Free Radical Oxidation Research Articles

Redox control of tumor cell apoptosis during hypoxia

Currently, close attention is paid to studies aimed at searching for redox-sensitive targets for the regulation of tumor cell death. Tumor growth is characterized by impaired cell proliferation, differentiation, and apoptosis against the background of oxidative stress. Hypoxia contributes to the formation of mitochondrial dysfunction and acts as an additional factor that exacerbates oxidative stress in the tumor cell. Reactive oxygen species are general damaging factors, however, they can act as modulators of processes such as reception, intracellular signaling, proliferation, apoptosis, while taking part in the functioning of the cell redox system and contributing to the oxidative modification of macromolecules. One of the possible reasons for the activation of the production of reactive oxygen species is the low content of O2 in the cell, the final electron acceptor to ensure the functioning of the enzymes of the mitochondrial respiratory chain. The glutathione system makes a significant contribution to maintaining the balance between prooxidants and antioxidants in the cell. The role of this system is justified by the reduction potential of glutathione, which, acting as an acceptor of hydroxyl ions and singlet oxygen, significantly reduces the cytotoxic and damaging effects of reactive oxygen species. At the same time, it serves as a coenzyme for glutathione-dependent enzymes, which play a leading role not only in providing antioxidant processes, but also in maintaining the thiol disulfide balance. Hypoxia, which acts as a factor in the activation of free radical oxidation against the background of proliferation and apoptosis dysregulation, contributes to the formation of resistance of tumor cells to chemotherapeutic effects. In light of this, the importance of studying the redox-dependent mechanisms involved in the regulation and implementation of tumor cell death under insufficient oxygen supply becomes obvious, which is necessary for the development of personalized antitumor therapy. The article presents a review of modern literature, including the results of our own research, on the role of the thiol disulfide system and oxidatively modified proteins in the redox regulation of proliferation and apoptotic death of tumor cells, including under hypoxic conditions.

Peredaman Radikal Bebas Ekstrak Etanol Daun Rosella (Hibiscus sabdariffa L) Dengan Metode Frap (Ferric Reducing Antioxidant Power)

Background: Antioxidant are chemical compounds that can donate electrons to unpaired free radicals, thereby reducing the effects of free radical oxidation. One of the plants that have antioxidants activity is the rosella plant, for example in the leaves. Rosella leaves contain phytochemical compounds that are useful as antioxidant, namely flavonoids, alkaloids, saponins, tanin.Objective: Determine the antioxidant activity of the ethanol extract of rosella leave (Hibiscus sabdariffa L) by FRAP method.Method: Rosella leaf was macerated using 70% ethanol as solvent. Then filtered and thicken using a frying pan on an electric stove over medium heat of approximately 300 watt. Phytochemical screening was carried out, namely flavonoids, alkaloids, saponins, tanin and TLC test to indicate the presence of flavonoid compounds as antioxidants. Quantitative analysis was carried out to determine antioxidant activity with the FRAP method measured using a UV-Vis spectrophotometer.Result: The antioxidant activity test of the ethanol extract of rosella leaves has FRAP value of 1,749 mmol FeSO4/mg. Based on statistical analysis T-test, shows that there is a difference between the sample and the standard with a significant value (p<0,05) is 0,001.Conclusion: The antioxidant activity test on rosella leaves with the FRAP method proved that the ethanol extract of rosella leaves had antioxidant content with a FRAP value of 1,749 mmol FeSO4/mg.

Combination of HPLC–orbitrap‐MS/MS and network pharmacology to identify the anti‐inflammatory phytochemicals in the coffee leaf extracts

