Frequency Of Electric Field Research Articles

Electrically Manipulated Vapor Condensation on the Dimpled Surface: Insights from Molecular Dynamics Simulations.

Random vapor nucleation leads to flooding condensation with degraded heat-transfer efficiency. Since an external electric field has a significant effect on manipulating droplets' motion, it is possible to be one of the effective methods to hinder flooding phenomena and improve the heat-transfer rate by applying the external electric field during condensation. However, the motion of nanodroplets is more sensitive to the electric field owing to the scale effect on the nanoscale. The effect of the electric field on growth has not explicitly been comprehended. This work studied the condensation processes on a nanodimpled surface under an electric field with various strengths and directions. The results showed that condensed droplets' growth under the electric field depends on the competition between the electric field force and solid-liquid interactions. Increased vertical electric field strength, the higher torsion by the electric field hindered the motion of vapor, decreased the collision frequency for water molecules with the cooled surface, and elongated the cluster when the electric field force dominates, thus deteriorating the condensation performance. While applying the horizontal electric field, the greater electric field strength leads to better condensation performance by the larger contacting area for heat exchange. A wetting transition induced by the electric field was observed when the electric field strength increased to a certain extent (E > 5.2 × 108 V/m in this study). When the V-shaped surface replaced the dimpled surface as the condensed substrate, the same wetting transition phenomena occurred under a more significant horizontal electric field strength, showing that this method is universal. Besides, different electric field frequencies influenced both the growth and the nucleation, thus exhibiting various condensation performances.

Magnetizable Membranes Based on Cotton Microfibers, Honey, Carbonyl Iron, and Silver Nanoparticles: Effects of Static Magnetic Fields and Medium-Frequency Electric Fields on Electrical Properties

In this work, we present the manufacturing process of magnetizable membranes based on cotton microfibers, honey, carbonyl iron, and three different concentrations of silver microparticles. Each membrane is used as a dielectric material for the fabrication of electrical devices. By using the plane capacitor method, the electrical capacitance and dielectric loss tangent are measured in a medium-frequency alternating field superimposed on a static magnetic field. From the obtained data, the time constants of the devices, the components of complex dielectric permittivity, and the electrical conductivity of the membranes as a function of the electric field frequency and magnetic flux density can be extracted. The results show that the obtained membranes can be useful for the fabrication of low-cost and environmentally friendly magneto-active membranes that are required for various technical and biomedical applications.

Scattering of Metal Colloids by a Circular Post under Electric Fields.

We consider the scattering of metal colloids in aqueous solutions by an insulating circular post under the action of an AC electric field. We analyze the effects on the particle of several forces of electrical origin: the repulsion between the induced dipole of the particle and its image dipole in the post, the hydrodynamic interaction with the post due to the induced-charge electroosmotic (ICEO) flow around the particle, and the dielectrophoresis arising from the distortion of the applied electric field around the post. The relative influence of these forces is discussed as a function of frequency of the AC field, particle size and distance to the post. We perform numerical simulations of the scattering of the metal colloid by the insulating circular post flowing in a microchannel and subjected to alternating current electric fields. Our simulation results show that the maximum particle deviation is found for an applied electric field parallel to the flow direction. The deviation is also greater at low electric field frequencies, corresponding to the regime in which the ICEO's interaction with the post is predominant over other mechanisms.

Slick Synthetic Approach to Various Fluoroalkyl Silsesquioxanes-Assessment of their Dielectric Properties.

We present a smart and efficient methodology for the synthesis of a variety of fluorinated silsesquioxanes (SQs) with diverse Si-O-Si core architecture. The protocol is based on an easy-to-handle and selective hydrosilylation reaction. An investigation on the placement of the reactive Si-HC=CH2 vs. Si-H in the silsesquioxane, as well as silane vs. olefin structure, respectively, on the progress and selectivity of the hydrosilylation process, was studied. Two alternative synthetic pathways for obtaining a variety of fluorine-functionalized silsesquioxanes were developed. As a result, a series of mono- and octa- T8 SQs, tri- 'open-cage' T7 SQs, in addition to di- and tetrafunctionalized double-decker silsesquioxane (DDSQ) derivatives, were obtained selectively with high yields. All products were characterized by spectroscopic (NMR, FTIR) techniques. Selected samples were subjected to the measurements revealing their dielectric permittivity in a wide range of temperatures (from -100 °C to 100 °C) and electric field frequencies (100-106 Hz).

