The studies were carried out in field experiments in 2014-2017 with the aim of a comprehensive assessment of the action of bacterial bioagents on garden strawberry plants in the conditions of a production mother liquor. It has been established that preplant treatment of strawberry seedlings with a suspension of a mixture of strains of saprotrophic bacteria of the genus Bacillus (B. amyloliquefaciens VKPM V-10642, B. amyloliquefaciens VKPM V-10643, B. subtilis VKPM V-10641) in equal proportions, at a concentration of 1×105 CFU/ ml) increased their survival by an average of 20.5% relative to the control, providing a pronounced antistress effect. The degree of damage to strawberry leaves with white spotting decreased by 1.2-1.8 times due to the immunizing effect of the drug. Treatment with a mixture of bacterial strains stimulated an increase in the length of the aerial part by 15%, the length of the root system - by 19%, the biomass of strawberry plants - by 20%, as well as vegetative propagation of strawberries - the number of daughter rosettes increased by 31.8%. The preparative mixture of bacterial strains has a multifunctional effect on plants and is a promising tool for expanding the arsenal of biological methods to increase the efficiency and environmental stabilization of the production of strawberry planting material.