Faster Wound Healing Research Articles

Argon Plasma Jet-Treated Poly (Vinyl Alcohol)/Chitosan and PEG 400 Plus Mangifera indica Leaf Extract for Electrospun Nanofiber Membranes: In Vitro Study.

The wound-healing process can be disrupted at any stage due to various internal and external factors. The inflammatory stage of the process plays a vital role in determining the outcome of the wound. Prolonged inflammation due to bacterial infection can lead to tissue damage, slow healing, and complications. Wound dressings made using materials such as poly (vinyl alcohol) (PVA), chitosan (CS), and poly (ethylene glycol) (PEG) with Mangifera extract (ME) added can help reduce infection and inflammation, creating a conducive environment for faster healing. However, creating the electrospun membrane is challenging due to balancing various forces such as rheological behavior, conductivity, and surface tension. To improve the electrospinnability of the polymer solution, an atmospheric pressure plasma jet can induce chemistry in the solution and increase the polarity of the solvent. Thus, this research aims to investigate the effect of plasma treatment on PVA, CS, and PEG polymer solutions and fabricate ME wound dressing via electrospinning. The results indicated that increasing plasma treatment time increased the viscosity of the polymer solution, from 269 mPa∙to 331 mPa∙s after 60 min, and led to an increase in conductivity from 298 mS/cm to 330 mS/cm and an increase in nanofiber diameter from 90 ± 40 nm to 109 ± 49 nm. Incorporating 1% mangiferin extract into an electrospun nanofiber membrane has been found to increase the inhibition rates of Escherichia coli and Staphylococcus aureus by 29.2% and 61.2%, respectively. Additionally, the fiber diameter decreases when compared with the electrospun nanofiber membrane without ME. Our findings demonstrate that electrospun nanofiber membrane with ME has anti-infective properties and can promote faster wound healing.

Effect of Free-Range Chicken Eggs on Perineal Wound Healing among Postpartum Women

10percent of maternal mortality during the postpartum period are caused by infection due to bleeding from tears in the birth canal (perineal wound). The wound healing process is influenced by the intake of protein-rich nutrients. Free-range chicken eggs from are a high source of protein (Komala, 2021). This study aims to determine the effect of free-range chicken eggs on perineal wound healing among postpartum women. This was a pre-experimental study with intact group comparison. There were 32 samples who were selected using purposive sampling technique. The samples were assigned into the Intervention Group (administered with free-range chicken eggs) and the Control Group (were not administered with free-range chicken eggs). Data collection was performed through direct observation of perineal wounds using the REEDA scale score. Data were analyzed using the Fisher Exact statistical test. The results showed that there was a faster wound healing process among respondents who were administered with free-range chicken eggs (81.2percent) compare to those who were not administered with free-range chicken eggs (25percent) with a p value of 0.002 less than 0.05. It can be concluded that the administration of free-range chicken eggs chicken eggs had an effect on perineal wound healing among postpartum women. It is expected that postpartum women who experience perineal wound starting from the second grade will consume boiled free-range chicken eggs to speed up the wound healing process.

Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications

Triphala is one of the commonly used ayurvedic formulations for gastrointestinal disorders. In Ayurveda, Triphala water has been used to wash wounds as an antimicrobial agent for wound healing. In this study, the wound healing property of Triphala was further enhanced by mixing equal proportions of medicinal plants like Acacia catechu, Glycyrrhiza glabra Azadirachta indica, and Coscinium fenestratum. The polyherbal extract was prepared from the new polyherbal formulation using a methanol-chloroform solvent mixture and analysed for its quality and quantity of phytochemicals. Then, the polyherbal extract at 1%, 3%, and 5% concentration was mixed with 10% polycaprolactone (PCL) polymer and converted into poly-herbal extract-loaded nanofibers using an electrospinning technique. The prepared nanofibers were characterized by FTIR, XRD, FESEM, contact angle, and water absorption. Further, the biological properties like antimicrobial activity and biocompatibility were also analysed. Finally, the in-vivo wound healing efficiency was studied using an animal model. The FTIR analysis reveals the presence of unique phytochemicals of plant extracts. Further, the FESEM confirms the presence of the drug on the surface of nanofibers. From the XRD, the reduction in crystallinity was observed due to herbal drug incorporation. Further, contact angle, water absorption also proved the absorbing ability of polyherbal-loaded nanofibers. The polycaprolactone-polyherbal (PCL-PHF) formulation of 3% and 5% loaded nanofibers shows faster wound healing compared to the control and standard. In addition, the polyherbal drug-loaded polycaprolactone shows faster granulation tissue formation and collagen synthesis compared to the standard group. The antioxidant property of the patch protects the wound from lipid peroxides and myeloperoxidase. Overall, the antimicrobial, antioxidant, and anti-inflammatory property of polyherbal extract aids in faster wound healing. This finding suggests that the polyherbal drug-loaded polycaprolactone nanofibrous mats could be used as an effective candidate for the treatment of open wounds.

Molecular basis of FAAH-OUT-associated human pain insensitivity.

Chronic pain affects millions of people worldwide and new treatments are needed urgently. One way to identify novel analgesic strategies is to understand the biological dysfunctions that lead to human inherited pain insensitivity disorders. Here we report how the recently discovered brain and dorsal root ganglia-expressed FAAH-OUT long non-coding RNA (lncRNA) gene, which was found from studying a pain-insensitive patient with reduced anxiety and fast wound healing, regulates the adjacent key endocannabinoid system gene FAAH, which encodes the anandamide-degrading fatty acid amide hydrolase enzyme. We demonstrate that the disruption in FAAH-OUT lncRNA transcription leads to DNMT1-dependent DNA methylation within the FAAH promoter. In addition, FAAH-OUT contains a conserved regulatory element, FAAH-AMP, that acts as an enhancer for FAAH expression. Furthermore, using transcriptomic analyses in patient-derived cells we have uncovered a network of genes that are dysregulated from disruption of the FAAH-FAAH-OUT axis, thus providing a coherent mechanistic basis to understand the human phenotype observed. Given that FAAH is a potential target for the treatment of pain, anxiety, depression and other neurological disorders, this new understanding of the regulatory role of the FAAH-OUT gene provides a platform for the development of future gene and small molecule therapies.

Efficacy of customized corneal crosslinking versus standard corneal crosslinking in patients with progressive keratoconus (C-CROSS study): study protocol for a randomized controlled trial

BackgroundKeratoconus is a degenerative disorder of the cornea leading to a protrusion and thinning with loss of visual acuity. The only treatment to halt the progression is corneal crosslinking (CXL), which uses riboflavin and UV-A light to stiffen the cornea. Recent ultra-structural examinations show that the disease is regional and does not affect the entire cornea. Treating only the affected zone with CXL could be as good as the standard CXL, that treats the entire cornea.MethodsWe set up a multicentre non-inferiority randomized controlled clinical trial comparing standard CXL (sCXL) and customized CXL (cCXL). Patients between 16 and 45 years old with progressive keratoconus were included. Progression is based on one or more of the following changes within 12 months: 1 dioptre (D) increase in keratometry (Kmax, K1, K2); or 10% decrease of corneal thickness; or 1 D increase in myopia or refractive astigmatism, requiring corneal crosslinking.DiscussionThe goal of this study is to evaluate whether the effectiveness of cCXL is non-inferior to sCXL in terms of flattening of the cornea and halting keratoconus progression. Treating only the affected zone could be beneficial for minimalizing the risk of damaging surrounding tissues and faster wound healing. Recent non-randomized studies suggest that a customized crosslinking protocol based on the tomography of the patient’s cornea may stop the progression of keratoconus and result in flattening of the cornea.Trial registrationThis study was prospectively registered at ClinicalTrials.gov on August 31st, 2020, the identifier of the study is NCT04532788.