A Magnetically and Electrically Powered Hybrid Micromotor in Conductive Solutions: Synergistic Propulsion Effects and Label-Free Cargo Transport and Sensing.

Electrically powered micro- and nanomotors are promising tools for in vitro single-cell analysis. In particular, single cells can be trapped, transported, and electroporated by a Janus particle (JP) using an externally applied electric field. However, while dielectrophoretic (DEP)-based cargo manipulation can be achieved at high-solution conductivity, electrical propulsion of these micromotors becomes ineffective at solution conductivities exceeding ≈0.3 mS cm-1 . Here, JP cargo manipulation and transport capabilities to conductive near-physiological (<6 mS cm-1 ) solutions are extended successfully by combining magnetic field-based micromotor propulsion and navigation with DEP-based manipulation of various synthetic and biological cargos. Combination of a rotating magnetic field and electric field results in enhanced micromotor mobility and steering control through tuning of the electric field frequency. In addition, the micromotor's ability of identifying apoptotic cell among viable and necrotic cells based on their dielectrophoretic difference is demonstrated, thus, enabling to analyze the apoptotic status in the single-cell samples for drug discovery, cell therapeutics, and immunotherapy. The ability to trap and transport live cells towards regions containing doxorubicin-loaded liposomes is also demonstrated. This hybrid micromotor approach for label-free trapping, transporting, and sensing of selected cells within conductive solutions opens new opportunities in drug delivery and single-cell analysis, where close-to-physiological media conditions are necessary.

Ionic Chiral Ferrocene Doped Cholesteric Liquid Crystal with Electronically Tunable Reflective Bandwidth performance.

Cholesteric liquid crystals (CLC) were widely used in optical devices as one-dimensional photonic crystals. However, their reflective bands cannot be adjusted, which limits their wide application in many fields. In this paper, a series of ionic chiral ferrocene derivatives (CD-Fc+) as dopants were designed and prepared, and their doping into negative liquid crystal matrix was investigated to develop cholesteric response liquid crystal composites with electrically tunable reflective bands. The effects of electric field frequency, voltage, retention time of voltage and molecular structure on the broadening of reflection bandwidth were investigated in detail.

Finite-Element Simulation of Electroosmotic Mixing: A Study of the Simultaneous Effects of Working Parameters for Optimization

Micromixers are crucial parts of microfluidic systems when it comes to efficiency and precision, as mixing is the central process in most relevant applications, including medical diagnosis, chemical production, and drug discovery. In view of the importance of improving the mixing quality, for the first time, the present work investigates the simultaneous effects of mixing chamber geometry (circular, hexagonal, and octagonal), electric field frequency (5, 7, 10, and 15 Hz), inlet velocity (0.1-0.2 mm·s−1), and phase difference (0-π) on the flow inside an electroosmotic micromixer using the finite-element tool COMSOL Multiphysics 5.4 to optimize the process and achieve homogeneous mixing. The flow-field, concentration-field, and electric-field equations were coupled and solved simultaneously. The results of this research indicated that at a given inlet velocity and a specific frequency range, as frequency increases, more mixing occurs in a smaller chamber, and as the inlet velocity increases, more mixing occurs in a smaller chamber at a higher frequency. Moreover, the highest mixing level (98.16%) was obtained with a 0.1 mm·s−1 inlet velocity, 10 Hz frequency, and π/2 phase difference in a hexagonal chamber.

Study on the Performance of an Electric-Field-Enhanced Oil–Water Separator in Treating Heavy Oil with High Water Cut

As most offshore oilfields come to the middle- and late-exploitation period, and with the popularization of tertiary enhanced oil recovery (EOR) technology, the physical properties of produced fluids become more complex, bringing new challenges to oil-water separation. Conventional solutions, such as increasing the input of chemicals, enlarging the volume of the separator equipment, and extending the processing, are usually uneconomical and space-wasting. Electric-field-enhanced oil-water separation equipment was developed in this paper, and a mine field test was carried out. With the average water cut of different sampling ports and the overall dehydration rate taken as indicators, the effects of electric field frequency, inlet flow, chemical type, and addition upon the separation of the device were evaluated. The experimental results showed that for the ABJ mixed liquid, the optimal operating frequency of the electric field is above 3500 Hz. Compared with traditional separation equipment, the advantages of the electric-field-enhanced oil–water separation equipment are more significant in large flow conditions. When the water cut of the platform inlet fluctuates between 78% and 97%, and without the addition of chemicals, the average water cut was reduced to 7% and the average dehydration rate reached 90%, an improvement of about 15%.

