopsis tetrahit L., Tripleurospermum inodorum Sch.-Bip., Galium aparine L., Sonchus arvensis L., etc. In the studied crops, reliable increases in preserved grain yield were obtained. Economic benefit: the net income in winter wheat crops amounted to 60.0-90.0 dollars/ha, winter triticale 35.5-43.7 dollars/ha, and spring wheat 159.9-212.3 dollars/ha. Based on the research results, the herbicide Secator Plus, MD is registered in the State Register of Plant Protection Products (pesticides) and fertilizers permitted for use on the territory of theRepublic ofBelarus. The research results are of interest in the field of herbology, since they reveal the mechanisms of interaction of various active substances in herbicide, show possibilities of its widespread use in agriculture.

The effect of vegetation in apple orchard edges on the phenology of parasitoids from the subfamily Pimplinae (Hymenoptera, Ichneumonidae)

Wild vegetation neighbouring orchards may be a factor attracting imagines of parasitoids from the subfamily Pimplinae into fruit tree plantations and thus increase both their species diversity and population size in this habitat. For this reason in the years 2008–2010 a study was initiated on the phenology of 8 dominant Pimplinae species in apple orchards and on their edges, which included shrubberies and roadside avenues of trees and shrubs. Slightly higher numbers of Pimplinae were recorded in orchards compared to their edges. At strong correlation was observed between the counts of Pimplinae in both habitats. The preference of selection of orchards by Pimplinae was observed in the autumn period, while no such preference was found in the spring or summer months. Analyses showed that flowering plants in the orchard edges such as Tilia cordata, Symphoricarpos albus, Cirsium arvense and Galium aparine may have attracted Pimplinae to the orchards.

Ethnobotanical survey of the medicinal flora of Harighal, Azad Jammu & Kashmir, Pakistan

BackgroundThe present study is the first quantitative ethnobotanical evaluation of Harighal, an inaccessible and unexplored area of District Bagh Azad Jammu & Kashmir (AJK). The exploration, quantification, and comparison of ethnobotanical knowledge among different rural communities of the study area were mainly focused during field survey.MethodologyIn total, 79 informants (49 men and 34 women) were selected randomly to collect data using a semi-structured questionnaire. Various quantitative indices, including use value, relative frequency of citation, relative importance, fidelity level, and informant consent factor, were employed to evaluate the gathered information. Furthermore, primary data were also compared with twenty-two papers published from adjoining areas.ResultA total of 150 medicinal plants belonging to 98 genera and 60 families were documented. Asteraceae, Fabaceae, and Rosaceae were the dominant families having 15 species each. Of these, 76 species were indigenous, 74 exotic, 136 were collected in the wild, 10 cultivated, and 4 both wild-collected and cultivated. Herbaceous taxa were the most used life form, and leaves were the most exploited plant part. Decoctions were the most preferred method used in preparation of herbal recipes. Three species viz. Mentha longifolia, Berberis lycium, and Galium aparine had the highest use value (1.05), relative frequency of citation (0.81), and relative importance value (96), respectively. The highest informant consensus factor (ICF) was reported for digestive disorders. Mentha longifolia, Punica granatum, Zanthoxylum alatum, and Olea ferruginea had 100% fidelity values. The Jaccard index revealed that uses of plants were more similar in two neighboring areas, i.e., Pearl Valley and Toli Peer.ConclusionLocal inhabitants still prioritize herbal medicines as an effective way to treat a wide variety of ailments. Elders and health practitioners of the study area are well aware of indigenous knowledge about medicinal plants, but young people are not much interested in herbal practices. Thus, valuable knowledge about the use of plants is on the verge of decline.