AbstractIn this study, we investigated the phytochemical compositions and the associated anti‐inflammatory activity of coffee leaf fractions prepared by sequential solvent extraction using high‐performance liquid chromatography–orbitrap‐tandem mass spectrometry (HPLC–orbitrap‐MS/MS) combined with network pharmacology. The results showed that the ethyl acetate fraction (EAC‐L) had the highest nitric oxide (NO), ABTS, and DPPH free radical scavenging abilities due to the higher concentrations of mangiferin, rutin, 3,5‐dicaffeoylquinic acid (3,5‐diCQA), and 4,5‐diCQA. The extraction solvents had the greatest impact on the anti‐inflammatory activity of coffee leaf fractions, whereas the processing method had the most significant effect on the antioxidant activity of these fractions. Untargeted metabolomics analysis using HPLC–orbitrap‐MS/MS indicated that palmitic acid, 3,4‐dihydroxybenzaldehyde, and caffeic acid may be involved in the anti‐inflammatory activity of EAC‐L fraction obtained from fresh coffee leaves. On the other hand, processed coffee leaf fraction exhibited anti‐inflammatory activity that was attributed to the presence of 9S,13R‐12‐oxophytodienoic acid, pinocembrin, and quercetin, which have high degree values associated with the inflammation network. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment of network pharmacology analysis showed that these 35 differential compounds in the coffee leaf fractions affect cell transcription, apoptosis, phosphorylation, NO synthesis, phosphatidylinositide 3‐kinases‐protein kinase B (PI3K‐Akt) signaling pathway, focal adhesion, hypoxia‐inducible factor‐1, hepatitis, cancer, and so on. This result indicated that coffee leaf extract may also function as an inhibitor for inflammation‐related cancers. The findings of our research are valuable in guiding the extraction of anti‐inflammatory components from coffee leaves.

Oxidative modification of proteins and antioxidant status in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes

Modern aspects of the pathogenesis of acute inflammation of the peritoneum that is concurrent with diabetes involves analysis of metabolic mechanisms, in particular peroxidaton of proteins – antioxidant defense. Therefore, the objective of our study was to examine the interrelation between the processes of free-radical oxidation of proteins and antioxidant system in the dynamics of development of acute generalized peritonitis against the background of streptozotocin-induced diabetes. The study was performed on 56 non-linear white mature male rats. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin (60 mg/kg). On the 14th day of the development of streptozotocin-induced diabetes, we injected 10% filtrated faeces suspension (0.5 mL) into the abdominal cavity, thus initiating acute generalized peritonitis. Oxidative modification of proteins in blood serum was studied using the I. F. Meshchyshen’s method and the condition of antioxidant protection was monitored according to the activities of superoxide dismutase, catalase, content of reduced glutathione, and the level of ceruloplasmin. The study of the parameters of free-radical oxidation of proteins and study of the condition of antioxidant system in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes demonstrated that oxidative protein modification grew, while the parameters of the activity of antioxidant system were being inhibited, depending on the stage of acute inflammation of the peritoneum. We determined inverse correlation relationships between the products of free-radical oxidation of proteins and parameters of antioxidant system on the third and on the seventh days of modelling of combined pathology. Manifestation of acute generalized peritonitis concurring with streptozotocin-induced diabetes was accompanied by a gradual accumulation of the products of free-radical oxidation of proteins and exhaustion of the antioxidant defense during all stages of the development of acute inflammation of the peritoneum, peaking on the seventh day after administration of faecal suspension (terminal stage of peritonitis). The observed inverse correlations between the levels of oxidative modification of proteins and the activity of superoxide dismutase, catalase, reduced glutathione, and ceruloplasmin on the third and on the seventh days of modelling of combined pathology indicate a predictive role of the processes of free-radical oxidation of proteins in exhaustion of antioxidant-defense resources.

Parameters of free radical oxidation and antioxidant defense in patients with cervical cancer before and after radical surgical treatment

Purpose of the study. To analyse free radical oxidation and antioxidant defense in patients diagnosed with early cervical cancer (CC) before and after radical surgical treatment.Patients and methods. Levels of diene conjugates, malondialdehyde (MDA), superoxide dismutase (SOD), catalase, glutathione, glutathione- dependent enzymes, vitamins A and E were determined in 74 women under the age of 45 (48 patients those who were at the stage of surgical treatment with a diagnosis of CC at the National Medical Research Center of Oncology in the period 2017–2020 and 26 healthy women).Results. Patients with early CC showed significant changes in the intensity of lipid peroxidation processes and in antioxidant defense: elevate levels of MDA and diene conjugates, initial decline in the activity of SOD and catalase, low levels of vitamins A and E. These results complete the understanding of the processes occurring in the body of an oncological patient at the initial stage of tumor formation, which does not yet have an obvious clinical manifestation. After total removal of the ovaries, most of the indicators characterizing the enzymatic link of the antioxidant system tend to normalize, while the violation of the content of vitamins E and A (related to the non-enzymatic link of the antioxidant system) worsens.Conclusions. Desynchronization of free radical oxidation processes with multidirectional changes in oxidation and antioxidation in patients with early CC at the stage of radical surgical treatment should be considered from the position of hormone‑ reducing surgery and a resulting complex of changes in the organs and systems of women with cancer.

