Food Products Of Animal Origin Research Articles

Chemiluminescence microarray immunoassay for multiple aminoglycoside antibiotics based on carbon nanotube-assisted signal amplification.

The simultaneous determination of multiple analytes has been an urgent demand in screening of antibiotic residues in food products of animal origin due to its higher analysis efficiency. Five aminoglycoside antibiotics (AGAs) have been monitored in milk, including gentamicin (GEN), kanamycin (KAN), neomycin (NEO), and streptomycin/dihydrostreptomycin (STR/diSTR). A chemiluminescence microarray immunoassay (CLMIA) based on nitrocellulose membrane had been developed for the detection of multiple AGAs, which the LODs for STR, KAN, NEO, and GEN were 4.74ng/mL, 4.97ng/mL, 2.99ng/mL, and 4.42ng/mL respectively. To improve the sensitivity of immunoassay, single-well carbon tubes (SWCNTs) were utilized as solid support for loading horseradish peroxidase-labelled goat anti-mouse antibody to obtain the multi-enzyme particles. After the optimization of usage of multi-enzyme particles and antibodies, the enhanced CLMIA was established and evaluated. The LODs were 1.25ng/mL for STR, 0.64ng/mL for KAN, 0.38ng/mL for GEN, and 0.39ng/mL for NEO, which was improved by threefold, sevenfold, 11-fold, and sevenfold compared with the conventional CLMIA developed. These methods presented higher specificity and repeatability. Finally, the enhanced CLMIA based on CNT-assisted multi-enzyme particles was utilized to analyze twenty-five milk samples from local market and dairy farm, which all the results were below the LOD. The enhanced CLMIA showed the great application potential for the detection of multiple targets simultaneously and provided efficient tool for the screening of pollutants in food.

Relevance of food bacterial zoonoses in the Dnipropetrovsk Region (Ukraine) during 2020

When the world is facing an unprecedented COVID-19 pandemic, the importance of animal disease surveillance has become apparent. A significant proportion of new and existing human diseases are zoonoses. Microbiological researches remain relevant today. They guarantee the quality of food raw materials and products, as well as identify sources of infection. The aim of our work was to study the spread of foodborne bacterial zoonoses in the Dnipropetrovsk region during 2020. The study used the methods provided by DSTU ISO 6887-2:2005, DSTU ISO 4833:2006, DSTU ISO 4832:2015, DSTU EN 12824:2004, DSTU ISO 11290-1:2003, DSTU 7444:2013, DSTU ISO 6888-2:2003, DSTU ISO 7937:2006. As a result of the study of the epidemic situation regarding outbreaks of acute intestinal infectious diseases and food poisoning in 2020 in Ukraine, 52 cases were investigated, including cases in which children have been affected (43.8%). In the etiological structure, the largest share of outbreaks caused by salmonella (34.6%) and outbreaks of viral etiology (25%) was observed. The registration of outbreaks of human salmonellosis in 82% of regions in Ukraine proves that geographical conditionality, climatic conditions, species structure of farm animals and the level of socio-economic status of the population are important factors in the development of the disease. The presence of positive for animal salmonellosis localities also demonstrates the constant spread of this zoonosis in Ukraine, which causes contamination by these bacteria of food products of animal origin and environmental objects. The dominance of the Dnipropetrovsk Region in 2020 in the number of outbreaks of acute intestinal infectious diseases contributed to a detailed investigation of bacteriological studies of raw materials of animal origin and food products, which revealed 2.56% of non-compliant samples, where the highest percentage of violations of safety criteria was poultry products. Thus, high-risk products included semi-finished products and culinary products from meat, in particular poultry; minced meat and mechanically rolled meat, as well as ready-to-eat meat products — sausages, namely poultry, and eggs. Poultry products of seven types (n = 3,226) were analyzed for the presence of sanitary-indicative microflora (coliform bacteria, mesophilic aerobic and facultative-anaerobic microorganisms), opportunistic microflora (coagulase-positive staphylococci, Proteus, sulfite-reducing clostridia), and pathogenic microflora (bacteria of the genera Salmonella, Listeria). The percentage of samples that did not meet the requirements of regulatory documentation by indicators was: mesophilic aerobic and facultative anaerobic microorganisms up to 10.92%; coliform bacteria — up to 3.90%. Pathogenic contaminants of poultry products of domestic Ukrainian production were: Salmonella Enteritidis (up to 4.20%), Listeria monocytogenes — 12.50%. The paper shows problematic in 2020 nosological forms in the structure of pathogens of food bacterial zoonoses and identifies their potential sources. Microbiological studies have shown that the contaminants of poultry products of domestic Ukrainian production in the Dnipropetrovsk Region were sanitary-indicative (coliform bacteria, mesophilic aerobic and facultative-anaerobic microorganisms), opportunistic pathogens (Staphylococcus aureus, sulfite-reducing clostridia), and pathogenic microflora (bacteria of the genera Salmonella, Listeria). The author has monitored the geographical serotypic predisposition of Salmonella, which is due to the prevalence of O-group D in the Dnipropetrovsk Region, and the possibility of their passage through the food chain. It was found that minced meat and meat of mechanical deboning from poultry is one of the factors of transmission of a potentially pathogenic for humans and animals species of Listeria — L. monocytogenes in the region

