Feed Residues Research Articles

Sensitivity of an improved method for post-life laboratory diagnosis of chicken eimeriosis

Poultry farming is vital in providing the population with high-quality food products – eggs and poultry meat. Poultry farming is characterized by rapid livestock reproduction rates, the lowest per unit of output compared to other livestock industries. To maintain high economic performance and produce high-quality products, it is necessary to ensure veterinary well-being concerning invasive diseases. The dangerous protozoan disease eimeriosis cause significant financial losses, high mortality of young poultry, decreased chicken productivity, and the quality of products from sick birds. The work aimed to improve and determine the sensitivity of the method of post-life laboratory diagnostics of eimeriosis in chickens. The proposed useful model relates to veterinary medicine, namely, veterinary parasitology, to methods of ovoscopic studies of chicken droppings, particularly a quantitative method for detecting eimeriosis oocysts. It was found that when examining chicken droppings for the presence of Eimeria oocysts, the most effective method was the improved method, which is based on using a combined hypertonic solution with a high specific gravity and well-defined coagulation properties. When using it, it was possible to detect the most significant number of oocysts – 1285.20 oocysts/g of droppings, which was 8.24 % more compared to the analog method (the method of quantitative coproscopic diagnostics of nematodoses of the digestive tract of ruminants) and 33.17 % (P < 0.01) – compared to the known method (Stall's method). Also, the improved method is the most optimal regarding the number of positive samples, the number of detected invasive elements in 1 g of droppings, and the coagulation ability of the flotation solution concerning undigested feed residues detected during microscopy. The obtained research results allow us to recommend implementing the proposed method for quantitative detection of Eimeria sp. oocysts in chicken droppings for effective, sensitive, and timely laboratory diagnostics of Eimeria sp.

QuEChERS methodology and Gas Chromatography-mass spectrometry for detection of 345 pesticide residues in Egyptian poultry feed: a comprehensive study

Animal feed significantly influences the content and quality of animal products consumed by humans and plays a crucial role in the food chain. To simultaneously analyze 345 pesticide residues in poultry feed, a modified Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method was developed, utilizing gas chromatography-tandem mass spectrometry (GC-MS/MS). Validation studies were conducted with spiked pesticide standards, and commercial poultry feed samples were analyzed. The linearity of the QuEChERS method was evaluated, showing high coefficients of determination (R 2 ≥0.995) for all analytes. The method demonstrated excellent linearity (R 2 ≥0.995), recovery rates (86.8–109.2%), and precision (RSD ≤14.8%), meeting SANTE criteria. Limits of detection (LOD) ranged from 0.5 to 5 ng/kg and limits of quantification (LOQ) from 1 to 10 ng/kg, enhancing the method’s reliability. Analysis of the poultry feed revealed that 15 out of the 345 pesticide residues were detected in the samples, with concentrations ranging from 2.1 to 120 µg/kg. The detected pesticide categories included insecticides, fungicides, plant growth regulators, and herbicides, in that order. In conclusion, the QuEChERS method combined with the GC-MS/MS approach provides a reliable technique for the multi-residue analysis of pesticides in complex feed matrices.

Effects of the pesticide carbendazim on broiler chicken health and carbendazim residue levels in broiler tissues.

Pesticide residues may enter the human body through the food chain when livestock and poultry consume pesticide-contaminated feed. Therefore, monitoring and limiting pesticide residues in animal feed and animal-origin foods is necessary. Carbendazim is one of the most frequently detected pesticides in food and feed and has various toxic effects on non-target animals. This study investigated the effects of varying concentrations of carbendazim contamination in feed on broiler chicken growth performance, serum biochemical indicators, histopathology, and carbendazim residues in broiler muscles and livers. The results demonstrated that contamination of 5-100mg/kg carbendazim in feed did not affect broiler growth performance or health. Carbendazim contamination in feed at 200-800mg/kg slightly reduced growth performance. Broiler kidneys showed minor histopathological alterations after 400mg/kg carbendazim exposure. Furthermore, when the carbendazim content in feed was less than 25mg/kg, the residual carbendazim in the muscles and livers of broilers did not exceed the maximum residue level set by the European Union and China. Based on the above findings, carbendazim residues in the feed of less than 25mg/kg can be considered safe for chicken products.

