Alcohol Fraction Research Articles

The dissolution behavior and thermodynamic properties analysis of pentoxyverine citrate in aqueous solutions of alcohols

The objective of this work is to study the dissolution behavior and thermodynamic properties of pentoxyverine citrate in aqueous solutions of alcohols. The results of solubility data at temperature range from 278.15 K to 318.15 K were obtained by the isothermal equilibrium method under atmospheric condition. In pure solvents, the largest solubility data in mole fraction is found in water at 318.15 K (2.756 × 10−2), followed by ethanol (1.156 × 10−2), n-propanol (8.129 × 10−3), isobutanol (5.233 × 10−3), isopropanol (4.340 × 10−3) and 1-butanol (3.435 × 10−3). The order of solubility from large to small is consistent with the order of solvent polarity. The solubility profile in mixtures of (ethanol + water), (n-propanol + water) and (isopropanol + water) increases firstly and then decreases with the increasing mass fraction of alcohols at all studied temperatures, and it reaches a maximum at w = 0.60 (the mass fraction of ethanol) in mixtures of (ethanol + water) at all investigated temperatures, for two other binary mixtures, the mass fraction of n-propanol and isopropanol is 0.40. The analysis of extended Hildebrand solubility approach indicate the solvent–solvent interactions and specific interactions are unfavorable for the dissolution process. The values of relative average deviation (RAD) for crystalline pentoverine citrate demonstrated well correlation of solubility values with Apelblat equation and Jouyban-Acree model. The positive values of ΔHsolo and ΔSsolo for dissolution behavior indicate the dissolution process was not only endothermic but also entropic-favouring. Moreover, the enthalpy is the main contributing force to the Gibbs free energy during the dissolution of pentoverine citrate.

Acyclic Diterpene Phytol from Hemp Seed Oil (Cannabis sativa L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages.

Seeds from non-drug varieties of hemp (Cannabis sativa L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes and human macrophages derived from circulating monocytes were used to evaluate both plasticity and anti-inflammatory effects of phytol from HSO at 10–100 mM using FACS analysis, ELISA, and RT-qPCR methods. The quantitative study of the acyclic alcohol fraction isolated from HSO shows that phytol is the most abundant component (167.59 ± 1.81 mg/Kg of HSO). Phytol was able to skew monocyte-macrophage plasticity toward the anti-inflammatory non-classical CD14+CD16++ monocyte phenotype and toward macrophage M2 (CD200Rhigh and MRC-1high), as well as to reduce the production of IL-1β, IL-6, and TNF-α, diminishing the inflammatory competence of mature human macrophages after lipopolysaccharide (LPS) treatment. These findings point out for the first time the reprogramming and anti-inflammatory activity of phytol in human monocyte-macrophages. In addition, our study may help to understand the mechanisms by which phytol from HSO contributes to the constant and progressive plasticity of the human monocyte-macrophage linage.

Interaction between an Oxidized Fraction of Polyvinyl Alcohol and Uracil Derivatives in Aqueous Solutions

Interaction between an Oxidized Fraction of Polyvinyl Alcohol and Uracil Derivatives in Aqueous Solutions

Fruit Microbial Communities of the Bisucciu Sardinian Apricot Cultivar (Prunus armeniaca L.) as a Reservoir of New Brewing Starter Strains

Local fruit cultivars may improve the originality of specialty beers both directly, by conferring peculiar tastes and flavors, and indirectly, as a reservoir of new starter strains. Accordingly, the fungal and bacterial communities of Bisucciu fruit, a Sardinian apricot cultivar used to produce a local fruit beer, were here investigated by culture-dependent methods. From the 16S rDNA and ITS sequence analyses of 68 epiphytic isolates, 5 bacterial species and 19 fungal species were identified. Aureobasidium pullulans and Rhodotorula glutinis were the dominant fungal species, while Enterococcus mundtii (Firmicutes) and Frigoribacterium faeni (Actinobacteria) were the most represented species among bacterial isolates. Enrichment cultures of fresh apricot puree, followed by fermentation trials in beer wort and beer wort added with apricot puree, allowed the selection of four isolates of Pichia kudriavzevii, Hanseniaspora uvarum, H. pseudoguilliermondii, and H. clermontiae able to dominate over the Bisucciu native microbiota and to produce from 0.57% to 0.74% (vol/vol) of ethanol. HS-SPME-GC/MS analysis highlighted a significant increase in the ester and alcohol fractions as well as a reduction in terpenes after fermentation with the selected yeasts. Results obtained suggest that the yeast isolates may contribute to the definition of the taste and flavor of beers when used in mixed fermentations with Saccharomyces.

