To achieve excellent biocompatibility of metal-based implants, hydroxyapatite thin film coatings on nanotube-formed Ti–35Nb–10Zr alloys after femtosecond laser texturing have been investigated. Three groups of samples were prepared: (1) microtextured surfaces using femtosecond (FS) laser, (2) nanotube surfaces formed by anodization, and (3) hydroxyapatite coatings vapor-deposited by a physical electron beam method on the Ti–35Nb–10Zr ternary alloy. The surface morphology of the Ti–35Nb–10Zr alloys was examined by x-ray diffractometry, field-emission scanning electron microscopy, and energy-dispersive x-ray spectroscopy. To investigate ion release, potentiodynamic polarization testing was carried out in 0.9%NaCl solution, and wettability tests and MG 63 osteoblast-like cell culture studies were performed on the surfaces of the three groups to evaluate contact angle and cell growth morphology, respectively. The Ti–35Nb–10Zr alloys exhibited the equiaxed β phase structure. The FS laser-treated Ti–35Nb–10Zr alloys showed circular traces with diameters of 28±1.5μm and hole separations of 50μm, and the nanotubes formed on the surfaces had diameters of 180±15nm and compositions consistent with TiO2, Nb2O5, and ZrO2. The nanotube structure showed anatase, rutile and β-titanium diffraction peaks, whereas the HA-coated surface on the nanotubes showed hydroxyapatite, anatase and rutile peaks. The HA coatings displayed the desired role of decreasing the metal ion release with a lower current density value, and the nanotubes and FS laser-treated surface showed wider passive regions compared to other surfaces. The HA-coated surface on the nanotubes after FS laser texturing showed the lowest contact angle compared with the other surfaces. From FE-SEM observations, cell attachment and spreading of MG 63cells showed significantly higher tendency for surfaces covered by HA coating and nanotubes.