The purpose of the article is to highlight the main milestones in the life and creative path of the famous scientist in the field of animal husbandry, Doctor of Biological Sciences, Professor I. V. Smirnov, his contribution to the formation of the sectorial experimental work and the spread of higher professional education. Research methods – general scientific (analysis, synthesis, classification), special historical (problem-chronological, comparative-historical, biographical), source studies. The scientific novelty of the article lies in the detailed study of the Terezyno period of I. V. Smirnov's activities, during which the new technological solutions were established and implemented contributing to the development of the theory and methodology of artificial insemination of farm animals. It is proved that on the basis of the Kiev Experimental Station of Animal Husbandry "Terezyno" the scientist implemented the concept of the development of higher professional education, based on the combination of the educational process with long-term industrial practice; laid the foundations for international cooperation in the field of reproduction biotechnology in animal husbandry. The main components of the I. V. Smirnov's creativity are highlighted, namely: methods of sperm long-term storage, technologies and techniques of artificial insemination, biology and physiology of reproduction of farm animals, etc. The researcher developed the theory of anabiosis, the theory of cold sperm shock, established the methods for mammalian sperm diluting and evaluating. He updated research on the use of blood groups for testing the lineage of animals and artificially regulating the sex of the offspring. It is shown that the method of mammalian sperm long-term storage formed the theoretical basis for the reorganization of domestic breeding on the basis of the widespread introduction of artificial insemination, contributed to a fast transformation of the gene pool of farm animals due to the involvement of genetic resources of foreign breeding in the selection process. It has been proved that thanks to the I. V. Smirnov's creative initiatives, the foundations have been laid for the development of a new direction in the science of the reproduction of organic species – reproductive biotechnology of farm animals.