Fenugreek Seed Extract Research Articles

Fenugreek seed extract attenuates cisplatin-induced testicular damage in Wistar rats.

Cisplatin (CIS) provides oxidative stress and inflammations in testicular tissues. Fenugreek seed extract (FSE) is a widely used herbal medicine with potent antioxidant and anti-inflammation properties. The purpose of this study was to investigate the protective effects and the possible mechanisms of FSE against CIS-induced testicular damage in rats. Adult male Wistar rats were given vehicle, single dose of CIS alone (10 mg kg(-1)), single dose of FSE alone or single dose of CIS followed by FSE (50, 100 or 200 mg kg(-1)) every day for 5 days. On day 6, oxidative stress and apoptotic testicular toxicity were evaluated. FSE attenuated both germ cell degenerations and apoptosis in seminiferous tubules in CIS-treated rats. Furthermore, FSE counteracted CIS-induced oxidative stress in rats as assessed by the restoration of superoxide dismutase and catalase activities and reduction in the myeloperoxidase activity and malondialdehyde levels in testes. CIS increased expressions of inducible nitric oxide synthase and nuclear factor-kappa B in testicular tissues. Importantly, treatment with FSE at all doses effectively alleviated all of these inflammatory parameters in testes. Based on these results, we concluded that FSE reduces CIS-induced reproductive toxicity in rats by the suppression of testicular oxidative stress, apoptosis and inflammations.

Efficacy of a Novel Fenugreek Seed Extract (Furocyst) in Polycystic Ovary Syndrome in Female Subjects

Polycystic ovary syndrome (PCOS) is the most prevalent hormonal disorder among women of reproductive age causing irregular menstrual cycles, excessive body or facial hair, miscarriage and infertility. Infertility is one of most common PCOS symptoms. Because the symptoms of PCOS are seemingly unrelated to one another, the condition is often overlooked and undiagnosed. We conducted an open label, one‐arm, non‐randomized, post‐marketing surveillance study in 50 premenopausal women (18‐45 years, BMI<42) diagnosed with PCOS using a novel fenugreek seed extract (Furocyst, 2 capsules of 500 mg each/day) over a period of 90 consecutive days to determine its efficacy on the reduction of ovarian volume and the number of ovarian cysts. Ethical committee approval was obtained for this study. Furocyst caused significant reduction in ovary volume. Approximately 46% of study population showed reduction in cyst size, while 36% of subjects showed complete dissolution of cyst. It is important to mention that 71% of subjects reported regular menstrual cycle on completion of the treatment and 12% of subjects got pregnant. Overall, 94% of patients were benefitted from this study. Significant increases in luteinizing hormone (LH) and follicular stimulating hormone (FSH) levels were observed compared to the baseline values. Extensive blood chemistry, hematological and biochemical assays demonstrated the broad spectrum safety. Furocyst caused significant decrease in both ovarian volume and the number of ovarian cysts. In summary, Furocyst was efficacious in ameliorating PCOS.

Administration of Fenugreek Seed Extract Produces Better Effects in Glibenclamide-Induced Inhibition in Hepatic Lipid Peroxidation: An in vitro Study.

To evaluate the comparative effects of fenugreek (Trigonella foenum graecum) seed extract (FSE) alone and in combination with an antidiabetic conventional medicine, glibenclamide (GLB), on the inhibition of in vitro lipid peroxidation (LPO) in liver, the major target organ of a drug. LPO was induced by ferrous sulphate (FeSo4), hydrogen peroxide (H2O2) and carbon tetrachloride (CCl4) and the effects of test seed extract and/or GLB were evaluated. While FeSo4, H2O2 and CCl4 markedly enhanced the hepatic LPO, simultaneous administration of FSE reduced it in a concentration dependent manner. However, when both FSE and GLB were added to the incubation mixture, chemically induced hepatic LPO was further inhibited. The test extract also exhibited high antioxidative activity in 1,1-diphenyl-2-picrylhydrazyl radical and in 2,2'-azinobis, 3-ethylbenzothiazoline-6-sulphonic acid radical scavenging assays. FSE therapy in moderate concentration along with a hypoglycemic drug may prove to be advantageous in ameliorating diabetes mellitus and other diseases that are LPO mediated.