777 Food Swamps, Food Deserts, and Wound Healing in Burn Patients at an Urban Academic Center

Abstract Introduction Many victims of burn injury live in food deserts or food swamps. Food deserts are low-income areas with minimal or no access to nutritious foods while food swamps are areas with much greater access to unhealthy, high-calorie foods. We examined the relationship between residency in a food swamp or food desert by census tract and wound healing. Methods We performed a retrospective review of burn patients who underwent split-thickness skin grafting at an ABA verified urban academic burn center between September 2018 and August 2022. Inclusion criteria were burn area less than 20% total body surface area (TBSA), age ≥ 18, and single operation for split-thickness skin grafting. Patient residential locations were used in conjunction with Modified Retail Food Environment Index (mREFI) data to classify residency in food deserts and food swamps. The primary outcome was time to donor site healing. Time-to-event Cox proportional regression analysis was performed to evaluate risk factors for poor wound healing. Results A total of 238 patients were included in the study, of whom 110 (46.2%) were female and 128 (53.8%) were male. Mean age was 48, BMI was 28.5, TBSA 3.7, and average days to donor site healing was 26. In categorizing residency by mREFI data, 45 (18.9%) individuals had good access, 11 (4.6%) resided in food deserts, and 182 (76.5%) resided in food swamps. Although time to healing was longer on average in the food desert (25.8 days) and food swamp (26.4 days) groups compared to the group with good access to food within ½ mile (22.9 days), this difference was not statistically significant. Not having diabetes was found to be associated with shorter time to wound healing (HR=1.68, 95% CI [1.15, 2.47], p=0.007). Living in a good access area was associated with faster wound healing (compared to swamp: HR=1.23, 95% CI [0.86, 1.75], compare to desert: HR=1.06, 95% CI [0.51, 2.20]), however this association was not statistically significant. Conclusions Residency in a food swamp or food desert does not significantly influence time to healing in burn injured patients. However, not having diabetes was associated with a shorter time to wound healing. This is the first study to compare residency in food deserts or food swamps by census tract level data with time to wound healing in an urban burn population. Applicability of Research to Practice Wound healing is a complex and important process known to be affected by malnutrition. Our data contributes to the current body of literature on nutrition and wound healing.

507 Earlier Graft Takedown Using Autologous Keratinocyte Suspension in Conjunction with Split Thickness Skin Grafting for the Treatment of Deep 2nd and 3rd Degree Burns

Abstract Introduction Autologous split-thickness skin grafts (STSG) have been the gold standard concerning treatment of both full and partial thickness burns. However, new treatments are emerging such as spray-on autologous keratinocyte suspensions (AKS). AKS is composed of a patient’s own keratinocytes, fibroblasts, and melanocytes. STSG in conjunction with AKS has showed promising results for the treatment of deep partial and full thickness burns. The standard graft takedown with STSG has historically been on postoperative day (POD) 6. Once graft take down occurs, occupational therapy can begin along with cessation of continuous splinting leading to more convenience and comfortability for the patient. With AKS, we propose that faster wound healing allows graft take down to occur earlier on POD 4. Methods A retrospective chart review was conducted at TTUHSC’s UMC Burn Center looking at patients that underwent STSG with AKS for the treatment of their burn. Patients were divided based on graft takedown on POD 4 or 6. Factors such as time to complete wound closure and postoperative complications were analyzed. Results The average age and total burn surface area in both groups was 43 years old and 28%. In the POD 4 group (n=10) the average time to healing was 2 months while the POD 6 group (n=21) had an average healing time of 3.1 months. Graft take and percentage of patients with changes in postoperative mobility and hypertrophic scarring were the same across both groups (98%, 30%, and 50%, respectively). Conclusions While time to healing was slightly different, there was not a statistically significant difference between the two groups. From this study we can conclude that graft take down can occur earlier in patients treated with STSG in conjunction with AKS. This allows for earlier therapeutic intervention and more convenience for the patient. Applicability of Research to Practice • Graft takedown can occur earlier on postoperative day 4 instead of 6 in patients treated with split-thickness skin grafting in conjunction with autologous keratinocyte suspension. • Earlier graft takedown allows for earlier occupational therapy and reduces the need for extensive splinting which can be uncomfortable for the patient. • Graft takedown can occur earlier in those treated with split-thickness skin grafting in conjunction with autologous keratinocyte suspension due to the rapid healing the suspension offers.

