Family Leguminosae Research Articles

In vitro and In vivo Evaluation of Fungicides, against Colletotrichum lindemuthianum Causing Anthracnose of Mungbean

Mungbean [Vigna radiata (L.) Wilczek] is a popular pulse crop, second only to chickpea and pigeon pea, and is grown in many parts of the world. Mungbean belongs to family Leguminosae. In the suitable condition bean is attacked by various diseases. Out of which, anthracnose caused by seed borne pathogen Colletotrichum lindemuthianum is an important fungal disease and major limiting factor for yield loss. Realizing the potentiality of the disease in causing economic losses, the different fungicides are tested against Colletotrichum lindemuthianum among the fungicides Difenoconazole 25% EC, Carbendazim 50% WP, Metiram 70% WG, Propiconazole 25% EC, Pyraclostrobin 20% WP Propineb 70% WP, Carbendazim 12% WP+ Mancozeb 63% WP, Hexaconazole 5% EC respectively.

Hepatoprotective Effect of Millettia dielsiana: In Vitro and In Silico Study.

In silico docking studies of 50 selected compounds from Millettia dielsiana Harms ex Diels (family Leguminosae) were docked into the binding pocket of the PI3K/mTOR protein. In there, compounds trans-3-O-p-hydroxycinnamoyl ursolic acid (1) and 5,7,4'-trihydroxyisoflavone 7-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (2) are predicted to be very promising inhibitors against PI3K/mTOR. They direct their cytotoxic activity against Hepatocellular carcinoma with binding affinity (BA) values, the pulling work spent to the co-crystallized ligand from the binding site of PI3K/mTOR (W and Fmax), and the non-equilibrium binding free energy (∆GneqJar) as BA values = -9.237 and -9.083 kcal/mol, W = 83.5 ± 10.6 kcal/mol with Fmax = 336.2 ± 45.3 pN and 126.6 ± 21.7 kcal/mol with Fmax = 430.3 ± 84.0 pN, and ∆GneqJar = -69.86074 and -101.2317 kcal/mol, respectively. In molecular dynamic simulation, the RMSD value of the PI3K/mTOR complex with compounds (1 and 2) was in the range of 0.3 nm to the end of the simulation. Therefore, the compounds (1 and 2) are predicted to be very promising inhibitors against PI3K/mTOR. The crude extract, ethyl acetate fraction and compounds (1 and 2) from Millettia dielsiana exhibited moderate to potent in vitro cytotoxicity on Hepatocellular carcinoma cell line with IC50 values of 81.2 µg/mL, 60.4 µg/mL, 23.1 μM, and 16.3 μM, respectively, and showed relatively potent to potent in vitro antioxidant activity on mouse hepatocytes with ED50 values of 24.4 µg/mL, 19.3 µg/mL, 30.7 μM, and 20.5 μM, respectively. In conclusion, Millettia dielsiana and compounds (1 and 2) are predicted to have very promising cytotoxic activity against Hepatocellular carcinoma and have a hepatoprotective effect.

Fenugreek (Trigonella foenum graecum) gum: A functional ingredient with promising properties and applications in food and pharmaceuticals—A review

AbstractFenugreek (Trigonella foenum graecum) is an annual plant belonging to the family Leguminosae or Fabaceae. The seeds of fenugreek contain 26.8% soluble fiber chemically identified as galactomannans with properties similar to soluble fiber of guar seeds and psyllium husk and so on. Galactomannans are biopolymers consisting of linear core poly (1,4)‐β‐D‐mannan backbone having varying degrees of D‐galactosyl substituents attached by 1,6‐glycosidic linkages. Different galactomannans derived from various legumes usually differ in molecular weight, ratio of mannose to galactose, and the mode and place of linkages of galactose moieties to the mannan backbone. Fenugreek gum has a galactose to mannose ratio of 1:1, showing the highest amount of galactose resulting in its highest water solubility compared with guar and locust bean gum. It suggested that the galactose and mannose moieties are linked uniformly in fenugreek gum resulting in maximum hydration and solubility. This M/G ratio affects the physicochemical properties of galactomannans and is inversely related to the solubility of gum. Galactomannans sterically stabilize the emulsions against flocculation and coalescence by getting adsorbed onto oil droplets. In addition to this, galactomannans are all found to exhibit some surface, interfacial, and emulsification activities. Because of these properties, it is preferred over other natural hydrocolloids as an excellent ingredient for various food applications. As a hydrocolloid, fenugreek gum provides texture, appeal, gelling, thickening, emulsifying, stabilizing, and encapsulating properties. In the present review, we summarized the extraction, purification, chemical, functional properties, molecular structure, thermal and rheological behavior of gum isolated from fenugreek seeds, and then vital application of this traditional source of hydrocolloids in food, pharmaceutical, and other industries.

