The transportation sector is responsible for about 16% of worldwide greenhouse gas emissions. Despite efforts for a sensible reduction by means of new technologies’ development, the average age of a vehicle fleet is 12.3 years in the European Union. In light of this, actions aiming at improving the efficiency of circulating vehicles can prove effective in the short to mid-term. Introducing performance packages in standard fuels could allow a reduction in the CO2 emissions of whole vehicle fleets without any modification to powertrain. Such a kind of additive is generally used in premium fuels; deposit control additives can reduce or control the deposits at intake valves and at nozzle holes with benefits for the fuel efficiency and exhaust emissions. Further improvements in combustion phasing can be achieved with cetane/octane improver. This paper aims to assess the influence of two performance packages on the exhaust emissions and fuel consumption of five vehicles set to be as representative as possible of circulating Italian passenger cars and light commercial fleet vehicles (LCVs). Based on the literature datasets, three Euro 4 vehicles were selected with a mileage representative of each single vehicle class: two passenger cars (one spark ignition and one diesel) and an LCV. Further, two diesel Euro 6 vehicles, a passenger car and an LCV, were tested to investigate the effect of fuel additives on the combustion of vehicles compliant with current homologation regulation. Exhaust emissions and fuel consumption were experimentally estimated on a chassis dynamometer over a worldwide harmonized light vehicles test cycle (WLTC) in a climate-controlled laboratory. Each vehicle was preliminarily tested when running with base fuel, then a 3000 km clean-up stage was performed using the additive package. Finally, WLTC tests were repeated. Results demonstrated the efficiency of the performance packages with a reduction between 1.2% (diesel Euro 6 passenger car) and 8.1% (diesel Euro 4 passenger car) in fuel consumption. Similar trends were found for CO2 emissions. Further, a sensible reduction in THCs, CO and PM was found for each vehicle class.