Event Abstract Back to Event Protection from autoimmune nephritis in mice transgenic for BAFF, but lacking CD19 Kirsten A. Fairfax1* and Fabienne MacKay1 1 Monash University, Australia B cell activating factor belonging to the TNF family (BAFF or BLys) is required for B cell survival and maturation. However, mice transgenic for BAFF (BAFFTg) develop spontaneous autoimmunity, resembling Systemic Lupus Erythematosus (SLE), in a T cell-independent manner. The autoantibodies which trigger disease in BAFFTg mice, are produced by marginal zone (MZ) and B1 cells, but not follicular B cells. Experiments using BAFFTg splenectomized mice suggested that it was not MZ or B1a B cells, but rather B1b B cells that were important in the development of autoimmune nephritis. We have developed a mouse cross between BAFFTG and CD19 knockout (ko) mice, in which MZ, B1a and B1b B cells are reduced. We observed that BAFFTgxCD19ko mice had reduced titres of autoantibodies compared to BAFFTg mice, and these mice resembled CD19ko animals with respect to autoantibody development. The BAFFTgxCD19ko animals were also protected from the splenomegaly observed in the BAFFTg animals. In aged mice BAFFTg display complement deposition in the glomeruli within the kidney, whilst BAFFTgxCD19ko were protected from this (as were the CD19ko mice). In addition, the BAFFTg glomeruli were larger, and more segmented than the BAFFTgxCD19ko, both signs of impaired function. Together these results point towards the pathogenic role of B1b B cells in autoimmune nephritis. This work also raises the prospect that targeting CD19 in SLE patients may prove efficacious. Acknowledgements We would like to acknowledge the Monash MicroImaging Facility, the AMREP Flow facility, the AMREP animal facility and the Baker Institute for allowing us to use the Cobas instrument. Keywords: Autoimmunity, BaffTg, Lupus Nephritis, B1 Cells, MZ B cells Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Fairfax KA and MacKay F (2013). Protection from autoimmune nephritis in mice transgenic for BAFF, but lacking CD19. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00930 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Kirsten A Fairfax, Monash University, Melbourne, Australia, kirsten.fairfax@monash.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kirsten A Fairfax Fabienne MacKay Google Kirsten A Fairfax Fabienne MacKay Google Scholar Kirsten A Fairfax Fabienne MacKay PubMed Kirsten A Fairfax Fabienne MacKay Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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