Foam Scaffolds Research Articles

Three-dimensional flexible and stretchable gold foam scaffold for real-time electrochemical sensing in cells and in vivo

Compared with typical two-dimensional electrodes, the three-dimensional (3D) cell culture platform can simulate the real cell survival environment for cell growth to accurately reproduce cell functions. Moreover, considering that living cells are exposed to various of mechanical force in the microenvironment, the construction of 3D electrodes with excellent flexible, stretchable, and biocompatibility is of great significance to real-time monitor mechanically evoked biomolecule release from cells. Herein, we demonstrated a straightforward and effective three-step approach to fabricate three-dimensional flexible and stretchable gold foam scaffold (3D Au foam scaffold) for construction of 3D cell culture integrated electrochemical sensing platform. The excellent biological and electrical properties of Au nanostructures and porous networks of the 3D scaffold endow the platform with desirable biocompatibility and sensitive electrochemical sensing performance. As a proof of concept, the 3D Au foam scaffold functionalized with cobalt based nanocubes (Co NCs/Au foam scaffold) was validated to provide 3D culture for human umbilical vein endothelial cells (HUVECs), and synchronously real-time monitor superoxide anion (O2•-) released by HUVECs under mechanical stretching. Furthermore, 3-mercaptopropionic acid (3-MPA) modified 3D Au foam (3-MPA/Au foam scaffold) was successfully used for real-time monitoring of catecholamines in rat brain. The results demonstrate the great potential of this 3D Au foam scaffold for real-time electrochemical monitoring biomolecules in vitro and in vivo, providing convenience for future research on mechanotransduction relevant processes.

Construction of a 3D interconnected boron nitride nanosheets in a PDMS matrix for high thermal conductivity and high deformability

With the electronic device advancing to miniaturization, higher integration and flexibility, flexible polymer composites with high thermal conductivity are desirable for efficient removal of accumulated heat to maintain normal operation of electronics. In this work, a polydimethylsiloxane/boron nitride nanosheets (PDMS/BNNS) foam scaffold was prepared by the sugar-templated method, and the corresponding PDMS/BNNS composites were manufactured with vacuum-assisted PDMS impregnation and curing. The PDMS/BNNS composites exhibit a three-dimensional (3D) BNNS interconnected network with curved BNNS pathways due to the intensive compression during hot-pressing curing, providing thermally conductive network and corresponding prestrains for deformable application. The PDMS/BNNS composites finally can achieve a high thermal conductivity of 7.55 W m−1 K−1 in the in-plane direction and 1.12 W m−1 K−1 in the through-plane direction with 25 vol% BNNS, which represent 153% and 78% increases over the composites prepared by randomly mixing method, respectively. In addition, the composite still maintains superior heat dissipation property under repeated stretching and bending conditions, which indicates a broad and bright application for thermal management in flexible electronic devices.

Fabrication of ceramic bioscaffolds from fly ash cenosphere by susceptor-assisted microwave sintering

Cenospheres (CS) are ceramic hollow microspheres and have been used to prepare composite foams for applications such as medical implants. However, its potential standalone application in the biomedical field is not fully explored. Herein, a susceptor-assisted microwave (SMW) sintering approach was used for producing CS foam scaffolds. Owing to the hybrid heating mechanism offered by the SMW process, sintering of the low-dielectric cenospheres was realized. We found that sintering was initiated at a lower temperature (1100 °C) compared to conventional heating (1250 °C) as reported in the literature, probably due to the lower activation energy required by SMW sintering. The physical and compositional properties of the sintered CS specimens were examined, and in vitro studies were performed. The as-fabricated CS foam possessed minimal effect on cell viability. Cells migrated and adhered well within the pores of the specimens, which indicates the potential of the CS as scaffold materials for cell engineering applications.

Culture and in situ H2O2-mediated electrochemical study of cancer cells using three-dimensional scaffold based on graphene foam coated with Fe3O4 nanozyme.

