Final Separation Research Articles

Recovery of Cenospheres and Fine Fraction from Coal Fly Ash by a Novel Dry Separation Method

Aluminosilicate microspheres are a valuable fraction of coal fly ash with diverse applications due to their low density. Currently, there is no efficient and ecologically rational method of cenosphere recovery from fly ash. A combination of dry methods for the recovery of both fine ash particles and aluminosilicate microspheres from coal fly ash is presented. It is comprised of fluidised bed separation followed by screening and pneumatic separation in a free-fall air chamber. Fluidised bed separation was assisted by a mechanical activator to prevent agglomeration. This step reduced the portion of material that required further treatment by 52–55 wt.%, with the recovery of microspheres exceeding 97%. Then, the concentrates were individually subjected to pneumatic separation. The final separation product for the fly ash containing 0.64 wt.% cenospheres was a cenosphere concentrate that constituted about 17 wt.% of the initial fly ash. The recovery of cenospheres was around 81%. Usage of a combination of dry methods allowed for maintaining almost 83 wt.% of the raw material in its dry form. Furthermore, the produced fly ash grain fractions could be used for different industrial purposes.

Residual relief modelling: digital elevation enhancement for shipwreck site characterisation

Scour processes play a critical role in the preservation status of submerged historic shipwrecks. Erosion of sediment leads to enhanced exposure of archaeological sites to physical, chemical and biological processes. Current methods for identifying erosional and depositional features at wreck sites are based primarily on visual interpretation of data, which is labour-intensive and entirely subjective. The increasing availability of high-resolution multibeam echosounder–derived digital elevation models (DEMs) of historic wreck sites allows for an entirely new level of detailed interrogation and analyses of the geomorphological features associated with these. In this study, we present a residual relief modelling method for the semi-automated extraction of such depositional and erosional features at wreck sites. Relief modelling is supplemented with a breakpoint classification approach, with final separation supported by DEM visualisation enhancement techniques. We applied the method to three World War I shipwreck sites and evaluated it against traditional manual vectorisation techniques. The results suggest that the semi-automated modelling method is robust, time-effective and capable of quantifying the products of scour processes with increased objectivity. Our method holds great potential for the objective characterisation of erosional and depositional patterns and processes at wreck sites, which have important implications for site formation studies and in situ preservation of underwater cultural heritage.

Isolation of human CD45+ leukocytes from tissues and human tumor xenografts in humanized mice

Abstract The study of immune cell function in non-lymphoid tissue and tumors promises to elucidate novel strategies to treat immune disorders, infectious diseases, and cancer. To address the challenge of isolating leukocytes from complex and variable tissues and tumors, we have developed a new protocol to isolate particle-free, human CD45+ leukocytes. Using the EasySep™ Release Human CD45 Positive Selection Kit, leukocytes are labeled with antibody complexes linked to magnetic particles and separated using an EasySep™ magnet. The magnetic particles are then removed from the desired cells by resuspension in EasySep™ Release Buffer and a final magnetic separation. To assess performance, NRG-3GS mice were first engrafted with human CD34+ cells followed by xenotransplant with human breast (MDA-MB-231) or ovarian (SKOV3) cancer cell lines. In humanized mouse lungs, bone marrow and spleen, the starting and isolated human CD45+ frequency ranges were 6.0 – 57.2% and 90.9 – 99.4%, respectively (n = 3). Starting with human tumor xenografts, tumor infiltrating leukocytes were enriched from a starting range of 0.4 – 18.0% to 76.6 – 92.7% (n = 4). The final immune cell frequencies are representative of the starting population, and further separation of immune subsets can be achieved with additional downstream isolation. Humanized mouse models of clinical disease are instrumental in furthering our understanding of complex mechanisms of disease progression and resolution. This new kit for the isolation of human immune cells from tissues and tumors will facilitate further examination of the roles of immunity in disease and the evaluation of immune-based treatment strategies.

Eocene Araucaria Sect. Eutacta from Patagonia and floristic turnover during the initial isolation of South America.

