Faster Wound Healing Research Articles

Bio-mediated synthesized zinc oxide coated on cotton fabric for antibacterial and wound healing application

Developing novel approaches for the treatment of fabric to inherit antibacterial properties is pursued with great interest considering the growing demand, intermittently creeping pandemic situations, and increased awareness of clean, healthy, and hygienic lifestyles. Herein, in the present work, we have developed bio-mediated zinc oxide nanoparticles (ZnO NPs) by treating Azadirachta indica (Neem) leaf extract with zinc acetate. Further, the ZnO NPs were coated on the cotton fabric using an environmentally friendly ultrasound-assisted coating method without the aid of binders. The as-synthesized ZnO NPs and ZnO-coated cotton fabric were investigated using XRD, FTIR, ATR-FTIR, SEM-EDAX, and TGA analysis. The antibacterial activity, wound healing, mechanical and wash durability properties of ZnO-coated cotton fabrics were also investigated. The results demonstrate that the ZnO-coated fabric exhibits significantly better antibacterial against E. coli and S. aureus bacteria. In addition, the in-vivo studies revealed that ZnO NPs coated cotton shows faster wound healing than the control. Moreover, the coated fabric shows good adherence of ZnO NPs owing to the chemical bonding between NPs and cotton fabric. This chemical bonding gives good washing durability for up to 20 washing cycles. These findings suggest the potential of ZnO-coated antibacterial wound dressings for biomedical and healthcare applications.

Amphiphilic Janus cotton gauze with enhanced moisture management and blood coagulation for rapid hemostasis and wound healing

Cotton gauze is commonly used in initial emergency care. However, its high hydrophilicity and limited clotting capacity can lead to the excessive absorption of blood, resulting in unnecessary blood loss. Herein, an amphiphilic Janus cotton gauze with excellent moisture management and enhanced blood coagulation has been developed via in situ generating bioactive glass (BG) onto the cotton gauze (CG), and then attaching cardanol (CA) onto one side of the BG-loaded CG (CG@BG) via click reaction. The Janus gauze (CA-CG@BG) has asymmetric wetting properties with a hydrophilic side (CA-CG@BGHL) and a hydrophobic side (HBCA-CG@BG). When applied to hemostatic, the porous and active BG on CA-CG@BGHL can rapidly initiate coagulation cascade to form a robust thrombus. CA on HBCA-CG@BG can entangled with each other, creating a hydrophobic barrier that prevents blood from flowing out. The hemostatic performance of CA-CG@BG is superior to that of CG in both rats and pigs. Interestingly, CA-CG@BG possesses unidirectional exudate removal. When applied to wound healing, the exudate can penetrate the hydrophobic HBCA-CG@BG to the hydrophilic CA-CG@BGHL, resulting in faster wound healing than CG. CA-CG@BG exhibits excellent cytocompatibility and hemocompatibility. This unique Janus dressing shows promise as a potential material for clinical applications in the future.

Assessment of Vacuum-Assisted Closure (VAC) Therapy in Orthopaedic Infections.

Introduction Chronic and infected orthopaedic wounds may result in profound morbidity, amputation, sepsis andeven death. It may need prolonged hospitalization and multiple surgical procedures for treatment. Vacuum-assisted dressing (VAD) is a comparatively newer modality for treating chronic non-healing wounds which helps in faster wound healing, decreases the frequency of dressing and reduces hospitalization time. The aim of our study is to evaluate the outcome of vacuum-assisted dressing (VAD) in the management of orthopaedic wounds. Materials and methods A case series including 20 patients with post-traumatic open fracture wounds, post-operative infected wounds and wounds with underlying chronic osteomyelitis were treated with VAD. Wound size was measured pre- and post-debridement and every five days, at the time of dressing change until the wound healed or grafted. The duration of wound healing or wound closure was measured and documented. Results Wound size decreased significantly and healthy granulation tissues were observed in all wounds after the application of vacuum-assisted dressing. Wound size decreased by an average of 22% after debridement and first vacuum-assisted dressing removal. Infection control was achieved in 18 out of 20 patients (90%) who had wounds closed either by secondary closure or by skin grafting. Conclusion We conclude that VAD is an efficient technique in the management of orthopaedic wounds, especially in the management of open fracture wounds but less effective in chronically infected wounds with underlying osteomyelitis.

