Free Radical Oxidation Processes Research Articles

Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

Hepatic manifestations of metabolic syndrome in boys with hypoandrogenism

Objective — to examine the morpho-functional state of the liver and the processes of free radical oxidation in boys with hypoandrogenism.Materials and methods. The study involved 47 adolescents with hypoandrogenism aged 13—18 years, who underwent a set of standard and special clinical and laboratory examination on the basis of the department of endocrinology SI «Institute of Children and Adolescents Health Care of the NAMS of Ukraine». Patients with hypoandrogenism were divided into groups depending on the index of masculinization. Mathematical processing of the study results was carried out using software packages SPSS Statistics 17.0, Excel.Results and discussion. It was found that complaints of abdominal pain and dyspeptic symptoms were observed in 62 % of cases. The conventional biochemical testing of liver function determined that the average levels of total bilirubin and liver enzymes were within normal range, suggesting that boys with hypoandrogenism probably had no significant violations of the functional state of the liver yet. Analysis of the lipid profile of children and adolescents with hypoandrogenism revealed that 75 % of patients had pathological changes in lipid levels, including 85 % with increased total cholesterol, 34 % with decreased HDL cholesterol and increased TG, 34 % with increased LDL cholesterol, 36 % with increased atherogenic coefficient. Atherogenic­chan­ges in the lipidogram increased with age. The study of indicators of free radical oxidation process revealed that in boys with hypoandrogenism the level of the final product of lipid peroxidation — malondialdehyde remained at the level of the control group, and the level of carbonated proteins tended to decrease with the increasing in the degree of delayed puberty. A significant reduction of the glutathione peroxidase level respectively to the deficiency of androgens was found. Thus, in adolescents with hypoandrogenism, a tendency towards the formation of the state of oxidative stress was revealed, which is confirmed by an increase of the integral coefficient of oxidative stress. Reducing the activity of the most powerful part of antioxidant system, in particular enzymatic, can lead to a «second hit» in the formation of non­alcoholic fatty liver disease and contribute to the transformation of liver steatosis into steatohepatitis. The results of ultrasound examination indicated the presence of functional disorders of the biliary tract in all boys with hypoandrogenism, and in one third of patients there were clear signs of steatohepa­tosis and gallstone disease. Conclusions. The results of the study indicated the formation of comorbid pathology of the hepatobiliary system in boys with hypoandrogenism. In 30 % of patients, ultrasound signs of steatohepatosis, atherogenic dyslipidemia and inhibition of antioxidant defense in the form of a significant decrease in the activity of glutathione peroxidase were detected. The results of the study indicate the necessity of inclusion in the diagnostic algorithm in boys with hypoandrogenism indicators of lipidogram, ultrasound of the liver and biliary tract, as well as the development of protocols for the prevention of hepatobiliary pathology, including the use of dietary correction, the prescription of hepatoprotectors with antioxidant activity, choleretic drugs.

Assessment of the condition of pro-/antioxidant systems in the rats heart of different age with hypogonadism

A specific androgen receptor is present in cardiomyocytes. Тhis proves the role of androgens in the work of the heart. Hypoandrogenia can be one of the conditions for changing the intensity of the processes of radical formation and the activity of the antioxidant system in the cells of the heart. Such changes may cause metabolic disorders and may increase the risk of developing and progressing pathological changes in the cardiovascular system. The age characteristics of the oxidative balance in the heart of different ages rats are determined. It is proved, that the influence of hypoandrogenism on the state of pro- / antioxidant systems in the heart of rats is age-dependent. It was found that the condition of androgen deficiency is more reflected on the oxidative balance of the heart of early sexual maturity rats. It has been established that hypoandrogenism is accompanied by modulation of the state of pro- / antioxidant balance in the heart of 3-months-old animals. This leads to the formation of oxidative stress. At the same time, in the heart of 2-months-old rats, under conditions of hypoandrogenism, there are significant changes in the intensity of the processes of free radical oxidation of proteins and lipids, as well as the activity of antioxidant defense enzymes. does not occur due to the fact that the rats in this age have increased basal indicators of redox-balance. It is suggested that testosterone in the heart of pubertal rats is not a key factor in the processes of regulation of the oxidative balance, where as in early sexual maturity age, its properties change towards the antioxidant effect.

