Fruit Essential Oil Research Articles

Effects of Limonene on the PAHs Mutagenicity Risk in Roasted Fish Skin

Traditional edible barbecue products use with lemon juice not only makes the barbecue more delicious but also reduces the risk of PAHs in the barbecue products. One of the major economics crops in Taiwan, the waste from citrus fruits was very tremendous mass. However, the peelings of citrus fruits are rich in essential oil, especially, the limonene is the major. Whether the anti-carcinogenesis activities of terpene, such as limonene, in citrus fruits essential oil extraction. This study to demonstrate the PAHs content in fish skin increased markedly after being roasted at 210°C for 20 minutes and greater Mutagenicity Risk of roasted fish skin was observed by Ame's test. The reduction of Mutagenicity Risk of roasted fish skin, which the anti-mutagenic abilities of substances in descending order were limonene>cold pressure oil>lemon>grapefruit. The anti-mutagenicity rate and ability of the three extracts were limonene: 18%-23%; cold-pressed lemon oil: 18%-22%; and steam distilled lemon essential oil: 8%-16%. The obvious anti- mutagenicity effects against the PAHs mutagenicity of roasted fish skins can be found in citrus fruits essential oil extraction.

Antimicrobial and Antioxidant Activities of Iranian Sumac (Rhus coriaria L.) Fruit Ethanolic Extract

Rhus coriaria L. otherwise known as sumac R coriaria, extracts are important in drug development with numerous pharmacological reputations in the South- Eastern Anatolia, Mediterranean area and Western Asia, especially in Iran. This study aimed at assaying the antimicrobial and antioxidant activities of the Iranian sumac (Rhus coriaria L.) fruit ethanolic extract. The antimicrobial activity of R. coriaria L. fruit ethanolic extract was tested against bacterial strains, including gram positive and gram negative bacteria, derived from foodstuff by micro dilution method. Furthermore, the antioxidant activity of the ethanolic extract was investigated, including scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Sumac fruit essential oil was also investigated to determine the chemical compositions by the gas chromatography (GC/MS) method. The extract showed a strong antimicrobial activity with concentration dependence and a broad antimicrobial spectrum for all tested bacteria species. Staphylococcus aureus and Salmonella enteric were found to be the most sensitive Gram positive and Gram negative bacteria respectively, with a minimum inhibitory concentration (MIC) of <0.78%. Sumac ethanolic extract showed a high antioxidant effect. The antioxidant property of sumac ethanolic extract was higher than BHT in all of the examined concentrations. Eleven constituents in the fruit’s essential oil were identified. The predominant compounds in the essential oil were malate (39.7%), Butanedioic acid, and diethyl ester (22.01%). Our findings suggest the possibility of using the fruit of R. coriaria L. as a novel source of natural antimicrobial and antioxidant agents for the food and pharmaceutical industries.

Antiadhesion activity of juniper (Juniperus communis L.) preparations against Campylobacter jejuni evaluated with PCR-based methods.

The food-borne pathogen Campylobacter jejuni can cause bacterial gastrointestinal infections. Biofilm formation amplifies the risk of human infection by improving survival and persistence of C.jejuni in food processing environments and its transmission through the food chain. We aimed to control C.jejuni using an alternative strategy of low doses of Juniperus communis fruit preparations to target bacterial adhesion properties in the first step of biofilm formation. First, we defined the anti-Campylobacter activity of a juniper fruit crude extract and its fractionated biflavonoids, flavone glycosides, and purified amentoflavone, of juniper fruit essential oil and of juniper fruit postdistillation waste material extract. For accurate quantification of adherent C.jejuni, we optimised digital Polymerase Chain Reaction (PCR) and quantitative real-time PCR for construction of standard curves and quantification. We show for the first time that juniper fruit formulations can effectively inhibit adhesion of C.jejuni to polystyrene. Furthermore, ≥94% of the antiadhesion activity of juniper fruit crude extract and juniper fruit essential oil remained under food-related conditions: modified culture medium with glucose, or a stainless steel surface, or mixed co-cultures of C.jejuni and Listeria monocytogenes. This study indicates that addition of juniper fruit formulations can control growth and adhesion of C.jejuni and thus limit food chain transmission of campylobacters.