For real-time evaluation of the cell behavior and function under in vivo-like 3D environment, the 3D functionalized scaffolds simultaneously integrate the function of 3D cell culture, and electrochemical sensing is a convincing candidate. Herein, Fe3O4 nanoparticles as the nanozyme (peroxide oxidase mimics) were modified on graphene foam scaffold to construct a 3D integrated platform. The platform displayed a wide linear range of 100nM to 20μM and a high sensitivity of 53.2 nA μM-1 toward detection of hydrogen peroxide (H2O2) under the working potential of + 0.6V (vs. Ag/AgCl). The obtained 3D scaffold also displayed satisfactory selectivity toward the possible interferents that appeared in the cell culture environment. Furthermore, the cells still maintained high cell viability (almost 100%) after their growth and proliferation on the scaffold for 7days. With the superior performance on cell culture and electrochemical monitoring, the functions on the 3D culture of MCF-7 or HeLa cells and in situ monitoring of cell-released H2O2 was easily achieved on this 3D platform, which show its great application prospects on further cancer-related disease diagnosis or drug screening. A nanozyme-based three-dimensional graphene scaffold was successfully constructed for cell culture and identification of cancer cells through in situ electrochemical monitoring of the cell-released H2O2.

Hierarchical Oα-rich Co3O4 nanoarray anchored on Ni foam with superior lithiophilicity enabling ultrastable lithium metal batteries

Lithium metal batteries (LMBs) are considered as the ultimate choice in the next-generation high performance energy-storage systems due to their outstanding theoretical energy density (≥500 Wh kg−1). However, the unavoidable dendrite issues and infinite volume change during repeated plating/stripping induce poor electrochemical performance and serious safety issues. Here, we designed and prepared an integrated Oα (O- or O22–)-rich Co3O4 nanoarrays anchored on Ni foam (Oα-Co3O4@NF) scaffold as a stable host for ultra-fast lithium metal infusion. Remarkably, the highly reactive Oα behaves low energy bonding and strong electron affinity, which are further verified by the results of density functional theory, giving rise to high lithiophilicity and inhibiting the dendrites formation effectively. Moreover, the by-product NiO formed on the NF during the calcination process combines with Oα-Co3O4 to display superior dual-wettability toward molten Li. As a result, the Oα-Co3O4@NF electrode achieves a Coulombic efficiency above 99.00% more than 450 cycles at a current density of 1 mA cm−2, and the Oα-Co3O4@NF-Li anode presents a super-long and stable lifetime of 800 h during the repeated plating/striping process. When coupled with a high-loading LiFePO4 cathode, the full cells deliver excellent rate capability and 88.96% capacity retention after 200 cycles under 0.5C.

Three-Dimensional Flexible and Stretchable Gold Foam Scaffold for Real-Time Electrochemical Sensing in Cells and in Vivo

Three-Dimensional Flexible and Stretchable Gold Foam Scaffold for Real-Time Electrochemical Sensing in Cells and in Vivo

Three Component Composite Scaffolds Based on PCL, Hydroxyapatite, and L-Lysine Obtained in TIPS-SL: Bioactive Material for Bone Tissue Engineering

In this research, we describe the properties of three-component composite foam scaffolds based on poly(ε-caprolactone) (PCL) as a matrix and hydroxyapatite whiskers (HAP) and L-Lysine as fillers (PCL/HAP/Lys with wt% ratio 50/48/2). The scaffolds were prepared using a thermally induced phase separation technique supported by salt leaching (TIPS-SL). All materials were precisely characterized: porosity, density, water uptake, wettability, DSC, and TGA measurements and compression tests were carried out. The microstructure of the obtained scaffolds was analyzed via SEM. It was found that the PCL/HAP/Lys scaffold has a 45% higher Young’s modulus and better wettability compared to the PCL/HAP system. At the same time, the porosity of the system was ~90%. The osteoblast hFOB 1.19 cell response was also investigated in osteogenic conditions (39 °C) and the cytokine release profile of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was determined. Modification of PCL scaffolds with HAP and L-Lysine significantly improved the proliferation of pre-osteoblasts cultured on such materials.