Effects of Fenugreek Seed Extract and Swimming Endurance Training on Plasma Glucose and Cardiac Antioxidant Enzymes Activity in Streptozotocin-induced Diabetic Rats

ObjectiveDiabetes mellitus is a group of metabolic diseases characterized by chronic hyperglycemia condition resulting from defective insulin secretion or resistance insulin action, or both. The purpose of this study was to evaluate the effect of 6 weeks swimming training and Trigonella foenum-graecum seed (fenugreek) extract, alone and in combination, on plasma glucose and cardiac antioxidant enzyme activity of streptozotocin-induced diabetic rats. MethodsFifty male Wistar rats were divided into five groups: diabetic control (DC, n = 8); healthy control (HC, n = 11); swimming training (S, n = 11); swimming training + fenugreek seed extract (1.74 g/kg body weight; SF1, n = 11); and swimming training + fenugreek seed extract (0.87 g/kg body weight; SF2, n = 9). Streptozotocin was used for the induction of diabetes. Results were analyzed using one-way analysis of variance followed by Tukey test. ResultsIn comparison with the DC group, all groups exhibited a significant decrease in body weight (p < 0.05), except for the HC group. SF1 and HC groups showed significant decreases in plasma glucose levels compared with the DC group (p < 0.05). S, SF1, SF2, and HC groups showed significant elevations in cardiac antioxidant enzymes activity in comparison with the DC group. ConclusionThe results indicated that the combination of endurance swimming training and fenugreek seed extract can significantly reduce the plasma glucose levels and increase cardiac antioxidant enzymes activity in diabetic rats. Our findings suggest that this combination could be useful for the treatment of hyperglycemia and cardiac oxidative stress induced by diabetes mellitus.

Effects of fenugreek, Nigella, and termis seeds in nonalcoholic fatty liver in obese diabetic albino rats

Background and study aimNonalcoholic fatty liver disease (NAFLD) occurs in approximately 80% of cases of type 2 diabetes mellitus (T2DM). This study investigated the effects of some plants used in Saudi Arabia as antidiabetic agents on T2DM and associated fatty liver. Materials and methodsA total of 150 adult male albino rats were divided into six experimental groups, each consisting of 25 rats. Twenty-five rats were considered as the control group. Experimental diabetes was induced in the remaining rats by administering a subcutaneous injection of 120mg/kg of freshly prepared alloxan solution; these rats were classified into five groups: one group did not receive any treatment; the second group was treated with an aqueous extract of a mixture containing fenugreek, Nigella, and termis seeds; the third group was treated with an aqueous extract of Nigella sativa seeds; the fourth group was treated with an aqueous extract of fenugreek seeds; and finally the fifth group was treated with an aqueous extract of termis seeds at a dose of 100mg/kg body weight. After 4weeks of treatment, biochemical parameters were calculated, including blood sugar and serum insulin levels. Pancreatic and liver samples were obtained and processed for microscopic evaluation. ResultsThe usage of each plant alone or a mixture of the plants corrected the glucose and insulin levels. Microscopically, a definite improvement in the number and diameter of β-cells in the diabetic group was observed. Furthermore, considerable improvement in fatty changes occurring in the liver of experimental animals was observed. The activity of the mixture was the most effective. ConclusionsThe aqueous extract of the seed mixture of the used plants appeared to be a useful agent in improving fatty changes in the liver texture associated with T2DM by reducing hyperglycaemia through an increase in insulin levels, regeneration of β-cells of the pancreas, and an amelioration of associated dyslipidemia.

English

English

Prophylactic efficacy and possible mechanisms of oligosaccharides based standardized fenugreek seed extract on high-fat diet-induced insulin resistance in C57BL/6 mice

Preclinical safety assessment of glycosides based standardized fenugreek seeds extract: Acute, subchronic toxicity and mutagenicity studiesPallavi Deshpande, Vishwaraman Mohan, Prasad Thakurdesai

Fenugreek seed extract and its phytocompounds- trigonelline and diosgenin arbitrate their hepatoprotective effects through attenuation of endoplasmic reticulum stress and oxidative stress in type 2 diabetic rats