Scenario forecasting of consumption of main organic livestock products in Ukraine

Purpose. The aim of the article is to develop scenarios of consumption of the main types of organic livestock products in Ukraine. These scenarios should include the creation of an effective institutional environment aimed not only at strict quality control, but also at stimulating the modernization of production, the implementation of environmentally friendly technologies and genetic technologies for the production of safe products.
 Methodology / approach. The methodological basis of the study is an integrated (systemic) approach, which allows forecasting more accurately the reproduction of the processes of quality supply of organic livestock products, taking into account the factors that affect it. Methodological aspects of calculations in forecasting the capacity of new segments of the organic market (which replace the market segments of traditional or mass products) are based on criteria of rational consumption, taking into account the influence of such factors: potential of organic livestock production in Ukraine, number of households (consumers), income of households (consumers), differentiation of household incomes (consumers), prices for organic livestock products, prices for livestock products originating from the traditional (mass) sector, consumer confidence in the quality assurance system for livestock products (including state quality control systems), consumer confidence in the organic certification system of livestock products. 
 Results. The main factors influencing the consumption of organic livestock products in Ukraine are identified, among which the main ones are the price and quality of products. The reasons of low rates of production of livestock products development with protected geographical names and organic products in Ukraine are described. In particular, it is substantiated that due to the high cost, complexity of production technologies, high cost of certification, the long transition period to organic production, the production of organic livestock products does not reach potential. 
 Originality / scientific novelty. The forecasting of the capacity of the organic sector is made under the conditions of partial or complex implementation of measures aimed at improving the quality of livestock products with the definition of inertial, innovative and systemic scenarios. The authors identified the system scenario as a priority. According to the systemic variant in Ukraine for the period up to 2025 the forecasted capacity of the internal market for organic meat and meat products (in terms of meat) will amount to 3.26 bln UAH; organic milk, dairy products and butter (in terms of milk) will amount to 2.65 bln UAH; organic eggs will amount to 0.47 bln UAH. In general, the market potential capacity of the main types of organic livestock products (at prices in 2019) will be 6.38 bln UAH (0.2 bln Euro) or 151.9 UAH (4.9 Euro) per one person. This is much less than the current European figure of 50 euros per person. In the potential consumer market, the share of organic livestock products in accordance with the systemic version of the solution of the quality problem will be from 1.6 % (meat and meat products) and 1.5 % (milk and dairy products) to 2.3 % (eggs), which is much lower than European indicators.
 Practical value / implications. Scenario forecasting of the dynamics of potential demand for organic livestock products allows understanding the pace of development of the domestic market of organic products, justification of measures and tools to stimulate domestic organic production, determining the impact of these measures on the capacity of the organic market. Otherwise, a significant market share of organic livestock products and food products of animal origin, including milk and dairy products, will be filled by imported products of European producers. The authors identified as a priority systemic scenario for the development of consumption of the main types of organic livestock products in Ukraine.