Selectivity and efficacy of herbicide GF-3488, containing synthetic auxins halauxifen-methyl and clopyralid, in winter oilseed rape crops in Ukraine

Halauxifen-methyl is a new synthetic auxin, which in combination with another auxin-like herbicide clopyralid is the active ingredient of complex herbicide GF-3488, that is used for dicotyledonous weeds control in winter oilseed rape crops. The reason of this study was a necessity to test crop selectivity and efficacy of GF-3488 to control dicotyledonous weeds in winter oilseed rape crops in Ukraine and also the possibility of GF-3488 application in the tank mixture with graminicide or/and insecticide to control monocotyledonous weeds and insects. A randomized block experiment was conducted in 2015—2017 in 4 locations. It was found that under spring applying, the GF-3488 is not inferior to another widely used herbicide Galera Super on selectivity to the crop. Herbicide GF-3488 at the rate 1.0 l/ha was significantly better than Galera Super at the rate 0.3 l/ha in controlling of annual dicotyledonous weeds Papaver rhoeas L., Galium aparine L., Consolida regalis S.F. Gray and was effective to control of certain types of cruciferous weeds, which are resistant to Galera Super. Adding graminicide Fusilade Forte and insecticide Nurelle D did not affect the GF-3488 selectivity to the crop and had no negative impact on dicotyledonous weeds control. Efficacy of monocotyledonous weeds control by graminicide did not change in the mixture with GF-3488. It was concluded that herbicide GF-3488 is effective on winter oilseed rape against dicotyledonous weed species. Therefore for the simultaneous control of dicotyledonous and monocotyledonous weeds in winter oilseed rape in the spring after the renewal of the crop vegetation herbicide GF-3488 is advisable to be used in a tank mixture with graminicides, and for simultaneous protection against weeds and pests it can be mixed with insecticides.

If phenolic compounds in the soil with buckwheat residues affect the emergence and growth of weed seedlings?

This study was conducted to determine if common buckwheat (Fagopyrum esculentum Moench) residues affect a phenolics composition in soil, and are effective for limiting emergence, growth and metabolic changes in barnyard grass (Echinochloa crus-galli (L.) P. Beauv.), wild oat (Avena fatua L.), yellow foxtail (Setaria pumila Schult.), silky windgrass (Apera spica-venti L.), catchweed bedstraw (Galium aparine L.), scentless mayweed (Matricaria inodora L.), and tiny vetch (Vicia hirsuta L.). In the study, the residues of 14-day-old buckwheat plants were used (cv. Hruszowska). After removal of the above-ground parts, the buckwheat root residues (BRR) remained in the soil for an additional 7 days before the weeds were sown. For comparison, under the same cultivation conditions, the effect of entire buckwheat plant residues (BPR) in soil was assessed. BPR and BRR in the soil caused a decrease in the emergence of all weed species except the tiny vetch. The growth of barnyard grass, wild oat, yellow foxtail, catchweed bedstraw, and scentless mayweed was inhibited by BRR, but not BPR. BRR had a particularly strong inhibitory effect on the growth of scentless mayweed (SM) and catchweed bedstraw (CB). Thirty-day-old SM and CB control plants were about 4 and 3.5 times higher, respectively, than plants growing in the presence of BRR. Furthermore, chlorophyll and carotenoid contents in the barnyard grass and catchweed bedstraw were more prominently reduced by BRR than BPR. Stressful conditions caused by buckwheat residues in the soil increased the level of phenolic compounds in the tissues of weeds examined. Soil with buckwheat residues contained a low level of phenolic compounds, which may indicate their slow release from the residue or rapid utilisation by microorganisms. These phenolic compounds probably cannot be directly responsible for allelopathic inhibition of weed emergence and growth.