A poly (saccharide-ester-urethane) scaffold for mammalian cell growth.

Chitosan and poly(3-hydroxybutyrate) are non-toxic, biodegradable, and biocompatible polymers extensively used in regenerative medicine. However, it is unknown whether the chemical combination of these polymers can produce a biomaterial that induces an appropriate cellular response in vitro in mammalian cells. This study aimed to test the ability of a novel salt-leached polyurethane scaffold of chitosan grafted with poly(3-hydroxybutyrate) to support the growth of three mammalian cell lines of different origin: a) HEK-293 cells, b) i28 mouse myoblasts, and c) human dermal fibroblasts. The viability of the cells was assessed by either evaluation of their capacity to maintain the expression of the green fluorescent protein by adenoviral transduction or by esterase activity and plasma membrane integrity. The results indicated that the three cell lines attached well to the scaffold; however, when i28 cells were induced to differentiate, they did not produce morphologically distinct myofibers, and cell growth ceased. In conclusion, the findings reveal that, altogether, these observations suggest that this foam scaffold supports cell growth and proliferation but may not apply to all cell types. Hence, one crucial question yet to be resolved is a poly (saccharide-ester-urethane) derivative with a nano-topography that elicits a similar cellular response for different biological environments.

Fully Reduced HMGB1-Containing Peptide-Based Polyurethane Scaffold with Minimal Functional Unit of Skin (MFUS) Enhances Large and Deep Wounded Skin Healing.

A novel peptide-based polymer is developed by lysine-diisocyanate (LDI), glycerol (Gly), and fully reduced HMGB1 (frHMGB1). This frHMGB1-LDI-Gly polymer either forms sponge-like foam (scaffold) or a hydrogel or a film under different reaction conditions. It degrades into nontoxic lysine, glycerol, and frHMGB1. The hydrogel glues tissues together and the glued tissues have strong mechanical properties. The film and scaffold provide the suitable environment for enhancing cell proliferation by releasing frHMGB1. The scaffold carries 1mm diameter of full-thickness rat skin-island as a minimal functional unit of skin (MFUS) to treat large full thickness skin wounds, and the hydrogel glues the MFUS and scaffold with skin edges together (MFUS+Scaffold group). The scaffold treated wounds (Scaffold group) heal much faster than the wounds either treated with MFUS (MFUS group) or without treatment (Wound group). The MFUS+Scaffold treated wound regenerates more functional full-thickness skin with more hair follicles and sweat glands, higher CD146 and α-smooth muscle actin levels, more blood vessels and collagen productions, and less scar tissues when compared to the other three groups. The results demonstrate that the combination of frHMGB1-LDI-Gly polymer with MFUS provides a new tissue engineering approach for large full-thickness skin wound healing.

Synthesis and degradation of 3D biodegradable polyurethane foam scaffolds based on poly (propylene fumarate) and poly[(R)-3-hydroxybutyrate