Composition, in vitro Antibacterial and Anti-mildew Fungal Activities of Essential Oils from Twig and Fruit Parts of Eucalyptus citriodora

In this study, antibacterial and anti-mildew fungal activities of the twig and fruit essential oil and its constituents from Eucalyptus citriodora were investigated in vitro against eight bacteria and seven mildew fungi, respectively. The major ingredient responsible for the antibacterial and anti-mildew fungal activities were also identified. The essential oil from the fresh twigs and fruits of E. citriodora were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC–FID and GC–MS, respectively. The twig oil consisted primarily of 1,8-cineole (17.7%), p-cymene (17.1%), caryophyllene oxide (13.8%), and isopulegol (12.2%); the fruit oil's main constituent was p-cymene (23.2%), α-pinene (16.1%), α-cadinol (11.5%), and τ-cadinol (8.4%). In addition, comparing the antibacterial and anti-mildew fungal activities of the oils showed that the fruit oil was the most effective. For the antibacterial and anti-mildew fungal activities of the fruit oil, the active source compounds were determined to be τ-cadinol and τ-cadinol.

GC-MS ANALYSIS OF THE ESSENTIAL OIL FROM ARTEMISIA aucheri BOISS. FRUITS

The essential oil content in the fruits of Artemisia aucheri growing wild in south of Iran was found to be 2.4% (v/w). The essential oil was analyzed by gas chromatography (GC) and GC–mass spectrometry (GC–MS). Characterization of individual components was performed using a commercial mass spectrometry library, and twenty nine components, representing 89.0% of the fruits essential oil were identified. This analysis showed the presence of three volatile components, including camphor (46.5%) and 1,8-cineol (23.4%) as the main compounds.

β-Selinene-Rich Essential Oils from the Parts of Callicarpa macrophylla and Their Antioxidant and Pharmacological Activities

Background: Callicarpa macrophylla (Varbenaceae) is a medicinal shrub and is traditionally used in India, China, and South Asia. Methods: The plant material was collected from lower Himalayan region of Uttarakhand in India. The essential oils from three different aerial parts were analyzed by GC-MS. Antioxidant activity, phenolic assay, and various pharmacological activities were determined by using existing methods which are generally practiced widely. Results: Over 51, 53, and 40 compounds were identified in C. macrophylla leaves essential oil (CMLEO), C. macrophylla pre mature seeds and fruits essential oil (CMEO-I) and C.macrophylla mature seeds and fruits essential oil (CMEO-II), respectively. These oils differ in relative contents of major compounds viz; β-selinene (37.51% in CMLEO, 44.66% in CMEO-I and 57.01% in CMEO-II), phyllocladene (9.76% in CMLEO, 5.80% in CMEO-I and 12.38% in CMEO-II), caryophelline oxide (7.34% in CMLEO, 8.74% in CMEO-I and 5.0% in CMEO-II), 9E-epi-caryophelline (6.23% in CMLEO, 1.27% in CMEO-I and 3.43% in CMEO-II), longipinocarvone (4.96% in CMLEO, 1.17% in CMEO-I and 2.0% in CMEO-II), and 1,8-cineole (2.23% in CMLEO, 3.10% in CMEO-I and 1.62% in CMEO-II). The oils exhibited good in vitro antioxidant activity. The maximum activity was found in CMEO-II with IC50 values 7.37 ± 0.11, 11.49 ± 0.87, 14.59 ± 0.18, 15.66 ± 0.03, and 17.49 ± 0.13 µL/mL. The essential oils showed qualitative and quantitative diversity in the makeup of essential oils constituents. The oils were found to exhibit anti-inflammatory, analgesic, and antipyretic activity on swiss albino mice compared to the standard drugs, viz; ibuprofen, paracetamol and indomethacin. Conclusion: It is inferred from the study that the plant parts can be used scientifically in traditional systems as folk herbal medicine. Furthermore, we have generated a database for future reference and judicious exploitation of these oils from their natural setting.