Effect of the complex of natural terpenes and vitamin E Renotineх® on free radical processes in patients with urolithiasis

BACKGROUND: One of the leading links in the pathogenesis of urolithiasis is oxidative stress, which occurs as a result of the intensification of free radical oxidation with insufficient activity of the antioxidant defense system of the body.
 AIM: The aim of the study was to study the effect of a complex of natural terpenes and vitamin E Renotineх on the state of oxidative stress in patients with urolithiasis in the postoperative period after percutaneous nephrolithotripsy.
 MATERIALS AND METHODS: The study included 42 patients. Of the 35 people who underwent percutaneous nephrolithotripsy, 2 groups were formed. Patients of the main group (n = 20), in contrast to the control group (n = 15), received Renotinex (Polaris LLC, Russia) before and after surgery, 2 capsules (1 capsule 300 mg) twice a day. To determine the main indicators of the activity of free-radical processes in the body, all patients underwent a blood test on the 3rd day after surgery. The total activity of the pro- and antioxidant systems in blood plasma was determined by the method of induced chemiluminescence. The activity of lipid peroxidation was assessed by determining the concentration of active products of thiobarbituric acid secondary products of lipid peroxidation in blood erythrocytes. The activity of antioxidant enzymes was studied by spectrophotometric method. To determine the ability of blood plasma to crystallize in some of the same patients with urolithiasis while taking Renotinex, the method of wedge dehydration was used. For a comparative analysis of the crystal structure of dried blood plasma samples (facies), 7 healthy volunteers were included in the study, which made up the third group (comparison group).
 RESULTS: The results of the study reliably demonstrate that the composition of the complex of natural terpenes and vitamin E Renotinex has a positive effect on free-radical processes in patients with urolithiasis, biochemical parameters of blood plasma (indicators of induced chemiluminescence, catalase activity, malondialdehyde level), as well as on crystallization processes blood plasma, which is confirmed by the method of wedge dehydration of blood plasma. Any change in the body caused by excessive activity of free-radical oxidation processes is reflected in the composition of the blood, and specifically in the indicators of the mechanical characteristics of blood plasma (viscosity, structure, surface tension), which determine the shape of the resulting structures of the drying drop of biological fluid.
 CONCLUSIONS: The complex of natural terpenes and vitamin E Renotinex has a positive effect on free-radical processes, biochemical parameters of blood plasma, as well as on the systemic structural organization of blood plasma in patients operated on for urolithiasis. The inclusion of the combined product Renotinex in the treatment regimen for patients with urolithiasis is pathogenetically justified.

Efficient Photo-Oxidation Leaching of Ni and Co in a Spent Lithium-Ion Battery Cathode by Homogeneous UV/H2O2

Existing lithium-ion battery (LIB) leaching and recycling techniques have been widely applied in industrial production despite problems, such as difficult operation, high cost, and heavy pollution. Herein, we propose a novel photochemical oxidation method to leach Co and Ni ions in spent LIB cathodes, achieving leaching efficiencies of 99.73 and 97.54% when irradiated with hydrogen peroxide as the oxidant in acetonitrile and dichloromethane as the solvent under UV light for 4 h. The oxidation mechanism of the reaction was investigated by a series of tests. Three free radical oxidation pathways that functioned together with the reaction mechanism were proposed. This study is the first to combine the UV/H2O2 photo-oxidation reaction with spent LIB recycling to achieve a universal, green, and simple operation path for metal recycling field in the future.

Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases.