Antibacterial activity of clove (Syzygium aromaticum) and cinnamon (Cinnamomum burmannii) essential oil against extended-spectrum β-lactamase-producing bacteria.

Background and Aim:Extended-spectrum β-lactamase (ESBL) is an enzyme produced by the family of Enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae, which can hydrolyzeβ-lactam antibiotics, such as penicillins, cephalosporins, cephamycin, and carbapenem. ESBL-producing bacteria are widely distributed from farms to slaughterhouses until food products originating from animals are available in the market, which plays an important role as a pathway for the exposure and transmission of ESBL-producing bacteria from food products of animal origin to humans. This study aimed to determine the antibacterial activity of Syzygium aromaticum (clove) and Cinnamomum verum (cinnamon) essential oils against strains resistant to ESBL-producing E. coli and K. pneumoniae isolates.Materials and Methods:The antibacterial activity of clove and cinnamon essential oils was tested against three strains of tested bacteria using the disk diffusion method. The minimum inhibitory concentration (MIC) of clove and cinnamon essential oils was determined using the broth microdilution method. The minimumbactericidal concentration (MBC) was determined using the MIC. Morphological changes on each tested bacteria were observed through scanning electron microscopy (SEM).Results:Both essential oils exhibited inhibitory effects toward all test organisms, indicated by inhibition zones around the disk. The MIC values of clove essential oil were 0.078% (v/v) for all tested bacteria, whereas the MICs of cinnamon essential oil ranged from 0.039% (v/v) to 0.156% (v/v) for all tested bacteria. MBC values of clove and cinnamon essential oils ranged from 0.078% (v/v) to 0.156% (v/v) for all tested bacteria. There were morphological changes in each tested bacterial cell that was observed through SEM. Each tested bacteria treated with clove and cinnamon essential oils showed shrinkage and cells lysis.Conclusion:It was concluded that clove and cinnamon essential oils have emerged as effective antibacterial agents by showing high antibacterial activity against ESBL-producing E. coli and K. pneumoniae isolates, as evidenced by the inhibition zone diameter and MIC value.

Prevalence and Antimicrobial Resistance Profile of Different Salmonella serovars Isolated from Food Products of Animal Origin

Salmonella is the major cause of foodborne diseases and a serious public health problem in the world, with an increasing concern for the emergence and spread of antimicrobial-resistant strains. Our study was conducted to assess the prevalence and antimicrobial resistance profiles of Salmonella isolates using standard bacteriological methods. The overall prevalence rate of 11.4% was recorded from the total analyzed food items of animal origin. Salmonella isolates were detected from 5.7% of minced meat, 1.4% of kofta, 1.4% of luncheon, and 2.8% of burger. All Salmonella species recovered were resistant to amoxicillin-clavulanic acid with 100% sensitivity to ciprofloxacin and meropenem. Findings on the multidrug-resistant (MDR) profile showed that a total of 6/8 (75%) of Salmonella Enteritidis were resistant to 3 or more antibiotics. Therefore, our findings provide the prevalence and drug resistance of Salmonella from foods of animal origin and contribute information to scientists as well as public health researchers to minimize the prevalent and resistant foodborne Salmonella species in Egypt.

Emergence and Clonal Spread of CTX-M-65-Producing Escherichia coli From Retail Meat in Portugal.

