In this study we evaluated the concentration of total soluble sugars, chlorophylls a, b and total during blooming stage and fructification stage, and free amino acids and soluble proteins during fructification stage, in strawberry leaves cv. Festival in response to the addition of P in the form of phosphite in different proportions (0, 20, 30, 40 and 50 %) in the nutrient solution. When 20 % of total P in the form of phosphite was added in the nutrient solution, the concentrations of sugars significantly increased only during the blooming stage. Content of chlorophyll a during blooming stage did not show significant differences among treatments containing phosphite with respect to the control treatment; on the contrary, during the fructification stage, the highest content of chlorophyll a, b, and total was recorded with the addition of 30 % of P as phosphite in the nutrient solution. Total free amino acids had a concentration positively related to the proportion of total P as phosphite in the nutrient solution in the range of 0 to 30 %; higher percentages of phosphite led to a decrease in free amino acids. A similar trend in the concentration of total soluble proteins was observed; variable in which the lowest mean in the control was registered; being significantly different to the treatment involving the addition of 50 % of P in the form of phosphite. These results led to the conclusion that the addition of 30 % of total P to the nutrient solution as phosphite stimulates the accumulation of biomolecules in strawberry plants during the fructification stage.