METABOLIC CORRECTION OF CEREBRAL DISORDERS IN ACUTE OBSTRUCTIVE CHOLESTASIS (EXPERIMENTAL STUDY)

Abstract

Aim. To research cerebral functional and structural disorders in acute obstructive cholestasis and possibilities of their metabolic correction. Material and Methods. Acute obstructive cholestasis was simulated by common bile duct compression in 26 anesthetized dogs which were divided into three series. The first group included cholestasis dogs without treatment. In the second group common bile duct (CBD) was decompressed in 3 days postoperatively and 0.9% sodium chloride solution (20 ml / kg) was administered for 5 days. In the 3 rd group Mexidol® (6.5 mg / kg) and Cortexin® (10 mg / kg) were injected additionally after CBD decompression. In blood plasma and cerebral homogenate malondialdehyde (MDA) and catalase levels were determined. Brain tissue histological investigation was performed with 200x and 400x magnification. Results. In the 1 st series on the 10 th day MDA and catalase levels in cerebral homogenate were 4.1 times higher and 4.3 times lower than those in control animals. Structural changes were presented by perivascular and pericellular edema, reduction of capillary volume, vacuolization and atrophy of neurons. In the 2 nd series there were 2.15-fold and 1.2-fold decrease of MDA in systemic circulation and in cerebral tissue respectively. Catalase activity was increased up to 1.36 and 1.22 times (p > 0.05) accordingly. Cerebral structural disorders were the same. In the 3rd series there were 2.91-fold and 3.77-fold decrease of MDA in systemic circulation and in cerebral homogenate respectively in 10 days postoperatively. Catalase activity was increased up to 1.57 and 4.76 times (p < 0.001) accordingly. Structural normalization was accompanied by increase of microcirculatory volume, elimination of vacuolization and atrophy of neurons, reduction of cerebral edema. Conclusion. Mexidol® and Cortexin® reduce significantly the processes of free-radical oxidation in the brain and contributes to normalization of structural disorders.