Eocene floras of Patagonia document biotic response to the final separation of Gondwana. The conifer genus Araucaria, distributed worldwide during the Mesozoic, has a disjunct extant distribution between South America and Australasia. Fossils assigned to Australasian Araucaria Sect. Eutacta usually are represented by isolated organs, making diagnosis difficult. Araucaria pichileufensis E.W. Berry, from the middle Eocene Río Pichileufú (RP) site in Argentine Patagonia, was originally placed in Sect. Eutacta and later reported from the early Eocene Laguna del Hunco (LH) locality. However, the relationship of A. pichileufensis to Sect. Eutacta and the conspecificity of the Araucaria material among these Patagonian floras have not been tested using modern methods. We review the type material of A. pichileufensis alongside large (n = 192) new fossil collections of Araucaria from LH and RP, including multi-organ preservation of leafy branches, ovuliferous complexes, and pollen cones. We use a total evidence phylogenetic analysis to analyze relationships of the fossils to Sect. Eutacta. We describe Araucaria huncoensis sp. nov. from LH and improve the whole-plant concept for Araucaria pichileufensis from RP. The two species respectively resolve in the crown and stem of Sect. Eutacta. Our results confirm the presence and indicate the survival of Sect. Eutacta in South America during early Antarctic separation. The exceptionally complete fossils significantly predate several molecular age estimates for crown Eutacta. The differentiation of two Araucaria species demonstrates conifer turnover during climate change and initial South American isolation from the early to middle Eocene.

Investigation of a two-step device implementing magnetophoresis and dielectrophoresis for separation of circulating tumor cells from blood cells.

Identifying tumor cells from a pool of other cells has always been an appealing topic for different purposes. The objective of this study is to discriminate circulating tumor cells (CTCs) from blood cells for diagnostic purposes in a novel microfluidic device using two active methods: magnetophoresis and dielectrophoresis. The most specific feature of this device is the differentiation of CTCs without labeling them in order to achieve a more reliable and less complicated method. This device was analyzed and evaluated using finite element method. Four cell lines are separated in this device containing red blood cells, platelets, white blood cells, and CTCs. Primarily, red blood cells and platelets, which constitute the largest part of a blood sample, are removed in the magnetophoresis section. Remaining cells enter the dielectrophoresis part and based on their inherent dielectric properties and diameters, final separation occurs. In each step, different parameters are examined to obtain the maximum purification. The results demonstrate the potential of different CTCs separation by changing the effective parameters in the designed device based on the inherent properties of the cells.

Integration of Solid-Phase Extraction and Reversed-Phase Chromatography in Single Protein-Coated Columns for Direct Injection of Bupivacaine in Human Serum.

A rapid, reliable and precise integrated solid-phase extraction (SPE) and reversed-phase liquid chromatography method was developed and validated to determine bupivacaine in human serum using single protein-coated analytical columns. The protein-coated columns were packed with four different sorbents: TSK-ODS, LiChrosorb RP-8, LiChrosorb RP-2 and μ-Bondapak CN-bonded silica. The method involved direct injection of serum sample onto the columns for trapping of the analyte, clean-up from weakly retained serum endogenous components, as well as the final separation. The protein-coated columns operated in two different chromatographic modes. Serum proteins were extracted and cleaned up by SPE, whereas the final separation of bupivacaine was based on reversed-phase chromatography. The protein-coated TSK-ODS column resulted in more accurate peak integration and more reproducible results. A linear relationship between the concentrations of drug and peak areas was confirmed in the range of 100-2000ng/mL. Detection and quantification limits were 24.85 and 85.36ng/mL, respectively. The average recovery for bupivacaine ranged from 96.48% to 98.81%. The present methodology was successfully applied, with a high degree of confidence, to analyze clinical samples obtained from patient receiving 0.5% bupivacaine therapy.

The impact of recombination energy on simulations of the common-envelope binary interaction

ABSTRACT During the common-envelope binary interaction, the expanding layers of the gaseous common envelope recombine and the resulting recombination energy has been suggested as a contributing factor to the ejection of the envelope. In this paper, we perform a comparative study between simulations with and without the inclusion of recombination energy. We use two distinct setups, comprising a 0.88- and 1.8-M⊙ giants, that have been studied before and can serve as benchmarks. In so doing, we conclude that (i) the final orbital separation is not affected by the choice of equation of state (EoS). In other words, simulations that unbind but a small fraction of the envelope result in similar final separations to those that, thanks to recombination energy, unbind a far larger fraction. (ii) The adoption of a tabulated EoS results in a much greater fraction of unbound envelope and we demonstrate the cause of this to be the release of recombination energy. (iii) The fraction of hydrogen recombination energy that is allowed to do work should be about half of that which our adiabatic simulations use. (iv) However, for the heavier star simulation, we conclude that it is helium and not hydrogen recombination energy that unbinds the gas and we determine that all helium recombination energy is thermalized in the envelope and does work. (v) The outer regions of the expanding common envelope are likely to see the formation of dust. This dust would promote additional unbinding and shaping of the ejected envelope into axisymmetric morphologies.