An Antibacterial andAnti-Oxidative Hydrogel Dressing for Promoting Diabetic Wound Healing and Real-Time Monitoring Wound pH Conditions with a NIR Fluorescent Imaging System.

The design and synthesis of multifunctional chitosan hydrogels based on polymerized ionic liquid and a near-infrared (NIR) fluorescent probe (PIL-CS) is a promising strategy, which not only prevents the transition from acute to chronic wounds, but also provides prompt measures regarding microenvironmental alterations in chronic wounds. PIL-CS hydrogel can real-time visualize wound pH through in vivo NIR fluorescent imaging and also feature the pH-responsive sustained drug release, such as antioxidant, to eliminate reactive oxygen species (ROS) and to boost diabetic wound healing. PIL-CS hydrogel is specific, sensitive, stable, and reversible in response to pH changes at the wound site. It, therefore, enables real-time monitoring for a dynamic pH change in the microenvironment of irregular wounds. PIL-CS hydrogel is also designed to possess many merits including high water containment and swelling rate, good biocompatibility, electrical conductivity, antifreeze, tissue adhesion, hemostatic performance, and efficient antibacterial activity against MRSA. In vivo studies showed that PIL-CS hydrogel provided fast diabetic wound healing support, promoted vascular endothelial growth factor (VEGF) production, and reduced ROS and tumor necrosis factor (TNF-α) generation. The results support that the hydrogels coupled with NIR fluorescent probes can be an excellent diabetic wound dressing for enhancing and real-time monitoring skin restoration and regeneration.

Defective NCOA4-dependent ferroptosis in senescent fibroblasts retards diabetic wound healing

Cellular senescence describes a state of permanent proliferative arrest in cells. Studies have demonstrated that diabetes promotes the pathological accumulation of senescent cells, which in turn impairs cell movement and proliferation. Historically, senescence has been perceived to be a detrimental consequence of chronic wound healing. However, the underlying mechanism that causes senescent cells to remain in diabetic wounds is yet to be elucidated. Ferroptosis and ferritinophagy observed in diabetes are due to iron metabolism disorders, which are directly associated with the initiation and progression of diabetes. Herein, we reveal that senescent fibroblasts in diabetic wounds are resistant to ferroptosis and that impaired ferritinophagy may be a contributing cause. Further, the expression of NCOA4, a key factor that influences ferritinophagy, is decreased in both diabetic wound tissue and high glucose-induced senescent fibroblasts. Moreover, NCOA4 overexpression could render senescent fibroblasts more vulnerable to ferroptosis. A faster wound healing process was also linked to the induction of ferroptosis. Thus, resistance to ferroptosis impedes the removal of senescent fibroblasts; promoting ferritinophagy could reverse this process, which may have significant implications for the management of diabetic wounds.

A mesenchymal stem cell-derived nanovesicle-biopotentiated bovine serum albumin-bridged gelatin hydrogel for enhanced diabetic wound therapy

The application of gelatin hydrogels is hampered by their poor mechanical properties, which are unfavorable for debridement and diabetic wound healing. Herein, bovine serum albumin (BSA)-bridged gelatin hydrogels biopotentiated by bone marrow mesenchymal stem cell (BMSC)-derived nanovesicles (NV), named NV@BSA-GEL, were fabricated by reinforcement of gelatin hydrogels with genipin and BSA in a one-pot, low-cost green approach based on amide bonding-guided crosslinking. Genipin introduction endowed the crosslinking of gelatin hydrogels with weak mechanical properties that were reinforced by BSA bridging to realize suitable mechanical properties for wound dressing, including excellent flexibility and appropriate rigidity. The bioactivity of NV released by the hydrogels resulted in efficient ROS scavenging and favorable immunomodulatory activity as well as promoted cell proliferation and migration of fibroblasts and vascular endothelial cells. Proteomic analysis confirmed that NV harbors a variety of growth factors that can promote wound healing. In vivo, the NV@BSA-GEL hydrogel had superior wound healing effects compared to BSA-GEL, including faster wound healing, stimulation of angiogenesis, contributing to an anti-inflammatory extracellular matrix, and promoting skin maturation, with no systemic toxicity. Thus, the combined merits of NV@BSA-GEL suggest that it has high potential as a new hydrogel dressing for the safe and efficient treatment of diabetic wounds.