The emergence and dissemination of resistance to third- and fourth-generation cephalosporins among Enterobacteriaceae from different sources impose a global public health threat. Here, we characterized by whole-genome sequencing four Escherichia coli strains harboring the blaCTX–M–65 gene identified among 49 isolates from beef and pork collected at retail. The genomic content was determined using the Center for Genomic Epidemiology web tools. Additionally, the prediction and reconstruction of plasmids were conducted, the genetic platform of the blaCTX–M–65 genes was investigated, and phylogenetic analysis was carried out using 17 other genomes with the same sequence type and harboring the blaCTX–M–65 gene. All strains harbored blaCTX–M–65, blaOXA–1, and blaTEM–1B, and one also carried the blaSHV–12 gene. Other resistance genes, namely, qnrS2, aac(6′)-Ib-c, dfrA14, sul2, tetA, and mphA, were present in all the genomes; the mcr-1.1 gene was identified in the colistin-resistant strains. They belong to sequence type 2179, phylogenetic group B1, and serotype O9:H9 and carried plasmids IncI, IncFIC(FII), and IncFIB. All strains share an identical genetic environment with IS903 and ISEcp1 flanking the blaCTX–M–65 gene. It seems likely that the blaCTX–M–65 gene is located in the chromosome in all isolates based on deep in silico analysis. Our findings showed that the strains are clonally related and belong to two sub-lineages. This study reports the emergence of CTX-M-65-producing E. coli in Portugal in food products of animal origin. The chromosomal location of the blaCTX–M–65 gene may ensure a stable spread of resistance in the absence of selective pressure.

Determination of quinoxalines veterinary drug residues in food products of animal origin by high performance liquid chromatography-tandem mass spectrometry

To establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for simultaneous determination of olaquindox, quinoxaline-2-carboxylic acid, 3-methyl-quinoxaline-2-carboxylic acid in food products of animal origin. Samples were extracted with ethyl acetate-0.1 mol/L sodium dihydrogen phosphate(1∶9, V/V) solution in water bath at 40 ℃, purified by solid phase extraction column(olaquindox uses HLB SPE cartridge, quinoxaline-2-carboxylic acid and 3-methyl-quinoxaline-2-carboxylic acid use MAX SPE cartridge), evaporated by concentrator, and dissolved by mobile phase. The supernatant was separated on Agilent ZORBAX SB-C_(18) column(2.1 mm×50 mm, 1.8 m), using 0.2% formic acid solution methanol solution as mobile phase, and then detected by HPLC-MS/MS using multiple reaction monitoring(MRM) in positive ionization mode. The linear range for olaquindox、quinoxaline-2-carboxylic acid and 3-methyl-quinoxaline-2-carboxylic acid were 2.5-100 μg/L, r & gt; 0.9990.The average recoveries were 72.6%-90.5% and the precisions were 3.2%-13.1%(n=6). The detection limits for olaquindox、quinoxaline-2-carboxylic acid and 3-methyl-quinoxaline-2-carboxylic acid were 0.08 μg/kg. The method is specific, sensitive, easy, fast and suitable for the confirmation and quantification of olaquindox、quinoxaline-2-carboxylic acid、3-methyl-quinoxaline-2-carboxylic acid in food products of animal origin.

Pathogenic Organisms in Varanidae and Their Potential as Zoonotic Diseases

<p class="00-6Abstrak2Wtz">Monitor lizards have various body sizes, belong to the Varanidae and it is reported that there were various kinds of pathogenic organisms in their bodies. This review aims to describe various information on pathogenic organisms and their potential as zoonotic diseases and can be used as a reference in the prevention of zoonotic diseases originating from Varanidae. The results of this review discuss two types of viruses, three types of bacteria, five types of protozoa, two types of arthropods, and seven types of endoparasites that are pathogenic in Varanidae. Based on this information it is known that potentially zoonotic pathogens include bacteria group is <em>Salmonella </em>spp. and <em>Anaplasma phagocytophilum</em>; groups of protozoa was <em>Giardia </em>sp<em>p</em>. and <em>Cryptosporidium </em>sp.; Arthropod group namely <em>Amblyomma </em>sp<em>.</em> which carries the bacteria <em>Rickettsia </em>sp<em>.</em>, <em>Borrelia </em>spp<em>.</em> and <em>Anaplasma </em>spp<em>.</em> and the endoparasite group consisting of <em>Ascaris </em>sp<em>.</em>, <em>Strongyloides </em>sp<em>.</em>, Pentastomida, and <em>Angiostrongylus cantonensis</em>, while for the virus group, there were no pathogenic organisms with zoonotic potential. The extensive use of Varanidae by humans as exotic pets, as well as for consumption and medicine has great potential in the transmission of zoonotic diseases. Those are possible because the varanids non-aseptically environment. The proper handling and maintenance of monitor lizards are necessary to prevent the spread of zoonotic diseases. Unhygienic and less aseptic way of handling food products of animal origin can lead to the potential for transmission of pathogenic organisms (foodborne disease). Efforts to prevent the transmission of foodborne diseases can apply Good Hygienic Practices (GHP) with the process of cooking reptile meat for consumption. Besides, disease transmission can be prevented by implementing the One Health concept.</p>