Physical Properties of Potyvirus on Chilli (Capsicum annuum) of Doon Valley in Uttarakhand

A virus is transmitted mechanically in the plant host. Potyviridae is a family of Potyvirus,consisting of a positive-sense RNA genome and filamentous particles. It has a single typeof protein coat. Mostly, this virus is identified in Leguminosae and Solanaceae family. Theplant virus is transmitted by mechanical inoculation and aphids. Chilli, cucurbits, potatoes,peas, tomatoes, and other plants were infected with the bean yellow mosaic virus, pea mosaicvirus, bean yellow mosaic virus, sugarcane mosaic virus, and peanut mosaic virus. Thermalinactivation point, dilution endpoint, longevity in vitro, and longevity in vivo were used toidentify the physical properties of a potyvirus. Several isolates mild and severe were collectedin the chilli plant. The infected plant developed necrotic leaf spots and chlorotic symptoms.

Exploring the Host Range of Rose rosette Virus among Herbaceous Annual Plants.

To study the host range of Rose rosette virus (RRV), we employed crude sap inoculum extracted from RRV-infected roses and the RRV infectious clone. We inoculated plants from the families Solanaceae, Cucurbitaceae, Leguminosae, Malvaceae, Amaranthaceae, and Brassicaceae. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect RRV in the inoculated plants throughout their growth stages. Interestingly, RRV was detected in the newly developed leaves of tomato, pepper, tobacco, cucumber, squash, zucchini, pumpkin, pea, peanut, soybean, spinach, okra, and Chenopodium spp. The speed of upward advancement of RRV within infected plants was variable between plants as it took two to three weeks for some plant species and up to five weeks in other plant species to emerge in the newest leaves. No severe symptoms were detected on most of the inoculated plants. Chenopodium spp., spinach, cucumber and Nicotiana rustica exhibited either chlorotic or necrotic lesions with variable shapes and patterns on the systemically infected leaves. Double membrane-bound particles of 80-120 nm in diameter were detected by transmission electron microscopy in the infected tissues of cucumber, pepper, and N. benthamiana plants. This finding infers the validity of mechanical inoculation for RRV on a wide range of plants that would serve as potential natural reservoirs.

Photosynthesis of hybrid silver-based nanoparticles mediated lectins and evaluation of their hemagglutinating properties.

Lectins are carbohydrate-binding proteins belonging to the Leguminosae family. In this family stand out proteins extracted from species belonging to Diocleinae subtribe, which includes, for example, the seed lectin from Dioclea violacea (DVL) and the jack bean lectin Concanavalin A (ConA). Here, we report the photosynthesis of silver/silver chloride nanoparticles (NPs) assisted by ConA and DVL. The syntheses were simple processes using a green-chemistry approach. Under electron microscopy, NPs heterogeneous in size, nearly spherical and covered by a thin lectin corona, were observed. Both NPs assisted by lectins were capable to cause strong rabbit erythrocytes agglutination with the same titers of hemagglutinating activities. These results indicate that both lectins maintained their biological activities even after association with the NPs and therefore are able to interact with biological membrane carbohydrates. However, for rabbit erythrocytes treated with proteolytic enzymes were observed different titers of hemagglutinating activities, suggesting differences in the spatial arrangement of the lectins on the surface of the NPs. This study provides evidences that these hybrid lectin-coated silver/silver chloride NPs can be used for selective recognition and interaction with membrane carbohydrates and others biotechnological applications.