Design and characterization of adipose-derived mesenchymal stem cell loaded alginate/pullulan/hyaluronic acid hydrogel scaffold for wound healing applications

Recently, significant attention has been focused on the progression of skin equivalents to facilitate faster wound healing and thereby skin restoration. The main aim of this study was the design and characterization of a novel polysaccharide-based hydrogel scaffold by using alginate, pullulan, and hyaluronic acid polymers to provide an appropriate microenvironment to deliver Adipose-derived mesenchymal Stem Cells (ASC) in order to promote wound healing in an animal model. Characterization of synthesized hydrogel was done by using a field emission scanning electron microscope (FE-SEM), Fourier Transform-Infrared spectroscopy (FT-IR), and Differential Scanning Calorimetry (DSC). Also, contact angle analysis, the swelling and mechanical tests were performed. As a result of in vitro studies, cells can be attached, alive, and migrate through the prepared hydrogel scaffold. Finally, the therapeutic effect of the cell-seeded hydrogels was tested in the full-thickness animal wound model. Based on obtained results, the hydrogel-ASC treatment improved the healing process and accelerated wound closure.

MP63-03 EFFICACY AND SAFETY OF a NOVEL 450 NM BLUE DIODE LASER VERSUS PLASMAKINETIC ELECTROCAUTERY FOR THE TRANSURETHRAL RESECTION OF NON-MUSCLE INVASIVE BLADDER CANCER: THE PROTOCOL AND RESULT OF a MULTICENTRE RANDOMIZED CONTROLLED TRIAL

You have accessJournal of UrologyCME1 Apr 2023MP63-03 EFFICACY AND SAFETY OF a NOVEL 450 NM BLUE DIODE LASER VERSUS PLASMAKINETIC ELECTROCAUTERY FOR THE TRANSURETHRAL RESECTION OF NON-MUSCLE INVASIVE BLADDER CANCER: THE PROTOCOL AND RESULT OF a MULTICENTRE RANDOMIZED CONTROLLED TRIAL Kaijie Wu, Lianhua Zhang, Shuai Jiang, Tianhai Lin, YI Luo, and Dalin He Kaijie WuKaijie Wu More articles by this author , Lianhua ZhangLianhua Zhang More articles by this author , Shuai JiangShuai Jiang More articles by this author , Tianhai LinTianhai Lin More articles by this author , YI LuoYI Luo More articles by this author , and Dalin HeDalin He More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003321.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To be the first to apply a novel 450 nm blue diode laser in transurethral resection of bladder tumour (TURBt) to treat patients with non-muscle invasive bladder cancer (NMIBC) and evaluate its efficacy and safety during the preoperative period compared to the conventional plasmakinetic electrocautery. METHODS: Randomized controlled trials (RCTs) in five medical centres were conducted from October 2018 to December 2019. Patients with NMIBC were randomized to the blue laser or plasmakinetic electrocautery group for TURBt. As the first study to evaluate this novel blue laser device, the primary outcome was the effective resection rate of bladder tumours, including effective dissection and haemostasis. The secondary outcomes were the perioperative records, including operation time, postoperative indwelling catheter time, hospital stay length, blood loss, reoperation rate, wound healing and adverse events. RESULTS: A total of 174 patients were randomized to either the blue laser group (85 patients) or plasmakinetic electrocautery group (89 patients). There was no statistical significance in the clinical features of bladder tumours, including tumour site, number and maximum lesion size. Both the blue laser and plasmakinetic electrocautery could effectively dissect all visible bladder tumours. The operation time for patients in the blue laser group was longer (p=0.001), but their blood loss was less than that of patients in the control group (p=0.003). There were no differences in the postoperative indwelling catheter time, hospital stay length, reoperation rate or other adverse events. However, the patients undergoing TURBt with the blue laser showed a faster wound healing at 3 months after operation. CONCLUSIONS: The novel blue laser could be effectively and safely used for TURBt in patients with NMIBC, and this method was not inferior to plasmakinetic electrocautery during the perioperative period. However, TURBt with the blue laser may provide the benefit to reduce preoperative blood loss and accelerate postoperative wound healing. Moreover, longer follow-up to confirm recurrence-free survival benefit was required. Source of Funding: This study was fifinancially supported by the National Key Research and Development Program (2019YFC0121501) © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e871 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kaijie Wu More articles by this author Lianhua Zhang More articles by this author Shuai Jiang More articles by this author Tianhai Lin More articles by this author YI Luo More articles by this author Dalin He More articles by this author Expand All Advertisement PDF downloadLoading ...