Chemical composition and biological activities of leaf and fruit essential oils from Eucalyptus camaldulensis.

The chemical composition of the essential oils from the leaves and fruit of Eucalyptus camaldulensis grown in Mersin, Turkey was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. The biological activities (antibacterial and antifungal) were examined using the agar well diffusion method. The main leaf oil constituents were p-cymene (42.1%), eucalyptol (1,8-cineole) (14.1%), α-pinene (12.7%) and α-terpinol (10.7%). The main constituents of the fruit oil were eucalyptol (1,8-cineole) (34.5%), p-cymene (30.0%), α-terpinol (15.1%) and α-pinene (9.0%). Our results showed that both types of oils are rich in terms of monoterpene hydrocarbons and oxygenated monoterpenes. The leaf and fruit essential oils of E. camaldulensis significantly inhibited the growth of Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Streptococcus sp.) bacteria (p<0.05). The oils also showed fungicidal activity against Candida tropicalis and C. globrata. Leaf essential oils showed more activity than fruit essential oils, probably due to the higher p-cymene concentration in leaves.

Edible wild plant Heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils.

Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC-MS. Monoterpenes prevailed in the root oil, with β-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50=6.49-14.56μg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50>200.00μg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.

ESSENTIAL OIL AND CHEMICAL CONESTITUENTS OF FENNEL PLANTS AS AFFECTED BY COMPOST/NPK AND BIOFERTILIZATION TREATMENTS

A field experiment was conducted during the two successive seasons of 2012/2013 and 2013/2014 to explore the effect of compost/NPK and biofertilization treatments on essential oil, photosynthetic pigments and herb NPK content of fennel plants. Obtained results revealed that the best three essential oil characteristics (essential oil %, essential oil yield per plant and per feddan), photosynthetic pigments (chlorophylls a, b and carotenoids) and herb content of N, P and K values were obtained due to the use of the full dose of mineral NPK, ¼ compost + ¾ NPK dose or ½ compost + ½ NPK dose with no significant differences being detected between such three fertilization treatments. Concerning biofertilization treatments, all of the prementioned essential oil determinations, photosynthetic pigments and herb NPK content traits were considerably augmented due to the dual treatment (Minia Azotein + phosphorein) followed by Minia Azotein, while phosphorein gave the least values. In regard to the interaction gave between the, two involved factors, the highest volatile oil determinations and chemical compositions values were given by fertilizing fennel plants with the full dose of mineral NPK, 25% compost + 75% NPK or 50% compost + 50% NPK in combination with dual biofertilizer treatments (Minia Azotein + phosphorein). It is recommended, to supply fennel plants with 50% compost + 50% NPK in combination with the dual biofertilization treatment (Minia Azotein + phosphorein), from the environmental and economical point of view, in order to maximize the fruit essential oil productivity.

Antioxidant activity, polyphenolic contents and essential oil composition of Pimpinella anisum L. as affected by zinc fertilizer.

The antioxidant activity and essential oil content of plants may vary considerably with respect to environmental conditions, especially nutrient availability. Among micronutrients, zinc (Zn) is needed by plants in only small amounts but is crucial to plant development. This study aimed to evaluate the effects of Zn fertilization on the antioxidant activity, polyphenolic contents and essential oil composition of Pimpinella anisum fruit. Foliar application of Zn fertilizer considerably increased the number of detected essential oil components from 27 to 45. Zinc application at a rate of 0.2% (w/v) significantly enhanced the levels of β-bisabolene, germacrene D, n-decane and α-zingiberene, whereas the opposite trend was observed for (E)-anethole and geijerene. Application of 0.2% Zn considerably increased the levels of phenolic compounds, with chlorogenic acid showing the highest content among eight phenolic compounds detected in treated plants. The maximum antioxidant activity was achieved through application of 0.2% Zn fertilizer. These findings indicated that the quality and quantity of anise fruit essential oil components were significantly altered by application of low levels of Zn. After foliar application of Zn, polyphenolic contents as well as antioxidant activity of anise fruit increased. Using Zn fertilizer is an efficient method to improve the pharmaceutical and food properties of anise fruit. © 2017 Society of Chemical Industry.