Climbing Plant‐Inspired Micropatterned Devices for Reversible Attachment

AbstractClimbing plants have evolved over millions of years and have adapted to unpredictable scenarios in unique ways. These crucial features make plants an outstanding biological model for scientists and engineers. Inspired by the ratchet‐like attachment mechanism of the hook‐climber Galium aparine, a novel micropatterned flexible mechanical interlocker is fabricated using a 3D direct laser lithography technique. The artificial hooks are designed based on a morphometric analysis of natural hooks. They are characterized in terms of pull‐off and shear forces, both in an array and as individual hooks. The microprinted hooks array shows high values of pull‐off forces (up to F⊥ ≈ 0.4 N cm−2) and shear forces (up to F// ≈ 13.8 N cm−2) on several rough surfaces (i.e., abrasive materials, fabrics, and artificial skin tissues). The contact separation forces of individual artificial hooks are estimated when loads with different orientations are applied (up to F ≈ 0.26 N). In addition, a patterned tape with directional microhooks is integrated into a mobile platform to demonstrate its climbing ability on inclined surfaces of up to 45°. This research opens up new opportunities for prototyping the next generation of mechanical interlockers, particularly for soft‐ and microrobotics, the textile industry, and biomedical fields.

Effects of intercropping accumulator plants and applying their straw on the growth and cadmium accumulation of Brassica chinensis L.

Two pot experiments were conducted to study the effects of intercropping cadmium (Cd) accumulator plants (Stellaria media (L.) Villars, Cardamine hirsuta, Cerastium glomeratum Thuill, and Galium aparine L.) and applying their straw on the growth and Cd accumulation of Brassica chinensis L. Intercropping with four accumulator plants reduced the biomass, water content, and photosynthetic pigment content of B. chinensis compared with monoculture. Intercropping with accumulator plants increased the Cd content in the roots and shoot of B. chinensis, and the translocation factor (TF), root bioconcentration factor (root BCF), and shoot bioconcentration factor (Shoot BCF) increased. The soil pH decreased and the soil available Cd content increased by intercropping. Thus, intercropping with four accumulator plants can promote the Cd uptake of B. chinensis. The straw of four accumulator plants reduced the biomass, water content, and photosynthetic pigment content of B. chinensis compared with the control. The straw of S. media and C. hirsute increased the Cd content in the roots and shoots of B. chinensis, TF, root BCF, and shoot BCF. The straw of C. glomeratum and G. aparine decreased the Cd content in the roots and shoots of B. chinensis, TF, root BCF, and shoot BCF. The soil pH increased and the soil available Cd content decreased by application of straw. Thus, the straw of C. glomeratum and G. aparine can reduce the Cd uptake of B. chinensis.

Potential of controlling selected weeds in Europe with insects

Weed control by insects is increasingly important, as chemical weed control (the use of herbicides) has an important impact on the environment and, consequently, on all organisms living there. The use of insects to control weeds thus represents an alternative to herbicides. The article presents the suppression of some widespread and persistent weeds in Europe with their natural enemies - insects. The following combinations presented below are: broad-leaved dock (Rumex obtusifolius L.) – Gastrophysa viridula (De Geer, 1775), curly dock (Rumex crispus L.) – Apion violaceum (Kirby, 1808), common ragweed (Ambrosia artemisiifolia L.) – Ophraella communa (LeSage, 1986) and Zygogramma suturalis (Fabricius, 1775), creeping thistle (Cirsium arvense (L.) Scop.) – Cassida rubiginosa (Müller, 1776), cleavers (Galium aparine L.) – Halidamia affinis (Fallen, 1807) and Sermylassa halensis (Linnaeus, 1767), common knotgrass (Polygonum aviculare L.) and black-bindweed (Fallopia convolvulus L.) – Gastrophysa polygoni (Linnaeus, 1758) and as the last one field bindweed (Convolvulus arvensis L.) – Galeruca rufa (Germar, 1824) and Tyta luctuosa (Denis in Schiffmuller, 1775).