A colloidal gold immunochromatography-based method for detecting enramycin residues in feed

To achieve rapid detection of enramycin in feed, we employed the competitive inhibition method to develop a colloidal gold immunochromatographic test strip based on the anti-enramycin A monoclonal antibody (anti-Er.A-mAb). Colloidal gold probes were prepared with a laboratory-prepared high-purity anti-Er.A-mAb. The effects of pH, antibody titer, and antigen concentration (test line) on the test strip performance were investigated. The colloidal gold test strip prepared with 8 μL potassium carbonate addition, 4 µg/mL antibody, 1.0 mg/mL antigen (test line), and 3 μL gold-labeled antibody showed acceptable specificity and a low limit of detection. The test strip showed the detection limit of 25 ng/mL for enramycin A, with a linear range of 25-300 ng/mL. The experiments on the feed with positive sample addition proved that the test strip had good repeatability and was more sensitive than high-performance liquid chromatography, being applicable for the rapid detection of enramycin in large batches of feed samples.

The Impact of Silica Addition on Floc Volume Density in the Biofloc System for Juvenile Red Tilapia (Oreochromis niloticus)

Biofloc technology is a solution to the problem of waste management in aquaculture. Biofloc systems are beneficial because they not only reduce inorganic nitrogen waste from feed residues and fish excrement but also produce flocs. The aim of this research is to determine the effect of silica addition on floc volume density in the biofloc system for juvenile red tilapia. The research was conducted from January to March 2024 at the Center of Excellence in Science and Technology (PUI-PT) for Functional Nano Powder at Padjadjaran University. This study uses a Completely Randomized Design (CRD) experimental method with four treatments and three replications. The treatments used were (A) control, (B) 15 ppm silica, (C) 20 ppm silica, and (D) 25 ppm silica. The study used cylindrical containers with a capacity of 19 liters filled with 15 liters of water, a stocking density of 10 juvenile red tilapia per cylinder, with the juvenile measuring 3-4 cm and weighing 3-4 g. The parameters observed were floc volume density and water quality. The data on floc volume density and water quality were analyzed descriptively. The results showed that the addition of 25 ppm silica in the biofloc system was the best treatment for floc volume density. This was evidenced by the results in treatment D (25 ppm) achieving a floc volume density of 26.5 ml/L, which falls into the dense category. Therefore, the addition of silica concentration in the biofloc system can increase floc volume density.

Efficiency of using the "Meadow of Health" feed table cover

The results of using the polymer cover of the feed table "Meadow of Health" in comparison with the concrete feed table are presented. The research was carried out in the conditions of the dairy livestock complex of the Kirov region on milk cows of the Black-and-White Holstein breed during the lactation period. Concrete feed tables for cows during the lactation stabilization period at the time of carrying out had average wear, while the cover of the feed table "Meadow of Health" remained smooth and even for 6 months. The study of microbial contamination of feed table covers showed, that already one month after the beginning of the experiment, the number of microbial cells was 54.7% lower (p < 0.05) in the 1st experimental group, where the "Meadow of Health" cover was used, compared to the concrete table. The amount of uneaten feed residues on the feed tables with the "Meadow of Health" cover was less in comparison with the concrete feed table (p < 0.05), which indicates better palatability of forage mixture from this cover. When transferring cows from polymer cover to concrete from the 2nd month of lactation, a decrease in average daily milk yields by 0.9 kg was observed. By the 4th month of lactation, the reduction in milk yield was 4.8 kg of milk per head per day. When transferring cows from a concrete feed table to a polymer one, there was an increase in milk productivity by 0.4–1.3 kg per head/day. In the first 3 months, the consumption of feed from a polymer-coated table increased by 2.3–5.6% compared to the concrete one. During 5 months of lactation, cows from the cover of the feed table "Meadow of Health" ate feed by 6.8–8.6% more than from concrete, which led to greater productivity of the animals during the analyzed period. The use of the polymer cover of the feed table "Meadow of Health" allowed to get additional profit in the amount of 20,460 rubles and recoup the costs of its installation already on the 39th day of use.