Common Envelope Evolution on a Moving Mesh

The common envelope phase in binary star systems is simulated using the 3-D moving-mesh hydrodynamic code MANGA. Improvements to MANGA to improve accuracy and computation time are discussed. Two open questions in the physics of common envelope evolution are investigated. The effects of tidal forces present before the onset of a common envelope phase are explored by comparing simulations in which the giant star is initialized with varying degrees of rotation. The role of hydrogen recombination energy is investigated by using two different equations of state, only one of which includes the effects of recombination. Rotation is shown to increase the final binary separation, while recombination energy decreases the separation. Future improvements to MANGA to capture additional physics present in common envelopes are discussed.

Column Separation of Am(III) and Eu(III) by α-Zirconium Phosphate Ion Exchanger in Nitric Acid

The trivalent lanthanide-actinide separations are a major challenge in reprocessing of nuclear fuels. To achieve this, commonly organic extractants and solvents are utilized in elaborate processes. Here we report a simple new method that can perform a supportive or alternative role. A nanocrystalline α-zirconium phosphate ion exchanger was utilized for Eu(III)/Am(III) column separation. Comprehensive preliminary studies were done using batch experiments to optimize the final separation conditions. The distribution coefficients for Eu were determined as a function of pH (from 0 to 3) and salinity (Na, Sr). The distribution coefficients for Am were determined as a function of pH, and Eu concentration, from 1:40 to 10,000:1 Eu:Am molar ratio. The exchanger always preferred Eu over Am in our experimental conditions. Separation factors (Eu:Am) of up to 400 were achieved in binary Eu-Am solution in pH 1. The breakthrough capacity was determined in dynamic column conditions using Eu: 0.3 meq∙g−1, which is approximately 4% of the theoretical maximum capacity. Two types of hot column separation tests were conducted: (i) binary load (selective Am elution), and (ii) continuous equimolar binary feed. In both cases separation was achieved. In (i), the majority (82% of the recovered 93%) of Am could be purified from Eu with extremely high 99.999% molar purity, while alternatively even more (95% of the recovered 93%) at a lower purity of 99.7 mol %. In (ii), up to 330 L∙kg−1 of the equimolar solution per mass of the exchanger could be treated with Am purity above 99.5 mol % in the total eluate. Alternatively, up to 630 L∙kg−1 above 95 mol %, or up to 800 L∙kg−1 above 90 mol % purities.

Genesis of women movement in the context of gender relationsin Ukraine (second half of XIX - beginning of XX century)

The purpose and tasks of the article are to summarize and analyze the organizational forms of the genesis of the women's movement in Ukraine in the second half of the nineteenth - early twentieth centuries; highlighting their specificity in the time intervals proposed by the authors in each of the three stages; opening on the basis of the first organized women's groups and societies in the Dnieper Ukraine and the Western Ukrainian lands of joint orientation of actions in realization of tasks of achievement of gender equality.
 On the first - emancipatory - there is a process of origin and formation of women's movement at first thanks activities of S. Kovalevska, E. Miloradovych, H. Alchevska and other prominent Ukrainian women. The activity is carried out by charity, dissemination of education, etc. From the mid-80's the first organized circles were formed O. Dobrogaeva and N. Kobrynska. At the same time, close ties are being established with leading Ukrainian figures I. Franko, M. Drahomanov and Ukrainian-language publishing activity is being established.
 The second stage is characterized by the formation of women's societies of moral and ethical and socio-political direction. Their number is growing significantly. The politicization of the women's movement is becoming widespread. Expanding political slogans in his activities. At the First All-Russian Congress, two women's currents are being formed movement: liberal-democratic and social-democratic.
 The third is characterized by some weakening of women's societies due to the reaction in society, the final separation of the two currents in the women's movement and the outflow of some activists to the ranks of political parties.
 The revival of the movement began in 1909 with the formation of a new type of organization - independent organizations feminist orientation - Women's Clubs. Their activities and representation in the All-Russian and international forums. Women received partial rights in the socio-political and social spheres.
 It is emphasized that legal changes, even inconsistent ones, in the Russian and Austro-Hungarian empires have had a definite impact on the process of Ukrainian women's rethinking their position and the spread of emancipatory ideas; more women's access to education; familiarizing them with civilizational approaches to the women's issue of domestic and foreign figures. It is proved that the development of organizational forms of the women's movement and its politicization were influenced by factors of general politicization and the development of the general social-political movement.