Silencing of a mannitol transport gene in Phelipanche aegyptiaca by the tobacco rattle virus system reduces the parasite germination on the host root

ABSTRACT Root parasitic weed Phelipanche aegyptiaca is an obligate plant parasite that causes severe damage to host crops. Agriculture crops mainly belong to the Brassicaceae, Leguminosae, Cruciferae, and Solanaceae plant families affected by this parasitic weed, leading to the devastating loss of crop yield and economic growth. This root-specific parasitic plant is not able to complete its life cycle without a suitable host and is dependent on the host plant for nutrient uptake and germination. Therefore, selected parasitic genes of P. aegyptiaca which were known to be upregulated upon interaction with the host were chosen. These genes are essential for parasitism, and reduced activity of these genes could affect host-parasitic interaction and provide resistance to the host against these parasitic weeds. To check and examine the role of these parasitic genes which can affect the development of host resistance, we silenced selected genes in the P. aegyptiaca using the tobacco rattle virus (TRV) based virus-induced gene silencing (VIGS) method. Our results demonstrated that the total number of P. aegyptiaca parasite tubercles attached to the root of the host plant Nicotiana benthamiana was substantially decreased in all the silenced plants. However, silencing of the P. aegyptiaca MNT1 gene which encodes the mannitol transporter showed a significantly reduced number of germinated shoots and tubercles. Thus, our study indicates that the mannitol transport gene of P. aegyptiaca plays a crucial role in parasitic germination, and silencing of the PaMNT1 gene abolishes the germination of parasites on the host roots.

Detection of Toxicogenic Molds in Some Legumes Sold in Local Markets of Ho, Ghana

Background: Legumes are plants that contain edible seeds and belong to the family Leguminosae with varying nutritional benefits to humans and animals. This study aimed to detect and identify toxicogenic molds on some legumes purchased from two local markets in Ho Municipality, Ghana.
 Methods: A total of 36 samples, including cowpea (n=9), soybean (n=9), brown bean (n=9), and Bambara bean (n=9) were randomly obtained from 2 local markets in the Volta region of Ghana. Culturing of the legume seeds were done on mycological media using serial dilution technique. Fungal species occurrence was also determined. Statistical Package for Social Sciences (SPSS) version 26 was used to analyze the data.
 Results: Fungal counts on cowpea, soybean, brown beans, and Bambara beans ranged between 1.91 and 2.84 log Colony Forming Units (CFU)/g on both media. There were no statistically significant differences (p>0.05) in the samples from the different vendors. The Moisture Content (MC) ranged between 6.74 and 12.15%, pH ranged between 6.27±0.03-6.53±0.02. A total of 13 fungal species belonging to 7 genera were isolated on SDA and OGYEA media; Aspergillus species (A. niger, A. terreus, A. flavus, A. fumigatus, A. ochraceus, A. parasiticus), Fusarium species (F. oxysporum), Trichoderma harzianum, Rhizopus species (R. stolonifer), Penicillium species (P. digitatum, P. verucosum), Rhodotorula mucilaginosa, and Mucor racemosus were recorded on the legumes.
 Conclusion: The presence of some mycotoxigenic fungi in legumes examined in this study showed the potential health hazards in the local people of Ho, Ghana.

Molecular Breeding and Drought Tolerance in Chickpea.

Cicer arietinum L. is the third greatest widely planted imperative pulse crop worldwide, and it belongs to the Leguminosae family. Drought is the utmost common abiotic factor on plants, distressing their water status and limiting their growth and development. Chickpea genotypes have the natural ability to fight drought stress using certain strategies viz., escape, avoidance and tolerance. Assorted breeding methods, including hybridization, mutation, and marker-aided breeding, genome sequencing along with omics approaches, could be used to improve the chickpea germplasm lines(s) against drought stress. Root features, for instance depth and root biomass, have been recognized as the greatest beneficial morphological factors for managing terminal drought tolerance in the chickpea. Marker-aided selection, for example, is a genomics-assisted breeding (GAB) strategy that can considerably increase crop breeding accuracy and competence. These breeding technologies, notably marker-assisted breeding, omics, and plant physiology knowledge, underlined the importance of chickpea breeding and can be used in future crop improvement programmes to generate drought-tolerant cultivars(s).

Millettia isoflavonoids: a comprehensive review of structural diversity, extraction, isolation, and pharmacological properties.

The online version contains supplementary material available at 10.1007/s11101-022-09845-w.

Microencapsulation of Cassia fistula Flower Extract with Chitosan and its Antibacterial Studies.