Obtaining a Fraction of Sugarcane Wax Rich in Policosanol by Using Ethanol as Solvent: Results Interpretation through Hansen's Solubility Theory.

Determination of the best condition for fractionation of degreased sugarcane wax for policosanol production using ethanol was investigated in this paper. The optimal conditions related to the dispersion time of wax in the solvent, ethanol degree, and solvent/wax ratio were 30 min, 90.03% v/v, and 14:1 v/w, respectively. The results were evaluated by measuring six response variables: higher fatty alcohol concentration, octacosanol concentration, impurity concentration (measured as α,β unsaturated aldehydes), yield, cost indicator, and the ratio of octacosanol vs other higher fatty alcohols (C30 + C32 + C34). Optimal extraction conditions were determined with the desirability function. The complexity of separation of the higher alcohols fraction from impurities, mainly α,β unsaturated aldehydes, is explained with the aid of Hansen’s solubility parameters theory and its variation with temperature.

Cytoprotective effects and antioxidant activities of acteoside and various extracts of Clerodendrum cyrtophyllum Turcz leaves against t-BHP induced oxidative damage

This study evaluates the antioxidant potential and cytoprotective effects of ethanolic crude extract from Clerodendrum cyrtophyllum leaves (ECE) and five derived fractions (namely, petroleum ether fraction (PEF), dichloromethane fraction (DMF), ethyl acetate fraction (EAF), n-butyl alcohol fraction (BAF) and the remaining fraction (RF)), as well as acteoside (Ac, a major phenolic component in EAF) on oxidative damage caused by tert-butyl hydroperoxide (t-BHP) in HepG2 cells. MTT assay results showed that ECE, EAF, BAF, RF and Ac increased the viability of t-BHP-damaged cells in a dose-dependent manner, while EAF significantly promoted cell viability. EAF, BAF, RF, or Ac reduced the levels of lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA), and reactive oxygen species (ROS). Additionally, glutathione (GSH) levels and the activities of superoxide dismutase (SOD) and catalase (CAT) increased. Western blot analysis further indicated that EAF, BAF, RF, or Ac up-regulated pro-caspase-3 and reduced cleaved caspase-3 during t-BHP-induced oxidative stress. Flow cytometry analysis and fluorescence micrographs showed that Ac could inhibit apoptosis.

ПОВЫШЕНИЕ КАЧЕСТВЕННЫХ ХАРАКТЕРИСТИК СВЕТЛОГО ПИВА ПРИ ИСПОЛЬЗОВАНИИ НЕСОЛОЖЕНОЙ ОБЖАРЕННОЙ ГРЕЧИХИ

The possibilities of using vegetable raw materials to increase the organoleptic, physico‑chemical and microbiological qualities of light beer are presented. Investigation of the effect of enrichment of  beer with unsalted buckwheat in the production process on the dynamics of changes in the mass fraction of dry substances, on the dynamics of changes in the volume fraction of ethyl alcohol, as well as the physiological state of brewing yeast during the main fermentation. Based on the organoleptic evaluation, a positive result was revealed when adding unsalted buckwheat to beer