COMPARATIVE STUDY OF PLACENTREX GEL DRESSING'S VS AMORPHOUS HYDROGEL WITH COLLOIDAL SILVER IN CHRONIC CLEAN WOUNDS

Background: The process of cutaneous wound healing is incredibly complex, dependent on an intricate interplay between number of highly regulated factors working in concert to restore injured skin towards repaired barrier function.Any surgical practice primarily depends upon the healing of wounds without serious complications and infection occurring ,It accounts for considerable patient morbidity, discomfort and prolonged hospitalization. Objective :To compare between the use of Placentrex gel dressingvs Amorphous Hydrogel with colloidal silver in Patients who having chronic clean wounds Methodology:This comparative study was conducted among 140 patients with chronic clean wounds came getting admitted in the department of General Surgery,AVMC with Informed consent were included in the study. Placentrex gel dressing and Amorphous Hydrogel with colloidal silver dressing's was done .Patients were monitored daily and Data was calculated and analysed byusing SPSS version 22.0 (IBM SPSS, US) software operating on windows 10 Result:Total of 140 patients included in the study and divided into two groups of 70 based on the type of dressings done for the Patients .Found signicant lower duration of the hospitalization and cost for the Patients whoreceived Placentrex gel compared to Amorohous hydrogel with colloidal silver .Number of dressings needed for Patients who received placentrex is less comparedwith Amorophous hydrogel with colloidal silver . The patients who given placentrex gel were found to have Signicantly fast wound healing 20 vs 30 days (P<0.005). Conclusion: Placentrex gel is more effective in wound healing than hydrogel and the duration of hospital stay is decreased for patients who have chronic wounds. This study results found that placentrex gel ismore effective against acute and chronic clean wounds.

Sustainable sepiolite-based composites for fast clotting and wound healing

Uncontrolled bleeding and bacterial coinfection are the major causes of death after an injury. Fast hemostatic capacity, good biocompatibility, and bacterial coinfection inhibition pose great challenges to hemostatic agent development. A prospective sepiolite/Ag nanoparticles (sepiolite@AgNPs) composite has been prepared by using natural clay sepiolite as template. A tail vein hemorrhage mouse model and a rabbit hemorrhage model were used to evaluate the hemostatic properties of the composite. The sepiolite@AgNPs composite can quickly absorb fluid to subsequently stop bleeding due to the natural fibrous crystal structure of sepiolite, and inhibit bacterial growth with the antibacterial ability of AgNPs. Compared with commercially-available zeolite material, the as-prepared composite exhibits competitive hemostatic properties without exothermic reaction in the rabbit model of femoral and carotid artery injury. The rapid hemostatic effect was due to the efficient absorption of erythrocyte and activation of the coagulation cascade factors and platelets. Besides, after heat-treatment, the composites can be recycled without significant reduction of hemostatic performance. Our results also prove that sepiolite@AgNPs nanocomposites can stimulate wound healing. The sustainability, lower-cost, higher bioavailability, and stronger hemostatic efficacy of sepiolite@AgNPs composite render these nanocomposites as more favorable hemostatic agents for hemostasis and wound healing.