The plant used in the present study is Cassia fistula, which belongs to the family Leguminosae and has been used in traditional medicinal systems due to the presence of a copious amount of Phytochemicals with various properties. This study is focused on the extraction of phytochemicals from the Cassia fistula flower and its subsequent encapsulation into chitosan matrix for applications in drug delivery. Chitosan is approved by FDA for its use in Pharmaceutical industries. As described by several analytical techniques such as FT-IR, NMR, Thermal analysis, and SEM, the microsphere thus prepared by the current study is predicted to release the desired extract with medicinal properties in a controlled manner, allowing for more convenient and desired levels of drug administration. The swelling study and release study of the prepared microsphere have been carried out in physiological pH 2 and 7.4. NMR study has shown that sitosterol and friedelin have been encapsulated successfully into the chitosan matrix. The microspheres have shown up to 80% swelling in pH 2 upto 8 days, and 60% of the in- -vitro controlled drug release has also been found in pH 2 upto 2 days. The thermal studies using TGA and DSC supported the thermal stabilities of CS beads, CFFE and CFFE-CS beads, Also, it showed the dispersion of the CFFE in the cavities of the Chitosan matrix. The Biomedical application of the synthesized CFFE-CS beads has also been reported on the basis of their antibacterial studies.

Salt-tolerant PGPR strain Priestia endophytica SK1 promotes fenugreek growth under salt stress by inducing nitrogen assimilation and secondary metabolites.

Soil salinity is a huge obstacle in crop production worldwide. Saline soil can reduce active chemical contents in medicinal plants of the Leguminosae family through crippled normal nodule function. Intensive efforts are underway to improve yield and medicinal value of leguminous herbs under salt stress condition by using benign microbes. Here, an attempt was made to explore the salt-tolerant bacteria associated with rhizosphere of fenugreek plant (Trigonella foenum-graecum L.) and to evaluate their impact on host plant growth and metabolite of pharmaceutical importance. A salt-tolerant plant growth promoting rhizobacterial (PGPR) strain Priestia endophytica SK1 isolated from fenugreek rhizospheric soil, which increased biomass and metabolite content in plants grown under saline stress. SK1 bacterial application induced nodule formation and enhanced nitrogen and phosphorus content under salt (100 mM NaCl) stress as compared to control plants. H2 O2 production and lipid peroxidation as a measure of stress were observed high in control plants, while a reduction in these parameters was observed in plants inoculated with SK1. In addition, a significant effect was found on the phenolic compounds and trigonelline content in fenugreek plant inoculated with SK1 bacterium. An increased trigonelline content of about 54% over uninoculated control was recorded under salt stress. The results of this study revealed that the application of salt-tolerant PGPR strain P. endophytica SK1 induced nitrogen fixation machinery that leads to alleviate salt stress and improved the biosynthesis of trigonelline content in fenugreek. This study extends our understanding on the significance of rhizosphere microbiome and their beneficial role in plant health under environmental stress to promote agro-eco-farming practices.

Pharmacognostical standardization of Cassia auriculata, Centella asiatica and Zingiber officinale

Cassia auriculata, Centella asiatica and Zingiber officinale are belongs to the family Leguminosae, Apiaceae and Zingiberaceae respectively. These herbs possess abundant remedial benefits and are used traditionally to treat numerous illnesses. The standardization of these crude drugs is essential to gain information on their identity and quality. WHO recommended parameters were used to standardize the plant materials. Dried raw powder of Cassia auriculata leaf, Centella asiatica leaf and Zingiber officinale rhizome were subjected to macroscopic and microscopic evaluation followed by physicochemical evaluations such as loss on drying, ash values and extractive values. Further, the plant materials were extracted with hydroalcoholic solvent (Alcohol: Water ratio is 70:30) by cold maceration process and subjected to qualitative phytochemical investigation. The identity and purity of the plant material had been proven by its organoleptic characteristics. Detailed structural characteristics were obtained from powder microscopy. Physico-chemical parameters displayed the quality of raw powder. Qualitative phytochemical analysis showed the presence of various phytoconstituents. The pharmacognostical standardization has provided with a referential information on the identity, quality and purity of the crude drugs.