Introduction: Free radicals are reactive oxygen species that constantly circulate through the body and occur as a side effect of many reactions that take place in the human body. Under normal conditions, they are removed from the body by antioxidant processes. If these natural mechanisms are disrupted, radicals accumulate in excess and contribute to the development of many diseases. Methodology: Relevant recent information on oxidative stress, free radicals, reactive oxidative species, and natural and synthetic antioxidants was collected by researching electronic databases such as PubMed / Medline, Web of Science, and Science Direct. Results: According to the analysed studies, this comprehensive review provided a recent update on oxidative stress, free radicals and antioxidants and their impact on the pathophysiology of human diseases. Discussion: To counteract the condition of oxidative stress, synthetic antioxidants must be provided from external sources to supplement the antioxidant defense mechanism internally. Because of their therapeutic potential and natural origin, medicinal plants have been reported as the main source of natural antioxidants phytocompounds. Some non-enzymatic phytocompounds such as flavonoids, polyphenols, and glutathione, along with some vitamins have been reported to possess strong antioxidant activities in vivo and in vitro studies. Thus, the present review describes, in brief, the overview of oxidative stress-directed cellular damage and the unction of dietary antioxidants in the management of different diseases. The therapeutic limitations in correlating the antioxidant activity of foods to human health were also discussed.

The Effect of Whole Blood and Bone Marrow with the Addition of Pyrimidine-2,4(1h,3h)-dione Thietanyl Derivatives on Free Radical Oxidation.

It is relevant to study the general patterns and identify non-specific mechanisms of body protective and adaptive reactions violation, which can lead to the various pathological processes and develop principles for the correction of these disorders. One of the therapy and prevention directions is the search for new medicines. In recent years, new derivatives of pyrimidine bases have been synthesized and studied. Pyrimidine-based medicines have a membrane-stabilizing and immunomodulatory effect and can normalize metabolic disorders and increase the oxidative activity of leukocytes. Disruption of the free radical oxidation processes, the generation of reactive oxygen species and lipid peroxidation, including in whole blood and bone marrow, has gained importance in recent years. Each reaction was monitored by thin layer chromatography. 1H, 13C, and 15N NMR spectra were recorded (chemical shifts were expressed as δ-values). We studied the effect of 6-methyl-3-(thietan- 3-yl)pyrimidine-2,4(1H,3H)-dione on the generation of reactive oxygen species (ROS) in the whole blood and bone marrow using the study of whole blood spontaneous and stimulated chemiluminescence (CL). CL methods make it possible to quickly and easily assess the studied material (whole blood, bone marrow) effect on free radical oxidation. Using CL methods, it is possible to reveal the presence of medicines' pro- or antioxidant properties, opening up new possibilities in the search for substances with antioxidant properties and comparing their activity. Alkylation of 6-methylpyrimidine-2,4(1H,3H)-dione by 2-chloromethylthiirane in protic solvents in the presence of alkali leads to the formation of an N-thietane derivative. NMR spectroscopy showed that 6-methylpyrimidine-2,4(1H,3H)-dione was alkylated at position 3. The oxidation reactions of N-(thietan-3-yl)pyrimidine-2,4(1H,3H)-dione were studied, and it was determined that, depending on the excess of the oxidizing agent and the duration of the process, N-(1-oxothietan-3-yl)- or N-(1,1-- dioxothietan-3-yl)pyrimidine-2,4(1H,3H)-diones were formed. The effects of free radical oxidation processes of new biologically active pyrimidine-2,4(1H,3H)-diones were studied. New pyrimidine-2,4(1H,3H)-diones increase the general adaptive capabilities of the body and have protective effects in extreme conditions.

STRESS-PROTECTIVE ACTIVITY OF THE COMPLEX REMEDY IN ACUTE IMMOBILIZATION STRESS

Relevance. The range of medicinal remedies in the traditional Tibetan medicine contains several tens of formulae named bchud len. They are multicomponent remedies of natural origin having a stimulating non-specific effect on the human body. The analysis of such formula under the name ‘Rejuvenating-35’ has been presented in the paper. The complex remedy on the base of this formula was developed. Its composition includes 14 species of the plants and chitosan as a source of mineral substances. Previous phytochemical studies have revealed that the given remedy contains a wide spectrum of biologically active substances, namely triterpene saponins, flavonoids, ether oils, tannins, polysaccharides, etc. The aim of the present work was to evaluate stress-protective activity of the complex remedy in acute restraint stress. Material and methods. Experiments were carried out on white Wistar rats. The acute stress was simulated by immobilization of animals in supine position for 18 hours. The tested remedy was introduced in the form of decoction for 7 days before exposure to stress. Results. It has been established that the course administration of the complex remedy decreases the manifestation of the Selye’s triad signs: 26% decrease of adrenal glands hypertrophy, 45% and 60% decrease in involution of thymus and spleen respectively as compared to the data in the control group of animals. Besides, its administration prevents the development of rough destructions in the stomach mucosa in the form of erosions and linear ulcers. Against the background of the complex remedy administration there was noted twofold decrease of TBA-active products concentra-tion, fourfold increase of superoxide dismutase activity, 30% increase of catalase activity and the content of the reduced glutathione was 30% in-creased as compared to the data in the control. Conclusions. The preventive administration of the complex remedy has stress-protective effect against the background of acute restraint stress. Molecular-cell mechanisms of its anti-stress effect are connected with the inhibition of free-radical oxidation processes and the increased activity of the endogenic antioxidant system.