Abstract 707: Mahanine, a carbazole alkaloid attenuates lung cancer progression

Abstract Lung cancer continues to be a major cause of cancer mortality. Emerging evidence suggests that cancer likely involves multiple mutations. Therefore, compounds targeting multiple signal transduction pathways would be ideal for the prevention and treatment of cancer. Mahanine, a carbazole alkaloid isolated from an Indian folklore medicinal plant Murraya koenigii (known as Curry Patta), widely consumed in traditional dishes, have been shown to possess anticancer activities and confound many oncogenic signaling events in breast, colon, and prostate cancers. In the current study, we determined the anticancer potential of mahanine against drug-sensitive and drug-resistant lung cancer cells and postulate the possible mechanism of action. Mahanine was isolated from the dried leaves of Murraya koenigii by hydro alcoholic extraction and fractionation with ethyl acetate, followed by preparative UPLC to isolate pure (>95%) compound and its identity was confirmed by LC-MS/MS and NMR spectroscopy. Antiproliferative activity of mahanine was determined against drug- sensitive (A549 and H1299) and taxol-resistant (A549-TR) human lung cancer cells. Mahanine showed a dose- and time-dependent inhibition of both drug-sensitive and drug-resistant lung cancer cell growth. Interestingly, the IC50 values of mahanine were essentially the same for drug sensitive (A549) (12.5 µM) and drug- resistant (12.5 µM) lung cancer cells; the IC50 value was somewhat lower (10 µM) against highly metastatic H1299 lung cancer cells. Mahanine attenuated the colony formation and reduced the wound healing of lung cancer cells. Mechanistically, mahanine dose dependently impeded the oncogenic markers such as KRAS, MET, AKT, mTOR and cMYC. Together, mahanine demonstrate ability to inhibit both drug- sensitive and drug-resistant lung cancer cells and modulate distinct oncogenic molecular targets providing potential to be developed for the treatment of drug-resistant and metastatic NSCLC (Supported from Duggan Endowment funds). Keywords: Mahanine, Lung cancer, Oncogenes, Plant therapeutics. Citation Format: Raghuram Kandimalla, Farrukh Aqil, Disha N. Moholkar, Suman K. Samanta, Ramesh C. Gupta. Mahanine, a carbazole alkaloid attenuates lung cancer progression [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 707.

Construction of Synergistic Co and Cu Diatomic Sites for Enhanced Higher Alcohol Synthesis

Construction of Synergistic Co and Cu Diatomic Sites for Enhanced Higher Alcohol Synthesis

Preparation of Organic-Inorganic Coupling Phase Change Materials with Enhanced Thermal Storage Performance via Emulsion Polymerization.

The serious phase separation in inorganic phase change materials, and easy leakage of organic phase change materials are the main obstacles to the practical batch application of phase change heat storage materials. To solve these problems, in this work, emulsion polymerization is introduced as the method for preparing organic-inorganic coupling phase change material (oic-PCM) with high heat storage performance using polyacrylamide (PAM) as the wall material and organic phase change material of cetyl alcohol as the core material, and diatomite is used as a supporting substrate to absorb inorganic sodium sulfate decahydrate (SSD). A differential scanning calorimeter (DSC), X-ray diffractometer (XRD), dust morphology and dispersion analyzer, and thermal conductivity tester were used to characterize the prepared organic-inorganic coupled phase change materials and investigate their performance. The research results show that when the mass fraction of cetyl alcohol is 68.97%, the mass fraction of emulsifier is 3.38%, and the mass fraction of sodium sulfate decahydrate/diatomite is 3.40%. The phase change latent heat of the organic-inorganic coupled phase change material is as high as 164.13 J/g, and the thermal conductivity reaches up to 0.2061 W/(m·k), which proves that the prepared organic-inorganic coupled phase change material has good heat storage performance, showing its good application prospects.

Research on dissolution of actarit in aqueous mixtures: Solubility determination and correlation, preferential solvation, solvent effect and thermodynamics

The solubility information and dissolution mechanism of drugs in mixtures is significant in chemistry and pharmaceutical domains. The equilibrium saturated solubility of actarit in methanol + water, ethanol + water, 2-propanol + water and acetone + water in the temperature scope of 278.15–318.15 K was determined by using isothermal saturation method. The discussion of solvent effect, Hansen solubility parameters (HSPs), preferential solvation, thermodynamics and data correlation were performed. The solubility of actarit in mole fraction enhances as the increasing of temperature and mass fraction of alcohols in three alcohols-water mixtures, however, the solubility shown a cosolvency phenomenon at w = 0.80 in acetone + water mixtures. The solubility behavior and cosolvency phenomenon in actarit dissolution was discussed by HSPs in depth. The molecular interactions in solution were analyzed by KAT-LSER model of solvent effect. Furthermore, the preferential solvation mechanism of actarit in aqueous was obtained by IKBI method. In addition, the classical Jouyban-Acree model, Apelbat-Jouyban-Acree model and Van’t Hoff-Jouyban-Acree model were used to correlate the solubility data of actarit in four aqueous mixtures. The relative average deviation and root-mean-square deviation were used to evaluate the applicability of models. Finally, the apparent thermodynamics properties were calculated by Van’t Hoff analysis.