Fenugreek seed and some of its phytoconstituents are known to possess hypoglycemic and hypolipidemic properties but their mode of action in insulin target tissues remains to be elucidated. As hepatic insulin resistance contributes to the development of type 2 diabetes mellitus (T2DM), the protective effects of fenugreek seed extract (FSE) and two of its phytoconstituents-trigonelline and diosgenin were evaluated in T2DM rats with special focus on changes in endoplasmic reticulum (ER) stress and oxidative stress markers in liver. Trigonelline and diosgenin content in FSE were quantified by HPTLC. T2DM was induced in male Sprague–Dawley rats by feeding high-fat diet and administration of low-dose streptozotocin. T2DM rats exhibited hyperglycemia and significantly reduced serum C-peptide levels. Liver damage in T2DM rats was marked by significantly elevated levels of liver marker enzymes in serum, liver triglycerides (TGs), and reduced liver glycogen and was further confirmed by histological analysis. T2DM rats also displayed twofold–threefold increase in the levels of ER chaperones Bip, protein disulfide isomerase (PDI) as well as ER stress associated proapoptotic markers CHOP, Caspase12 and Caspase3 in liver along with elevated lipid peroxidation (LPO) and reduced antioxidant levels. Promisingly, FSE, trigonelline, and diosgenin were found to exhibit protective effects individually and brought about significant reduction in serum enzymes, liver TGs, LPO, expression of liver ER stress marker proteins, and significant increase in liver glycogen content and antioxidants. Histological analysis also supported their protective effects suggesting that FSE and its phytoconstituents alleviate T2DM associated liver damage by normalizing ER stress and oxidative stress.

Protective effect of fenugreek seeds (Trigonella foenum graecum) extract against experimental gastric ulcer in rats

Trigonella foenum graecum, (Fenugreek) is used in traditional medicine for its anti-inflammatory, antiseptic, and antidiabetic properties. The present study was designed to investigate the protective effects of fenugreek seed extract on experimentally induced gastric mucosal ulcer in rats. In this study, 24 male rats were randomly divided into 4 groups. Control group underwent gastric ulcer (by ligating the pylorus). Treatment group was given fenugreek seeds extract via oral gavage for 1 week before inducing gastric ulcer. Groups sham I and II were given normal saline and extract orally, respectively, without inducing gastric ulcer. After the experiment, the glandular stomach was removed and the tissues were processed for macroscopic and histopathologic examination. The results of present study showed that administration of fenugreek seeds extract significantly decreased erosion and ulcer in the treatment group compared to the control group. Significant increases in the number of inflammatory cells were observed in the control group while no noticeable lesions were visible in the gastric mucosa of treatment group. Normal gastric mucosa was observed in the groups sham I and II. According to the results of the current study, fenugreek seeds extract oral usage can protect the gastric mucosa from injury.

Identification and quantification of flavonoid glycosides from fenugreek (Trigonella foenum-graecum) germinated seeds by LC–DAD–ESI/MS analysis

The phenolic composition of fenugreek (Trigonella foenum-graecum) germinated seeds originating from Morocco has been investigated. After germination, extraction was performed from defatted germinated seeds by a hydromethanolic solution using an accelerated solvent extractor. HPLC coupled to negative ion electrospray ionization mass spectrometry and diode array detection was employed to identify the polyphenol in the obtained fenugreek germinated seeds extract. A total of 28 identified phenolic compounds were grouped into flavone di C-glycosides, flavonol O-diglycosides, flavone tri- and tetra O-, C-glycosides and acylated flavone O-, C-glycosides. Most of the identified flavones were apigenin and luteolin adducts, while only two kaempferol glycosides were detected as flavonols. Quantitative analysis of the identified compounds showed that the phenolic composition of the studied germinated fenugreek seeds was predominated by acylated and non-acylated flavone derivatives with apigenin as main aglycone. Germinated fenugreek seeds could be considered as a rich source of bioactive phenolic compounds.

Effects of fenugreek seeds on adipogenesis and lipolysis in normal and diabetic rats.

Several studies support hypolipidemic effect of fenugreek in normal and diabetic subjects. However, very little is known about the possible direct action of fenugreek on adipose tissue. The present study was designed to investigate the effects of fenugreek seeds on adipogenesis and lipolysis. Preadipocytes were isolated from adipose tissue of normal rats and differentiated to adipocyte in the presence of ethanolic extract of fenugreek seeds. The effect of this extract on lipolysis was also evaluated in fat tissue isolated from diabetic rats. Fenugreek led to a significant reduction in lipid droplet accumulation as evaluated with Oil Red O staining. Incubation of preadipocytes with the extract for 24 h resulted in significant decrease in cell viability. The extract, even at high concentrations (up to 1000 μg mL(-1)), had virtually no significant effect on lipolysis. The present data demonstrated that fenugreek seed inhibits formation of new differentiated adipocytes from precursor cells through an anti-proliferative effect on preadipocytes.