Tissue degeneration due to diseases, accidents, and wars is a worldwide issue. One of the methods used by regenerative medicine (RM) to aid tissue repair is by using a biodegradable tissue engineering scaffold (TES). Different body tissues have variable regeneration abilities, and hence, they require TES with variable degradation rates. This paper focuses on synthesising four polyurethane foam scaffolds (PUFS) with variable degradation rates based on the concentration of incorporated chain extender (CE) poly[(R)-3-hydroxybutyrate] (P3HB). The polyol employed is poly (propylene fumarate) (PPF) along with aliphatic 1,6-hexamethylene diisocyanate (HDI). GPC provided the molecular weight (Mn = 455.67 g mol−1, Mw = 587.15 g mol−1) and PDI (1.29) of the PPF. FTIR and 1H and 13C NMR confirmed the proposed structures (CC, COOR, and NHCOO) of the PUFS. The FESEM images revealed a bimodal pore structure, with random, irregular, interconnected pores (242–340 µm) and discrete void spaces (735–860 µm). The size of PUFS pores and void spaces (PU4 > PU3 > PU2 > PU1) is directly proportional to their respective degradation rates. The TGA/DTA curves show a decrease of an onset thermal decomposition temperature from PU1 to PU4 (267.17 ºC PU1, 267.28 ºC PU2, 256.01 ºC PU3 and 240.72 ºC PU4) due to the respective increase in CE concentration. The results of in-vitro degradation analysis at day 70, revealed significant difference between the weight loss (%) of each group (PU1 = 4.09 ± 0.18%, PU2 = 8.03 ± 0.17%, PU3 = 13.14 ± 0.12% and PU4 = 20.21 ± 0.19%). Thus, PUFS with a higher concentration of P3HB have a faster degradation rate due to the associated P3HB hydrolysable ester/-COOR functional group within the PUFS backbone. The chemical structure, bimodal morphology, and variable degradation rate of these PUFS could be employed for various RM applications.

Fabrication of Biodegradable Polyurethane Foam Scaffolds with Customized Shapes and Controlled Mechanical Properties by Gas Foaming Technique

AbstractPoly(ε‐caprolactone) (PCL)‐based polyurethane (PU) foam scaffolds with different mechanical properties are fabricated using a gas foaming technique to use as porous substitutes for ear or bone with cartilage. PCL diol or triol is used as a polyol in PU foam for biocompatibility and biodegradation, with an aqueous gelatin solution as a blowing agent. The highly porous inner and outer structures of the scaffolds are developed by employing a silicone surfactant and sulfuric acid, respectively. The PU scaffolds prepared by PCL diol show ductile and flexible properties, whereas the PU scaffolds prepared by PCL triol exhibit high compression strength. In vitro test reveals the low toxicity of the PU scaffolds and the high ALP activity of MC3T3‐E1 cells in the PU scaffold prepared by PCL triol. By taking advantage of the difference in mechanical properties, customized PU scaffolds with ear or bone shapes are fabricated using a silicone mold. The PU scaffolds with two compartments of PCL diol and triol (corresponding to cartilage and bone, respectively) are fabricated as a substitute for bone with cartilage. It is believed that the PU scaffolds with highly porous structure and controlled mechanical properties have wide potential application for tissue engineering.

The Effect of Pore Size Distribution and l-Lysine Modified Apatite Whiskers (HAP) on Osteoblasts Response in PLLA/HAP Foam Scaffolds Obtained in the Thermally Induced Phase Separation Process.

In this research, we prepared foam scaffolds based on poly(l-lactide) (PLLA) and apatite whiskers (HAP) using thermally induced phase separation technique supported by the salt leaching process (TIPS-SL). Using sodium chloride having a size of (a) 150–315 μm, (b) 315–400 μm, and (c) 500–600 μm, three types of foams with different pore sizes have been obtained. Internal structure of the obtained materials has been investigated using SEM as well as μCT. The materials have been studied by means of porosity, density, and compression tests. As the most promising, the composite prepared with salt size of 500–600 μm was prepared also with the l-lysine modified apatite. The osteoblast hFOB 1.19 cell response for the scaffolds was also investigated by means of cell viability, proliferation, adhesion/penetration, and biomineralization. Direct contact cytotoxicity assay showed the cytocompatibility of the scaffolds. All types of foam scaffolds containing HAP whiskers, regardless the pore size or l-lysine modification induced significant stimulatory effect on the cal-cium deposits formation in osteoblasts. The PLLA/HAP scaffolds modified with l-lysine stimulated hFOB 1.19 osteoblasts proliferation. Compared to the scaffolds with smaller pores (150–315 µm and 315–400 µm), the PLLA/HAP foams with large pores (500–600 µm) promoted more effective ad-hesion of osteoblasts to the surface of the biomaterial.