서로 다른 인공광원 하에서 기내 발아 및 배양된 새싹 황기의 생육 특성및 생리활성 검정

Background: Astragalus membranaceus Bunge is a plant belonging to the Leguminosae family that is used in oriental medicine as a diuretic or a tonic and has blood pressure-lowering properties. Several other propertieds, including reduction of blood sugar, antitumor, and antiviral properties, have also been reported.BRMethods and Results: A. membranaceus treated exposed to blue light showed the maximum value for plant height, root length, and leaf number. Antioxidant activity was also observed under white light. The maximum values of total phenol and total flavonoid content were observed under white light and blue light, respectively. To assess the whitening and anti-wrinkle activities, the tyrosinase inhibiting activity was determined, which showed the highest value at the maximum concentration of 5,000 ㎍/㎖ in blue light-treated A. membranaceus.BRConclusions: The use of LED light will benefit the commercial production of A. membranaceus for cosmetic and food purposes.

Screening of metabolic markers present in Oxytropis by UHPLC-Q-TOF/MS and preliminary pharmacophylogenetic investigation.

Plants belonging to the Oxytropis genus, family Leguminosae, are found throughout the world, with about 80 species mainly distributed in northwest and northeast China. The plants have medicinal properties and many plants have been used as folk medicine for the treatment of colds, inflammation of carbuncle swelling, pain, and different types of bleeding. In recent years, due to the reduced availability of wild resources and increased clinical demand, additional Oxytropis species have been used in Mongolian medicine. This study explored the medicinal potential of four Oxytropis species, investigating their phylogeny, chemical components, and pharmacological activities. Oxytropis myriophylla (Pall) DC., Oxytropis hirta Bunge, and Oxytropis bicolor Bge. were found to be closely related at the taxonomic level. While previous investigations on the bioactive constituents of Oxytropis have been limited and have concentrated largely on flavonoids and saponins, the present study established a novel UHPLC-Q-TOF/MS based on metabolite profiling to comprehensively analyze the chemical composition of the four Oxytropis species and to identify marker compounds. A total of 75 compounds were identified from the four species, with 23 identified as characteristic marker components. Twenty-six marker compounds were identified in O. myriophylla from different geographical regions. Analysis of pharmacological activity showed that extracts of O. myriophylla and O. hirta had stronger anti-inflammatory activity than the extracts from the other species. The relationships between the chemical components, traditional curative uses, and pharmacological activities were analyzed to provide a preliminary documentation of the pharmacophylogenetic characteristics of the Oxytropis family as a whole. Several marker compounds, including licoricesaponin G2, licoricesaponin J2, and glycyrrhizic acid found in O. hirta were found to have effective anti-inflammatory activity, consistent with the traditional application of reducing swelling and healing wounds. This preliminary investigation into the pharmacophylogeny of the genus Oxytropis will contribute to the conservation and exploitation of the medicinal resources of this genus.

Effect of Soil Amendments with Botanical Leaves on Root-Knot Nematode (Meloidogyne incognita) of Cowpea (Vigna unguiculata L.)

Cowpea (Vigna unguiculata L. Walp) is a earliest crop which is a member of the Phaseoleae tribe of the Leguminosae Family widely known as Lobia, which is a better alternative source for protein, minerals etc., as compared to other legumes. Root-knot nematodes (Meloidogyne incognita) cause a major loss in cowpea production by hindering nutrient and water uptake. Leaf waste of marigold, rose, calotropis, euphorbia, neem and castor as botanical amendments were experimented under field conditions during kharif 2021 for their effectiveness against the root knot nematodes, rhizobium nodule and growth parameters. Field studies were conducted in randomized blocks with six treatments and three replications. Among the composts, the treatment Neem leaf compost remarkably increased the Plant height at 90 DAS (293 cm), root length at 90 DAS (29.16cm), rhizobium nodules (5), and significantly decreased the root weight at 90 DAS (3.5 gm) and root knots in the root system of cowpea at 90 DAS (10.67). The weight of the roots decreased because of a decrease in the number of root knot in cowpea compared to other composts and control T0.

Phylogenetic analyses of Phaseolinae (Leguminosae) reveal a new genus endemic to limestone outcrops in Seasonally Dry Forests of Bahia, Brazil