The effects of soil tillage techniques on weed flora in high input barley systems in northern Spain

In barley cropping systems of northern Spain, agronomic practices and weather conditions are key components of weed control efficacy. We compared the short-term effects of conventional tillage with minimum tillage (MT) and zero tillage (ZT), in barley monoculture and barley rotation systems. Weed density and weed species number were measured at tillering and flowering barley stages. We found that tillage system can influence weed density and weed species establishment due to, in part, the available light for weed seeds. The results obtained indicate that the MT system facilitates the prevalence of the grass weed Bromus diandrus Roth (50.37%) and the annual dicots Galium aparine (L.) and Buglossoides arvensis (L.) I.M. Johnst. abundant were high in the MT system too, 43.71% and 43.97% respectively. The germination of these species showed a high dependence on light availability. We saw that barley-monoculture plots had a large infestation of Bromus (71.29%) and barley-rotated plots presented more infestation of Galium and Buglossoides (74.36% and 84.4%, respectively). After herbicide application, weed infestation in conservation systems was reduced in barley-rotated plots compared with barley-monoculture. If conservation systems avoided the presence of dominant weeds, the weight of each weed species was balanced within competitive relationships of the cropping systems. Our results confirmed that MT and ZT systems favour different weed species emergences in barley-rotated plots. The combination of MT and barley-rotated cropping system resulted in greater weed diversity and lower total weed density.

Impact of River Water and Bottom Sediment Pollution on Accumulation of Metal(loid)s and Arsenic Species in the Coastal Plants Stuckenia pectinata L., Galium aparine L., and Urtica dioica L.: A Chemometric and Environmental Study

The role of water and bottom sediment pollution of a river subjected to a strong industrial anthropo-pressure in coastal plants was investigated. The work presented the influence of polluted environment on accumulation of metal(loid)s (including arsenic and its species) in Stuckenia pectinata L., Galium aparine L., and Urtica dioica L. The study provided important information on the contents of organic and inorganic arsenic species in selected plants and their response to heavy metal and arsenic contamination. The As(III), As(V), AB (arsenobetaine), MMA (monomethylarsonic acid), and DMA (dimethylarsinic acid) ions were successfully separated on the Hamilton PRP-X100 column with high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) techniques. The Pollution Load Index and geo-accumulation Index (Igeo) values clearly indicate significant pollution of the examined ecosystem with heavy metals. The chemometric analysis with the concepts of (Dis)similarity Analysis, Cluster Analysis, and Principal Component Analysis helped to visualize the variability of the As species concentrations and to analyse correlations between sampling point locations and analyte contents.

Vascular plants of Victoria Island (Northwest Territories and Nunavut, Canada): a specimen-based study of an Arctic flora

Victoria Island in Canada’s western Arctic is the eighth largest island in the world and the second largest in Canada. Here, we report the results of a floristic study of vascular plant diversity of Victoria Island. The study is based on a specimen-based dataset comprising 7031 unique collections from the island, including some 2870 new collections gathered between 2008 and 2019 by the authors and nearly 1000 specimens variously gathered by N. Polunin (in 1947), M. Oldenburg (1940s–1950s) and S. Edlund (1980s) that, until recently, were part of the unprocessed backlog of the National Herbarium of Canada and unavailable to researchers. Results are presented in an annotated checklist, including keys and distribution maps for all taxa, citation of specimens, comments on taxonomy, distribution and the history of documentation of taxa across the island, and photographs for a subset of taxa. The vascular plant flora of Victoria Island comprises 38 families, 108 genera, 272 species, and 17 additional taxa. Of the 289 taxa known on the island, 237 are recorded from the Northwest Territories portion of the island and 277 from the Nunavut part. Thirty-nine taxa are known on the island from a single collection, seven from two collections and three from three collections. Twenty-one taxa in eight families are newly recorded for the flora of Victoria Island: Artemisia tilesii, Senecio lugens, Taraxacum scopulorum (Asteraceae); Crucihimalaya bursifolia, Draba fladnizensis, D. juvenilis, D. pilosa, D. simmonsii (Brassicaceae); Carex bigelowii subsp. bigelowii, Eriophorum russeolum subsp. albidum (Cyperaceae); Anthoxanthum monticola subsp. monticola, Bromus pumpellianus, Deschampsia cespitosa subsp. cespitosa, D. sukatschewii, Festuca rubra subsp. rubra, Lolium perenne, Poa pratensis subsp. pratensis (Poaceae); Stuckenia filiformis (Potamogetonaceae); Potentilla × prostrata (Rosaceae); Galium aparine (Rubiaceae); and Salix ovalifolia var. ovalifolia (Salicaceae). Eight of these are new to the flora of the Canadian Arctic Archipelago: Senecio lugens, Draba juvenilis, D. pilosa, Anthoxanthum monticola subsp. monticola, Bromus pumpellianus, Deschampsia cespitosa subsp. cespitosa, Poa pratensis subsp. pratensis and Salix ovalifolia var. ovalifolia. One of these, Galium aparine, is newly recorded for the flora of Nunavut. Four first records for Victoria Island are introduced plants discovered in Cambridge Bay in 2017: three grasses (Festuca rubra subsp. rubra, Lolium perenne, and Poa pratensis subsp. pratensis) and Galium aparine. One taxon, Juncus arcticus subsp. arcticus, is newly recorded from the Northwest Territories. Of the general areas on Victoria Island that have been botanically explored the most, the greatest diversity of vascular plants is recorded in Ulukhaktok (194 taxa) and the next most diverse area is Cambridge Bay (183 taxa). The floristic data presented here represent a new baseline on which continued exploration of the vascular flora of Victoria Island – particularly the numerous areas of the island that remain unexplored or poorly explored botanically – will build.

Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.

To accurately evaluate expression levels of target genes, stable internal reference genes is required for normalization of quantitative real-time PCR (qRT-PCR) data. However, there have been no systematical investigation on the stability of reference genes used in the bedstraw weed, Galium aparine L. (BGA). In this study, the expression profiles of seven traditionally used reference genes, namely 18S, 28S, ACT, GAPDH, EF1α, RPL7 and TBP in BGA were assessed under both biotic (developmental time and tissue), and abiotic (temperature, regions and herbicide) conditions. Four analytical algorithms (geNorm, Normfinder, BestKeeper and the ΔCt method) were used to analyze the suitability of these genes as internal reference genes. RefFinder, a comprehensive analytical software, was used to rank the overall stability of the candidate genes. The optimal normalization internal control genes were ranked as: 28S and RPL7 were best for all the different experimental conditions (developmental stages, tissues, temperature, regions and herbicide treatment); 28S and RPL7 for developmental stages; TBP and GAPDH for different tissues; 28S and GAPDH were relatively stable for different temperature; 28S and TBP were suitable for herbicide treatment. A specific set of reference genes were recommended for each experimental condition in BGA.

Allelopathic effect of buckwheat extract for seedlings of selected weed species

The influence of 1% aqueous extract obtained from a 14-day plants of common buckwheat (Fagopyrum esculentum Moench) on weed species was evaluated. Seedling roots of wild oat (Avena fatua L.), yellow foxtail (Setaria glauca L.), barnyardgrass (Echinochloa crus galli (L.) P. Beauv.), common windgrass (Apera spica-venti (L.) P. Beauv.), catchweed bedstraw (Galium aparine L.), scentless mayweed (Matricaria inodora L), gallant soldier (Galinsoga parviflora Cav.) and tiny vetch (Vicia hirsuta L.) were exposed to buckwheat extract and compared with control plants grown in water. The obtained results show that the buckwheat extract had lower influence on the growth of shoot than on roots of the evaluated weed species. The use of buckwheat extract in the medium caused the inhibition of root growth in all species except from tiny vetch. In the case of shoot of weeds, inhibition of growth by buckwheat extract occurred only in wild oat. Whereas in yellow foxtail, scentless mayweed and tiny vetch, the stimulation of shoot growth was demonstrated. These results may indicate that the buckwheat extracts act on directly exposed tissues. A different metabolic reaction of wild oat to buckwheat extract after 5 days of exposure than after 2 days may indicate a quick adaptation of wild oat seedlings to stressful conditions.