Preparation of a PLGA-coated porous bioactive glass scaffold with improved mechanical properties for bone tissue engineering approaches

The study aimed to improve the mechanical properties of a sol-gel-derived bioactive glass (BG) foam scaffold by poly-lactic co-glycolic acid (PLGA) coating as a potential scaffold for bone tissue regeneration. Polyurethane (PU) foam as the initial frame was submerged into a sol-gel-derived BG solution. The heat treatment process was carried out at 450°C to remove the PU foam and 860°C to consolidate the glass and remove the nitrate groups. The prepared BG scaffolds then were submerged into 10 and 15 wt% of PLGA solution. The uncoated BG scaffold was characterized by DTA/TGA, XRD, SEM, and TEM. The PLGA-coated scaffolds were characterized using SEM, and the mean compressive strength of the samples was measured. The DTA/TGA and XRD pattern indicated the removal of all nitrate groups at the temperatures above 700°C and also crystallization of BG after 800°C. The mean compressive strength of 10% and 15% PLGA-coated scaffolds were obtained 1.36±0.39 and 1.95±0.70 MPa, respectively, while the uncoated samples were crushed before the test. The SEM images exhibited that in the coated samples, the BG struts were covered with a PLGA layer and the coated layer was thicker in 15% PLGA samples. The SEM images also revealed that the PLGA coating maintained the structure of the broken struts and prevented them from coming apart. Thus, based on the obtained results, the PLGA coating was successfully toughened the BG scaffold and improved its elasticity. The study aimed to improve the mechanical properties of a sol-gel derived bioactive glass (BG) foam scaffold by Poly-lactic co-glycolic acid (PLGA) coating as a potential scaffold for bone tissue regeneration. Based on the results of this study, the PLGA coating can improve the elasticity and relieve the brittleness of sol-gel derived BG scaffold by supporting the fractured struts and prevent the scaffold from coming apart during a compressive strength test. Consequently, based on the obtained results a 15% PLGA-coated sol-gel derived BG scaffold can be considered as a potential scaffold for bone tissue regeneration.

In-situ growth of porous CoTe2 nanosheets array on 3D nickel foam for highly sensitive binder-free non-enzymatic glucose sensor

The combination of transition-metals with chalcogens provide a platform for developing highly sensitive and stable electro-catalyst materials possessing excellent electrochemical features regarding glucose oxidation. The growth of porous cobalt telluride (CoTe2) nanosheets (NSs) on a three-dimension (3D) nickel foam (NF) scaffold via anion-exchange transformation is achieved by employing low temperature scalable hydrothermal process. Being an active catalyst material for glucose detection, the CoTe2 NSs/NF electrode demonstrates an ultra-prompt response time of 0.1 s, boosting sensitivity of 168000 μA mM−1 cm−2, low limit of detection of 0.59 μM along with excellent anti-interference ability and favorable stability. Besides, the effective electrochemical performance of sensing electrode is recognized with respect to the glucose detection in real human blood serum. Overall, this material guarantees free-standing 3D architecture, interconnected porous NSs morphology, large specific surface area, high conductivity, and appealing electro-catalytic activity. Therefore, the porous CoTe2 NSs/NF binder-free electrode has a great application prospect as a promising biomimetic catalyst material for highly sensitive and efficient non-enzymatic glucose sensor.

Osteogenic differentiation of mesenchymal stem cells on the bimodal polymer polyurethane/polyacrylonitrile containing cellulose phosphate nanowhisker.