AbstractPhaseolinae is one of the most diverse groups within the family Leguminosae, characterized by a style bearing a pollen brush and seeds with an epihilum. This subtribe has a complex taxonomic history and has undergone several changes over time. In the early 2000s, our research team found a phaseoloid liana growing in a limestone outcrop in western Bahia, Brazil. Its identity remained a mystery until recently, when we revisited the locality to study it in more detail and gathered material to generate nuclear ITS and plastid trnK/matK sequences. These sequences were included in a broadly sampled Phaseolinae matrix used to perform phylogenetic analyses and an ancestral character states reconstruction. Our results demonstrate that the enigmatic taxon represents a new lineage within subtribe Phaseolinae, sister to the genus Sigmoidotropis. This new lineage, along with Sigmoidotropis and Ancistrotropis, forms a clade here called the Sigmoid‐keel clade, since all representatives present flowers with sigmoid keel petals. The new taxon is here described as Delgadoa bambuicola gen. & sp. nov., a new genus and a new species of woody lianas known from only two localities in the state of Bahia. We also present an identification key to the genera of the Sigmoid‐keel clade, descriptions, illustrations, a distribution map, taxonomic comments, and a conservation status assessment of the new genus.

PHYTOCHEMICAL ANALYSIS AND TOTAL TANNIN CONTENT (TTC) OF DELONIX REGIA (BOJER EX. HOOK) RAF. BARK BY USING DIFFERENT SOLVENTS COLLECTED FROM SAURASHTRA REGION

Delonix regia (Bojer ex. hook) Raf. is belongs to Leguminosae (Fabaceae) family and Sub family Caesalpiniodeae. Usually it’s called gulmahor in Gujarati. It is used as an ornamental plant but it has some bioactive constituents which are useful in medicinal level. Delonix regia (Bojer ex. hook) Raf. is a beautiful, semi-deciduous and roadside avenue tree. It is cultivated in rural and urban area in India. It has medicinal properties such as antimicrobial and antifungal activities or as antibiotics. In this present study, qualitative and quantitative estimation of Delonix regia (Bojer ex. hook) Raf. bark from Saurashtra region. The preliminary phytochemical analysis and quantification for total tannin content was performed by using two different solvents (Acetone and Ethanol). The phytochemical analysis revealed that the presence of various phytochemicals such as phenols, tannins, flavonoids, proteins etc. Based on experiment, various bioactive constituents were present so it’s important for pharmaceutical level.

Genetic Variability, Correlation Co-efficient and Path Analysis in Soybean (Glycine max L. Merr.) Genotypes

Soybean (Glycine max L. Merr.) is an important oil seed and grain legume crop in the world and a perspective high potential crop in Bangladesh. It belongs to the family Leguminosae, sub-family papilionaceae and genus Glycine. An experiment was conducted with 14 genotypes of soybean (Glycine max L. Merr.) in a randomized complete block design (RCBD) to study the variability, correlation coefficient, and path analysis. Analysis of variance for each trait showed significant differences among the genotypes. The genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were close to each other for all the characters except the leaf area index, indicating the minor environmental influence on the expression of these characters. High heritability associated with high genetic advance in percent of mean was observed for leaf area index, number of seeds per plant, 100 seed weight, and yield, which pointed out that selection for these characters, would be effective. The significant positive correlation with seed yield was found in pod length and 100 seed weight which revealed that selection based on these traits would improve yield ultimately. Path coefficient analysis evidenced that 50% flowering, pod length, days to 1st pod maturity, number of seed per plant and 100 seed weight had a positive direct effect on yield per plant. Therefore, importance must be given to these characters in further breeding programs to improve soybean yield.

EFFECT OF PRE-SOWING TREATMENT ON SEED GERMINATION OF LOHAKATH (XYLIA KERRII CRAIB & HUTCH.)

Lohakath (Xylia kerrii Craib & Hutch.), a valuable timber species in Bangladesh, belongs to the family Leguminosae. Morphology of fruits, seeds and the effect pre-sowing treatments of seeds were studied. The average length, breath and thickness of fruits were found 13.3, 5.3 and 0.8 cm respectively and those of seeds were 1.4, 0.3 and 0.3 cm respectively. Number of seeds per kilogram was 5000-5400. The germination test was done by sowing the seeds in polybags (4 cm x 6 cm) filled with a mixture of topsoil, coconut husk compost, coarse sand and fine sand in the ratio of 3:4:1:1. The highest germination percentage (98%) was found in scarification with sand paper. The lowest germination percentage (53%) was found in control. About 87% was found in hot water (80 °C) immersion (10 minutes), 84% was found in 80% concentrated H2SO4, 63% was found in immersion in cold water (4 °C) for 12 hours. As the use of sulphuric acid and scarification techniques are somewhat risky and trouble some, therefore, pre-sowing treatment of Lohakath seed for germination in rural Bangladesh could be hot water immersion method.