Antibacterial and antibiofilm properties of dichloromethane fraction of extracts from adventitious roots of Eurycoma longifolia against Staphylococcus aureus

Adventitious root (AR) cultures of Eurycoma longifolia are the alternative plant materials. However, the biological effects of the ARs are rarely investigated, leading to a delay in their practical application. In this study, antibacterial activities of sub-fractions of E. longifolia ARs extracted by different organic solvents were examined. Furthermore, antibacterial mechanism and antibiofilm effect of the dichloromethane fraction (DCM) against Staphylococcus aureus were explored. Results showed that petroleum ether, DCM, ethyl acetate, and n-butyl alcohol fractions inhibited growth of the tested bacterial species, and the highest inhibitory effect was observed in DCM against S. aureus with a minimum inhibitory concentration of 0.25 mg/mL. DCM promoted the leakage of cell contents of S. aureus, indicating cell permeability increased. After DCM treatment, S. aureus respiration was inhibited, and the activity of a key enzyme in hexose monophophate pathway (HMP) decreased, suggesting an involvement of DCM in regulating the HMP. DCM possessed the ability of scavenging S. aureus biofilm; nearly 50% biofilm was scavenged at 0.5 mg/mL DCM, at which the extracellular polysaccharides and proteins of the biofilm structure were reduced. The findings of this study indicate that DCM can be expected to be used in the production of food or pharmaceutical products.

Determining the double-bond positions of monounsaturated compounds in the alcohol fraction in seep carbonate

The unsaturation patterns of molecular fossils are critical in distinguishing their biological precursors and diagenetic processes. However, questions regarding the determination of double-bond positions of unsaturated dialkyl glycerol ethers (DAGEs) in submarine hydrocarbon seep ecosystems remain unsolved. To address this problem, a protocol for dimethyl disulfide (DMDS) derivative analysis using gas chromatography (GC)-mass spectrometry was optimised. Herein, the double-bond positions of monounsaturated short-chain alcohols, monoalkyl glycerol ethers (MAGEs), and DAGEs in seep carbonates were analysed. Among these compounds, the double-bond positions of trimethylsilyl (TMS) derivatives of the monounsaturated DAGE-DMDS adducts were determined for the first time, with mass spectra characterized by molecular ions (M+·) and two major diagnostic ions (ω+ and Δ+) cleaved at the double bonds. For both the MAGEs and DAGEs, the double-bond positions of the monounsaturated n-C16:1-alkyl moieties were identified at ω5 and ω7. Compared to monounsaturated short-chain alcohols and MAGEs, both ionization efficiency and relative sensitivity for the TMS derivatives of DAGE-DMDS adducts were low as indicated by the high limit of detection at a signal-to-noise ratio of 3. In addition, the appropriate injection parameters and oven temperature program during GC analyses may be crucial in determining the double-bond positions within monounsaturated DAGEs.

Methanol/Ethanol/Butanol–Gasoline Blends Use in Transportation Engine—Part 2: Composition, Morphology, and Characteristics of Particulates

Abstract Spark ignition (SI) engines have a remarkable share in the passenger car segment in urban areas worldwide. Particulate matter (PM) emitted from these vehicles has an adverse health impact since it can penetrate deep into the lungs, leading to cardiovascular issues. In this study, a detailed physical, chemical, and morphological characterization of the PM emitted by a production-grade SI engine fueled with different alcohol–gasoline blends (gasohols), namely GM10 (10% methanol blended with gasoline, v/v), GE10 (10% ethanol blended with gasoline, v/v), and GB10 (10% butanol blended with gasoline, v/v) vis-à-vis baseline gasoline was performed. Results exhibited that gasohol-fueled engines emitted relatively lower particulate mass and numbers. Trace metal analysis was performed to explore the composition of PM, which showed that gasoline-fueled engines exhibited comparatively higher emissions of trace metals than gasohol-fueled engines. PM morphology was also investigated to evaluate the suitability of alcohol blending with gasoline, revealing that even a small fraction of alcohols blended with gasoline could alter the structure of particulates. This study successfully demonstrated that the large-scale implementation of gasohols in SI engines could reduce PM emissions and trace metals in the particulates. This study also showed the potential of gasohols to partially replace fossil fuels, which could be explored further for higher alcohol fractions.