Polycaprolactone diol is the cornerstone, equipped with polyacrylonitrile and cellulose nanowhiskers (CNWs), of biocompatible and biodegradable polyurethanes (PUs). The solvent casting/particulate leaching technique was employed to contracting foam scaffolds with bimodal sizes from the combination of polyurethane/polyacrylonitrile/cellulose nanowhisker nanocomposites. Sugar and sodium chloride are components used as porogens to develop the leaching method and fabricate the 3D scaffolds. Incorporation of different percentages of cellulose nanowhisker leads to the various efficient structures with biodegradability and biocompatibility properties. All nanocomposites scaffolds, as revealed by MTT assay using mesenchymal stem cell (MSC) lines, were non-cytotoxic. PU/PAN/CNW foam scaffolds were used for osteogenic differentiation of human mesenchymal stem cells (hMSCs). Based on the results, PU/PAN/CNW nanocomposites could not only support osteogenic differentiation but can also enhance the proliferation of hMSCs in three-dimensional synthetic extracellular matrix.

Preparation of cellular SiBOC foams by precipitating methylvinylborosiloxane oligomers within melamine formaldehyde foam scaffold

SiBOC cellular ceramic foams were realized by precipitating methylvinylborosiloxane (MVBS) oligomers within reticulated poly(melamine-formaldehyde) (PMF) foam followed by drying and ceramization. The conventional method of impregnation-squeezing-drying exhibited MVBS starvation at the foam-core as the MVBS oligomers migrate to the surface due to its high solubility in ethanol and small cell size (200 ppi) of the PMF foam. In contrast, the precipitation of the MVBS oligomers on the web of the PMF foam prevents their migration to the surface. Ceramization by heat treatment at 1500 °C in inert atmosphere resulted in amorphous SiBOC cellular foams with cells in the size range of 20–400 μm. The density, compressive strength and thermal conductivity of the SiBOC foams were modulated in the ranges of 0.18−0.39 gcm−3, 0.3 to 0.87 MPa and 0.08−0.13 Wm-1 K-1, respectively, by using MVBS solution of concentrations in the range of 37–73 wt.%. The SiBOC foams remain amorphous up to 1600 °C beyond which extensive crystallization and phase separation occurs. Exposure of the SiBOC foam bodies at 1300 °C in air atmosphere showed negligible (∼ 0.2 wt.%) weight gain due to oxidation, indicating its potential as thermal protection material in oxidizing atmosphere.

Reduced graphene oxide foam templated by nickel foam for organ-on-a-chip engineering of cardiac constructs.

Myocardial tissue engineering has attracted increasing awareness for heart failure, and researchers are committed to developing an appropriate biological material to reconstruct myocardial tissues. Here, we applied a simple and high-throughput method to fabricate a three-dimensional (3D) partially reduced graphene oxide (PRGO) foam chip, whose structure, properties and biocompatibility confirmed that it is a suitable material for myocardial tissue engineering. The PRGO foam was produced based on a reduction reaction that occurred at the interface between the graphene oxide (GO) solution and Ni foam; as the Ni foam scaffold was dissolved in an HCl solution, the PRGO foam was harvested. After the PRGO foam was freeze-dried, its elasticity property was evaluated, and primary cardiomyocytes obtained from 2-day-old SD rats were cultured in the 3D foam. The results demonstrated good cell adherence, spreading, activity, organization and beating function in the PRGO foam during the long-term culturing process, which proved that the PRGO foam obtained by this method had application potential for myocardial tissue engineering.

Bioactive glass scaffold architectures regulate patterning of bone regeneration in vivo