Magnetoimpedance modulation in a planar magnetoelectric ferromagnet – piezoelectric heterostructure

The effect of a giant change in the impedance of ferromagnetic materials under the action of an external magnetic field is widely used to elaborate highly sensitive magnetic field sensors. The purpose of this work was to demonstrate the possibilities of controlling the magnitude of the magnetoimpedance in a ferromagnet-piezoelectric structure using an electric field. Method. In the measurements, we used a planar heterostructure containing a strip of amorphous ferromagnet Metglas, 25 µm thick and 25 mm long, mechanically connected to a bimorph, 0.5 mm thick and 30 mm long, made of piezoceramic lead zirconate titanate. An alternating current with a frequency of 30 kHz...10 MHz was passed through the strip, the structure was placed in a longitudinal permanent magnetic field of 0...500 Oe, an alternating electric field up to 400 V/cm with a frequency of 60 Hz...50 kHz was applied to the piezobimorph, and the change in the impedance of the strip was recorded. Results. In the absence of electric field, a narrowing of the magnetoimpedance magnetic fields region with a decrease in the current frequency and saturation of the magnetoimpedance in magnetic fields above 334 Oe were observed. The maximum value of the magnetoimpedance reached 18% at a current frequency of 1 MHz. The application of electric field to the piezobimorph led to the appearance of side components in the frequency spectrum of the voltage on the ferromagnetic layer, which indicates the amplitude-phase modulation of the magnetoimpedance. The amplitude modulation coefficient reached a maximum value of 6 · 10−3 for the electric field frequency of 11.2 kHz and decreased monotonically with an increase in the magnetic field. The modulation of the magnetoimpedance occurs due to the converse magnetoelectric effect in the heterostructure, which leads to the modulation of the magnetization of the ferromagnetic layer, and the subsequent change in the relative magnetic permeability and thickness of the skin layer in the ferromagnet. The results obtained can be used to create magnetic fields sensors controlled by an electric field.

Modulated Collective Motions and Condensation of Bacteria

Bacteria can spontaneously develop collective motions by aligning their motions in dense systems. Here we show that bacteria can also respond collectively to an alternating electrical field and form dynamic clusters oscillating at the same frequency of the field. As the dynamic clusters go beyond a critical size, they split into smaller ones spontaneously. The critical size for splitting depends on the frequency of electric field and the concentration of bacteria. We show that, instead of their biological activity, the physical properties of bacteria as charged particles are responsible for the formation of dynamic clusters. Electroconvective flows across the system play the key role in stabilizing the clusters. However, to form clusters, collective hydrodynamic cooperation between bacteria is important such that no aggregation occurs in dilute suspensions. The findings in this study illustrate that bio-systems can respond collectively to an external field, promising an effective way to control and modulate the behavior of organisms. Moreover, the controlled aggregation and condensation of bacteria offer a robust approach to improve the local concentration of bacteria for early and rapid detection, which has wide applications in clinics.

Controlling the condensation of vapor by electric field: A molecular dynamics simulation study

In this study, the initial stages of the condensation processes of water vapor under a series of constant electric fields and alternative electric fields parallel to the surface are carried out by molecular dynamics simulation. The results show that under a constant electric field, the rearrangement of water molecular structure induced by electric field leads to cluster deformation, and the change of diffusion rate leads to the condensation efficiency to increase first and then decrease with the increase of the applied electric field. Compared with the one without applied field, the condensation efficiency can be doubled under the applied filed of 0.8 V/nm. Further increase of the applied field to 5.0 V/nm, nearly no condensation occurs. Under an alternative electric field, water molecules are unable to respond to the field variation at increased electric field frequency, which leads to reduced cluster deformation. The condensation efficiency of water molecules decreases with the increase of alternative electric field frequency. Meanwhile, the increase of alternating electric field frequency significantly prolongs the nucleation time and increases the critical nucleus size. The results indicate that the electric field can be a new avenue to regulate the condensation of vapor towards more efficient water collection and heat transfer.