Состояние процессов антиоксидантной защиты и свободнорадикального окисления в печени крыс в условиях гипоандрогении

Состояние процессов антиоксидантной защиты и свободнорадикального окисления в печени крыс в условиях гипоандрогении

THE INFLUENCE OF THYROID HORMONES ON PROTEIN OXIDATIVE MODIFICATION IN CASE OF EXPERIMENTAL PERIODONTITIS

Introduction. The inflammatory-dystrophic process in periodontium is accompanied by its hypoxia, which causes the activation of free radical oxidation processes. Tissue destruction in periodontal diseases is considered to be the result of an altered inflammatory/immune response to microbial plaque and involves massive release of neutrophils, reactive oxygen species and enzymes.
 The aim of the study – to establish the protein oxidative modification in blood and periodontium in case of periodontitis combined with hyper- and hypothyroidism in rats.
 Research Methods. Experimental studies were carry out on 48 mature male, nonliner, white rats, which were divided into the following groups: І – control animals; ІІ – animals with periodontitis; ІІІ – animals with periodontitis combined with hyperthyroidism; IV – animals with periodontitis combined with hypothyroidism. Protein oxidative modification (POM) was determined in blood serum and periodontium homogenate by the method of I.F. Meschyshyn.
 Results and Discussion. The results of our studies showed that the intensity of the processes of protein oxidative modification was significantly increased in animals of all experimental groups vs control group. The content of aldehyde- and ketone derivatives of the alkali nature changed more pronounced than the content of aldehyde and ketone derivatives of neutral nature.
 Conclusions. Thus, the experimental periodontitis is accompanied by a marked increase in the intensity of the protein oxidative modifications both in the homogenate of periodontal tissues and in the blood serum. Imbalance of thyroid hormones increases oxidative stress in experimental periodontitis, especially pronounced in hyperthyroidism.

Dynamics of free-radical oxidation in suppurative otitis media

The dynamics of indicators of free-radical oxidation in various purulent-inflammatory diseases of the middle ear in 280 patients was studied. Significant violations of the processes of free radical oxidation in the serum of blood were taken for the patients, the degree of which depends entirely on the severity and nature of the inflammation. A complex treatment with the use of antioxidant therapy in 280 patients was carried out. Positive results were obtained, confirmed by clinical and laboratory indicators. The pathogenetic significance and dependence of the course of purulent-inflammatory diseases of the middle ear on the processes of free radical oxidation was established, which was reflected in the determination of the duration of treatment of various forms of both acute and chronic otitis media.

Оцінка стану перекисного окислення ліпідів методом хемілюмінесценції у самців кролів за гонадодистрофій