About the performance of using the extract of barley malt in the creation of modern drinks based on alcohol fermentation

It is known that scientific and technological progress in the field of creating various functional foods does not stand still and modern products are characterized by the development of various modifications based on them, which allow obtaining nutrients with high energy, biological value, as well as with desired properties. The use of malted barley extract can solve many problems that arise during the heat treatment of raw materials, as a result of the inevitable decrease in its nutritional and biological value. The purpose of the work is to study the effect of micronutrient-rich barley malt extract (malt extract) on the processes of intensifying the production of bread kvass. Obtaining malt extract, analysis of kvass samples was carried out according to generally accepted methods in accordance with the requirements of the standard. The above studies fully explain the relevance and practical significance of the presented studies based on the study of organoleptic and physico-chemical parameters of the finished product. The result of this area of ​​research is the approbation of the replacement of kvass wort 30%; 20% and 10% of the amount of barley malt extract, which allows to intensify the fermentation processes and saturate the product with biologically important micronutrients, and also gives kvass tonic properties, increasing the biological status of the human body. An increase in the concentration of malt extract causes undesirable changes in the organoleptic and physico-chemical parameters of laboratory kvass wort. Reducing the concentration does not affect the biochemistry of raw materials and the use of lower concentrations is not advisable. The results obtained allow us to say that the inclusion of malted barley extract in the composition of the test samples makes it possible to intensify the vital processes of the yeast Sacharomices cerevisiae used in the experiment, which results in a slight increase in the mass fraction of ethyl alcohol, titratable acidity and a decrease in pH in the finished product compared to control. The high content of nutrients, enzymes, vitamins in the composition of SE affects the course of fermentation, intensifying this process, which reduces the fermentation time and increases the profitability of the production of the fermentation product .

Cyclotide-rich fractions containing nanofibers by electrospinning: preparation, characterization and examination of antimicrobial activity.

In this study, antimicrobial nanofibers were produced with the mixtures of polyvinyl alcohol (PVA) and cyclotide-rich fractions by electrospinning method. After extraction, the first separation was carried out with C18 flash chromatography and then fractioned into five separate parts by reversed-phase high-pressure liquid chromatography (RP-HPLC). The molecular weights of cyclotides in each fraction were determined by quadrupole time-of-flight liquid chromatography-mass spectrometry (Q-TOF LC-MS). Cyclotide-rich fractions were mixed with 10% of PVA solution and nanofibers were produced from this biocomposite mixture by electrospinning method. The nanofibers were characterized by field emission scanning electron microscopy (FE-SEM), and it was observed that 100% peptide-containing nanofibers (cyclotide-rich fraction/10% PVA, w/v) had more regular fiber textures. The presence of the peptides in the nanofiber was also confirmed by analytical RP-HPLC, as the peptides in both peptide fractions and nanofiber solutions have the same retention times. The nanofibers produced with the fourth cyclotide-rich fraction showed activity against gram-positive bacteria (Bacillus cereus) in antimicrobial susceptibility test. As a result of these findings, cyclotide-containing nanofibers with antimicrobial activity can be produced for pharmaceutical research and development studies.

К ВОПРОСУ МОНИТОРИНГА ПРОЦЕССА БРОЖЕНИЯ РАЗЛИЧНЫХ ТИПОВ ВИН

A classical approach to monitoring the fer-mentation of grape must based on the use of the areometric method is considered. Based on the processing of the data of measuring the refractive index and density of grape must, as well as data on the physicochemical composi-tion of grape must during fermentation, meth-odological approaches have been developed to create methods for monitoring the composi-tion of the fermenting must using refractomet-ric and refractodensimetric methods of analy-sis. The results of the work can become the basis for the development of a non-destructive express method for determining the volume fraction of ethyl alcohol and the mass concentration of sugars in fermenting must based on the standard equipment of the laboratory of wine-making enterprises, and for the improvement of technical specifications for the manufacture of a portable device for determining the concentration of alcohol and sugar content, based on the simultaneous measuring the refractive index and density of liquid media.

