AbstractA class of bioactive, stimuli‐responsive co‐joined interpenetrating networks of inherently conductive polymers and highly hydrated hydrogels are being developed for implantable biodevice interfaces and for electric field induced release of elutable drugs. A novel microfabricated multidisc electrode array biotransducer intended for trauma management has been coated with poly(HEMA)‐polypyrrole and characterized by cyclic voltammetry and chronoamperometry using ferrocene monocarboxylic acid (FcCOOH) as a probe molecule. Electrodeposition of polypyrrole (700 mV vs. Ag/AgCl) to 100 mC/cm2 onto the hydrogel coated microdisc electrode array resulted in large and unstable background currents relative to uncoated electrodes. Overoxidation of polypyrrole (0–1.2 V vs. Ag/AgCl, 20 cylces, 100 mV/s) eliminates background current. Dose‐response curves with FcCOOH showed that the transducer has good reproducibility with molecules of facile electrochemical properties. Polypyrrole provides interference screening of endogenous interferents in biosensor applications with a 12:1 rejection ratio. GOx was immobilized via electropolymerization of polypyrrole into hydrogel coated MDEA 5037s to yield biotransducers with sensitivity of 0.045 µA mM−1 cm−2. Changes to improve biotransducer sensitivity are proposed.