Reducing the reproductive capacity of males is one of the major problems in andrology. Poor quality of semen and its reproductive capacity, impaired structural and functional characteristics of sperm is a consequence of gonadodystrophy. The leading link in the pathogenesis of various types of gonadodystrophy is the violation of the prooxidant-antioxidant system. Early diagnosis of a complex of pathological processes that develop in gonadodystrophy is possible due to the registration of the intensity of the processes of free radical oxidation. It is known that a highly sensitive way of detecting subtle biochemical shifts is chemiluminescence, which is used to detect free radical oxidation, the development of generalized oxidative stress. The aim of the study was to estimate the state of lipid peroxidation in rabbits with gonadodystrophy by the chemiluminescent method. The main tasks of the study: to investigate the state of the lipid peroxidation system by determining the final product of peroxidation – malondialdehyde in the serum of rabbits with gonadodystrophy; to determine the intensity of rabbit serum chemiluminescence and to determine the use of this technique for the diagnosis of gonadodystrophy. Three groups were formed: control – clinically healthy animals kept on a standard diet; experimental group I – rabbits with alimentary type of gonadodystrophy (prolonged maintenance on a diet deficient in carotene (vitamin A), Zinc); experimental group II – rabbits with gonadodystrophy of toxic type (chronic nitrate-nitrite toxicosis, caused by feeding of sodium nitrate). Serum samples were taken in 15, 30, and 45 day of the study. The concentration of malondialdehyde was determined by a fluorimetry method based on the reaction between malondialdehyde and thiobarbituric acid, which occurs under conditions of high temperature and acidic environment, with the formation of a colored trimethin complex. Spectra of spontaneous chemiluminescence of serum samples were measured on a chemiluminometer "Lum-5773", manufactured by the Russian Federation, which was connected to an interface with a personal computer to record the instrument performance with PowerGraph software (version 3.3). The rate of light chemiluminescence was estimated in 5 minutes. The state of lipid peroxidation in rabbits according to the malondialdehyde content showed a significant increase in the indicator in the experimental groups I, II compared with the control. It was noted that in the animals of the experimental group I with gonadodystrophy of alimentary type there was increased number of malondialdehyde in the serum by 15 days (by 58,3 %, P ˃ 0.001), the figure reached the maximum value by 45 days of the experiment (increase by 76 %, P ˃ 0.001), however, there was a minimal difference between the 30 and 45 day samples. In serum samples of male rabbits with toxic gonadodystrophy, there was a marked increase in malondialdehyde concentration by the 15th day of the experiment (by 75 %, P ˃ 0.001), and by an increase in the number during the experiment (up to 96 % by 45 days, P ˃ 0.001). Data obtained from a chemiluminescent serum study had a similar upward trend as with malondialdehyde in both groups. This may indicate a lack of activity of the body antioxidant protection system and the need for antioxidant therapy, due to the complexity of the chemiluminescence indicator, which is a concomitant marker of free radical oxidation. Chemiluminescent analysis of serum samples of rabbit males showed high efficiency as a diagnostic tool for gonadodystrophy and showed similar results to the malondialdehyde determination. Obtained during chemiluminescent analysis, data indicate the need of therapeutic measures as early as the 15th day of the experiment, due to the significant accumulation of lipid peroxidation products and the lack of adequate response of the organism to the action of external factors.

Innovative approaches to identifying the relationship between the distribution of the concentration of enzymes in the blood serum and skeletal muscle tissue of rats

One of the reasons for the increase in disability and mortality among the working population is cardiovascular diseases. Long-term studies of the nature of diseases belonging to this group hypertension, renovascular and malignant arterial hypertension, dyslipidemia, diabetic and dilatational cardiomyopathy, atherosclerosis, ischemic heart disease show that the main cause of pathogenesis is the formation of oxidative stress under the influence of internal and external environmental factors. Mismatch between the processes of free-radical oxidation caused by such active oxygen species as superoxide-anion radical, hydroxide-radical radical, lipid radical, hydrogen peroxide, peroxynitrite, chloric acid and the capabilities of the antioxidant system leads to a disruption of the functional activity of cell structures, displacement of homeostatic balance in the body. Despite the rather deep study of cardiovascular system pathologies, data on the functional activity of myocardial cells under destructive effects and oxidative stress are still insufficient.The article presents the study of the interrelation of the concentration distribution of enzymes of the lipidantioxidant peroxidation system in blood serum and skeletal muscle tissue of white non-pedigree rats. By means of Spearman, Gamma and Kendel Tau correlation coefficients it was revealed a reliable presence of a weak force of inverse correlation between the concentration of catalase in blood serum and skeletal muscle (Spearman R = -0.21 at p < 0.011749, Gamma = -0.14 at p < 0.010977; Kendall Tau = -0.14 at p < 0.010977).

DEPENDENCE OF THE ACTIVITY OF THE SYSTEM OF OXIDATIVE STRESS – ANTIOXIDANT PROTECTION ON INSULIN RESISTANCE IN PATIENTS WITH ESSENTIAL HYPERTENSION AND OBESITY

Relevance. The activation of free radical oxidative processes and the development of oxidative stress is one of the most important pathogenetic mechanisms of cardiovascular diseases. Active forms of oxygen can change cellular infiltration of vessels and endothelial function, having an impact on a functional condition of the adhesive molecules intercellular adhesion molecule-1 and vascular cell adhesion molecule-1. Under condition of insulin resistance (IR), the balance in the system of oxidative stress ­–­ antioxidant protection is disturbed, the result of which is insufficient resistance to the damaging effect of the LPO products. The aim of the study was to assess the dependence of the activity of the system of oxidative stress – antioxidant protection on insulin resistance (IR) in patients with comorbidity of arterial hypertension and obesity. We examined 200 patients with hypertension and class I–II obesity. The patients were divided into two groups depending on IR: the first group included 80 patients without IR and the second group – 120 patients with IR. As the result of the study, it was established that the presence of IR affects the activity of the system of oxidative stress – antioxidant protection in comorbidity of hypertension and obesity. In the presence of IR, patients with hypertension and concomitant obesity have significantly higher levels of indicators of the system of oxidative stress (malondialdehyde (MDA) and diene conjugates (DC)) compared with patients without IR. In patients with comorbidity of hypertension and obesity, HOMA-IR directly correlated with the indicators of the oxidative stress system (MDA and DC) and inversely correlated with the indicator of total antioxidant protection. In hypertensive patients with obesity and no IR, an increase in HOMA-IR was associated with a decrease in total antioxidant protection, and in patients with IR, an increase in HOMA-IR was associated with an increase in MDA and DC, as well as a decrease in total antioxidant protection.

