Using the conjugation symmetry on Heegaard Floer complexes, we define a three-manifold invariant called involutive Heegaard Floer homology, which is meant to correspond to $\mathbb{Z}_4$-equivariant Seiberg-Witten Floer homology. Further, we obtain two new invariants of homology cobordism, $\underline{d}$ and $\bar{d}$, and two invariants of smooth knot concordance, $\underline{V}_0$ and $\overline{V}_0$. We also develop a formula for the involutive Heegaard Floer homology of large integral surgeries on knots. We give explicit calculations in the case of L-space knots and thin knots. In particular, we show that $\underline{V}_0$ detects the non-sliceness of the figure-eight knot. Other applications include constraints on which large surgeries on alternating knots can be homology cobordant to other large surgeries on alternating knots.
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