Innovative screening for functional improved aromatic amine derivatives: Toxicokinetics, free radical oxidation pathway and carcinogenic adverse outcome pathway

Aromatic amines, one of the most widely used low-cost antioxidants in rubbers, have been regarded as pollutants with human health concerns. To overcome this problem, this study developed a systematic molecular design, screening, and performance evaluation method to design functionally improved, environmentally friendly and synthesizable aromatic amine alternatives for the first time. Nine of 33 designed aromatic amine derivatives have improved antioxidant property (lower bond dissociation energy of N-H), and their environmental and bladder carcinogenicity impacts were evaluated through toxicokinetic model and molecular dynamics simulation. The environmental fate of the designed AAs-11–8, AAs-11–16, and AAs-12–2 after antioxidation (i.e., peroxyl radicals (ROO·), hydroxyl radicals (HO·), superoxide anion radicals (O2·-) and ozonation reaction) was also analyzed. Results showed that the by-products of AAs-11–8 and AAs-12–2 have less toxicity after antioxidation. In addition, human bladder carcinogenicity of the screened alternatives was also evaluated through adverse outcome pathway. The carcinogenic mechanisms were analyzed and verified through amino acid residue distribution characteristics, 3D-QSAR and 2D-QSAR models. AAs-12–2, with high antioxidation property, low environmental impacts and carcinogenicity, was screened as the optimum alternative for 3,5-Dimethylbenzenamine. This study provided theoretical support for designing environmentally friendly and functionally improved aromatic amine alternatives from toxicity evaluation and mechanism analysis.

Simultaneous remediation of co-contaminated soil by ball-milled zero-valent iron coupled with persulfate oxidation

The problem of co-contaminated soil at e-waste dismantling sites is serious and constitutes a critical threat to human health and the ecological environment. Zero-valent iron (ZVI) has been proven to be effective in the stabilization of heavy metals and the removal of halogenated organic compounds (HOCs) from soils. However, for the remediation of co-contamination of heavy metals with HOCs, ZVI has disadvantages such as high remediation cost and inability to take into account both pollutants, which limits its large-scale application. In this paper, boric acid and commercial zero-valent iron (cZVI) were used as raw materials to prepare boric acid-modified zero-valent iron (B-ZVIbm) through a high-energy ball milling strategy. B-ZVIbm coupled with persulfate (PS) to achieve simultaneous remediation of co-contaminated soil. The synergistic treatment of PS and B-ZVIbm resulted in the removal efficiency of 81.3% for decabromodiphenyl ether (BDE209) and the stabilization efficiencies of 96.5%, 99.8%, and 28.8% for Cu, Pb, and Cd respectively in the co-contaminated soil. A series of physical and chemical characterization methods showed that the oxide coat on the surface of B-ZVIbm could be replaced by borides during ball milling. The boride coat facilitated the exposure of the Fe0 core, promoted the corrosion of ZVI and the orderly release of Fe2+. The analysis of the morphological transformation of heavy metals in soils revealed that most of the heavy metals in the exchangeable, carbonate-bound state were transformed into the residue state, which was the key mechanism for the remediation of heavy metal-contaminated soils with B-ZVIbm. The analysis of BDE209 degradation products showed that BDE209 was degraded to lower brominated products and further mineralized by ZVI reduction and free radical oxidation. In general, B-ZVIbm coupled with PS is a good recipe for synergistic remediation of co-contaminated soils with heavy metals and HOCs.