Fabrication, Evaluation and In-vitro, In-vivo Efficacy of Electrospun Nanofiber Mats of Tinospora cordifolia Extract and Curcumin

The current research work highlights the fabrication and evaluation of nanofiber mats prepared using electrospinning technology containing Tinospora cordifolia extract and curcumin incorporated in chitosan polymer. The study of the prepared mats was carried out to optimize the formulations, to check in-vitro antibacterial activity, and to establish the effectiveness in diabetic wound healing by conducting an animal study. The nanofiber mats of curcumin and leaf extract of T. cordifolia using chitosan as a base polymer were prepared, optimized, and evaluated for antibacterial activity in-vitro, while their ability to enhance the wound healing process was checked using an animal model. The research parameters included the rate at which the wound contracted besides the time taken for epithelialization by using the wound healing model- excision technique. The results we found are encouraging for further studies. The nanofibers showed good antibacterial potential and faster healing of wounds. The research finished by developing an improved new formulation that effectively combats bacterial resistance, a common problem in the diabetic wound healing process. The mixture will not only fight resistant bacteria, but it will also help diabetic wounds heal faster because the T. cordifolia leaf extract is known to improve blood vessel growth and tissue remodeling.

Tailoring silk fibroin fibrous architecture by a high-yield electrospinning method for fast wound healing possibilities.

In this study, a novel array electrospinning collector was devised to generate two distinct regenerated silk fibroin (SF) fibrous membranes: ordered and disordered. Leveraging electrostatic forces during the electrospinning process allowed precise control over the orientation of SF fiber, resulting in the creation of membranes comprising both aligned and randomly arranged fiber layers. This innovative approach resulted in the development of large-area membranes featuring exceptional stability due to their alternating patterned structure, achievable through expansion using the collector, and improving the aligned fiber membrane mechanical properties. The study delved into exploring the potential of these membranes in augmenting wound healing efficiency. Conducting in vitro toxicity assays with adipose tissue-derived mesenchymal stem cells (AD-MSCs) and normal human dermal fibroblasts (NHDFs) confirmed the biocompatibility of the SF membranes. We use dual perspectives on exploring the effects of different conditioned mediums produced by cells and structural cues of materials on NHDFs migration. The nanofibers providing the microenvironment can directly guide NHDFs migration and also affect the AD-MSCs and NHDFs paracrine effects, which can improve the chemotaxis of NHDFs migration. The ordered membrane, in particular, exhibited pronounced effectiveness in guiding directional cell migration. This research underscores the revelation that customizable microenvironments facilitated by SF membranes optimize the paracrine products of mesenchymal stem cells and offer valuable physical cues, presenting novel prospects for enhancing wound healing efficiency.

Clinical comprehensive treatment protocol for managing diabetic foot ulcers: A retrospective cohort study.

Diabetic foot ulcers (DFUs) are a common complication of diabetes, often leading to severe infections, amputations, and reduced quality of life. The current standard treatment protocols for DFUs have limitations in promoting efficient wound healing and preventing complications. A comprehensive treatment approach targeting multiple aspects of wound care may offer improved outcomes for patients with DFUs. The hypothesis of this study is that a comprehensive treatment protocol for DFUs will result in faster wound healing, reduced amputation rates, and improved overall patient outcomes compared to standard treatment protocols. To compare the efficacy and safety of a comprehensive treatment protocol for DFUs with those of the standard treatment protocol. This retrospective study included 62 patients with DFUs, enrolled between January 2022 and January 2024, randomly assigned to the experimental (n = 32) or control (n = 30) group. The experimental group received a comprehensive treatment comprising blood circulation improvement, debridement, vacuum sealing drainage, recombinant human epidermal growth factor and anti-inflammatory dressing, and skin grafting. The control group received standard treatment, which included wound cleaning and dressing, antibiotics administration, and surgical debridement or amputation, if necessary. Time taken to reduce the white blood cell count, number of dressing changes, wound healing rate and time, and amputation rate were assessed. The experimental group exhibited significantly better outcomes than those of the control group in terms of the wound healing rate, wound healing time, and amputation rate. Additionally, the comprehensive treatment protocol was safe and well tolerated by the patients. Comprehensive treatment for DFUs is more effective than standard treatment, promoting granulation tissue growth, shortening hospitalization time, reducing pain and amputation rate, improving wound healing, and enhancing quality of life.