Replacing soybean meal with lesser mealworm Alphitobius diaperinus improves broiler productive performances, haematology, intestinal morphology and meat quality

Abstract Insects are becoming more popular as a soybean meal substitute because they can be raised sustainably on food and feed residues, which improves farming efficiency, sustainability, and animal welfare. In the current study, we replaced 4, 8 and 12% SBM with lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) larvae and determined its effects on productive performances, blood parameters, gut morphometry and meat quality of the broiler chickens. We also compared commercially available black soldier fly Hermetia illucens (Diptera: Stratiomyidae) larvae. A total of 350 one-day-old broiler chicks (Ross 308) were randomly assigned to seven isocaloric and isonitrogenous diets (5 pens/treatment and 10 birds/pen). The results found that 12% A. diaperinus and H. illucens diets significantly () increased the live weight (LW) and average daily weight gain (ADG) while improving feed conversion ratio (FCR) in the broilers. Daily feed intake (DFI) did not show a significant difference () among all the dietary treatments. Haematology and serum biochemistry traits were significantly () improved except MCHC, monocytes, Eosinophils (EO), albumin and creatinine. The 12% A. diaperinus and H. illucens diets showed the highest villus height (Vh) and the lowest crypt depth than other diets. The meat quality traits were statistically () improved except meat pH, drip loss and shear force (). The results of the study highlight the importance of substituting SBM up to 12% with either A. diaperinus or H. illucens larvae, which improves the productive performances, blood parameters, gut morphometry and meat quality of broilers and thus can be incorporated as an alternative protein source in poultry feed.

Effects of aflatoxin B1 on metabolism- and immunity-related gene expression in Hermetia illucens L. (Diptera: Stratiomyidae)

Contamination of food products with mycotoxins such as aflatoxin B1 (AFB1) poses a severe risk to human health. Larvae of the black soldier fly (BSFL), Hermetia illucens (Diptera: Stratiomyidae), can successfully metabolize AFB1 without any negative consequences on their survival or growth. Organic waste streams contaminated with mycotoxins can be upcycled into protein-rich BSFL as an alternative feed for livestock and the left-over feed residue into nutrient-rich crop fertilizers. However, the underlying mechanisms that allow BSFL to metabolize AFB1 are unknown. In this study, five-day-old BSFL were fed with either a control or an AFB1-spiked (20 μg/kg) diet to elucidate the underlying mechanisms. Larval samples were collected at three timepoints (6 h, 24 h and 72 h) and subjected to RNA-Seq analysis to determine gene expression patterns. Provision of an AFB1-spiked diet resulted in an up-regulation of 357 and a down-regulation of 929 unique genes. Upregulated genes include multiple genes involved in AFB1 metabolism in other (insect) species. Downregulated genes were generally involved in the insects' growth, development, and immunity. BSFL possesses a diverse genetic arsenal that encodes for enzymes capable of metabolizing AFB1 without trade-offs on larval survival. In conclusion, the adverse impact of AFB1 exposure on immunity-related processes is observed in the transcriptomic response, and is indicative of a trade-off between detoxification and immune responses.