Velocity field and diameter distribution of long-chain alcohol-aviation kerosene transverse jet

The velocity field and diameter distribution of long-chain alcohol-aviation kerosene transverse jets were measured and analyzed. Aviation kerosene was chosen as the base fuel. During the transverse jet process, the droplet velocity direction transformed from the vertical direction to the horizontal direction, i.e., to the crossflow direction. The highest turbulence intensity appeared in the liquid–air interaction field. Both the resultant velocities on XZ and XY planes, Uxz and Uxy, and the component velocities along X, Y and Z coordinates, Ux, Uz, and Uy, increased at first and then decreased with the increment of long-chain alcohol volume proportion while the corresponding proportions are different between that of n-butanol and that of n-pentanol. The existence of vortex region was determined in the liquid–air interaction field and the near-field region in the downstream of the transverse jet. The particle size field analysis showed that the droplet size and shape factor and number density were mainly affected by Weg (Weber number of cross gas flow). The characteristics were different under different breakup modes. With the increase in the volume fraction of long-chain alcohol, the droplet size and Sf (shape factor) first increased and then decreased. The addition of long-chain alcohol also reduced the range of particle size with the largest number density. The maximum size of the droplets in the particle size field of n-pentanol-aviation kerosene was larger than that of n-butanol-aviation kerosene.

Chemical characterization of two fractions from Sanghuangporus sanghuang and evaluation of antidiabetic activity

• SHP and SHEs were firstly obtained from the fermentation broth of S. sanghuang . • SHP and SHEs could significantly promote glucose consumption in vitro . • Although SHP possessed hypoglycemic, it inhibited the cytoactive in vitro . • SHP have remarkably hypoglycemic effect in typeⅡ diabetes model on mice. Sanghuangporus sanghuang polysaccharide (SHP) and ethyl alcohol fractions from the alcohol deposit fluid (SHE) were obtained from the fermentation broth of S. sanghuang . The component analysis by GC–MS showed that 4 organic reagent (petroleum ether, trichloroethane, ethyl acetate and n-butyl alcohol) extractions (SHEs) covered mainly esters, alcohols, phenols, free alkaloids and glycosides. The cytotoxicity and hypoglycemic effect of SHP/SHEs were tested on insulin resistance cell model, the results showed that SHP and SHEs could significantly promote glucose consumption at certain concentrations. Interestingly, SHP not only possessed hypoglycemic, but also inhibited the cytoactive at 2.5–10 mg/mL. Subsequently, the results in type Ⅱ diabetes model on mice revealed that SHP could significantly decrease blood glucose, recover blood lipid levels and repair the damaged tissues at 100–400 mg/Kg·BW. Altogether, our research concluded that SHP possesses antidiabetic activity, suggesting it a prospect application prospect as functional food and medical health product.

Siegesbeckiae Herba Extract and Chlorogenic Acid Ameliorate the Death of HaCaT Keratinocytes Exposed to Airborne Particulate Matter by Mitigating Oxidative Stress.

Airborne particulate matter with a size of 10 μm or less (PM10) can cause oxidative damages and inflammatory reactions in the skin. This study was conducted to discover natural products that are potentially useful in protecting the skin from PM10. Among the hot water extracts of a total of 23 medicinal plants, Siegesbeckiae Herba extract (SHE), which showed the strongest protective effect against PM10 cytotoxicity, was selected, and its mechanism of action and active constituents were explored. SHE ameliorated PM10-induced cell death, lactate dehydrogenase (LDH) release, lipid peroxidation, and reactive oxygen species (ROS) production in HaCaT cells. SHE decreased the expression of KEAP1, a negative regulator of NRF2, and increased the expression of NRF2 target genes, such as HMOX1 and NQO1. SHE selectively induced the enzymes involved in the synthesis of GSH (GCL-c and GCL-m), the regeneration of GSH (GSR and G6PDH), and GSH conjugation of xenobiotics (GSTκ1), rather than the enzymes that directly scavenge ROS (SOD1, CAT, and GPX1). SHE increased the cellular content of GSH and mitigated the oxidation of GSH to GSSG caused by PM10 exposure. Of the solvent fractions of SHE, the n-butyl alcohol (BA) fraction ameliorated cell death in both the absence and presence of PM10. The BA fraction contained a high amount of chlorogenic acid. Chlorogenic acid reduced PM10-induced cell death, LDH release, and ROS production. This study suggests that SHE protects cells from PM10 toxicity by increasing the cellular antioxidant capacity and that chlorogenic acid may be an active phytochemical of SHE.