A closed-loop smart dressing based on microneedle and electrochemical micropump for early diagnosis and in-time therapy of chronic wound

Early diagnosis combined with in-time treatment is a promising strategy for chronic wound care, preventing wound deterioration and promoting healing. Different smart dressings have recently emerged to enable real-time wound status monitoring and offer active intervention. However, the drawbacks of existing smart dressings, including limited drug loading capacity and poor drug penetration into the deeper wound dermal and subcutaneous tissues, are yet to be addressed, resulting in unsatisfactory therapeutic effectiveness. In this study, a Microneedle-embedded closed-loop smart Dressing (MelD) is developed to simultaneously achieve chronic wound early diagnosis and in-time therapy. The simple impedance sensing electrodes are utilized to monitor wound impedance for chronic wound early diagnosis. Based on the sensing data, the drug delivery micropump in MelD is automatically activated for in-time therapy. By integrating micropump electrodes with a drug reservoir coupled-microneedle array, a practice solution for delivering enough drug fluid into deeper wound dermal/ subcutaneous tissues is developed. Compared to untreated diabetic mice, MelD, delivering sufficient growth factor to the wound and promoting drug entry into deep tissue layers, induces faster wound healing in treated mice. Meanwhile, MelD facilitates tissue formation and nascent collagen deposition. The enhanced drug storage dosage, improved drug penetration and closed-loop operation of Meld enable the first successful attempt of ensuring sufficient and efficient drug delivery by miniaturized all-in-one smart dressing, expanding the usage of closed-loop chronic wound care from laboratorial demonstration on limited kinds of superficial wounds to a wider range of wound types.

Reconstruction of the extensor tendon on the dorsal pedis with a chimeric skin-aponeurosis flap from the groin region

BackgroundComplex defects involving the extensor tendon on the dorsal pedis have been reconstructed using multiple procedures. Skin coverage and tendon transfers have also been performed. This study aimed to present our experience using a chimeric skin-aponeurosis flap for one-stage reconstruction of composite soft-tissue defects on the dorsal pedis. MethodsBetween May 2017 and September 2020, 12 patients with these defects received total treatment using a chimeric groin flap. Based on the superficial circumflex iliac vessels, the skin paddle resurfaced the cutaneous defect, and the vascularised external oblique aponeurosis was rolled to form a tendon-like structure to simultaneously replace the absent segment of the extensor tendons. A suitable “Y” bifurcation was dissected to enlarge the vessel diameter. Single-stage reconstruction was performed using a set of vascular anastomoses at the recipient site. ResultsFlap survival was achieved without significant complications. The hammertoe deformity was completely removed. The average dimension of the skin paddle was 8.0 × 13.0 cm (range, 6.5 × 11.0–10.0 × 14.0 cm), and the mean size of the aponeurosis was 8.0 × 4.0 cm (range, 6.0 × 3.0–10.0 × 5.0 cm). At the last follow-up visit, no morbidity was observed at the donor site. Natural shapes and walking functions were successfully achieved with a protective sensation. ConclusionThe chimeric groin flap with sheets of external oblique aponeurosis is a great candidate for one-stage reconstruction of composite soft tissue loss on the dorsal pedis. This approach provides cosmetic coverage, allowing faster wound healing and reduced tendon adhesions.

Microneedles for Enhanced Bacterial Pathogen Inactivation and Accelerated Wound Healing

AbstractBacterial wound infections are a significant socioeconomic concern in the modern healthcare industry owing to increased morbidity, prolonged hospital stay, and mortality. Bacterial infectious agents that colonize the wound bed develop biofilms, acting as a physical barrier that prevents the effective penetration of topical antimicrobials. Further, bacteria in such infectious wounds express a wide range of virulence factors promoting intercellular transmigration and host cell invasion complicating the treatment regimen. To address this need, a water‐dissolvable poly‐vinyl pyrrolidine (PVP), calcium peroxide (CPO) infused microneedle structure (denoted as PVP/CPO MN) for effective transdermal delivery of antimicrobial payload deep into the tissues is developed. Fluid exudate from the wound bed dissolves the PVP/CPO MN enabling the release of CPO deep into the infected wound bed. A slow catalytic decomposition of CPO results in the sustained release of reactive oxygen species (ROS) deep within the infected wound inhibiting the inter‐ and intracellular pathogens. Here, a systematic study of microneedle fabrication and sterilization after complete packaging is conducted to ensure scalability and safe applicability while maintaining mechanical and antibacterial properties. In vitro, antibacterial efficacy of the microneedles is validated against two common wound pathogens, Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus). Moreover, the PVP/CPO MN exhibited significant efficacy in eradicating both extracellular and intracellular bacterial populations within an in vivo porcine wound model. Additionally, the microneedle technology facilitated a faster wound healing, with ≈30% increase compared to control and a 15% improvement over conventional silver dressing.