Dynamics of poultry and the current state of poultry in the Western region of Ukraine

One of the most attractive and promising types of agrarian business in Ukraine is poultry farming, the rapid development of which ensures high competitiveness, due to the short period of production, lower compared to other food products of animal origin, price and high demand among consumers. Now, the market of our country has significantly increased demand for turkey meat, which has practically no contraindications, as it does not contain cholesterol, is a gentle, hypoallergenic, dietary product that is rich in unsaturated fatty acids, including omega-3, proteins, essential amino acids, minerals, vitamins A and B, etc. The purpose of the work was to investigate the dynamics of poultry in the period from 2011 to 2020 and to get the current state of poultry in general, and turkey farming in particular, in the Western region of Ukraine. It is found that in the period from 2011 to 2020 the largest number of poultry, 9548.3 ± 447.1 thousand, was kept in farms of Lviv region, slightly less – 8109.1 ± 546,1 thousand of Khmelnytsk region, even less – 7222.1 ± 121.0 and 7682.9 ± 316.2 thousand Volynj and Rivne regions. The farms of Ivano-Frankivsk, Ternopilj, Transcarpathian and Chernivtsi regions was kept 5290.8 ± 284.7 5200.2 ± 86.2, 4127.6 ± 115.2 and 3636.7 ± 50.5 thousand poultry, respectively. The largest poultry population in the farms of Lviv and Volynj regions was in 2020 and amounted to 11938,1 and 8822.4 thousand, respectively, Rivne region – 7742.3 thousand in 2018, Transcarpathian – 4385.8 thousand in 2015, Chernivtsi – 3863.9 thousand in 2012, Ternopilj – 5584.2 thousand in 2019, Khmelnytskyj – 10864.5 thousand in 2014 and Ivano-Frankivsk – 6761.1 thousand in 2011.The lowest number of poultry in farms of Lviv, Rivne, Volynj, Transcarpathian, Ternopilj, Khmelnytskyj regions was in 2011, the number of which was 7961.5, 6721.2, 5933.7, 3126.1, 4681.6, 5069.7 thousand, respectively, and Chernivtsi and Ivano-Frankivsk – 3376.5 and 4175.7 thousand in 2016 and 2017. The smallest number of turkey farms in Western Ukraine was in the period from 2011 to 2013. By 2017, there was an increase in their number by 20 %, and in 2018 – a decrease of 10 % compared to 2017. In 2019, the number of registered enterprises increased by 18.18 %, compared to 2018, and in 2020 their number was the largest.

Novel Sequence Types of Listeria monocytogenes of Different Origin Obtained in the Republic of Serbia.

Listeria monocytogenes, the causative agent of listeriosis, is amongst the major food-borne pathogens in the world that affect mammal species, including humans. This microorganism has been associated with both sporadic episodes and large outbreaks of human listeriosis worldwide, with high mortality rates. In this study, the main sequence types (STs) and clonal complexes (CCs) were investigated in all of the 13 L. monocytogenes strains originating from different sources in the Republic of Serbia in 2004–2019 and that were available in the BIGSdb-Lm database. We found at least 13 STs belonging to the phylogenetic lineages I and II. These strains were represented by ST1/ST3/ST9 of CC1/CC3/CC9, which were common in the majority of the European countries and worldwide, as well as by eight novel STs (ST1232/ST1233/ST1234/ST1235/ST1238/ST1236/ST1237/ST1242) of CC19/CC155/CC5/CC21/CC3/CC315/CC37, and the rare ST32 (clonal complex ST32) and ST734 (CC1), reported in the Republic of Serbia, the EU, for the first time. Our study confirmed the association of CC1 with cases of neuroinfection and abortions among small ruminants, and of CC3 and CC9 with food products of animal origin. The strains isolated in 2019 carried alleles of the internalin genes (inlA/inlB/inlC/inlE) characteristic of the most virulent strains from the hypervirulent CC1. These findings demonstrated the genetic relatedness between L. monocytogenes strains isolated in the Republic of Serbia and worldwide. Our study adds further information about the diversity of the L. monocytogenes genotypes of small ruminants and food products, as the strain distribution in these sources in Serbia had not previously been evaluated.