A Study on Weed Flora and Importance Value Index of Weeds in Wheat Crop

Weeds are an important problem because they reduce the yield and quality of wheat. This study was carried out to determine the phytosociology of weeds in wheat fields of Siirt, Turkey. A square-frame inventory method was used that was randomly released at least four times from 90 different locations between March and May in 2019. A total of 71 weed species belonging to 20 different families, one of which is grass (12 species) and 19 broadleaf (59 species) were identified. Asteraceae (with 15 species) were identified as the predominant family, followed by Poaceae (with 12 species), Brassicaceae and Fabaceae (with 8 species). The highest frequency, density and abundance values were determined Avena sterilis L. (83%, 11.4 plant m-2, 32.8%), Sinapis arvensis L. (81%, 9.6 plant m-2, 41.3%), Ranunculus arvensis L. (77%, 4.3 plant m-2, 24.4%), Galium aparine L. (66%, 8.2 plant m-2, 12.8%), and Cirsium arvense (L.) Scop. (61%, 5.5 plant m-2, 29.0%) species were the maximum. When the above values are calculated relatively, importance value index (IVI) were detected Avena sterilis L. (22.3%), Sinapis arvensis L. (22.2%), Papaver rhoeas L. (15.9%), Cirsium arvense (L.) Scop. (14.1%), and Galium aparine L. (13.5%) species were the highest. According to the economic threshold (according to the current literature) Avena sterilis L. and Sinapis arvensis L. were identified as noxious weeds. This research will shed light on the determination of the noxious weeds, economic threshold and environmentally-friendly integrated weed management tactics to be developed in wheat. Keywords: Wheat, Weeds, Frequency, Density, Abundance, Importance value index DOI: 10.7176/JSTR/6-01-05

БИОТОПИЧЕСКИЕ ОСОБЕННОСТИ РАСПРОСТРАНЕНИЯ И УСТОЙЧИВОСТЬ К АНТРОПОГЕННОМУ ВОЗДЕЙСТВИЮ LISTERA OVATA (L.) R.BR. В КИРОВСКОЙ ОБЛАСТИ

Stash ovate (Listera ovata (L.) R. Br.) – herbaceous short-rooted perennial. The purpose of the study is to identify the environmental preferences of Listera ovata and its resistance to anthropogenic impact on the territory of the Kirov region. As a result of phytoindication of the studied Listera ovata habitats, ecological characteristics were obtained using six H. Ellenberg scales (1974). According to the illumination scale, which evaluates the ratio of plants to the relative illumination prevailing in the habitat of the species, Listera ovata grows at different levels of illumination: as in open habitats with 60–80 % illumination. In relation to the thermoclimatic factor that evaluates heat / cold resistance, the studied species is an inhabitant of a temperate climate (the 5th stage of the Ellenberg scale). On the continental scale Listera ovata is characterized as a suboceanic species (4th stage of the Ellenberg scale), which corresponds to the geographical distribution zone of the species within the Kirov region. In relation to soil moisture, the distribution of the species in habitats with soils ranging from medium-wet (5th stage of the Ellenberg scale) to wet (6th stage of the Ellenberg scale) was noted. On the scale of soil acidity, which determines the dependence of species on acid-base soil conditions, Listera ovata occupies habitats characterized from moderately acidic (5th stage of the Ellenberg scale) to weakly alkaline soils (7th stage of the Ellenberg scale). According to the nitrogen richness scale, which shows the total supply of nutrients (N, K, P, Mg) in the soil, the species is confined to habitats with moderately provided basic nutrients (5th stage of the Ellenberg scale). Analysis of hemerobicity by species composition in plant communities showed that the studied plant communities with Listera ovata are mainly represented by oligo and meso-hemerobic species: from 30.56 to 37.78 %. These are species of communities that are close to natural, that tolerate irregular weak influences, and species of semi-natural communities that are resistant to extensive influences (Vaccinium vitis-idaea, Fragaria vesca, Asarum europaeum, Pimpinella saxifraga, Melica nutans, Maianthemum bifolium, Vaccinium myrtillus). The share of species of natural communities that do not tolerate anthropogenic influence is 1.65 %. About 21 % are occupied by species far from natural communities that are resistant to intensive use (Melilotus albus, Pastinaca silvestris, Calamagrostis epigeios). Weed species of natural and anthropogenic communities that suffer regular severe disturbances account for 7.67 % (Trifolium repens, Galium odorata, Taraxacum officinale). The share of specialized weed species of intensive crops is 4.06 % (Chamaenerion angustifolium, Tussilago farfara, Cirsium arvense, Galium aparine, Anthriscus sylvestris). Types of completely disturbed ecosystems in the studied habitats were not identified. All studied phytocenoses with L. ovata are dominated by anthropophobic species: from 62 to 77 %. The percentage of anthropotolerant species is not high and varies from 23 to 38 %.