ACTIVITY OF FREE RADICAL OXIDATION AND CYCLOOXYGENASE EXPRESSION IN RATS KIDNEYS UNDER EXPERIMENTAL THERAPY OF OXALATE NEPHROLITHIASIS WITH TETRAPEPTIDE Leu-Ile-Lys-Met

The aim of the study was to investigate the effect of Leu-Ile-Lys-Met tetrapeptide on the activity of free radical oxidation (FRO) and the level of cyclooxygenase-1 (COX-1) and COX-2 expression in rat kidney with experimental oxalate nephrolithiasis. Material and methods . The experiments were carried out on 30 male rats Wistar runoff, divided into 3 groups: the comparison group (10 intact rats), the control group (6 weeks of experimental oxalate nephrolithiasis simulation, n = 10), the experimental group (6 weeks of experimental simulation oxalate nephrolithiasis + oral administration of Leu-Ile-Lys-Met tetrapeptide at a dose of 12 mg/kg from the 3rd to the 6th week, n = 10). The oxalate nephrolithiasis was simulated according to conventional ethylene glycol model. COX-1 and COX-2 concentration in rat urine at baseline and after 6 weeks of experimental nephrolithiasis was determined by enzyme immunoassay using the prostaglandin-endoperoxide synthase 1 and 2 (PTGS 1, 2) kit from «Cloud-Clone Corp.». In rat kidney homogenate, the activity indicators of FRO processes were determined by common methods. Results and discussion . At the background of the Leu-Ile-Lys-Met tetrapeptide, free radical kidney damage was weakened, which was manifested in the complete absence of morphological signs of nephrolithiasis, decrease of thiobarbituric acid-reactive product concentration and overall prooxidant activity as compared to the control group; glutathione peroxidase activity, on the contrary, increased by 1.5 times.

THE INTENSITY OF OXIDATIVE STRESS AND THE STATE OF THE FACTORS OF ANTIOXIDANT PROTECTION IN PATIENTS WITH NONALCOHOLIC STEATOHEPATITIS THROUGH THE PRISM OF COMORBIDITY WITH OSTEOARTHRITIS AND OBESITY

Objective. To investigate the pathogenetic role of processes of free radical oxidation of lipids (FROL), oxidative modification of proteins (OMP) and factors of antioxidant defense (AOD) in development and mechanisms of mutual burden in patients with nonalcoholic steatohepatitis in the context of comorbidities with osteoarthritis and obesity. Material and methods . 90 patients were divided into three groups: group I (n = 30) patients suffering from OA II-III for Kellgren and Lawrense with normal body mass (BMI = 21-25 kg / m 2 ), group II (n = 30) - patients with NASH and obesity without OA (BMI> 30 kg / m 2 ), group III (n = 30) - patients with OA with NASH and obesity (BMI more than 30 kg / m 2 ). The control group consisted of 30 healthy individuals of the corresponding age. The average age of patients was (62,3 ± 5,7) years. Results. The peculiarity of the clinical course of NASH against the background of obesity and OA is the highest among the groups of comparing growth of MA content in plasma and Er - respectively in 1,97 and 2,12 times (p<0,05) compared with the normative ones. For patients with nonalcoholic steatohepatitis, obesity and osteoarthritis, the following changes in the content of intermediate molecular products of POL in the blood were: a possible increase in the content of IDB in the blood in 2,44 times; DC- in 2,92 times and the content of KCT in the blood – in 4,14 times (p <0,05). In addition, a probable increase in OMP intensity was found: the content of AKDNFHNC and AKDNFHMC exceeded the values in 2,40 and 1,85 times (p <0,05) respectively. In assessing the state of functioning of the AOP system for the comorbid flow of NASH, OB and OA, the maximum reduction in the content of GR - in 1,86 times (p <0,05) was established. Regarding the functioning of the glutathione dependent enzyme (glutathione peroxidase), the expected maximum increase in the enzyme activity was confirmed in 1,72 times (p <0,05). In the course of the correlation analysis between the indicators of the intensity of the POL and the main markers of cytolytic, cholestatic, mesenchymal - inflammatory syndromes, the presence of direct communication has been established, the inverse correlation connection is between the content of glutathione recovered and markers of the above syndromes. Conclusion. As a result of the study, it was found that the comorbidity of NASH with OB and OA increases the intensity of oxidative stress. The uncontrolled intensification of the processes of free radical oxidation of lipids and proteins in patients with non-alcoholic steatohepatitis, which developed on the background of obesity, determines the degree of activity of the pathological process in the liver.