Chemical composition and biological activities of the essential oils from different parts of Ferulago trifida Boiss

As a result of GC and GC-MS analysis of the essential oils from different parts of Ferulago trifida Boiss., a total of thirty-three compounds were identified in the flowers (Fl), stems (S), leaves (L) and fruits (Fr) essential oils, of which (E)-β-ocimene (Fl: 37.3, S: 20.7, L: 25.7, Fr: 30.5%), α-pinene (Fl: 16.3, S: 22.6, L: 19.6, Fr: 18.0%) and bornyl acetate (Fl: 9.4, S: 8.5, L: 16.7, Fr: 11.0%) were main compounds. Among the thrity-eight compounds identified in the roots essential oil, suberosin (20.7%), β-barbatene (6.6%) and cuparene (6.1%) were main compounds. All essential oils exhibited weak DPPH free radical scavenging activity (IC50: 95–120 µg ml−1). Among the tested samples flowers, fruits and roots essential oils by 72.1, 78.7 and 74.3% inhibition of Ach Enzyme, respectively, exhibited considerable AChE inhibitory effects in Elman method. They had moderate cytotoxic activity (IC50: 15–55 µg ml−1) on cancerous cell lines (MCF-7, A-549 and HT-29) in MTT assay.

Chemical Composition and Behavioral Effects of Five Plant Essential Oils on the Green Pea Aphid Acyrthosiphon pisum (Harris) (Homoptera: Aphididae).

Essential oils (EOs) from Schinus molle, Helichrysum gymnocephalum, Cedrelopsis grevei and Melaleuca viridiflora, four aromatic and medicinal plants, are commonly used in folk medicine. EOs were characterized by gas chromatography/mass spectrometry (GC/MS) and quantified by gas chromatography-flame ionization detection (GC-FID); then evaluated for their behavioral effects on adults of the green pea aphid Acyrthosiphon pisum (Harris) using a Perspex four-armed olfactometer in order to test the compatibility of their use as phytoinsecticides to control this insect pest. Our results showed that the EOs from the leaves of S.molle, M.viridiflora and C.grevei did not change aphids' behavior. However, S.molle fruits EO seemed to be attractive while H.gymnocephalum leaves EO exhibited repellency towards aphids at a dose of 10μl. The major compounds in S.molle fruits EO were 6-epi-shyobunol (16.22%) and d-limonene (15.35%). While, in H.gymnocephalum leaves EO, 1,8-cineole was the main compound (47.4%). The difference in aphids' responses to these two EOs could be attributed to the differences in their compositions. Our findings suggest that these two EOs have potential applications for the integrated pest management of A.pisum (Harris).

Antibacterial and Antioxidant Activities of the Volatile Composition of the Flower and Fruit of Solanum sisymbriifolium (Litchi Tomato)

Background: Solanum sisymbriifolium Lam. is used as traditional remedy in South America, recently this plant considered as new edible source. Berries and flower of S. sisymbriifolium have a characteristic fragrance. The pleasant fragrance of the S. sisymbriifolium could be considered as a source of food additive or preservative. Methods: The essential oils of the flower and fruit of S. sisymbriifolium Lam. (litchi tomato) were isolated by hydrodistillation method and tested for antibacterial and antioxidant potentials also these volatile oils analyzed by the gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID).The antimicrobial activity of the essential oils of fruits and flowers were tested against Staphylococcus aureus using the well diffusion method and their free-radical-scavenging activity were assessed by the 2, 2-diphenyl-picryl-hydrazyl (DPPH) assay. Results: The essential oil of flower was characterized by a high content of aldehydes and aliphatic hydrocarbons (66.8%) and the essential oil of the fruit has high amount of fatty acids and their derivatives (80.1%). Heptadecane (37.9 %) and 9,12,15-octadecatrienal (22.7%) were the main compounds in flower whereas the fruits essential oil contained hexadecanoic acid (77.4%) and ambrettolide (7.4%). The essential oils showed antibacterial activity against S. aureus in 60 and 80 µg/mL for fruit and flower, respectively. In antioxidant activity assay fruit essential oil (with100 µg/mL) showed better activity in compression to flower essential oil with 83.33% activity. Conclusion: This study showed that litchi tomato can be considered as a new source of edible compounds. Flower showed suitable antioxidant and antibacterial activity. This study also can be present an overview about chemical marker compounds of Solanum genus.