Water quality monitoring with an early warning system for enhancing the shrimp aquaculture production

Aquaculture provided 43% of the aquatic animal food consumed by humans in 2007, and it is anticipated to expand even more to meet the increasing future demand. Marine Science Techno Park (MSTP) is one of the Techno Parks in Indonesia and is located in Teluk Awur, Jepara. MSTP has an intensive system of Vannamei shrimp farming activities. The challenge that has been faced annually is the organic matter of the remaining shrimp feed that accumulates in the waters can cause a decrease in pond water quality. Ammonia-N anions derived from the decomposition of feed residues can cause toxins in shrimp culture ponds which can further interfere with shrimp survival. The objective of this study is to design a self-controlled water quality monitoring system that is equipped with an early warning system based on internet of things (IoT) and to implement the design for analyzing the water quality including dissolved oxygen (DO), pH, and temperature in the Vannamei farming pond of MSTP UNDIP through integrating IoT which is equipped with an early warning system. If the water quality reaches a threshold value, the monitoring system will send a sound signal to the buzzer followed by sending an alert notification in real-time conditions automatically to a smartphone.

Optimizing Sustainability: Pemula Peternak Puyuh dan Inovasi Kompos Limbah Organik sebagai Pemacu Pertanian Berkelanjutan

The involvement of young quail farmers is essential for understanding their role in adopting practices such as composting quail manure. The participants in this Community Service activity are the younger generation, specifically the children of quail farmers in Grinting Village. This approach aims to cultivate environmentally conscious and committed young quail farmers dedicated to sustainable farming practices. The Community Service method is divided into three components: (1) Providing Insight on the Importance of Quail Livestock Waste Management for Young Beginner Farmers, (2) Composting Practice with Quail Manure, and (3) Completing the Compost Understanding Questionnaire. During this Community Service activity, straightforward yet easily comprehensible discussion material was presented on the preparation of young quail farmers. Their practical involvement in quail farming needs enhancement to foster a more comprehensive understanding. The primary focus is on establishing a fundamental understanding of quail farming as an economic potential, with a particular emphasis on ethical responsibility regarding livestock waste management, encompassing both manure and feed residues. The severe consequences of ineffective waste management are elucidated, including the impact of pollution on water and air, as well as associated health risks and economic losses. Awareness of the importance of adhering to environmental regulations is emphasized for the sustainability and safety of livestock. Technical solutions, such as optimal cage design and composting, are recommended to achieve a clean environment and enhance livestock productivity. Active participation of prospective farmers in waste management practices and collaboration with fellow farmers are strongly encouraged. Additional resources, in the form of guides and visual materials, are provided to support the implementation of these concepts

Extention and interlaboratory comparison of an LC-MS/MS multi-class method for the determination of 15 different classes of veterinary drug residues in milk and poultry feed

An HPLC-MS/MS multi-class method for quantitation of 15 different classes of veterinary drug residues (>140 analytes) in milk and poultry feed was developed and validated. Accuracy criteria for routine laboratories were met for the majority of analytes, > 83 % in milk and between 50 and 60 % in chicken feed, with an apparent recovery of 60–140 %. Extraction efficiency criteria were met for >95 % of the analytes for milk and > 80 % for chicken feed. Intermediate precision meets the SANTE criterion of RSD < 20 % for 80–90 % of the analytes in both matrices. For all analytes with an existing MRL in milk, the LOQ was below the related MRL. Twenty-nine samples of commercial milk and chicken feed were analyzed within the interlaboratory comparison. No residues of veterinary drugs were found in the milk samples. However, the feed samples exhibited high levels of nicarbazin, salinomycin, and decoquinate.

Conjugation of tetracycline with carrier proteins and production of its polyclonal antibody for the development of rapid test

Tetracycline (TC) is a broad-spectrum antibiotic used in the treatment of several infectious diseases. The excessive use of tetracycline antibiotics, especially tetracyclines in livestock, has raised concerns about residues of tetracycline antibiotics in food that may affect consumers' health. It is necessary to develop a rapid test strip for detecting tetracycline antibiotic residues in food and animal feed. This paper presents the suitable conditions for the conjugation of tetracycline to BSA or KLH: molar ratio of TC:BSA/KLH was 25:1, formaldehyde concentration of 5 %, temperature of 4oC and reaction time of 4 hours. The KLH-TC conjugate was used to successfully raise polyclonal antibodies against TC in rabbits. The anti-TC polyclonal antibodies were purified and could be used for the development of the lateral flow immunoassay test strip for the detection of tetracycline.