Адвентивные виды во флоре Верхнего Енисея

In the course of field research, analysis of previously published works and viewing materials stored in the herbarium of the Central Siberian Botanical Garden SB RAS named after I. M. Krasnoborov (NS) we clarified an information on the distribution of rare adventive plant species in the territory of the Upper Yenisei basin. Cosmos bipinnatus, Ipomoea hederacea, Setaria maximowiczii and Sonchus oleraceus were noted for the first time in the Tuva flora, Grossularia reclinata in the Western Sayan, and Polygonum calcatum and Spergula vulgaris in the territory of Khakassia. Echinochloa crusgalli, Fagopyrum esculentum, Fragaria ananassa, Galium mollugo and Grossularia reclinata were new to the flora of the Sayano-Shushensky nature reserve. For 14 rare species new localities were noted and the boundaries of the ranges were specified: Amaranthus albus, Carduus nutans, Echinochloa crusgalli, Epilobium adenocaulon, Galium aparine, G. mollugo, Hordeum jubatum, Lactuca serriola, Oenothera biennis, Portulaca oleracea, Psammophilliella muralis, Setaria faberi, Sisymbrium loeselii, Spergula vulgaris.

Herbicidal activity of Aureobasidium pullulans PA-2 on weeds and optimization of its solid-state fermentation conditions

Fungal strain PA-2 was isolated from infected poplar leaves from the Ping'an District, Haidong City of Qinghai Province, China. Based on the culture characteristics and the internal transcribed spacer (ITS) sequence of its 16S rDNA, the strain was identified as Aureobasidium pullulans. The culture and metabolites of strain PA-2 showed high herbicidal potential to five tested weeds Galium aparine var. tenerum, Chenopodium album, Malva crispa, Polygonum lapathifolium and Avena fatua. For the in vitro test, 5 days after the detached leaves were inoculated with PA-2 culture, all leaves infected by the hyphae and became black and rotten. For the in vivo test, a metabolite filtrate of PA-2 culture was sprayed over the living weed plants, and five days after inoculation, the weed plants became withered and necrotic. Seven days after inoculation, the fresh weight reductions of G. aparine var. tenerum, C. album, M. crispa, P. lapathifolium and A. fatua were 87.25, 78.46, 82.25, 62.11, and 80.27%, respectively. Galium aparine var. tenerum and M. crispa exhibited significant reductions in fresh weight. The bio-safety test on the five crops showed no significant plant height reductions, which was also observed for wheat (Triticum aestivum), faba bean (Vicia faba), and barley (Hordeum vulgare). By contrast, oilseed rape (Brassica napus) and pea (Pisum sativum) exhibited light spots but no significant reductions in plant height. These results indicated that A. pullulans could be a potential microbial herbicide for the control of the target weeds in crops. Optimization of the carbon and nitrogen sources for cultural media and substances for solid-state fermentation indicated that PA-2 had better colony growth and spore production with the optimal carbon source of glucose (C4), nitrogen source of soybean flour (N2), and the optimal substance was wheat bran. The results in this study provide useful information for the development of A. pulluans PA-2 as an herbicide for bio-control of the weed.

