Micro Propagation on Strawberry: A Review

Abstract

The fruit crop known as strawberries (Fragaria ananassa) produces maximum revenues in the quickest period of time. It is the diet’s richest source of the vitamins and minerals needed for human health. The major method of growing strawberries is by runners, which produce susceptible-to-disease plants. Plantlets produced using in vitro micropropagation are free of disease and can be used for further culture. Using MS media supplemented with 3-4% sugar, 0.751.0% agar, and an adequate combination of plant growth hormones, such as 6-benzyladenine, NAA, IBA, and kinetin, shoot cultures can be grown from shoot tips. Strawberry explants have been cleaned, multiplied into shoots, rooted, and ex vitro acclimated as part of a routine regeneration technique. The difficulties in getting better-quality plants and their higher endurance rate during ex vitro acclimatization can be greatly reduced by in vitro micro propagation. The Culture of Tissue Laboratory, a division of Ain Shams University’s Faculty of Agriculture in Egypt, is where the study was conducted. This work’s main goal was to determine whether using runners as meristem cultures to micropropagate a strawberry cultivars Festival and Marquez is a feasible approach. This was done while testing various gibberellic acid (GA3) concentrations (i.e., 0.1, 0.2, 0.3, 0.4, and 0.5 mgl-1) during the multiplication phase. It was discovered that 0.4 mgl-1 of GA3 produced the greatest number of shoots each organ transplant and each shoot’s leaves, whereas 0.5 mgl-1 was the most effective focal point for growing buds.