Diminished γδ T Cells during Murine Allergic Skin Inflammation Is Mediated by IL-4 Signaling in Keratinocytes.

Atopic dermatitis results in diminished barrier function and altered production of antimicrobial peptides. Dendritic epidermal T cells (DETCs) play an important role in the wound repair and inflammation process. Our previous work identified an IL-4-dependent loss of DETCs in Stat6VT mice and in the MC903-induced skin inflammation mouse model. However, the mechanisms through which IL-4 mediates the loss of DETCs are unclear. In this study, we show that IL-4Rα germline knockout mice (Il4ra-/-) have increased DETCs, faster wound healing, and increased epidermal differentiation complex gene and fibronectin expression. The absence of IL-4Rα minimized the MC903-induced loss of DETCs, and reciprocal bone marrow chimera experiments in Il4ra-/- and wild-type mice demonstrated structural nonhematopoietic IL-4-responsive cell-mediated DETC homeostasis. Skin keratinocyte-derived IL-15 decreased dramatically in the MC903 model, while injection of IL-15 rescued DETC loss by promoting DETC proliferation and limiting apoptosis. Conditional deletion of IL-4Rα from keratinocytes using Il4rafl/fl K14-Cre mice showed an increase of DETCs, increased IL-15 production, and diminished skin inflammation following wounding. These results suggest that IL-4-dependent effects on DETCs in allergic skin inflammation are mediated by the IL-4Rα receptor of keratinocytes.

Fabrication, Characterization, and Biological Evaluation of T. terrestris Incorporated Titanium-Doped ZnO/Cellulose Nanocomposite Films as a Therapeutic Hemostatic Scaffolds for Diabetic Wound Healing.

The advent of biobased materials exhibiting remarkable effectiveness and performance has ushered in a paradigm shift in the field of biomedical science. Polymers are often used in the medical sector, particularly in the regeneration of bones, tissues, and wounds. Fast wound healing and self-healing polymers created from sustainable surroundings are attractive alternatives to create demand for new pathways in polymer research. This study investigates the efficacy of a biowaste-derived polymer, which was extracted and supplemented with titanium-doped ZnO nanoparticles along with medication in the form of an extract to evaluate its effectiveness in promoting wound healing. The prepared materials were further characterized using X-ray diffraction (XRD), UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM), optical microscopy, atomic force microscopy (AFM), tensile, and its color parameters. In vitro studies on wound healing were also conducted. The results clearly showed that the produced substance possesses properties that are noteworthy for wound healing.

Deferoxamine topical cream superior to patch in rescuing radiation-induced fibrosis of unwounded and wounded skin.

Topical patch delivery of deferoxamine (DFO) has been studied as a treatment for this fibrotic transformation in irradiated tissue. Efficacy of a novel cream formulation of DFO was studied as a RIF therapeutic in unwounded and excisionally wounded irradiated skin. C57BL/6J mice underwent 30 Gy of radiation to the dorsum followed by 4 weeks of recovery. In a first experiment, mice were separated into six conditions: DFO 50 mg cream (D50), DFO 100 mg cream (D100), soluble DFO injections (DI), DFO 1 mg patch (DP), control cream (Vehicle), and irradiated untreated skin (IR). In a second experiment, excisional wounds were created on the irradiated dorsum of mice and then divided into four treatment groups: DFO 100 mg Cream (W-D100), DFO 1 mg patch (W-DP), control cream (W-Vehicle), and irradiated untreated wounds (W-IR). Laser Doppler perfusion scans, biomechanical testing, and histological analysis were performed. In irradiated skin, D100 improved perfusion compared to D50 or DP. Both D100 and DP enhanced dermal characteristics, including thickness, collagen density and 8-isoprostane staining compared to untreated irradiated skin. D100 outperformed DP in CD31 staining, indicating higher vascular density. Extracellular matrix features of D100 and DP resembled normal skin more closely than DI or control. In radiated excisional wounds, D100 facilitated faster wound healing and increased perfusion compared to DP. The 100 mg DFO cream formulation rescued RIF of unwounded irradiated skin and improved excisional wound healing in murine skin relative to patch delivery of DFO.