Assessment of various veterinary drug residues in animal originated food products.

The veterinary drugs are broad-spectrum antibacterial antibiotics; it uses to cure the animal disease. Many countries have banned veterinary drug residues like nitrofurans metabolites, chloramphenicol. However, the people were administrated veterinary drugs to animals as illegal to increase the milk production in animals for economic benefit. The results of illegally use of veterinary drugs remain as a residue in animal product like milk and it is very harmful to whom consume it cause cancer and allergic for human being which has entered the concern among milk consumers. To control illegal use of veterinary drugs, the government of India has restricted its use in animals. For the identification and confirmation of veterinary drug residues in animal products, analytical techniques such as liquid chromatography and mass spectrometry are available. These are very sophisticated equipments which are available nowadays and their methodologies for the analytical method validation are described by European commission 2002/657/EC. The use of veterinary drugs is a big challenge to effectively identify and authorization of their use. There are so many analytical techniques are using very effectively and taking very less time to protect the consumers from their adverse effects. These techniques take very less time to identify more groups of compounds such as tetracycline, sulfonamides, anthelmintic, and macrolides in single multi-residue method. These methods having validation parameters include system precision, calibration curve, accuracy, limit of detection, and quantification. Therefore, improvement in the existing technologies and accessibility of new screening methodologies will give opportunities for automation that helps in obtaining the results in very less time and improved sensitivity and specificity which contribute to better safety assurance, standard, and quality of various food products of animal origin.

Молекулярная гастрономия – стимул для инновационных исследований различных аспектов, связанных с процессом питания

The growing competition in the market of food manufacturers in recent years makes more and more demands on the quality and consumer appeal of food products. The necessity of continuous improvement of the senso-ry properties of products determines high requirements for the professional knowledge of industrial technol-ogists. Today, there is no doubt that the food industry is experiencing a significant shortage of specialists who have systemic and commercially applicable knowledge of both the organoleptic analysis itself and the mechanisms of molecular transformations and processes that affect the formation of sensory properties of food.
 The modern scientific discipline "Molecular gastronomy" has significant potential for systematizing knowledge regarding mechanisms of chemical and physical changes that occur in a product during techno-logical processing, and their influence on the formation of organoleptic properties of products. We believe that the inclusion of this discipline in the educational process to study specialists of the directions "Food products of animal origin" and "Food products from vegetable raw materials" is highly-demanded and well-timed. In the fishing industry, the studying of technologists for this discipline can be implemented within the profiles ''Technology of products from aquatic biological resources'' and ''Food biotechnology of aquatic or-ganisms''. This discipline can be included both in the preparation of bachelors within these profiles, and in the educational program for the preparation of masters.

Contribution of Nanomaterials to the Development of Electrochemical Aptasensors for the Detection of Antimicrobial Residues in Food Products

The detection of antimicrobial residues in food products of animal origin is of utmost importance. Indeed antimicrobial residues could be present in animal derived food products because of animal treatments for curative purposes or from illegal use. The usual screening methods to detect antimicrobial residues in food are microbiological, immunological or physico-chemical methods. The development of biosensors to propose sensitive, cheap and quick alternatives to classical methods is constantly increasing. Aptasensors are one of the major trends proposed in the literature, in parallel with the development of immunosensors based on antibodies. The characteristics of electrochemical sensors (i.e., low cost, miniaturization, and portable instrumentation) make them very good candidates to develop screening methods for antimicrobial residues in food products. This review will focus on the recent advances in the development of electrochemical aptasensors for the detection of antimicrobial residues in food products. The contribution of nanomaterials to improve the performance characteristics of electrochemical aptasensors (e.g., Sensitivity, easiness, stability) in the last ten years, as well as signal amplification techniques will be highlighted.