Applied Process Simulation-Driven Oil and Gas Separation Plant Optimization Using Surrogate Modeling and Evolutionary Algorithms

In this article, the optimization of a realistic oil and gas separation plant has been studied. Using Latin Hypercube Sampling (LHS) and rigorous process simulations, surrogate models using Kriging have been established for selected model responses. The surrogate models are used in combination with an evolutionary algorithm for optimizing the operating profit, mainly by maximizing the recoverable oil production. A total of 10 variables representing pressure and temperature at various key places in the separation plant are optimized to maximize the operational profit. The optimization is bounded in the variables and a constraint function is included to ensure that the optimal solution allows export of oil with a Reid Vapor Pressure (RVP) < 12 psia. The main finding is that, while a high pressure is preferred in the first separation stage, apparently a unique optimal setting for the pressure in downstream separators does not appear to exist. In the second stage separator, apparently different, yet more or less equally optimal, settings are revealed. In the third and final separation stage a correlation between the separator pressure and the applied inlet temperature exists, where different combinations of pressure and temperature yields equally optimal results.

Statistical optimization and validation of a novel ultra-performance liquid chromatography method for estimation of nintedanib in rat and human plasma.

Aim: A high throughput ultra-performance liquid chromatography (UPLC)-ultraviolet method for quantification of nintedanib in rat and human plasma was developed and optimized using chemometrical approach. Method: Design of experiment and multivariate statistical approach was used for definition of optimized method. Final separation was performed using protein precipitation method on ACQUITY HSS T3 C18 column in isocratic mode using potassium phosphate buffer (pH 7.5): acetonitrile. Results: Method was validated as per US-FDA guidelines linearly from 15-750ng/ml. All quality control samples showed <15% relative standard deviation for precision and 85-115% accuracy along with >98% extraction recovery. Conclusion: The developed method is easily applicable in determining pharmacokinetic parameters in preclinical subjects along with successful implementation for quantification in human plasma samples.

Experimental Investigation on Mechanism of Latent Heat Reduction of Sodium Acetate Trihydrate Phase Change Materials.

Sodium acetate trihydrate (SAT) phase change material (PCM) has been well known for thermal energy storage due to its high latent heat and resource abundance. However, SAT suffers from severe latent heat reduction after heating and cooling cycles. Although a few of previous researches showed the reduction could be effectively inhibited by using thickeners, the mechanisms of the reduction process and thickeners’ inhibition have not been deeply explored till now. In this work, SAT modified by 5 wt.% nucleating agent of disodium hydrogen phosphate dodecahydrate (SAT/5 wt.% DSP) was prepared and 200 thermal cycles were carried out. The differential scanning calorimeter, Rheometer, X-ray diffractometry, and scanning electron microscope were used to investigate the extent of latent heat reduction, viscosity, phase composition and microstructure, respectively, and the infrared thermal imaging method was used to evaluate heat storage capacity. It was found that the latent heat of SAT/5 wt.% DSP dropped dramatically and the relative decrease in latent heat was measured to be 22.44%. The lower layer of SAT/5 wt.% DSP contained 24.1 wt.% CH3COONa, which was quantitatively consistent with the reduction extent. Furthermore, the phase change endothermic time of the lower layer was only 44.1% of that of the upper. SAT/5 wt.% DSP was further modified by 3 wt.% thickener of carboxymethyl cellulose (SAT/5 wt.% DSP/3 wt.% CMC) and endured 200 thermal cycles. The extent of the latent heat reduction of SAT/5 wt.% DSP/3 wt.% CMC was only 9.29%, and phase compositions were more homogeneous. The 3 wt.% CMC increased viscosity by 14 times, which effectively prevented the Stokes sedimentation velocity of CH3COONa in melts and inhibited the final macroscopic phase separation.