Antibacterial and self-healing sepiolite-based hybrid hydrogel for hemostasis and wound healing

The process of wound healing necessitates a specific environment, thus prompting extensive research into the utilization of hydrogels for this purpose. While numerous hydrogel structures have been investigated, the discovery of a self-healing hydrogel possessing favorable biocompatibility, exceptional mechanical properties, and effective hemostatic and antibacterial performance remains uncommon. In this work, a polyvinyl alcohol (PVA) hybrid hydrogel was meticulously designed through a simple reaction, wherein CuxO anchored sepiolite was incorporated into the hydrogel. The results indicate that introduction of sepiolite greatly improves the toughness, self-healing and adhesion properties of the PVA hydrogels. CuxO nanoparticles endow the hydrogels with excellent antibacterial performance towards Staphylococcus aureus and Escherichia coli. The application of hybrid hydrogels for fast hemostasis and wound healing are verified in vitro and in vivo with rat experiments. This work thereby demonstrates an effective strategy for designing biodegradable hemostatic and wound healing materials.

Laser hemorrhoidoplasty procedure versus harmonic scalpel hemorrhoidectomy: a comparative study for the treatment of grades III and IV hemorrhoids

Background Hemorrhoids are one of the most commonly presented diagnoses of colorectal diseases. This study aimed to evaluate and compare the outcomes of laser hemorrhoidoplasty (LHP) and harmonic scalpel hemorrhoidectomy (HSH) in the management of grades III and IV hemorrhoids, focusing on some postoperative complications. Patients and methods A randomized, controlled, clinical trial involving 34 patients with grades III and IV hemorrhoids was conducted at the General Surgery Department, Faculty of Medicine, Helwan University, from June 2022 to March 2023. Ethical considerations were adhered to, and patients provided informed consent. The groups underwent either LHP or HSH, and comprehensive patient information was collected, including demographics and preoperative assessments. Postoperative care and follow-up evaluations assessed outcomes such as pain, bleeding, discharge, wound healing, incontinence, recurrence, and stenosis. Results Demographic distribution was similar between the LHP and HSH groups, with comparable operative times. Postoperative bleeding did not significantly differ between groups. HSH was associated with more early discharge, longer wound healing times, and significantly higher postoperative pain levels up to the third week. No significant differences were observed in stenosis, incontinence, or recurrence between the two groups. Conclusion Both HSH and LHP techniques were found to be safe and effective for managing hemorrhoids. LHP demonstrated advantages, including lower postoperative pain levels, reduced seromucous discharge, and faster wound healing compared with HSH. These findings provide valuable insights for clinicians in optimizing patient care during the management of hemorrhoids.

Magnetic field application in bone tissue regeneration: issue current status and prospects for method development

Relevance. Magnets have long been used to treat various diseases, especially in inflammatory processes. According to existing historical data, magnetotherapy was already used in ancient times by the Chinese, Egyptians and Greeks. Different magnetic field strengths affect cells in different ways, with medium-strength magnetic fields being the most widely used. The review presents a brief history and current state of the issue of using a magnetic field in bone tissue regeneration. Modern knowledge about the mechanisms of physiological and reparative regeneration, restoration of bone tissue is clarified, and modern areas of bone tissue engineering are considered, taking into account the characteristics of microcirculation and the effect of a magnetic field on the physiology of bone tissue and reparative regeneration. One of the key findings of the review is that the magnetic field improves bone tissue repair by influencing the metabolic behavior of cells. Studies show that magnetotherapy promotes the activation of cellular processes, accelerates the formation of new bone tissue and improves its quality. It is also noted that the magnetic field has a positive effect on microcirculation, improving the blood supply to tissues and facilitating a better supply of nutrients to the site of injury. This contributes to faster wound healing and early rehabilitation of patients. Conclusion. Magnetotherapy is one of the effective physical and rehabilitation methods of treatment that will become increasingly important in modern medicine. However, further research is needed to better understand the mechanisms of action of a magnetic field on bone tissue and to determine the optimal parameters for its application.