Chemical Composition, Antimicrobial and Antioxidant Properties of Schinus molle L. Essential Oil from Turkey

In traditional medicine, Schinus molle was used in treating a variety of wounds and infections due to its antibacterial and antiseptic properties. Based on this, the chemical constituents, antimicrobial and antioxidant activities of Schinus molle fruit essential oils were investigated. The hydro-distillation was used for the essential oil extraction of Schinus molle fruits and the chemical composition of extracted essential oils were identified by GC-MS. Antibacterial activities were evaluated by the agar disc diffusion method. Antioxidant properties were determined according to the TEAC method. The major components were identified as α-phellandrene (31.74%), limonene (16.82%), β-phellandrene (16.49%) and p-cymene (11.36%), respectively. The results showed that essential oils of the fruits had weak biological effects against five of eight tested bacterial and yeast species. The essential oil extract prepared from S. molle fruits had weak antioxidant activity with 4.7±1.2 mM Trolox values which monitored slightly good radical scavenging activities.

Experimental Paper. The effect of different development stages on the quantity and quality of the essential oil of Citrus aurantifolia (Christm.) Swingle in Iran

Summary Introduction: The Mexican lime tree with the scientific name of Citrus aurantifolia (Christm.) Swingle have great economic value because of its essential oil with a unique flavour. Objective: The essential oils from the peel of C. aurantifolia were collected during three development periods. Methods: The essential oil was analyzed by capillary GC and GC-MS. Results: The essential oil yields (v/w%) were 1.54%, 0.88% and 1.23%, respectively. The highest oil yield was obtained at stage I (1.54% v/w). The analysis of the essential oil indicated that limonene, β-pinene, geranial, neral and γ-terpinene were the main compounds of all samples. At the first stage, the highest percentages belonged to limonene (39.38%), geranial (14.32%) and neral (11.01%). On the other hand, the highest percentages of β-pinene and γ-terpinene (24.25% and 8.92%, respectively) were found at the final stage. Conclusion: Therefore, it is concluded that the harvest time has a considerable effect on the content and amount of lime fruit essential oil.

Molluscicidal activity of four Apiaceae essential oils against the freshwater snail Radix peregra

Snails of the family Lymnaeidae are an essential link in the transmission of zoonotic diseases. Radix peregra is a European freshwater snail and a susceptible intermediate host of Fasciola hepatica, the causing agent of fascioliasis. Essential oils (EOs) extracted from Anethum graveolens (dill), Cuminum cyminum (cumin), Foeniculum vulgare var. vulgare (bitter fennel) and Petroselinum crispum (plain leaf parsley) were characterized by GC and GC–MS. Seven EOs and 11 constituents were first screened through a single-dose bioassay against R. peregra (10 mg L−1 for juveniles and 50 mg L−1 for egg masses and mature snails). EOs from parsley, cumin and bitter fennel (leaves plus stems) were highly active towards eggs and adults at 50 mg L−1. Subsequently, dose and time–lethality bioassays were performed against adults to determine lethal parameters (LC50;90 and LT50;90). Estimated 48 h LC50s varied from 13.7 to 46.5 mg L−1, with P. crispum fruits EO exhibiting the most significant activity. EOs from cumin fruits and bitter fennel infrutescences, and cuminaldehyde, were the most time-effective treatments when assessed by continuous exposure (LT50 for a 50 mg L−1 dose = 15.1, 19.3 and 19.5 h, respectively). A short-time exposure (8 h) to bitter fennel EOs was effective for the control of adults (LT50 ≤25 h). The present study uncovers the potential of four well-known Apiaceae species as natural sources of biomolluscicides.