Preparation of Food Products of Animal Origin with a Reduced Content of Toxic Metals

Excessive concentrations of heavy metals in food we eat are among the current issues of food safety. This becomes especially evident if we consider highly toxic elements such as copper, lead and cadmium. With regard to the foregoing, it would be helpful to get information about use of novel sorbents in livestock farming and their effects on distribution of heavy metals in animal bodies. The drug we study is based on natural mineral raw materials and contains up to 60% of silicon dioxide. It has prominent sorption properties. The drug was introduced to diets of brood sows, growing piglets and finishing pigs in a dose 120 mg/kg of body mass. Lead concentrations in three-month-old piglets decreased by 17% in striated muscle tissue; and in the skin and hair covering of the piglets receiving sorbents there were just traces of lead. In striated muscle tissue of piglets in the control group the cadmium levels were 0.14±0.003 mg/kg, i.e. 2.8 times higher than the maximum permissible concentration. Sorbents introduces to the piglet diet caused decrease in concentrations of this metal by 35%. Use of the sorptive dietary supplement has certain prospects as a method of obtaining organic food.

METHODS OF INDICATION OF E. COLI AND E. COLI GROUP BACTERIA IN MEAT

The article provides an overview of methods for detecting bacteria of the Escherichia coli group, including E. coli. This group of microorganisms is one of the main standardized indicators when assessing the quality of food products of animal origin. Analysis of microbiological studies of chilled beef meat samples showed that when determining the presence of BGKP in samples, using traditional and modern methods of analysis, the results do not differ significantly. For the differentiation of enterobacteria, Endo's medium and a selective agar chromogenic medium Chromocult Coliform Agar (Merck, Germany) were used. The amount of BGKP in 1 cm3 of meat products varied from 0.3 × 102 CFU / cm3 to 1.6 × 102-1.8 × 102 CFU / cm3. When microcopying smears in all samples, small rods with rounded ends were found, according to Gram they were negatively stained. When growing on Endo agar, colonies of red color with a metallic sheen were noted; on a chromogenic medium, the growth of violet colonies was noted. During the tests, microorganisms were identified from the samples, representatives of the genera: Escherichia, Enterobacter, Citrobacter. The use of modern methods of analysis made it possible to reduce the labor costs that were spent on the preparation of media, and also made it possible to exclude the stages of confirmatory biochemical tests, thanks to the use of culture media with markers of specific enzymatic activity.

Technology and commodity characteristics of extracts and oil cake for food enrichment

The original technology of obtaining a new class of food additives in the form of CO2 extracts has been developed at the department «Technology of food products of animal origin» in Kuban State Technological University. It was found that after removal of CO2–extractive substances from dry raw material, sterile oil cake is formed, which is a carbohydrate-protein-lipid food additive. One more peculiarity of CO2 oil cake was noted: the pressure in the extraction apparatus suddenly drops from 6 MPa to atmospheric pressure at the end of the extraction process, the solvent located in each particle of raw material boils up sharply, and at the same time, a particle of raw materials is being reground to a nanoscale. If the microorganisms are in the raw materials they are subjected to the same process. The oil cake becomes infection-free after a gas-liquid bursting. The physicochemical properties of CO2 extracts from the dried fruits of actinidia, sea buckthorn, unabi and rosehips were investigated. The extract form, density and refractive index, viscosity and acid number are determined by these characteristics. The oil cake obtained after the removal of CO2-extractive substances from the raw materials was analysed for their content of the main food components, macro and microelements. The formulation of a meat and vegetable paste enriched with CO2 extracts and CO2 oil cake has been developed.