Ischemia Impaired Wound Healing Model in the Rat—Demonstrating Its Ability to Test Proangiogenic Factors

Chronic wounds remain a serious clinical problem with insufficient therapeutic approaches. In this study we investigated the dose dependency of rhVEGF165 in fibrin sealant in both ischemic and non-ischemic excision wounds using our recently developed impaired-wound healing model. An abdominal flap was harvested from the rat with unilateral ligation of the epigastric bundle and consequent unilateral flap ischemia. Two excisional wounds were set in the ischemic and non-ischemic area. Wounds were treated with three different rhVEGF165 doses (10, 50 and 100 ng) mixed with fibrin or fibrin alone. Control animals received no therapy. Laser Doppler imaging (LDI) and immunohistochemistry were performed to verify ischemia and angiogenesis. Wound size was monitored with computed planimetric analysis. LDI revealed insufficient tissue perfusion in all groups. Planimetric analysis showed slower wound healing in the ischemic area in all groups. Wound healing was fastest with fibrin treatment-irrespective of tissue vitality. Lower dose VEGF (10 and 50 ng) led to faster wound healing compared to high-dose VEGF. Immunohistochemistry showed the highest vessel numbers in low-dose VEGF groups. In our previously established model, different rhVEGF165 treatments led to dose-dependent differences in angiogenesis and wound healing, but the fastest wound closure was achieved with fibrin matrix alone.

Formulation and Characterization of Polymeric Cross-Linked Hydrogel Patches for Topical Delivery of Antibiotic for Healing Wound Infections.

Wound healing faces significant challenges in clinical settings. It often contains a series of dynamic and complex physiological healing processes. Instead of creams, ointments and solutions, alternative treatment approaches are needed. The main objective of the study was to formulate bacitracin zinc-loaded topical patches as a new therapeutic agent for potential wound healing. A free radical polymerization technique was optimized for synthesis. Polyethylene glycol-8000 (PEG-8000) was chemically cross-linked with acrylic acid in aqueous medium, using Carbopol 934 as a permeation enhancer and tween 80 as surfactant. Ammonium persulfate and N,N'-Methylenebisacrylamide (MBA) were utilized as initiator and cross-linker. FTIR, DSC, TGA, and SEM were performed, and patches were evaluated for swelling dynamics, sol-gel analysis, in vitro drug release in various media. A Franz diffusion cell was used for the permeation study. Irritation and wound healing with the drug-loaded patches were also studied. The characterization studies confirmed the formation of a cross-linked hydrogel network. The highest swelling and drug release were observed in formulations containing highest Polyethylene glycol-8000 and lowest N,N'-Methylenebisacrylamide concentrations. The pH-sensitive behavior of patches was also confirmed as more swelling, drug release and drug permeation across skin were observed at pH 7.4. Fabricated patches showed no sign of irritation or erythema as evaluated by the Draize scale. Faster wound healing was also observed with fabricated patches compared to marketed formulations. Therefore, such a polymeric network can be a promising technology for speeding up wound healing and minor skin injuries through enhanced drug deposition.

Alpha-ketoglutaric acid based polymeric particles for cutaneous wound healing.

Metabolites are not only involved in energy pathways but can also act as signaling molecules. Herein, we demonstrate that polyesters of alpha-ketoglutararte (paKG) can be generated by reacting aKG with aliphatic diols of different lengths, which release aKG in a sustained manner. paKG polymer-based microparticles generated via emulsion-evaporation technique lead to faster keratinocyte wound closures in a scratch assay test. Moreover, paKG microparticles also led to faster wound healing responses in an excisional wound model in live mice. Overall, this study shows that paKG MPs that release aKG in a sustained manner can be used to develop regenerative therapeutic responses.