A Mechanism-based Pharmacological Evaluation of Efficacy of Trigonella foenum graecum (Fenugreek) Seeds in Regulation of Dyslipidemia and Oxidative Stress in Hyperlipidemic Rats

: Alcoholic extract of Trigonella foenum graecum seeds [fenugreek seed extract (FSE)] was studied in triton-induced and high-fat diet-induced hyperlipidemia to evaluate antidyslipidemic effect. Plasma cholesterol (26.19%) and triglycerides (36.6%) were found to be lowered by FSE maximum at a dose of 200 mg/kg body weight in triton-treated hyperlipidemic rats. Chronic feeding of FSE (200 mg/kg body weight) caused lowering in plasma and hepatic lipid levels by activating lecithin-cholesterol acyltransferase (47%), postheparin lipolytic activity (35%), triglyceride lipase (34%), lipoprotein lipase (20.8%), and increased excretion of fecal bile acids (36%-45%). The FSE shows potent antioxidant activity in both in vitro and in vivo systems. It inhibited generation of superoxide anion and hydroxyl free radicals in both enzymatic and nonenzymatic systems significantly at 200 µM concentration. Furthermore, FSE normalizes the activities of antioxidant enzymes, that is, superoxide dismutase and catalase, and reduces plasma lipid peroxidation (33.9%), hepatic 4-hydroxynonenal (27%), and isoprostanes (28%). Data of the present study demonstrated that the T. foenum graecum seed extract has both antidyslipidemic and antioxidant properties.

Fenugreek seed extract treats peripheral neuropathy in pyridoxine induced neuropathic mice.

Trigonella foenum graecum commonly known as Fenugreek exerts normoglycemic and insulinotropic effects in humans by compounds from its seed and leaf extracts. Some studies reported that treating pregnant mice with fenugreek seed could cause toxic effects on the nervous system of its pubs during developmental growth, while in some other studies neuroprotective properties were considered for it. Safety of anti-diabetic drugs for nervous system is very important because peripheral neuropathy is a common complication of diabetes and hazardous drugs could worsen it. In this study, the effect of treatment with fenugreek seed extract on the function of sciatic nerves of neuropathic mice was evaluated. Neuropathy was induced in male mice by pyridoxine intoxication. After that, animals were treated with 0.2, 2 and 20 mg/kg of hydro-alcoholic extract of fenugreek seeds for 10 days, tail flick, electrophysiological and histological assays were performed to evaluate the effect of fenugreek seed extract on function of the peripheral nerves. Our data showed that fenugreek has anti neuropathic effect and restores the function of nerve fibers. Results of electrophysiological recordings stated that the highest rate of healing was occurred in 20 mg/kg fenugreek extract treated animals. In conclusion, findings of the present study demonstrate that treatment with fenugreek seed extract can potentially facilitate healing from pyridoxine induced peripheral neuropathy in mice.

Evaluation of Fenugreek (Trigonella foenum-graceum L.), Effects Seeds Extract on Insulin Resistance in Women with Polycystic Ovarian Syndrome.

PCOS (Polycystic Ovarian Syndrome) is associated with insulin resistance, obesity and disorders of lipid metabolism as well as infertility. Fenugreek seeds extract is successfully used in lowering blood glucose. Metformin has also the same effect but in a different way. The aim of this study was the assessment of fenugreek effects on insulin resistance in women with PCOS. This was a prospective randomized, double-blind, placebo-controlled trial. The study was conducted at the Montaserieh Hospital in Mashhad University of Medical Sciences, Mashhad, Khorasan Razavi Province, Iran. The patient population included 58 oligo-anovulatory PCOS women with typical ovaries. Women were randomly allocated to receive hydroalcoholic extract of fenugreek seeds in capsules with metformin (n = 30) or placebo capsules with metformin (n = 28) and were assessed before and every 4 weeks within a treatment period of 8 weeks. Menstrual disturbance and metabolic parameters (markers of insulin resistance and hormonal parameters) were measured. Insulin resistance based on HOMA-IR (homeostasis model assessment for insulin resistance) model was not significantly different between two groups. Ultrasound scans were performed before and at the end of 8 weeks treatment with significant decrease in PAO (polycystic appearing ovaries) in group 1 (p = 0/01). Adjuvant therapy to the fenugreek seeds extract (with metformin) in PCOS women improved the sonographic results and menstrual cyclicity.