Synthesis and characterization of new multiferroic (BiBa)(FeTi2)O8

The present communication reports the study of various properties (i.e., structural, electrical, dielectric, ferroelectric, and magnetic) of new multiferroic: (BiBa)(FeTi2)O8 synthesized by a mixed oxide reaction route. The analysis of the X-ray diffraction profile at room temperature ensures its phase formation in the orthorhombic crystal structure. The micrographic study divulges irregular shape grains (mostly rectangular) with an average grain size of 3.5 μm. The compositional study displays the desired stoichiometry. The study of electrical (impedance, modulus, and conductivity) and dielectric properties (dielectric constant and tangent loss) of the sample at different temperatures (RT – 773 K) and electric field frequency (1 kHz-1MHz) provides many new experimental data, and insight mechanisms. The temperature dependence of the dielectric constant exhibits a dielectric anomaly around 523 K that may be due to the combined effect of oxygen vacancy mediated redox reaction (Fe3+→Fe2+ and Ti4+→Ti3+) and Maxwell-Wagner polarization. The loss tangent follows a similar trend to that of the dielectric constant except for an additional low-frequency relaxation peak of about 543 K. The dielectric loss tangent exhibits low value in low temperature and high-frequency regions, indicating that the sample can be a potential candidate for data storage devices. The ferroelectric recording shows a non-linear PE loop at room temperature. The room temperature magnetic recordings show evidence of weak ferromagnetism due to suppression of spiral spin structure as opposed to the antiferromagnetic nature of BiFeO3. The coexistence of these unique features enables the synthesized sample to be used in voltage-controlled data storage devices.

Microfluidic device for the Separation of non-metastatic (MCF-7) and non-tumor (MCF-10A) breast cancer cells using AC Dielectrophoresis.

Dielectrophoretic devices are capable of the detection and manipulation of cancer cells in a label-free, cost-effective, robust, and accurate manner using the principle of the polarization of the cancer cells in the sample volume by applying an external electric field. This article demonstrates how a microfluidic platform can be utilized for high-throughput continuous sorting of non-metastatic breast cancer cells (MCF-7) and non-tumor breast epithelial cells (MCF-10A) using hydrodynamic dielectrophoresis (HDEP) from the cell mixture. By generating an electric field between two electrodes placed side-by-side with a micron-sized gap between them in an HDEP microfluidic chip, non-tumor breast epithelial cells (MCF-10A) can be pushed away, exhibiting negative DEP inside the main channel, while the non-metastatic breast cancer cells follow their course unaffected when suspended in cell medium due to having conductivity higher than the membrane conductivity. To demonstrate this concept, simulations were performed for different values of medium conductivity, and the sorting of cells was studied. A parametric study was carried out, and a suitable cell mixture conductivity was found to be 0.4 S/m. By keeping the medium conductivity fixed, an adequate AC frequency of 0.8 MHz was established, giving maximum sorting efficiency, by varying the electric field frequency. Using the demonstrated method, after choosing the appropriate cell mixture suspension medium conductivity and frequency of the applied AC, maximum sorting efficiency can be achieved.

Experimental Analysis on the Dependence of the Capacitance of Magnetorheological Fluids on Frequency

The capacitance characteristics of magnetorheological fluids (MRFs) were studied experimentally based on simulation analysis. The nonlinear relationship between the capacitance of MRFs and electric field frequency was measured by the self-made circuit device. The effects of magnetic induction intensity and particle volume fraction on the capacitance characteristics of MRFs were investigated. The results show that the nonlinear dependence of the capacitance of an MRF on frequency induced by the effect of tunnel current decreases with the increase in frequency. The capacitance of an MRF is directly related to the particle structures. The capacitance of the MRF with a chain particle structure is greater than that of the MRF with a random particle distribution. The smaller the clearance between the adjacent particles, the greater the capacitance of the MRF. The network particle structure and particle contact will reduce the capacitance of an MRF. The capacitance of an MRF increases with the increment of external magnetic field. The capacitance of an MRF with higher particle volume fraction is smaller than that of an MRF with lower particle volume fraction.

Metal-ligand Lability and Ligand Mobility Enables Framework Transformation via Ligand Release in a Family of Crystalline 2D Coordination Polymers.

A family of seven silver(I)‐perfluorocarboxylate‐quinoxaline coordination polymers, [Ag4(O2CRF)4(quin)4] 1–5 (RF=(CF2)n‐1CF3)4, n=1 to 5); [Ag4(O2C(CF2)2CO2)2(quin)4] 6; [Ag4(O2CC6F5)4(quin)4] 7 (quin=quinoxaline), denoted by composition as 4 : 4 : 4 phases, was synthesised from reaction of the corresponding silver(I) perfluorocarboxylate with excess quinoxaline. Compounds 1–7 adopt a common 2D layered structure in which 1D silver‐perfluorcarboxylate chains are crosslinked by ditopic quinoxaline ligands. Solid‐state reaction upon heating, involving loss of one equivalent of quinoxaline, yielding new crystalline 4 : 4 : 3 phases [Ag4(O2C(CF2)n‐1CF3)4(quin)3]n (8–10, n=1 to 3), was followed in situ by PXRD and TGA studies. Crystal structures were confirmed by direct syntheses and structure determination. The solid‐state reaction converting 4 : 4 : 4 to 4 : 4 : 3 phase materials involves cleavage and formation of Ag−N and Ag−O bonds to enable the structural rearrangement. One of the 4 : 4 : 3 phase coordination polymers (10) shows the remarkably high dielectric constant in the low electric field frequency range.