Composition, in vitro Cytotoxicity, Anti-mildew and Anti-wood-decay Fungal Activities of the Fruit Essential Oil of Liquidambar formosana from Taiwan

This study investigated the chemical composition, in vitro cytotoxicity, anti-mildew, and anti-wood-decay fungal activities of the essential oil isolated from the fruit of Liquidainbar formosana from Taiwan. The essential oil from the fresh fruit was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 45 compounds were identified, representing 98.5% of the essential oil. The main components identified were a-pinene (16.8%), β-caryophyllene (10.1%), τ-muurolol (8.3%), τ-cadinol (7.6%), β-pinene (6.7%), and sabinene (5.7%). The essential oil exhibited cytotoxic activity against human oral, liver, and lung cancer cells. The active source compounds were β-caryophyllene, τ-cadinol, and τ-muurolol. The fruit essential oil was shown to have excellent anti-mildew and anti-wood-decay fungal activities, the active compounds being evaluated as τ-cadinol and τ-muurolol.

Influence of Different Maturity Stages on Fruit Yield and Essential Oil Content of Some Apiaceae Family Plants B: Fennel (Foeniculum vulgare Mill.)

Influence of Different Maturity Stages on Fruit Yield and Essential Oil Content of Some Apiaceae Family Plants B: Fennel (Foeniculum vulgare Mill.)

Influence of Different Maturity Stages on Fruit Yield and Essential Oil Content of Some Apiaceae Family Plants A: Anise (Pimpinella anisum, L.)

This study investigated the effects of harvesting at different plant maturation stages on the fruit and essential oil yield as well as its compositions of anise. It was carried out at El- Baramoon Research Farm, Mansoura Horticulture Research Station, HRI, ARC, Egypt, during the winter seasons of 2013/2014 and 2014/2015. The plants were harvested at eight different fruit development stages and determinations were made of the yields of fruit , germination percentage the thousand fruit weight (seed index), the essential oil percentage and its constituents. The obtained results indicated that high yield and quality of anise fruits were obtained when fruits harvested at stage 4 (the primary umbel was matured completely).The highest essential oil percentage was higher in stage 1 (the primary umbel was at the beginning of waxy stage.). However, this high percentage of essential oil did not produce the maximum oil yield per plant in stage1 was associated with low fruit yield. Essential oil yield per plant with high Trans-Anethole content reached their maximum when fruits harvested at stage 4. Generally, it could be recommended that to obtain the maximum fruit yield and quality as well as high essential oil yield with high Trans-Anethole content, anise fruits should be harvested at stage 4 (The primary umbel was matured completely).

A Method for LC-MS/MS Profiling of Coumarins in Zanthoxylum zanthoxyloides (Lam.) B. Zepernich and Timler Extracts and Essential Oils

The metabolites from the coumarin class, present in tissues of plants belonging mainly to the Rutaceae and Apiaceae families, included compounds with high chemical diversity such as simple coumarins and furocoumarins. These health-promoting components are recognized for their valuable biological activities in herbal preparations but also for their phototoxic effects. In this work, a targeted liquid chromatography (LC) coupled with tandem mass spectrometry (MS2) was developed for the screening of 39 reference standards of coumarins and furocoumarins in essential oils and plant extracts. Chromatographic separation was accomplished on reversed phase column using water/acetonitrile as the mobile phase and detection was performed on a hybrid QqQ/linear ion trap spectrometer fitted with an atmospheric pressure chemical ionization (APCI) source operating in positive ion mode. This analytical approach was applied to investigate the coumarin compositions of fruit essential oils and methanolic extracts obtained from separated parts (fruit, leaf, stem, trunk, and root) of Zanthoxylum zanthoxyloides. Ten coumarins and six furanocoumarins were reported in this species and data analyses were used to assess the suitability of these compounds to the metabolomics-based differentiation of plant organs. The quantification criteria of the metabolites in extract samples included linearity, limit of quantification, limit of detection, and matrix effect were validated. As reported for other species of the Rutaceae family, the concentration of coumarins was drastically higher in Z. zanthoxyloides fruits than in other plant organs.