Reconstruction of a Traumatic Large Upper Eyelid Avulsion with Tarsoconjunctival Graft and Rotational Flap: A Surgeon’s Preference

Introduction: Large, full-thickness upper eyelid defects management presents a thorough and challenging approach due to the multilaminar character of the eyelid and its dynamic function. The Cutler-Beard flap method has been the mainstay management, but posterior lamellar graft usage with recent modifications may reduce complications. Case Presentation: A 43-year-old man presented to the emergency ward after a traffic accident left him with a full-thickness laceration on his left upper eyelid and an approximately 60% avulsion on the one-third medial of the eyelid. Traumatic ptosis was also found. After considering the large size of the affected area and the probability of faster wound healing, a free graft from the tarsus and conjunctival eyelid of his fellow eye was harvested to replace the posterior lamellar of the affected eye and closed the defect with a rotational skin flap from the remaining of the left upper eyelid. Conclusions: This technique could be one of the alternatives to the more common cutler-beard flap with mild complications. However, temporary visual obstruction cannot be avoided due to the traumatic ptosis complication of this patient.

Assessment of Wound Healing Following Collagen Urethral Stent Placement for Urethrotomy in Dogs

Background: The present study was undertaken to evaluate the efficiency of collagen urethral stents on wound healing post-urethrotomy in dogs and to assess the uptake of collagen urethral stents through urethrography, ultrasonography and hematobiochemical analysis. Methods: A total of 12 dogs presented to Madras Veterinary College teaching hospital with the history of urinary obstruction were chosen for the study and were randomly divided into 2 groups of 6 dogs in each. In Group I, dogs were treated with conventional urethrotomy procedure and in group II, animals were treated with urethrotomy with collagen urethral stent placement. Hematobiochemical, plain and contrast radiography, ultrasonograhy were performed in both the groups. Result: The study revealed that in comparison with Group I, Group II animals had a faster wound healing rates; reduction in urethral lumen diameter and urethral leakage post-operatively were not observed in both the groups. In the present study, ultrasound was used to identify and locate the calculi but also to check the collapse, migration and absorption of the collagen stent placed inside the urethra wherein no stent collapse or migration noticed. Partial collagen stent absorption was noticed on day 7 and a complete stent absorption was noticed on day 14. There was no significant difference in the pain scores between the groups indicating that the collagen stent does not elicit any extra pain as compared to normal urethrotomy procedure.

Effect of Yak Meat to the Daily Ration of Scalded Rats for Wound Healing.

Treatment of burn wound healing involves infection, nutrition, psychology and rehabilitation, and proper nutritional support can promote wound healing, enhance immune function and reduce the incidence of complications. This study aimed to investigate the effects of feed containing yak meat on scalded rats' body condition and wound healing. Adopting a two-factor factorial design, the growth performance, food intake, body weight, and Lee's index of rats were measured. The wound conditions of scalded rats with different feeds (basic, basic + yak meat, and basic + yellow beef) were observed at different periods, and their wounds' hematoxylin and eosin (H&E) staining states were detected. The proliferating cell nuclear antigen (PCNA)-positive cells and apoptosis were analyzed to evaluate the effects of feed on the wound healing of scalded rats. The feed intake was the highest in the yellow beef feed group and the lowest in the yak meat feed group. The body weight was the highest in the yak meat feed group and the lowest in the yellow beef feed group. Furthermore, 45 days after scalding, the obesity index in the yak beef feed group was the closest to that of the rats before scalding. The wound recovery of the rats in the yak meat feed group was the best at 30 days, and the H&E staining results also proved that the recovery effect of the scalded rats in the yak meat feed group was better than other two groups. According to the results of PCNA and apoptosis, the yak meat feed group had lower positive cell rate and faster wound healing. The rats in the yak meat feed group recovered better than those in the other groups, and the yak beef feed had the best effect on the wound healing of the scalded rats.