Mass transfer intensification for carbon quantum dot nanofluid drops under pulsed electric fields

Simultaneous use of carbon quantum dot (CQD) nanofluids and pulsed electric fields exhibits amazing mass transfer intensification in liquid–liquid extraction of circulating drops. Here, the chemical system of kerosene–acetic acid–water with mass transfer resistance in the organic phase was used in which organic nanofluid drops contained CQD or modified CQD-Fe. These products with extremely small sizes of 7.2 and 13.4 nm were synthesized and characterized by DLS, Zeta potential, XRD, EDS and SEM techniques. To find optimum conditions, CQD concentrations within (0.0005–0.003) wt%, electric field frequencies within (50–550) Hz and electric field strengths to 16 V/cm were examined. From hydrodynamic point of view, the flow pattern of drops was in circulating mode, and that terminal velocity of drops correctly followed the Grace model. The substantial effect of pulsed electric field on the CQD and CQD-Fe nanofluids, brought about mass transfer enhancements to 263.5 and 291.6%. This can be attributed to the electro-induced motion of global CQDs with pulsed electric fields. For the aim of modelling, the adapted Kumar and Hartland equation with a developed correlation of the enhancement factor versus involved dimensionless variables were satisfactory to reproduce the mass transfer coefficient data.

Enormous electro-optic effect in paraelectric nanodisordered KTa1-xNbxO3 crystal.

Recent experiments have revealed that the order of the electro-optic (EO) effect depends on the frequency of electric field in paraelectric nanodisordered KTa1-xNbxO3 (KTN) crystal. Through the dielectric frequency spectrum under the bias electric field, enormous linear and quadratic EO effects were discovered at the resonance frequencies, which changed the perception that only the quadratic EO effect exists. Applying just a small AC electric field of 6 V/mm, the effective linear EO coefficient reached 478 pm/V at 609 kHz, and the effective quadratic EO coefficient reached 4.39*10-13m2V-2at 302 kHz. The reason why an extremely low electric field results in an enormous EO coefficient is attributed to the resonance between the polar nanoregions (PNRs) and the electric field, induced by the field-driven reorientation of free dipoles on the boundary of the PNRs. In addition, the order of EO effect depending on the frequency of electric field was attributed to the motion modes of the PNR. This finding improves the understanding of how the EO effect is caused by field-driven PNR dynamics, but also provides a basis for the development of EO devices.

The Application and Optimization of HIPEF Technology in the Processing of Juice from Strawberries Harvested at Two Stages of Ripeness.

The aim of this study was to investigate the influence of high intensity pulsed electric field (HIPEF) technology on the stability of total phenols, anthocyanins, hydroxycinnamic acids, flavonols, and condensed tannins in strawberry juices (Fragaria x ananassa Duch. cv. ‘Albion’) with different ripening stages (75% and 100%) and stored at +4 °C for 7 days. The HIPEF parameters studied were: (i) electric field strength (40 and 50 kV cm−1), (ii) frequency (100 and 200 Hz), and (iii) treatment duration (3 and 6 min). Of the HIPEF parameters studied, electric field strength and frequency had a statistically significant effect on the content of all phenolic compounds. Treatment duration showed no statistically significant effects on phenolic compounds except for flavonols and condensed tannins. Storage had a positive effect on the stability of most of the phenolic compounds, with the exception of flavonols. Optimization of HIPEF processing showed that strawberry samples at both ripeness levels were suitable for HIPEF treatment to obtain functional fruit juices with a high content of polyphenols.