Biogas Dynamics and Microbial Composition Employing Different Inocula and Substrates in Continuous Stirred-Tank Reactors

The current investigation delved into the utilization of cattle and municipal sanitary inocula for anaerobic digestion of poultry wastes, addressing a crucial and pragmatic challenge in waste management. The emphasis on poultry waste is pertinent due to its well-documented impediments in anaerobic digestion, attributed to heightened levels of ammonia and volatile fatty acids (VFAs). The strategic selection of cattle and municipal sanitary inocula suggests an approach aimed at bolstering the anaerobic digestion process. In this study, we evaluated the use of cattle and municipal sanitary inocula for the anaerobic digestion of various poultry wastes, which is often challenged by high levels of ammonia and volatile fatty acids (VFAs). The substrates tested included belt waste (Poultry A), poultry litter plus feed residues (Poultry B), tray hatchery ©, and stillage. These substrates were processed in two continuous stirred tank reactors (CSTRs), R-1 (with antibiotic monensin) and R-2 (without monensin). Initially, both reactors operated with the same hydraulic retention time (HRT), using a substrate ratio of stillage: belt: tray hatchery (S:B:T) of 70:15:15. On the 41st day, the HRT was adjusted to 20 days, and the substrate ratio was changed to S:A:T 70:40:40. The specific methane yield for R-1 started at 10.768 L g−1 COD, but decreased to 2.65 L g−1 COD by the end of the experiment. For R-2, the specific methane yield varied between 0.45 L g−1 COD and 0.243 L g−1 COD. Microbial composition in the reactors changed over time. In R-1, bacteroides were consistently dominant, while firmicutes were less abundant compared to R-2. Proteobacteria were initially low in abundance, but spirochetes were found in both reactors throughout the experiment. The study concluded that Poultry B substrates, due to their rich nutrient and trace element composition, are suitable for biogas plants. Municipal sanitary inocula also showed promise due to their resilience in high ammonia concentrations. Further research into biofilm interactions is recommended to better understand microbial responses to high ammonia concentrations, which can lead to propionate production in anaerobic digestion (AD).

Anti-obesity activity of Erythrina subumbrans (Hassk.) Merr leaves extract in high fructose-induced obesity in Wistar rats

Introduction: Obesity is known to lead to the development of metabolic disorders and Erythrina subumbrans (Hassk.) Merr has shown the potential in alleviating some diseases. This study aimed to assess the anti-obesity activity of the ethanol extract of E. subumbrans leaves (EES) in obese Wistar albino rats. Methods: The rats were randomly assigned to eight groups (n = 5): normal group (no obesity inducement, treated with standard feed); high fructose (60%)-induced obesity (obesity induced, no treatment); control-1 group (obesity-induced, treated with orlistat 10.8 mg/kg BW); control-2 group (obesity-induced, treated with Isprinol 45 mg/kg); control-3 group (obesity-induced, treated with metformin 45 mg/kg); three test groups (obesity-induced, treated with EES 100, 200, and 400 mg/kg, respectively). The obesity inducement was conducted for 7 weeks. The rats were observed for body weight (BW), feed residue, feces index, triglycerides, organ index, and leucocyte profile. Results: The screening for secondary metabolites in EES revealed the presence of flavonoids, alkaloids, saponins, polyphenols, and tannins. EES decreased the percentage of BW gain, appetite, organ index (spleen), total fat, triglycerides, and leucocyte profile of the rats. Conclusion: The ethanol extract of the leaves of E. subumbrans has the potential to be developed as an anti-obesity agent, although the molecular mechanism of its pathway modulation is still unknown.

By-Products of the Babassu Agribusiness for Ruminant Diets.