Вплив екологічних чинників на сегетальну рослинність в агроценозах зернових культур Правобережного Лісостепу України

The determination of the influence of various e cological factors on the p roperties of the development of segetal vegetation in the cultural plants agrocenoses is important for the develop ment of the effective and environmentally friendly systems. From the standpoint of ecological safety of agroecosystems and the quality of agricultural products, modern agrotechnologies should be based on integrated methods of plant protection with the accounting of climate change, the rational use of chemical methods with minimal negative effect on the environment. It was established that under the conditions of the R ight- B ank F orest- S teppe in agrocenoses of grain crops (winter wheat, oats), 8 species of weeds prevail, which are arranged in a row in terms of population density: field bindweed (Convolvulus arvensis (L.) Scop.), bedstraw (Galium aparine L.), manure weed (Chenopodium album L.), creeping thistle (Cirsium arvense ( L.) Scop.), quickweed (Galinsog parviflora Cav.), shepherd's purse (Capsella bursa-pastoris (L.) Medik.), amaranth (Amaranthus retroflexus L.), wild radish (Raphanus raphanistrum L.). It was determined that with an excess of moisture ( MPCs 1.73, 1.76), the level of pollution by agrocenosis in the phase of spring tillering was on average 9 - 40 % higher than with strong and dry ( MPCs 0.52, 0.87; MPCs 0.15, 0.83). At an average level of weeds in crops, the insurance herbicide Prima is applied, s.e. (0.4 l/ha), Starana Premium 330 EU, e.c. (0.3 l/ha) and Granstar Pro 75, w.s.g. (20 g/ha) with the minimum recommended consumption rates, an increase in the yield of winter wheat by 25.4–27.3%, oats – by 25.9–27.2%. The technical efficiency of herbicides was at the level of 87.6–92.6%, and the economic efficiency was 26–27%. The use of these herbicides for a single treatment of crops in the phase of spring tillering can be recommended in the integrated protection system for winter wheat and oats in the conditions of the Right-Bank Forest-Steppe.

Analiza olejku eterycznego z Galium aparine.

Analiza olejku eterycznego z Galium aparine.

Determination of Segetal Flora in Wheat Fields in Iğdır Province and Investigation of Growth Biology of Some Weeds Species

Bu çalışma Iğdır ili buğday ekim alanlarındaki yabancı ot türlerini, yoğunluklarını, rastlanma sıklıklarını, kaplama alanlarını belirlemek ve önemli bazı yabancı otların gelişme biyolojilerini incelemek amacı ile 2017-2018 üretim döneminde yürütülmüştür. Bu amaçla Mayıs-Haziran aylarında Iğdır Merkez, Aralık, Karakoyunlu ve Tuzluca ilçelerinde buğday ekim alanları göz önünde bulundurularak sürveyler gerçekleştirilmiştir. Sürveylerde dört adet 1m2’lik çerçeveler tesadüfi olarak tarlanın içine atılmış çerçeve içerisine denk gelen yapancı ot türlerinin rastlama sıklıkları (%), yoğunlukları (adet/m2) ve kaplama alanları (%) belirlenmiştir. Sahip oldukları yabancı ot tür sayılarına göre en geniş üç familya sırasıyla Asteraceae (18), Brassicaceae ve Poaceae (13) olmuştur. Rastlama sıklığı en fazla çıkan 10 adet dar ve geniş yapraklı yabancı ot türlerinin gelişme biyolojileri BBCH skalası doğrultusunda incelenen yabancı ot türleri; Convolvulus arvensis L., Cirsium arvense (L.) Scop., Sinapis arvensis L., Galium aparine L., Cerastium sp., Chenopodium album L., Myagrum perfoliatum L., Avena fatua L., Secale cereale L., Polygonum aviculare L. olmuşlardır.