Antimicrobial uses for livestock production in developing countries.

Antimicrobial is an indispensable part of veterinary medicine used for the treatment and control of diseases as well as a growth promoter in livestock production. Frequent use of antimicrobials in veterinary practices may lead to the residue in animal originated products and creates some potential problems for human health. The presence of antimicrobial residues in animal originated foods may induce serious health problems such as allergic reaction, antimicrobial resistance (AMR), and lead to carcinogenic and mutagenic effects in the human body. The misuse or abuse of antibiotics in human medicine is thought to be a principal cause of AMR but some antimicrobial-resistant bacteria and their resistant genes originating from animals are also responsible for developing AMR. However, the residual effect of antimicrobials in feed and food products of animal origin is undeniable. In developing countries, the community is unaware of this residual effect due to lack of proper information about antibiotic usage, AMR surveillance, and residue monitoring system. It is imperative to reveal the current situation of antimicrobial use in livestock production and its impacts on public health. Moreover, the safety levels of animal feeds and food products of animal origin must be strictly monitored and public awareness should be developed against the indiscriminate use of antimicrobial in animal production. Therefore, the current review summarizes the literature on antimicrobial use in livestock production and its hazardous residual impacts on the human body in developing countries.

The use of antibiotics in food technology: the case study of products from Moscow stores

The global threat of the 21st century is the uncontrolled use of antibiotics. Many bacteria have developed resistance to antibacterial drugs, resulting in drug-resistant superbugs. The article presents the results of the case study of Russian food products from the stores in the city of Moscow and analyzes them for the presence of antibiotics. The object of the study is the Russian market of animal products. The subject of the study is detecting the presence of antibiotics in food products on the Russian market. As a result of studying of the range of food products of animal origin, it has been revealed that the amount of harmful substances (such as antibiotics) meets the MPC standards, according to the Russian GOST standards. The research results obtained in laboratory conditions are of interest for assessing the prevalence of antibiotic residues in meat and dairy products. Based on the monitoring studies, it has been found out that most of the residual antibiotics were present in poultry meat. In all three samples, the antibiotic enrofloxacin was found but the MPC of the antibiotic was not exceeded (the norm is not more than 100 μg / kg).

Food container employing a cold atmospheric plasma source for prolonged preservation of plant and animal origin food products

Cold Atmospheric Plasma is a non-thermal processing technology with great potential for application to food products as it can effectively reduce the microbial load, leading to substantial shelf-life extension. Herein, we present an easy-to-build and cost-effective Surface Dielectric Barrier Discharge (SDBD) plasma source adjusted to the plastic lid of a common commercial food container made of transparent glass. Implementation and evaluation of plasma treatment in real perishable food products such as sea bream fillets, fresh-cut rocket salads and fresh whole strawberries showed that such device might be efficiently used in-storage for the extension of their shelf-life.•Easy-to-build and cost-effective SDBD plasma source adjusted in a food container for generation of antimicrobial RONS in proximity to treated food product•Treatment of perishable food products by reducing their initial microbial load•In-storage treatment efficient for perishable food products shelf-life extension

Technological Applications of Macrococcus caseolyticus and its Impact on Food Safety.

Macrococcus spp. are Gram-positive cocci that belong to the Staphylococcaceae family; they are closely related to staphylococci, but, unlike staphylococci, they are not considered as human pathogens. Macrococcus spp. are recognized as relevant veterinary pathogens, and their presence has been reported in food products of animal origin. Macrococcus caseolyticus, the most studied species of the Macrococcus genus, is associated with the development of aroma and flavor in fermented foods and is, thus, used as starter cultures in fermentations. However, certain important issues regarding food safety must be taken into account when employing these microorganisms in fermentations. Recent studies have reported the presence of genes associated with resistance to methicillin and other antibiotics in M. caseolyticus. This can be harmful to human health as these genes can be transferred to other bacteria present in the food, mainly staphylococcal species. This work, therefore, aims to highlight the importance of a more critical view on the presence of macrococci in foods and the possible indirect risks to human health.