Regularities in disturbances of cytological composition of synovial fluid and activity of free radical oxidative processes in case of experimental joint injury and possibility of their pathogenetic correction

Regularities in disturbances of cytological composition of synovial fluid and activity of free radical oxidative processes in case of experimental joint injury and possibility of their pathogenetic correction

FREE-RADICAL OXIDATION AND OXYGENATION OF HEMOGLOBIN AT EXACERBATION OF CYTOMEGALOVIRUS INFECTION IN THE SECOND TRIMESTER OF PREGNANCY

The aim of the study was to assess free radical processes and their effect on oxygenation of hemoglobin in the blood of 18-21 weeks pregnant women with exacerbation of cytomegalovirus infection. We examined 40 pregnant women with exacerbation of cytomegalovirus infection and various levels of specific antibodies (IgM) at 18-21 weeks and 30 pregnant women without cytomegalovirus. The spectrophotometric method was used to determine the content of oxyhemoglobin, methemoglobin, thiobarbituric acid (TBA)-active products, superoxide dismutase in blood erythrocytes; thin layer chromatography – phosphotidylcholine, lysophosphatidylcholine; gas-liquid chromatography – arachidonic acid. Exacerbation of cytomegalovirus infection changes the activity of free radical oxidation processes, the severity of which is determined by the level of IgM antibodies and is manifested by a decrease in superoxide dismutase (p = 0.000) and phosphatidylcholine (p = 0.000), an increase in phospholipase A2 (p = 0.000), lysophosphatidylcholine (p = 0.000), arachidonic acid (p = 0.000) and TBA-active products (p = 0.000). Accumulation of superoxide anion radical and products of lipoperoxidation in erythrocytes decreases oxyhemoglobin (p = 0.000) and increases methemoglobin (p = 0.000). Exacerbation of cytomegalovirus infection at 18-21 weeks is associated with the enhancement of free radical lipid oxidation and a deficiency in the antioxidant activity of superoxide dismutase. It decreases oxygen transport properties and increases phagocytosis by red blood monocytes; and pregnant women develop further hemic hypoxia.

The reasonableness of hormone replacement therapy in patients with stable angina and subclinical hypothyroidism living in iodine-deficiency regions

The purpose of research is to improve the quality and effectiveness of the treatment of patients with stable angina and subclinical hypothyroidism, living in iodine deficiency regions by complex treatment of dishormonal, dismetabolic, hemodynamic and autonomic disorders and differentiated inclusion in complex therapy low doses of L-thyroxine and L-carnitine in the background of receiving potassium iodide. Use of a standard anti-ischemic therapy and potassium iodide in patients with subclinical hypothyroidism and stable angina, living in areas of iodine deficiency, does not improve the laboratory and instrumental manifestations of comorbid pathology. The combination of anti-ischemic therapy and L-thyroxine has positive influence to the improvement of the free-radical oxidation processes and heart rate variability only in the group of patients with subclinical hypothyroidism and duration less than 5 years of stable angina. In the group of patients with a disease duration of more than 5 years for achievement of the target levels of the main indicators of the free-radical oxidation processes and antioxidant defense, as well as the normalization of hemodynamic and autonomic regulation of cardiac activity appropriate to apply combined therapy using a standard anti-ischemic treatment, potassium iodide, L-thyroxine and L-carnitine. Thus, for adequate restore of the clinical condition, hemodynamic parameters and to prevent metabolic disorders in patients with stable angina and subclinical hypothyroidism it is advisable to use a differentiated combination therapy by L-thyroxine and L-carnitine with based receivement potassium iodide.

ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF A SERIES OF NEW7,8-DISUBSTITUTED THEOPHYLLINE CONTAINING A PYRAZOLE RING

Objective: The objective of this study was to synthesize methyl 1-(1,3-dimethyl-2,6-dioxo-7-arylalkyl-(alkenyl-)-2,3,6,7-tetrahydro-1H-purin-8-yl)- 5-(4-methyl-(methoxy-, chloro-)phenyl)-1H-pyrazole-3-carboxylates and studying their antioxidant and anti-inflammatory properties by in vitro methods.Methods: New derivatives of 1,3-dimethylxanthine with pyrazole at position 8 were synthesized by the interaction of 8-bromotheophylline with arylalkyl-(alkenyl) halides, hydrazine hydrate, and methyl 4-(4-R)-2,4-dioxobutanoate. For most obtained compounds, antioxidant activity (by 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azobis(2-amidinopropane) dihydrochloride) and anti-inflammatory (by Soybean LOX inhibition) activity was investigated.Result: The most effect on free radical oxidation processes was recorded for substances with a phenylalyl radical at position 7 of the molecules (10, 13, and 16). In the activity of inhibition of peroxidation of lipids (89–92%), they predominate Trolox (88%). Their IC50 indices for LOX inhibition are 15.0, 17.5, and 27.5 μM, respectively, but less than the nordihydroguaretic acid reference compound used.Conclusion: The antioxidant and anti-inflammatory properties of a series of synthesized 7,8-disubstituted theophylline containing the pyrazole cycle have been studied using modern in vitro research methods. The prospect of conducting further synthetic and pharmacological studies for compounds with phenylalyl radical in the 7 positions of their molecules is shown.

STATUS OF FREE-RADICAL OXIDATION OF LIPIDS AND PROTEINS AND ENDOGENOUS INTOXICATION IN PATIENTS WITH ROSACEA

Objective – to identify and analyse the rates of free-radical oxidation (FRO) of lipids and proteins as well as of the endogenous intoxication in patients with rosacea depending on the clinical form and the duration of dermatosis course.Material and methods. The study involved 64 patients with rosacea (49 women and 15 men), aged 28 to 63. 23 of them were diagnosed with erythematous-telangiectastic and 41 with papulo-pustular stage (form) of rosacea. In 21 patients dermatosis lasted up to 6 months, in 22 – from 6 to 12 months and in 21 – more than 1 year. The status of free-radical oxidation of lipids and proteins was assessed by the content in plasma and in red blood cells of malonic aldehyde (MA) and the fractions of protein oxidative modification (POM Е370 and POM Е430) – in the blood serum, while the status of endogenous intoxication was evaluated by the content of medium mass molecules (МMМ Е254 and МMМ Е280) – in the serum according to available methods.Results. The patients with rosacea were found to have a reliable increase in the level of MA in plasma and in red blood cells (by 51,4 % and 17,3 % respectively, p&lt;0,001) in comparison with the control group, and in both fractions of protein oxidative modification POM Е370 and POM Е430 in the blood serum (in 1,75 and 2,23 times respectively, р&lt;0,001). The changes were more significant in patients with papulo-pustular stage (form) of rosacea than in those with erythematous-telangiectastic stage (form) of dermatosis. Patients with a duration of rosacea up to 6 months had a higher level of FRO of proteins, and those with more than a year - an increase in the incidence of FRO of lipids and manifestations of endogenous intoxication with an increase in the level of MMM.Conclusion. The patients with rosacea were found to experience an activation of the free-radical oxidation processes, which is characterized by more active oxidation of molecules in the early stages and with increasing term of dermatosis of lipid structures as well which is accompanied by more frequent events of endogenous intoxication and is more pronounced in patients with rosacea with papulo-pustular stage (form) of dermatosis than in those with erythematous-telangiectastic stage (form) of rosacea.

