ABSTRACTThere is a strong relationship between low physical activity level and cardiovascular diseases (CVD). The popularity of football may be used to promote physical activity and previous evidence has shown it is effective to decrease the risk of CVD. However, the energy expenditure (EE) of recreational football is not well known but it is crucial to develop preventive health programmes.Fifteen sedentary middle-aged male participants were involved (mean ± SDs; age 43.9 ± 3.1 years, weight 83.0 ± 13.6 kg, height 174.9 ± 6.8 cm). EE was estimated from the heart rate (HR)-VO2 relation during 1-h 5-a-side matches (futsal). Participants covered 3412 ± 381 m in 52 ± 2 min, at an average HR of 85 ± 2% of maximum HR. Estimated EE during a recreational futsal match was 634 ± 92 kcal. One futsal recreational match corresponds to about 50% of American College of Sport Medicine recommended physical activity quantity per week. Based on this estimation: once, twice and 3 sessions per week are equivalent to 50% (634 kcal), 100% (1268 kcal) and 150% (1902 kcal), respectively, of EE suggested in international guidelines. This EE estimation may have important implications for designing recreational football training protocols in health programmes and dose response studies.