P-015 Automated, efficient sorting of sperm using dielectrophoresis (DEP), utilizing the electrical properties of the tail as a biomarker of the viability, even for immotile cells

Abstract Study question Developing a simple and automated method and device that sorts semen cell by cell for eventual application to retrieving rare sperm from severely oligozoospermic samples. Summary answer Over 90% of the viable cells in a sample can be sorted out from non-viable sperm cells and other debris using this method. What is known already The varying electrical properties of different cell types can result in unique frequency dependent dielectrophoretic (DEP) behavior when they are exposed to alternating current (AC). They are either repelled or attracted depending on their properties and the field frequency. This has been applied to sorting rare cells such as circulating tumor cells from blood. We have previously shown that the head and tail of human sperm have different electrical properties. As a result, at certain frequencies of an AC electric field the tails of viable cells (even immotile ones) are attracted to electric field gradients while the heads are distanced. Study design, size, duration Semen samples from several patients at Rambam medical center were collected and cryopreserved. Later the thawed semen samples were then tested in our sorting device. At least one hundred live cells were evaluated in each sample. The percentage of live cells that were successfully sorted out of the original mixture of live sperm cells, dead sperm cells and other debris was assessed. Participants/materials, setting, methods After thawing the samples were stained using CFDA and PI for visual live/dead identification (in actual practice no staining is necessary). The device consists of a polydimethylsiloxane (PDMS) microchannel on a glass slide patterned with Indium Tin Oxide (ITO) transparent electrodes. In the device the sperm are transferred to a low conductivity buffer. A curved electrode then sorts them out of the stream of the cell mixture to an alternate exit. Main results and the role of chance We have successfully demonstrated a new biomarker of live sperm (even immotile ones). The tails of live sperm have a positive (attractive) DEP response while dead sperm react negatively and are repelled from field gradients. At the specific frequencies used the head is simultaneously distanced from the electric fields helping to prevent damage to the DNA during the sorting. We also showed that this effect could be used to sort sperm with over 90% efficiency from a sample with dead sperm and other debris. Since each cell passes the electrodes and is automatically sorted it could possibly be used for severely oligozoospermic and cryptozoospermic samples to find sperm and make more sperm available for more discriminatory sperm selection in such samples. Nowadays such samples are searched manually and the minute number of sperm present is often missed leading to a mistaken azoospermic diagnosis and possibly unnecessary surgery. The components are relatively low cost and the chips disposable allowing for wider implementation. Significant progress was also made in preventing sperm from sticking to the substrates using coatings and a vibrational motor. Additionally, a proof of concept parallelization of the electrodes to increase the throughput was also demonstrated. Limitations, reasons for caution The inherent difficulty with microfluidic technologies is low throughput. Further work is needed to effectively parallelize and optimize the system. Effective pre-processing to clean the sample may also help to increase the throughput. Wider implications of the findings This biomarker can potentially be useful in determining an immotile cell’s viability for ICSI. This technology can also be of potential use for testicular tissue samples where there are many other cell types and debris and it is difficult to find rare sperm. Advanced sperm selection can also be incorporated. Trial registration number not applicable

Abstract 3252: Tumor treating fields suppression of ciliogenesis enhances temozolomide toxicity

Abstract Tumor Treating Fields (TTFields) are low intensity, alternating intermediate frequency (200kHz) electrical fields that extend survival of glioblastoma patients receiving maintenance temozolomide (TMZ) chemotherapy. How TTFields exert efficacy on cancer cells over normal cells, or interact with TMZ is unclear. Primary cilia are microtubule-based organelles triggered by extracellular ligands, mechanical and electrical field stimulation, and are capable of promoting cancer growth and TMZ chemoresistance. We found in both low and high grade patient glioma cell lines that TTFields ablated cilia within 24 hours. Halting TTFields treatment led to recovered frequencies of elongated cilia. Cilia on normal primary astrocytes, neurons and multiciliated ependymal cells were less affected by TTFields. The TTFields-mediated loss of glioma cilia was partially rescued by chloroquine pretreatment, suggesting the effect is in part due to autophagy activation. We also observed death of ciliated cells during TTFields by live imaging. Notably, TMZ-induced stimulation of ciliogenesis in both adherent cells and gliomaspheres was blocked by TTFields. Moreover, the inhibitory effects of TTFields and TMZ on tumor cell recurrence correlated with the relative timing of TMZ exposure to TTFields and ARL13B positive cilia. Finally, TTFields disrupted cilia in patient tumors treated ex vivo. Our findings suggest TTFields efficacy may depend on the degree of tumor ciliogenesis and relative timing of TMZ treatment. Citation Format: Ping Shi, Jia Tian, Brittany Ulm, Julianne Mallinger, Habibeh Khoshbouei, Loic Deleyrolle, Matthew Sarkisian. Tumor treating fields suppression of ciliogenesis enhances temozolomide toxicity [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3252.