РОЛЬ ПРОЦЕССОВ СВОБОДНО-РАДИКАЛЬНОГО ОКИСЛЕНИЯ В ПАТОГЕНЕЗЕ НЕВЫНАШИВАНИЯ БЕРЕМЕННОСТИ РАННИХ СРОКОВ

Currently, despite the already known pathogenic factors and mechanisms of miscarriage, about 50 % of ones continue to be unspecified or idiopathic. In recent years, the role of oxidative stress in the pathogenesis of many diseases, including the female reproductive system and pregnancy complica-tions, has been actively discussed. The purpose of the review is to analyze and provide information on the role of free-radical oxidation processes in the development and course of a pregnancy, both in normal and in miscarriage, as well as on the possible use of oxidative stress biomarkers in practical medicine, in order to assess the nature of the pathological process and the effectiveness of treatment. Understanding the processes of cell damage caused by oxidative stress can provide invaluable assis-tance not only in developing a personalized approach to each specific case of miscarriage, but also to expand knowledge, both scientific and clinical in this area of medicine.

Antidiabetic potential of Chia (Salvia hispanica L.) Seed Oil in streptozotocin induced diabetic rat

Chia seed oil (CSO) is a natural carotenoid that has active pharmacological properties and has been the most popular in recent years. The aim this study was to investigate the hypoglycemic antioxidative/nitrosative, oxidative DNA damage and adenosine deaminase effects of chia seed oil on streptozotocin induced diabetes in rats. Sprague Dawley, adult, male rats were used in the study. Animals were divided into the following groups: Group I (Control, n=7), Group II (Chia Group, Chia seed oil (CSO) was applied by oral gavage at 1g/kg for 14 days, n=7), Group III ((Diabetes (DM) Group, Streptozotocin (STZ) was applied as 50 mg/kg i.p. single dose (n=7)), and Group IV (DM+CSO Group, STZ 50 mg/kg i.p. single dose +CSO was applied by oral gavage at 1g/kg for 14 days after diabetes was induced, (n=7)). Physiological parameters (body weight, blood glucose) were evaluated. STZ exposure increased serum TOS, OSI and NO levels, which are markers associated with oxidative damage, while PON enzyme activities decreased compared to the control group and did not change TAS levels. Moreover, 8-OHdG level and ADA activity were increased in the diabetic groups. In addition, it was found to regulate body weight and increased glucose in the diabetic group.Our results show that Chia treatment effectively counteracts oxidative stress-mediated free radicals damage caused by diabetes and may serve as a therapeutic agent in reducing oxidative damage in diabetic subjects.

Influence of alginate-fabric coating with silver nanoparticles on the course of wound process in laboratory animals

Alginates with various modifying additives (for example, chitosan) and various methods of physical treatment are used in the design of prototypes of innovative wound dressing. The aim of this study was to create and explore the properties of a prototype of textile-based sodium alginate wound dressing containing silver nanoparticles subjected to10 freeze cycles, and to evaluate its efficacy on the course of the purulent wound process in rats. The study showed that the developed technology of 10-fold cyclic freezing makes it possible to significantly increase the amount of silver nanoparticles in the composition of textile-based sodium alginate wound dressing, primarily due to silver nanoparticles that have a diameter not larger than 15 nm, and are characterized by the highest antibacterial activity. The use of the developed wound dressing with silver nanoparticles reduces metabolic disturbances when the nonspecific defense system response occurs in the wound tissue; on the 3rd and 5th day after wounding less pronounced changes in free radical oxidation indices were found, the levels of antioxidant defence enzymes (catalase and superoxide dismutase) in wound tissue were lower than those observed after the use of wound dressing with silver nanoparticles, but not containing alginate, or after wound debridement without alginate or nanoparticles done every day.