Fenugreek extract as an inducer of cellular death via autophagy in human T lymphoma Jurkat cells

BackgroundDrugs used both in classical chemotherapy and the more recent targeted therapy do not have cancer cell specificity and, hence, cause severe systemic side effects. Tumors also develop resistance to such drugs due to heterogeneity of cell types and clonal selection. Several traditional dietary ingredients from plants, on the other hand, have been shown to act on multiple targets/pathways, and may overcome drug resistance. The dietary agents are safe and readily available. However, application of plant components for cancer treatment/prevention requires better understanding of anticancer functions and elucidation of their mechanisms of action. The current study focuses on the anticancer properties of fenugreek, a herb with proven anti-diabetic, antitumor and immune-stimulating functions.MethodJurkat cells were incubated with 30 to 1500 μg/mL concentrations of 50% ethanolic extract of dry fenugreek seeds and were followed for changes in viability (trypan blue assay), morphology (microscopic examination) and autophagic marker LC3 transcript level (RT-PCR).ResultsIncubation of Jurkat cells with fenugreek extract at concentrations ranging from 30 to 1500 μg/mL for up to 3 days resulted in cell death in a dose- and time-dependent manner. Jurkat cell death was preceded by the appearance of multiple large vacuoles, which coincided with transcriptional up-regulation of LC3. GC-MS analysis of fenugreek extract indicated the presence of several compounds with anticancer properties, including gingerol (4.82%), cedrene (2.91%), zingerone (16.5%), vanillin (1.52%) and eugenol (1.25%).ConclusionsDistinct morphological changes involving appearance of large vacuoles, membrane disintegration and increased expression of LC3 transcripts indicated that fenugreek extract induced autophagy and autophagy-associated death of Jurkat cells. In addition to the already known apoptotic activation, induction of autophagy may be an additional mechanism underlying the anticancer properties of fenugreek. This is the first report showing fenugreek as an inducer of autophagy in human cells and further work is needed to define the various intermediates of the autophagic pathway.

Preparation and evaluation of functional foods in adjuvant arthritis

Adjuvant arthritis is an animal model that closely resembles rheumatoid arthritis in humans. It is a successful working model used to study new anti-inflammatory agents. In previous studies (animal and clinical) we have shown that evening primrose oil, fish oil and the methanol extract of date fruits and fenugreek seeds have anti-inflammatory activity and that the methanol extract of dates has an antioxidant effect. Based on these studies, the aim of the present study was to prepare 7 functional foods containing such bioactive fractions separately or in combination and to evaluate them in adjuvant arthritis in rats, study the stability of bioactive ingredients and evaluate their sensory properties. The studied biochemical parameters were erythrocyte sedimentation rate, erythrocyte superoxide dismutase, glutathione peroxidase and plasma copper, zinc and interlukin 2. Nutritional parameters, including body weight gain, food intake and food efficiency ratio were monitored during the feeding of the functional foods. The bioactive ingredients assessed were total phenolic contents and fatty acids. The results showed improvement in the biochemical parameters, body weight gain and food efficiency ratio of arthritic rats fed on the functional foods with different degrees. All the prepared functional foods were sensory accepted. The active ingredients showed stability during storage. In conclusion, all the tested functional foods showed promising antiinflammatory activity and were determined to be acceptable through sensory evaluation which means that their potential beneficial use as dietary supplements in rheumatoid arthritis patients may be recommended.

English

An extract from the seeds of&nbsp;Trigonella foenum graecum&nbsp;(Fenugreek) was evaluated for its protective effect against hydrogen peroxide (H2O2) induced oxidation in normal sheep erythrocytes (red blood cells (RBCs)). RBCs, treated with increasing concentrations of 30% H2O2&nbsp;along with fenugreek seed extract (FSE) were analyzed for hemolysis and lipid peroxidation. Sheep RBCs treated with increasing concentrations of glucose, to study effect of high glucose level or hyperglycemia on normal SRBC, were found to be more susceptible to lipid peroxidation than those from normal subjects. However, on treatment with FSE, the oxidative modifications in both the groups (RBCs treated with glucose and not treated with glucose) were found to reduce significantly. The inhibition of lipid peroxidation was concentration-dependent up to 100 &mu;l of extract, which contained 0.75 mM gallic acid equivalent (GAE) of phenolic compounds. The total phenolic content in the extract was determined spectrophotometrically according to the Folin-Ciocalteau procedure and was expressed as mg or mM GAE. The results indicate that the extract of fenugreek seeds contains antioxidants and protects cellular structures from oxidative damage. These findings demonstrate the potent antioxidant properties of the fenugreek seeds. &nbsp; Key words:&nbsp;Fenugreek seed extract (FSE), type II diabetes, osmofragility test, lipid peroxidation.