The use of babassu agro-industrial residues in animal feed, in addition to being an economic option of great importance in reducing the environmental impact in regions of the Brazilian Cerrado, provides the production of good quality foods of animal origin due to its nutritional characteristics. However, information related to the nutritional components of babassu by-industrial residues has not yet been sufficiently explored. The aim of this study was to evaluate the nutritional potential of some by-products from the babassu production chain through chemical composition and in situ degradability analyses. The experiment was conducted in a completely randomized experimental design, with 4 by-products from babassu processing (cake, greasy, fine flour, and 95 µm flour) and 5 replications. The by-products differ in terms of chemical composition, except for the hemicellulose content. For the degradability of fraction "a" of dry matter, it presented a higher percentage for 95 µm flour. Fine flour and 95 µm flour presented the highest fractions "b" and "c," potential, and effective degradability of dry matter. For the degradation of crude protein, the highest percentages of potential and effective degradability were observed for greasy and 95 µm flour. The highest standardized potentially degradable fraction and the highest passage rate were obtained by 95 µm flour, which also showed greater degradation for dry matter, crude protein, and neutral detergent fiber. Among the by-products studied, the babassu cake has superior chemical composition; however, the 95 µm flour presented nutritional value and satisfactory rumen degradation to be used as an additive or in partial replacement of traditional concentrates.

Assessing the performance of various sorbents in micro-solid phase extraction cartridges for pesticide residue analysis in feed.

Newly designed micro-solid phase extraction cartridges are now available, reflecting the increasing shift towards laboratory automation, especially in the clean-up step for the analysis of pesticide residues in food and feed. In the present study, the introduction of different sorbents on the newly designed PAL µSPE CTC cartridges was investigated for the removal of matrix interferents and the recovery of pesticides. Eight cartridges containing different sorbent combinations and different amounts were used including EMR-lipid (not activated), Z-sep, chitin, C18, PSA, and GCB. The evaluation of co-extractive removal for each cartridge showed that the optimal choice for removing fatty acids was the cartridges containing PSA and Z-sep as clean-up sorbents. However, the presence of C18 and EMR-lipid was still required for the removal of sterols and tocopherols. Two grams of sample, fish feed (FF) and rapeseed cake (RSC) were extracted using QuEChERS citrate buffer, followed by a freeze-out step. The recoveries and repeatability of QuEChERS using µ-SPE clean-up were evaluated for 216 pesticide residues (112 compounds analyzed by GC-MS/MS and 143 compounds by LC-MS/MS, from which 39 compounds were analyzed using both techniques). The best results, with recovery between 70 and 120% and RSD <20%, were achieved when FF samples were cleaned-up with 15 mg EMR-lipid and 20 mg MgSO4. This was achieved for 94% of GC-amenable compounds and 86% of LC-amenable compounds. In the case of RSC, the best results were seen when samples were cleaned-up with the cartridge containing only 20 mg Z-sep and 20 mg MgSO4. This was achieved for 88% of GC-amenable compounds and 90% of LC-amenable compounds. Although these cartridges yielded optimal results in terms of recovery, their use could require more instrument maintenance, especially for GC-MS/MS, due to the lower removal of co-extractives.

Effect of the Meal Interval Setting of an Automated Concentrate Feeding System on Feed Intake and Feeding Behavior in Fattening Hanwoo Steers.

An automatic concentrate feeding system (ACFS) divides the day into several intervals, allowing cattle to consume a predetermined amount of concentrate mix per interval. This study investigated the impact of changing these intervals (four vs. six) in an ACFS on its precision in monitoring the feed intake and feeding behavior of fattening Hanwoo steers. The experiment, involving 29 fattening Hanwoo steers (688 ± 43.3 kg of body weight, 24 months old), employed a switchback design with two interval settings: four and six per day. Both individual forage and concentrate intakes and feeding behaviors were automatically recorded; however, the ACFS measured feed supply, not actual intake. The precision of the ACFS's intake recordings was tested by manually assessing feed residuals per visit using video recordings. Although no difference was observed in the concentrate intake (p > 0.05), the six-interval setting reduced concentrate residuals by 0.2 kg per visit (p < 0.05). The increased interval setting also resulted in fewer visits for forage consumption and decreased forage and total dry matter intakes (p < 0.05). In conclusion, the increased interval setting for the ACFS reduced the visit frequency for forage consumption and actual forage consumption while improving the precision of the ACFS's intake recordings.