CORRECTION OF CHANGES IN LIPID METABOLISM AND REDOX SYSTEM IN PATIENTS WITH STEMI IN THE SETTING OF INSULIN RESISTANCE

Myocardial infarction (STEMI) remains one of the most important problems of modern Cardiology both in Ukraine and worldwide due to its recalcitrant indices of incidence, disability and mortality. The prevalence of metabolic syndrome (MS) and DM2 in Ukraine is quite high, accounting for 20-28% in the general population and over 50% in certain demographics. In persons with abdominal obesity, metabolic syndrome and DM2, there is a 30% total risk of developing MI in the next 8 years, and their mortality rate is double thatof persons without metabolic disorders. The unfavorable prognosis in such patients is attributed to insulin resistance as an additional risk factor for CAD progression and the development of ACS, which determines the need to develop methods for correction of these abnormal processes.
 The aim of the research: to explore the efficacy of correcting the disorders of lipid profile and prooxidant/antioxidant system balance in patients with STEMI in a setting of insulin resistance and high risk of reperfusion complications by using dapagliflozin, a SGLT2 inhibitor.
 The methods of the research. The basis of the work has been underlain by the analysis of a comprehensive assessment and surgical treatment (percutaneous coronary intervention) in 73 patients with STEMI in the setting of insulin resistance in MS and DM2. The parameters of lipid profile and the activity of the components in the prooxidant/antioxidant system were determined by spectrophotometric methods; the absorbancy was measured on a Biomat 5 spectrophotometer (United Kingdom).The diagnosis of ACS was verified according to ESC Guidelines in the presence of a typical anginal attack, ACS-specific ECG changes with time (reciprocal ST displacement) and the signs of necrosis-resorption syndrome.The diagnosis was subsequently confirmed by the results of urgent coronary angiography. The diagnosis of MS and type 2 DM was made according to the recommendations of the WHO Expert Committee (2013) and the updated ADA/EASD consensus (2018).
 Results. In order to improve lipid profile and redox system balance in pre- and postoperative period, the patients were prescribed dapagliflozin at the dose of 10 mg/day, which allowed compensation of hyperglycemia, reduction of insulin resistance and stabilizing the activity of prooxidant/antioxidant system, preventing post-PCI reperfusion complications and stabilizing the clinical condition of comorbid patients in the postoperative period owing to a 40.4% reduction in the incidence of rhythm and conductivity disorders, and a 55.3% reduction in manifestations of acute heart failure. There has been a substantial reduction in free radical oxidation in the setting of dapagliflozin treatment, as suggested by a 1.3-fold reduction (р<0.05) in active products of thiobarbituric acid in the serum and a simultaneous restoration of functioning of enzymatic antioxidant systems, a 1.5-fold reduction (p<0.05) in the degree of superoxide dismutase blockage with an increased activity of catalase and ceruloplasmin.
 Conclusions. The use of dapagliflozin in pre- and postoperative patients with STEMI in the setting of insulin resistance and high surgical risk contributes to improvement of lipid metabolism and a significant reduction in the activity of free radical oxidation with restoration of functioning of enzymatic antioxidant systems in the body, which allows for a significant reduction in the incidence of rhythm and conduction disorders and the manifestations of acute heart failure.

Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination.

Phenolic acid is a well-known allelochemical, but also a pollutant in soil and water impeding crop production. Biochar is a multifunctional material widely used to mitigate the phenolic acids allelopathic effect. However, phenolic acid absorbed by biochar can still be released. In order to improve the removal efficiency of phenolic acids by biochar, the biochar-dual oxidant (BDO) composite particles were synthesized in this study, and the underlying mechanism of the BDO particles in ameliorating p-coumaric acid (p-CA) oxidative damage to tomato seed germination was revealed. Upon p-CA treatment, the BDO composite particles application increased the radical length, radical surface area, and germination index by 95.0%, 52.8%, and 114.6%, respectively. Compared to using biochar or oxidants alone, the BDO particles addition resulted in a higher removal rate of p-CA and produced more O2•-, HO•, SO4•- and 1O2 radicals via autocatalytic action, suggesting that BDO particles removed phenolic acid by both adsorption and free radical oxidation. The addition of BDO particles maintained the levels of the antioxidant enzyme activity close to the control, and reduced the malondialdehyde and H2O2 by 49.7% and 49.5%, compared to the p-CA treatment. Integrative metabolomic and transcriptomic analyses revealed that 14 key metabolites and 62 genes were involved in phenylalanine and linoleic acid metabolism, which increased dramatically under p-CA stress but down-regulated with the addition of BDO particles. This study proved that the use of BDO composite particles could alleviate the oxidative stress of phenolic acid on tomato seeds. The findings will provide unprecedented insights into the application and mechanism of such composite particles as continuous cropping soil conditioners.