Shaping of the nephron \u2013 a complex, vulnerable, and poorly explored backdrop for noxae impairing nephrogenesis in the fetal human kidney

BackgroundThe impairment of nephrogenesis is caused by noxae, all of which are significantly different in molecular composition. These can cause an early termination of nephron development in preterm and low birth weight babies resulting in oligonephropathy. For the fetal human kidney, there was no negative effect reported on the early stages of nephron anlage such as the niche, pretubular aggregate, renal vesicle, or comma-shaped body. In contrast, pathological alterations were identified on subsequently developing S-shaped bodies and glomeruli. While the atypical glomeruli were closely analyzed, the S-shaped bodies and the pre-stages received little attention even though passing the process of nephron shaping. Since micrographs and an explanation about this substantial developmental period were missing, the shaping of the nephron in the fetal human kidney during the phase of late gestation was recorded from a microanatomical point of view.ResultsThe nephron shaping starts with the primitive renal vesicle, which is still part of the pretubular aggregate at this point. Then, during extension of the renal vesicle, a complex separation is observed. The medial part of its distal pole is fixed on the collecting duct ampulla, while the lateral part remains connected with the pretubular aggregate via a progenitor cell strand. A final separation occurs, when the extended renal vesicle develops into the comma-shaped body. Henceforth, internal epithelial folding generates the tubule and glomerulus anlagen. Arising clefts at the medial and lateral aspect indicate an asymmetrical expansion of the S-shaped body. This leads to development of the glomerulus at the proximal pole, whereas in the center and at the distal pole, it results in elongation of the tubule segments.ConclusionsThe present investigation deals with the shaping of the nephron in the fetal human kidney. In this important developmental phase, the positioning, orientation, and folding of the nephron occur. The demonstration of previously unknown morphological details supports the search for traces left by the impairment of nephrogenesis, enables to refine the assessment in molecular pathology, and provides input for the design of therapeutic concepts prolonging nephrogenesis.

Toward an efficient multi-step separation protocol to recover metallic components from waste leachates

This work presents a study of the separation and recovery of metal elements, through a multi-step protocol based on solvent extraction, stripping and chemical precipitation processes. The waste leachate was produced during the treatment of the mixed solid waste from the decommissioning of a coal-fired power plant. The organophosphorus acid, di-(2-ethylhexyl) phosphoric acid (DEHPA) was investigated as organic extracting agent during the solvent extraction (Step 1). A factorial design with three levels for the organic phase/aqueous phase ratio (O/W), DEHPA concentration and contact time were carried out and a prediction model obtained using a neural-fuzzy approach. Optimized extraction values for Ti (93.4 %), (V) 94.6 % and (Zn) 89.8 % were obtained using 60min of contact time, a DEHPA concentration of 0.5M and O/W ratio of 4. Stripping tests were performed using HCl, H2SO4, NaOH and KOH as stripping agents. The stripping step (step 2) showed optimum results (82 % of Zn and 31 % of V) using 1M H2SO4 solution as stripping agent. A selective precipitation step (step 3) allowed the final separation of metals in aqueous solutions produced in Step 1 and 2. The results showed that an effective separation of Mn and Ni could be carried out at pH 9, while the best separation results for the solution obtained during the stripping step, which contains V and Ti, was obtained at pH 12.

Influence of species and processing parameters on recovery and content of brain tissue-derived extracellular vesicles

ABSTRACT Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processes by shuttling material out of and between cells. Tissue EVs may thus lend insights into disease mechanisms and also betray disease when released into easily accessed biological fluids. Since brain-derived EVs (bdEVs) and their cargo may serve as biomarkers of neurodegenerative diseases, we evaluated modifications to a published, rigorous protocol for separation of EVs from brain tissue and studied effects of processing variables on quantitative and qualitative outcomes. To this end, size exclusion chromatography (SEC) and sucrose density gradient ultracentrifugation were compared as final separation steps in protocols involving stepped ultracentrifugation. bdEVs were separated from brain tissues of human, macaque, and mouse. Effects of tissue perfusion and a model of post-mortem interval (PMI) before final bdEV separation were probed. MISEV2018-compliant EV characterization was performed, and both small RNA and protein profiling were done. We conclude that the modified, SEC-employing protocol achieves EV separation efficiency roughly similar to a protocol using gradient density ultracentrifugation, while decreasing operator time and, potentially, variability. The protocol appears to yield bdEVs of higher purity for human tissues compared with those of macaque and, especially, mouse, suggesting opportunities for optimization. Where possible, perfusion should be performed in animal models. The interval between death/tissue storage/processing and final bdEV separation can also affect bdEV populations and composition and should thus be recorded for rigorous reporting. Finally, different populations of EVs obtained through the modified method reported herein display characteristic RNA and protein content that hint at biomarker potential. To conclude, this study finds that the automatable and increasingly employed technique of SEC can be applied to tissue EV separation, and also reveals more about the importance of species-specific and technical considerations when working with tissue EVs. These results are expected to enhance the use of bdEVs in revealing and understanding brain disease.