Comparing Minimally Invasive Techniques to Traditional Surgery for Specific Tumor or Cancer Types: A Comprehensive Review

Surgical oncology encompasses the use of surgical interventions in the treatment of cancer. Its primary objective is the identification and removal of malignant tumors within the body. Surgeons specializing in this field are tasked with various responsibilities, including preventive measures, early detection of cancerous or pre-cancerous conditions, diagnosis, pre-operative preparation, tumor removal, staging of the disease, post-operative care, rehabilitation, surveillance, emergency interventions, and palliative treatments. The treatment of tumors often involves a combination of surgical procedures, chemotherapy, radiotherapy, and other supplementary therapies. Advancements in medical technology have led to less invasive surgical techniques, particularly in treating benign conditions, resulting in smaller incisions and reduced risks of complications compared to traditional open surgeries. Minimally invasive surgery (MIS) refers to procedures that minimize the size of surgical incisions, leading to decreased blood loss, faster wound healing, reduced pain and scarring, shorter hospital stays, lower infection risks, and fewer post-surgical complications. Consequently, many conditions that previously necessitated open surgery can now be managed through minimally invasive techniques. In contrast, traditional surgery has historically been the standard approach for cancer treatment, involving incisions to directly access and address the affected area. Skilled surgeons utilize their expertise and specialized tools to excise tumors, repair tissues, and conduct necessary procedures. The aim of this project is to evaluate and compare the efficacy of these surgical approaches in the treatment of specific types of tumors or cancers.

Flax seeds for human health and their benefits

Flaxseed (Linum Usitatissimum L) is celebrated for its abundance in Omega-3 polyunsaturated fatty acids, particularly alpha-linolenic acid, offering a myriad of health benefits. Through biochemical scrutiny, its bioactive lignans have been singled out as potentially advantageous for human health enhancement. Cultivated globally for its nutritional richness and therapeutic properties, flaxseed is utilized for both its fiber and oil content, correlating with a diminished risk of various conditions like cardiovascular disease, diabetes, cancer, osteoporosis, arthritis, and autoimmune and neurological disorders. Regular consumption has been associated with lowered blood pressure, improved lipid profiles, and enhanced insulin resistance, while its antioxidant properties promote healthier skin, faster wound healing, and alleviate menopausal symptoms such as constipation and mental fatigue. However, alongside its beneficial components, flaxseed contains compounds like phytic acid, cadmium, trypsin inhibitor, and cyclogenic glycosides (CGs), which may impede its health-promoting effects and reduce the absorption of critical nutrients. Available in three primary forms - flaxseed oil, ground flaxseed, and whole flaxseed - integrating it into one's diet could serve as a proactive measure against various health issues, underlining the plant's remarkable versatility and potential in bolstering overall well-being.

In vitro Wound Healing Effect of a Siddha Formulation: Gandhaga Thailam

In vitro Wound Healing Effect of a Siddha Formulation: Gandhaga Thailam

Synthesis of Injectable Thermosensitive Cinnamaldehyde-Loaded Chitosan Hydrogel for Antibacterial and Wound Dressing Application

The wound healing in the presence of bacterial infections is a complex process and prolonged challenge with severe implications for human health. To address this issue, injectable thermo-sensitive hydrogels as wound dressings have garnered considerable attention as a potential solution. In this study, we have developed an injectable and antibacterial thermosensitive hydrogel for wound healing, based on chitosan (CS) and β-glycerophosphate (β-GP) and modified with a range of cinnamaldehyde (CA). The CS/β-GP/CA gels demonstrate a phase transition at body temperatures, and exhibit good biocompatibility. Compared with the unmodified CS/β-GP hydrogels, incorporating CA can promote endothelial cell growth and the antibacterial property of the hydrogel system, which may lead to faster wound healing and tissue regeneration. Our findings suggest that the CS/β-GP/CA thermosensitive hydrogel emerges as a promising candidate for an injectable wound dressing in wound treatments.

STUDY THE ANTIMICROBIAL ACTIVITY OF TOPICAL GEL FORMULATION OF “ERYTHROMYCIN AND DEXAMETHASONE” IN VITRO & IN VIVO

The present study focused on antimicrobial activity of a combined topical veterinary medicinal formulation of erythromycin ethyl succinate with dexamethasone for treatment skin infections, otitis, ocular infections and infectious wounds. In vitro, gel formula result showed good antibacterial activity with high stability after storage at (30-40) °C for 6 months. In vivo, formulated gel has shown a significant effectiveness promoting faster wound healing in lab animals (albino mice) and that result combined with histopathological view. Gel formula acts as a scaffold biomaterial for wound closure besides, antibacterial effective role that represent a promising strategy to increase the effective of erythromycin as topical delivery drug. The present study suggests that erythromycin gel is stable, safe, provides remarkable antibacterial susceptibility, reduces pain and wound recovery