The architecture of bone scaffolds, such as pore dimensions, connectivity and orientation can regulate osteogenic defect repair, as can their rate of degradation. Synthetic bone grafts have historically been developed with foam structures to mimic trabecular bone. Now, Additive Manufacturing techniques enable production of open and regular pore architectures with improved compressive strengths. Here, we compare two types of bioactive glass scaffolds, made of the highly biodegradable ICIE16 composition, with distinctively different architectures but matched interconnect sizes (~150 µm), produced via two different techniques: gel-cast foaming and direct ink writing. A rabbit lateral femoral defect model was used to compare the effect of their architecture on in vivo bone regeneration, relative to a defect only control group, after 4 and 10 weeks of implantation. 3D X-ray microcomputed tomography (micro-CT), correlated to histology and back-scatter electron microscopy (BS-SEM) permitted quantitative evaluation of new bone ingrowth and degradation of the scaffolds. Both foam and printed scaffolds showed equal or higher bone ingrowth compared to the control group. After 4 weeks, the foam group showed the highest osteogenesis, with 51% more bone ingrowth than the defect only controls, but after 10 weeks the defect treated with the printed scaffold had the most bone ingrowth (40% more than the empty defect). Energy dispersive X-ray (EDS) mapping revealed degradation of the glass and calcium-phosphate deposition. The foam group showed more rapid degradation than the printed group, due to higher total porosity (even though interconnected pore size was equivalent). The foam scaffold appeared to allow rapid bone ingrowth and cancellous bone formation, whereas the printed scaffold seemed to provoke cortical-like bone formation, while remaining in place for longer than the 10 week study. While the foam's concave architectures promote initial bone ingrowth, the higher strength open pore channels of the printed scaffolds are beneficial for scaffolds made of highly degradable bioactive glasses.

Self-standing zeolite foam monoliths with hierarchical micro-meso-macroporous structures.

The zeolite monoliths were synthesized by a facile polymer scaffold template assisted hydrothermal method. The selected foam-shaped template of a polyurethane (PU) foam monolith, was used to prepare the self-standing zeolite foam (ZF) monolithic materials. The obtained ZF products can preserve the same size, shape and macroporous network structure of the original PU foam scaffold template, although the zeolite nano-crystallites had been fully substituted for the PU template to form the new skeleton struts and walls. The as-synthesized ZF products demonstrated abundant hierarchical porosity (involving triple micro-, meso- and macropores). Meanwhile, compared with the conventional zeolite powders, the self-standing ZF monolithic materials exhibited greater total pore volume and nearly three times higher mesopore volume, suggesting wider applications as catalysts, catalyst supports and adsorbents in industry.

Fabrication and evaluation of gelatin/hyaluronic acid/chondroitin sulfate/asiatic acid based biopolymeric scaffold for the treatment of second-degree burn wounds – Wistar rat model study

An extracellular matrix (ECM) mimicking architecture was introduced with gelatin glycosaminoglycans like hyaluronic acid and chondroitin sulfate and a triterpenoid using asiatic acid, possessing biodegradable and biocompatible properties that mark the functionality for the treatment of second-degree burn wounds. In the present work, a foam-based scaffold was fabricated and sterilized with gamma radiation at a 2.5 Mrad dose. The scaffolds were further characterized for morphology, swelling, degradation behaviour, release of bioactive components, ATR-FTIR, mechanical, thermal properties and compared with control. In vitro cytocompatibility of the developed scaffold was studied with L929 mouse fibroblast cells and human mesenchymal stem cells based on deoxyribonucleic acid and lactate dehydrogenase assay. Additionally, the developed scaffold was evaluated for its biocompatibility on the Wistar rat to assess any toxicity induced to the animal based on blood biochemistry and histopathology analysis. Finally, we assessed the efficacy of developed foam scaffolds on the second-degree burn wound-induced Wistar rat with a scaffold alone and a scaffold seeded with human bone-marrow-derived mesenchymal stem cells in a wound healing study for 28 d. The wound contraction assay, histopathology, immunohistochemistry analysis and pro-healing marker quantification using hexosamine, hydroxyproline, and pro-inflammatory markers like TNF-α and MMP-2 were carried out and compared with the commercially available wound dressing. The results revealed that foam-based ECM mimic was cytocompatible, biocompatible and biodegradable in 18 ± 3 d in in vivo conditions and the scaffold fostered the process of healing of second-degree burns within 28 d of treatment. The obtained result proved that the scaffold has a potential for clinical settings in second-degree burn wound treatment.