Comparative evaluation of the antioxidant activity, trace elements, and phytochemical analysis of the extracts of unripe plantain whole fruit and pulp

Plantains make a substantial contribution to food security and malnutrition eradication, thus making them a staple food in several tropical and subtropical climates. This research determined the mineral contents, antioxidant capacities and phytochemical constituents of plantain whole fruit (pulp and peel) and pulp extracts. To this end, the plantain samples were evaluated for their phytochemicals, mineral element content, and in vitro antioxidant properties. The phytochemical screening showed that the plantain whole fruit and pulp extracts contained phenols, terpenoids, flavonoids, cardiac glycosides, reducing sugars, alkaloids, and steroids. Tannins, on the other hand, were detected only in the plantain whole fruit extract. The mineral elements in the whole fruit were higher than those in the pulp, especially for iron, sodium, calcium, phosphorus, zinc, and copper. Among the mineral contents, sodium was predominant. In comparison to the plantain whole fruit extract, the pulp showed better scavenging activity for nitric oxide, DPPH, and hydroxyl free radicals. In conclusion, the findings revealed that unripe plantain whole fruit and pulp extracts may be a promising source of critical nutrients and bioactive substances.

AKTIVITAS ANTIOKSIDAN PADA EKSTRAK ETANOL 96% ANGGUR LAUT (Caulerpa racemosa) YANG BERASAL DARI PANTAI CIMANDIRI, KABUPATEN LEBAK-BANTEN

Seaweed is known to have the ability as an antioxidant, antibacterial, and immunostimulant. Antioxidants are compounds that can inhibit free radical oxidation reactions. This study aimed to determine the antioxidant activity of 96% ethanol extract of sea grapes (C.racemosa). Sea grapes extract (C.racemosa) for extract characteristics (moisture content and ash content), phytochemical analysis, heavy metal test, and antioxidant analysis using the DPPH method. The results of the characteristic test of the extract obtained water content (14.49%) and ash content (0.30%), these values ??did not exceed the standard extract values. The results of the phytochemical analysis showed that 96% ethanol extract of sea grapes (C.racemosa) contained steroid/triterpenoid compounds, flavonoids, saponins, and alkaloids. The levels of heavy metals found were still within safe limits, namely, lead (Pb) (TTD), mercury (Hg) (<0.001 ppm), Arsenic (As) (<0.001 ppm), and Cadmium (Cd) (0.005 ppm). The test results showed that the sea grape (C.racemosa) used was safe and free from heavy metal contamination. The results of antioxidant analysis on 96% ethanol extract of sea grape (C.racemosa) obtained an IC50 value of 237.51 mg/L. The IC50 value for quercetin is 9.52 mg/L. The results of antioxidant analysis on 96% ethanol extract of sea grapes (C.racemosa) were included in the moderate antioxidant range (100-250 mg/L). Sea grapes (C.racemosa) have the potential as a natural antioxidant.
 

The influence of the feed additive “Sylimevit” on the antioxidant protection of the body of dogs

Under the influence of the pathological factor, there is a change in the intensity of lipid peroxidation, the accumulation in the blood of the concentration of products of free radical oxidation and reactive oxygen species, a decrease in the buffer capacity of the blood relative to the maintenance of optimal parameters of the intensity of free extreme reactions. The work aimed to investigate the effect of the feed additive “Sylimevit” on the antioxidant protection of the body of dogs after preventive deworming. 10 German Shepherd dogs aged 1–2 years were used for experimental research. It was established that when feeding the feed additive “Sylimevit” to dogs in the period after deworming, activation of the enzyme link of the antioxidant system occurs in the blood, which is indicated by an increase in the activity of catalase and superoxide dismutase in the blood serum of these animals and inhibition of lipid peroxidation processes (reduction of secondary and end products lipid peroxidation). On the 30th day of the experiment, the activity of catalase and superoxide dismutase in the blood of animals of the experimental group was the highest, and the level of lipid peroxidation products was the lowest. The obtained research results confirm the antioxidant properties of the Sylimevit feed additive. This may be because the composition of the drug includes milk thistle, methiphene, and vitamins, which in turn enhance the effect of each other and thereby inhibit the formation of radicals and the processes of lipid peroxidation. It is also worth noting the antioxidant properties of milk thistle, which according to the literature, also have similar properties. The complex effect of the indicated biologically active elements provided high hepatoprotective and antioxidant effects.