Regenerative agriculture through indigenous practice of using remains of livestock feed residues as a manure to boost groundnut productivity

Cow represents Mother Earth, as it is a source of goodness, its milk nourishes all creatures and dung is store house of millions of microorganisms’ acts as source of organic manure and her urine aids in decomposition of crop residues. It’s in practice for millennium but recently due to introduction of chemical fertilizer found to be disappearing in the farming community coupled with reduction in the livestock population. Application of manure prepared from livestock feed for four years resulted in mean groundnut pod yield of 1665 kg/ha as against application of FYM i.e., 1108 and control 818 kg/ha.

Integration of Copperas and &lt;i&gt;Moringa oleifera&lt;/i&gt; Seeds as Hybrid Coagulant for Turbidity and Ammonia Removal from Aquaculture Wastewater

The rapid development of the aquaculture industry has contributed to the high amount of nutrients in wastewater that subsequently led to eutrophication and deterioration of water quality. Aquaculture wastewater consists of uneaten fish feed, fecal and other excretion or residue of chemicals used. Thus, this study aimed to evaluate the performance of hybrid coagulants of Moringa oleifera (MO) and copperas for aquaculture wastewater treatment. In this present study, different formulations of MO and copperas were explored in the coagulation treatment of aquaculture wastewater using a jar test experiment. The FTIR and SEM analysis are used to determine the morphology and surface of MO. This study focuses on the effect of coagulant aids formulation, coagulant dosage, the effect of initial pH and coagulation time on turbidity and ammonia removal in the coagulation of aquaculture wastewater. The finding shows that the highest removal of turbidity and ammonia was obtained with the use of 80% MO and 20% copperas at the condition of initial pH of 6 at 20 min of coagulation time, with the highest percentage removal of 66% and 91%, respectively. The coagulation isotherm of hybrid coagulant 80:20 is well described with the Freundlich isotherm model which describes the surface heterogeneity.

Effectiveness of the improved method of lifelong diagnostics trichurosis of sheep

Today, the most accurate way to diagnose animal helminthiasis is laboratory research. Among them, lifelong coproovoscopy methods play a leading role in the study of the spread of gastrointestinal helminthiasis, particularly trichurosis, and in establishing the effectiveness of anthelmintics. Therefore, a promising research direction includes improving and testing modern methods of coproovoscopy for trichurosis in sheep. The work aimed to determine the diagnostic efficiency of the improved flotation method of coproovoscopy for trichurosis in sheep. In laboratory conditions, the effectiveness of well-known flotation methods and the proposed method of coproovoscopy in diagnosing sheep trichurosis were determined. The leading indicators of the effectiveness of laboratory methods were the indicator of the intensity of trichurous invasion, the coagulation ability of the flotation solution, and the crystallization time of a drop of flotation liquid on a glass slide. It was established that all methods of coproovoscopy used in the experiment have flotation properties relative to Trichuris eggs. However, the improved method of coproovoscopy showed a higher flotation ability concerning Trichuris ovis nematode eggs, where the proposed flotation liquid has pronounced coagulation properties concerning undigested feed residues, and also has a slow crystallization time of droplets on a slide, which is more than 50 min. The improved method uses a combined flotation liquid, whose main components were Ca(NO3)2, C12H22O11, and NaCl. The indicator of the intensity of trichuriasis invasion when using the improved method was 67.0 ± 17.5 eggs in 1 g of feces and exceeded the number of trichuriasis eggs detected by flotation methods using NaCl – by 2.3 times (P ˂ 0.001), C12H22O11 – by 2.1 times (P ˂ 0.001), NH4NO3 – 1.3 times (P ˂ 0.01), C12H22O11 + NaCl – 1.1 times. The obtained data on the effectiveness of the improved method of coproovoscopy allows us to recommend it for introduction into production for effective and accurate laboratory lifelong diagnosis of trichurosis in sheep.