Effect of trigonelline and ethanol extract of Iraqi Fenugreek seeds on oxidative stress in alloxan diabetic rabbits

This study was designed to evaluate effect of trigonelline and ethanol extract of Iraqi Fenugreek seeds on oxidative stress in alloxan diabetic rabbits.Oral dose of (10mg/12h) isolated trigonelline or (7.7ml/12h) of ethanol extract of Iraqi Fenugreek seeds was administered to the rabbits for four weeks. Plasma malondialdehyde (MDA) and glutathione (GSH), as oxidative stress markers were measured in all rabbits before treatment and once weekly for four weeks after the treatment. The results showed a significant improvement in the stress induced oxidation parameters by alloxan-induced diabetes in rabbits.The ethanol extract of Fenugreek seeds significantly decreases the plasma malondialdehyde (MDA) from 2.51±0.34μmol/l in diabetic control rabbits to 1.57±0.12μmol/l after four weeks (P<0.05). The plasma glutathione (GSH) level increases significantly from 0.11±0.02μmol/l to 0.26±0.02μmol/l after four weeks of treatment of the ethanol extract (P<0.05). The percent reduction in plasma MDA level was 37.45%, while the percent elevation in plasma GSH was 136% after four weeks of treatment. The data showed that isolated trigonelline was less effective than the ethanol extract of Fenugreek seeds in lowering plasma MDA or increasing the plasma GSH markers. The results of this study clearly indicate that both ethanol extract of Fenugreek and its major alkaloid, trigonelline are promising natural antioxidants and may be used in the treatment of many diseases, especially diabetes mellitus.

Effect of fenugreek seed extract on carbendazim‐inhibited spermatogenesis in albino rats

Carbendazim is a carbamate fungicide used in control of various fungal pathogens. Fenugreek (Trigonella foenum‐graecum Linn.) is a leguminous plant cultivated in several Asian and African countries and its seeds are used as herbal medicine. In the present work the effect of aqueous extract of fenugreek seeds on carbendazim‐induced testicular toxicity in albino rats was studied. Treating rats with carbendazim induced significant decrease in testis weights, diameters and germinal epithelial heights of the seminiferous tubules. Histological results revealed degeneration of seminiferous tubules and reduction of spermatogenic cells. Moreover, carbendazim caused elevation of testicular malondialdehyde (MDA), and reduced the activity of the antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). Animals treated with carbendazim and fenugreek showed improvement in the histomorphological and histopathological changes observed in animals treated with carbendazim. In addition fenugreek treatment leads to a significant decrease in the level of MDA and increase in the activity of SOD and CAT. it is concluded that fenugreek extract can improve the testicular toxicity of carbendazim and this effect may be attributed to its antioxidant properties.

دراسة التأثيرات السمية الخلوية للمستخلص المائي لبذور الحلبة ومعقد البلاديوم (II) الجديد على الخط الخلوي لسرطان امعاء الفئران في العراق

treat arthritis inflamed lungs, diabetes, etc. Numerous palladium complexes with promising activity against tumor cell lines have been synthesized. This study involved the evaluation of Aqueous Fenugreek seeds Extract and new palladium (II) Complex with general formula [Pdl2].2ETOH, where L=2-hydroxy phenyl piperonalidine have a promising anitcancer effect on growth cancer cells and were compared to the anti cancer drug cis-ptatinum by utilizing an in vitro system in femal Balb/c mice by using the Inhibitor Rate as a parameter for the cytotoxic effects on Intestine cancer cell line L20B. Cancer cells were treated with four concentrations of cis-platin 31.25,62.5,125, and 250 μg/ml for 48 and 72 hours .The same concentrations were used for the other extract and complex. This study showed that extract and [PdL2]. 2EtOHcomplex have a promising anti cancer cells as could be seen from these effect on Increased of Inhibition cancer cells at different concentrations and these effect were similar to the effect of cis- platin, then the Inhibitory Rate was increased to 44.60,52.32% and 37.93, 42.97% in cancer cells treated with 250 μg/ml of palladium (II) complex and extract respectively on hours exposure with not singnificant differences with compared Cis- platin.