CONCEPT OF SUPPLY CHAIN MANAGEMENT

The study aims to illustrate the scientist's view of the theoretical background related to supply chain management. The evolution of the development of this concept was considered and three main stages the 80s, the formulation of the concept and the idea of coordinating the actions of companies within the chain; 90s, the idea of integrating key business functions, the idea of focusing on the needs of end customers and other kinds of competition - between chains, and not between individual firms, the final separation of the concept of logistics from SCM; supply chain management concept transformation. An attempt was made to analyze the work of authors who considered the concept of supply chain management from philosophy, discipline, and process. In conclusion, it was concluded that the main views and opinions relate to the process approach.

Marine species formation along the rise of Central America: The anomuran crab Megalobrachium.

The evolution of marine neotropical shallow water species is expected to have been greatly affected by physical events related to the emergence of the Central American Isthmus. The anomuran crab Megalobrachium, a strictly neotropical porcellanid genus, consists of four species in the West Atlantic (WA) and nine in the East Pacific (EP). Dispersal is limited to a relatively short planktonic phase, which lasts approximately two weeks. We obtained DNA sequences of three mitochondrial and two nuclear genes of all but one species of Megalobrachium to construct a time-calibrated phylogeny of the genus and its historical phylogeography, based on the reconstruction of ancestral areas. The topology of the phylogenetic trees of Megalobrachium produced by Bayesian Inference (BI) and Maximum Likelihood (ML) were virtually congruent. The genus is monophyletic with respect to other porcellanids. Ancestral area reconstruction indicates that it arose in the eastern Pacific 18 million years ago and diversified into at least 13 species that are currently formally recognized and three additional species indicated by our data. Most morphological variation appears to have followed phylogenetic differentiation, though some cryptic speciation has also occurred. Four geminate clades in this genus implicate the gradual emergence of the Central American Isthmus in this diversification, but events preceding the final separation of the oceans as well as within-ocean events after the cessation of water connections were also important.

Induced spins from scattering experiments of initially nonspinning black holes

When two relativistically boosted, nonspinning black holes pass by one another on a scattering trajectory, we might expect the tidal interaction to spin up each black hole. We present the first exploration of this effect, appearing at fourth post-Newtonian order, with full numerical relativity calculations. The basic setup for the calculations involves two free parameters: the initial boost of each black hole and the initial angle between the velocity vectors and a line connecting the centers of the black holes, with zero angle corresponding to a head-on trajectory. To minimize gauge effects, we measure final spins only if the black holes reach a final separation of at least $20M$. Fixing the initial boost, we find that as the initial angle decreases toward the scattering/nonscattering limit, the spin-up grows nonlinearly. In addition, as initial boosts are increased from $0.42c$ to $0.78c$, the largest observed final dimensionless spin on each black hole increases nonlinearly from $0.02$ to $0.20$. Based on these results, we conclude that much higher spin-ups may be possible with larger boosts, although achieving this will require improved numerical techniques.

Effect of orbital eccentricity on the dynamics of precessing compact binaries

We study precession dynamics of generic binary black holes in eccentric orbits using an effective potential based formalism derived in [M. Kesden et al., PRL {\bf 114}, 081103 (2015)]. This effective potential is used to classify binary black holes into three mutually exclusive spin morphologies. During the inspiral phase, binaries make transitions from one morphology to others. We evolve a population of binary black holes from an initial separation of $1000\mathbf{M}$ to a final separation of $10\mathbf{M}$ using post-Newtonian accurate evolution equations. We find that, given suitable initial conditions, a binary's eccentricity can follow one of three distinct evolutionary patterns: (i) eccentricity monotonically increasing until final separation, (ii) eccentricity rising after decaying to a minimum value, and (iii) eccentricity monotonically decreasing throughout the inspiral. The monotonic growth or growth after reaching a certain minimum of eccentricity is due to the effect of 2PN spin-spin coupling. Further, we investigate the morphology transitions in eccentric binaries and find that the probability of such binaries transiting from one to other is similar to those in circular orbits, implying that eccentricity plays a sub-dominant role in spin morphology evolution of a precessing binary black hole. We, hence, argue that the morphological classification of spin precession dynamics is a robust tool to constrain the formation channels of binaries with arbitrary eccentricity as well.