ВЛИЯНИЕ НИЗКОИНТЕНСИВНОГО КРАСНОГО СВЕТА НА ОКИСЛИТЕЛЬНУЮ МОДИФИКАЦИЮ БЕЛКОВ ПРИ РАЗВИТИИ РАДИАЦИОННО-ИНДУЦИРОВАННОЙ БОЛЕЗНИ ЛЕГКИХ В ЭКСПЕРИМЕНТЕ

The aim of the study was to investigate the content of spontaneous protein oxidative modification (POM) in the blood serum and pulmonary tissue of rats after local gamma irradiation of the projection area of the heart and lungs. The content of products of spontaneous POM (aliphatic neutral and basic aldehyde- and ketone-dinitrophenylhydrazones) in the blood serum and pulmonary tissue of 57 rats was studied. The animals were divided into 5 groups: “control group”, where there was gamma irradiation of the heart and lungs area, the collection of samples was done at the next day; “treatment group”, where a single session of low-intensity broadband red light was performed after local exposure to gamma radiation (the collection of samples was done at the next day); “chronic control group”, where there was an exposure to local gamma irradiation of the heart and lungs area, the collection of samples was done at the fourth day; “chronic treatment group” was daily exposed to low-intensity red light for 4 days after local exposure to gamma radiation, the collection of samples was done at the fourth day; “normal level group” was not exposed to either gamma radiation or broadband red light. In the pulmonary tissue and blood serum of control animals, a gradual increase in the content of spontaneous POM products was observed. The most significant difference from the normal level the “chronic control group” had. At the same time, the normalization of the content of POM products under the influence of low-intensity broadband red light in the tissues of the experimental groups was revealed. In the course of the experimental study, the normalization of processes of free radical oxidation of proteins in the lung tissue and in the serum of rats after gamma irradiation and the exposure of the projection region of the heart and lungs to low-intensity broadband red light was shown. Thus, low-intensity red light can be considered as a possible radioprotector.

METABOLIC CORRECTION OF CEREBRAL DISORDERS IN ACUTE OBSTRUCTIVE CHOLESTASIS (EXPERIMENTAL STUDY)

Aim. To research cerebral functional and structural disorders in acute obstructive cholestasis and possibilities of their metabolic correction. Material and Methods. Acute obstructive cholestasis was simulated by common bile duct compression in 26 anesthetized dogs which were divided into three series. The first group included cholestasis dogs without treatment. In the second group common bile duct (CBD) was decompressed in 3 days postoperatively and 0.9% sodium chloride solution (20 ml / kg) was administered for 5 days. In the 3 rd group Mexidol® (6.5 mg / kg) and Cortexin® (10 mg / kg) were injected additionally after CBD decompression. In blood plasma and cerebral homogenate malondialdehyde (MDA) and catalase levels were determined. Brain tissue histological investigation was performed with 200x and 400x magnification. Results. In the 1 st series on the 10 th day MDA and catalase levels in cerebral homogenate were 4.1 times higher and 4.3 times lower than those in control animals. Structural changes were presented by perivascular and pericellular edema, reduction of capillary volume, vacuolization and atrophy of neurons. In the 2 nd series there were 2.15-fold and 1.2-fold decrease of MDA in systemic circulation and in cerebral tissue respectively. Catalase activity was increased up to 1.36 and 1.22 times (p &gt; 0.05) accordingly. Cerebral structural disorders were the same. In the 3rd series there were 2.91-fold and 3.77-fold decrease of MDA in systemic circulation and in cerebral homogenate respectively in 10 days postoperatively. Catalase activity was increased up to 1.57 and 4.76 times (p &lt; 0.001) accordingly. Structural normalization was accompanied by increase of microcirculatory volume, elimination of vacuolization and atrophy of neurons, reduction of cerebral edema. Conclusion. Mexidol® and Cortexin® reduce significantly the processes of free-radical oxidation in the brain and contributes to normalization of structural disorders.

Intensification of processes of free radical oxidation and iron metabolism in children with medulloblastoma of the brain due to chemotherapy

Intensification of processes of free radical oxidation and iron metabolism in children with medulloblastoma of the brain due to chemotherapy

The influence of humus acids of peloids and its components on free radical processes

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Humic acids restore not only normal physiological redox systems, but also increase the activity of antioxidant enzymes on the 10th day.