Extract Of Achyranthes Aspera Research Articles

Preliminary physico – phytochemical and phytocognostical evaluation of the leaves parts and evaluation of herbal ointment using leaves of Achyranthes aspera L. Leaf extract

Herbal plants are huge sources of neutraceuticals, prevent the different disease or maintain healthy life. Plant produces primary metabolites for their basic survival and secondary metabolites for their ecological, taxonomical and biochemical differentiation and diversity. Achyranthes aspera L. (Family Amaranthaceae) are broadly used in traditional system of medicine throughout different part of India, South Andaman Island, Pacific Island, Baluchistan, Tropical Asia, America, and Australia. It has great medicinal importance like to stomach tonic, diuretic, laxative, anthelmintic, anti hyperlipidemic, expectorant, anti-inflammatory, anti-bacterial, anti-fungal, hypoglycemic, anti-asthmatic and anti-allergic etc. The study of diseases and their treatment are important part of our ancient time worldwide. The knowledge of medicinal plants must have been accumulated in the course of many centuries. Herbal medicine prepare different part of plant are used. Herbal drug is design as the alternative formulation for the external use in the form of ointment. For the clinical use the herbal ointment apply externally on human body. The main aims of this research are Preliminary physico–phytochemical & phytocognostical evaluation of the leaves parts and preparation of herbal ointment from the Achyranthes aspera. Achyranthes aspera L. plants used as antibacterial activity. Under this research work, it is design as herbal ointment with the help of Ethanolic extract of Achyranthes aspera and determines the anti-bacterial capacity on it. In vitro study the antibacterial activity of the Ethanolic extracts of dried leaves of Achyranthes aspera L. are determine by using the Agar cup plate method versus different bacteria such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa etc. By blending the Ethanolic extract of Achyranthes aspera L. (10 % w/w) into aqueous cream we formulated herbal ointment. The emulsifying agent and simple ointment bases are added in above herbal ointment for increase the antibacterial effectiveness during in vitro evaluation. The current study deals with the characterization of morphological features, determination of physical constant such as the total cash value, water soluble ash value were 11.25%, 5.25% respectively. Loss of weight drying was 12.9%, foaming index>100, swelling index were1.5cm, the percent yield for petroleum ether 5.25%, chloroform 6.34%, ethanol 7.65%, and aqueous 8.35%, and determination of formulation and evaluation of herbal ointment using Achyranthes aspera L. leave extract.

Analgesic activity of Hydroalcholic extract of Achyranthes aspera leaves on animal model

Achyranthes aspera L. (Family: Amaranthaceae) is widely used as a medicinal plant. The hydroalcholic extract of Achyranthes aspera L. leaves was screened for its analgesic activity. The dose (200 mg/kg) was tested for analgesic activity using hot plate and Tail flick test in albino mice. The hydroalcholic extract of Achyranthes aspera L. leaves showed maximum analgesic activity in hot plate at reaction time 120 min (7.40±0.08) and tail flick method at reaction time 120 min (6.9±0.06). These study suggest that the hydroalcholic extract of Achyranthes aspera L. could be considered as potential analgesic agent.

Effect of Achyranthes Aspera Linn. Leaves Extract on Reactive Oxygen Species (ROS) in Diabetes-induced Rats by Flow cytometry and Possible Molecular Mechanism through Molecular Docking

Background::Oxidative stress is caused due to the overproduction of the reactive oxygen species (ROS) and the disturbance developed in the antioxidant potential of biochemical processes. ROS mostly form in the brain due to the high consumption of oxygen and the insufficiency of endogenous antioxidant resistance mechanisms. Cytochrome P450 2E1 has an excessive percentage of NADPH oxidase activity, which causes the production of ROS and increases oxidative stress.Objectives::We have studied the effect of ethyl acetate extract of Achyranthes Aspera (EAAA) on ROS in the brain of diabetes-induced rats. We have also investigated the possible molecular mechanism of reduction in ROS through molecular docking.Methods:To study the oxidative stress induced by ROS in diabetic rats, we estimated the ROS in rat brain through flow cytometry. The oral dose of EAAA 50mg/kg and 100 mg/kg was given to diabetesinduced rats. Results were articulated as mean ± standard deviation (SD). Data were analyzed using analysis of variance (ANOVA) followed by Bonferroni as a post hoc test. We performed molecular docking of flavonoids on CYP2E1 to study the inhibitory potential.Results::The results have shown that EAAA reduces the generation of ROS in the diabetes-induced rat in a dose-dependent manner. The oral dose of EAAA 50mg/kg and 100 mg/kg was given to the rats and the ROS generation got affected accordingly. Luteolin, quercetin, and apigenin inhibited the CYP2E1 very effectively. Luteolin formed 4 hydrogen bonds with CYP2E1, which indicated its potential inhibition. Although, luteolin and apigenin showed a very good binding affinity with the enzyme.Conclusion::From the present work, we have concluded that the ethyl acetate extract of achyrantesaspera can effectively inhibit the ROS generation in the diabetes-induced rats by inhibiting the activity of CYP2E1.

Studies on antibacterial potential and phytochemical screening of different extract of Achyranthes aspera

There is an alarming increase in the problem of resistance towards antibiotics amongst most of the pathogenic bacterial strains in recent years. This has drawn the attention of researchers around the world to search for novel and eco-friendly antibacterial compounds. Several biological sources have been explored in this respect but medicinal plants have taken a centre stage out of all. Plants have been known as a reservoir of number of bioactive compounds specially the antibacterial ones since time immemorial. Therefore, the present investigation was undertaken to analyze the antibacterial potential of the medicinal plant Achyranthes aspera. This study revealed that highest antibacterial activity was observed in the methanolic extract of stem against almost all test Bacteria. It showed maximum activity against E.coli (30 mm), followed by S. aureus (28 mm), Enterococcus sp.(25mm), Salmonella typhi ( 20 mm) and least activity was recorded in same extract against K.pneumoniae (6 mm). Four phytochemicals were screened in various solvent extracts. They are alkaloid, flavonoids, saponins and tannins.

Identification and characterization of phenolic compounds in hydro methanolic extract of Achyranthes aspera (HMEA) by UPLC and MALDI-TOF-MS and in vivo antioxidant activity

Achyranthes aspera is an omnipresent herb of medicinal herbal group, used traditionally as medicine to cure different types of diseases. The components of hydro-methanolic extract of Achyranthes aspera (HMEA), are screened and tentatively identified using UPLC-PDA and MALDI-TOF-MS techniques. The phenolic compounds tentatively identified are quinic acid (m/z 192.040), chlorogenic acid (m/z 354.0894), kaempferol (m/z 286.0917), quercetin (m/z 302.0895) and chrysin (m/z 254.0654) present in the various ratios in which concentration level of chlorogenic acid and kaempferol are determined considering their peak intensity. To the best of our knowledge this is the first report on the characterization of HMEA using UPLC and MALDI-TOF-MS techniques to demonstrate the presence of various phenolic compounds and their antioxidant enzymes activity in vivo. The enzymes which showed increased activity are GST, GR, CAT, and SOD while activity of LDH decreased. These phenolic compounds have potential to be used as anti-cancerous molecules.

Effect of Achyranthes aspera Extract on Phagocytosis by Human Neutrophils

Obejective: To study the effect of hydroalcoholic extract of aerial parts of Achyranthes aspera on neutrophil phagocytic function . Methods: The different concentrations (25,50 and 100 mg / ml ) of extract of aerial parts of Achyranthes aspera was subjected to study its effect on different in vitro methods of phagocytosis such as neutrophil locomotion and chemotaxis, in vitro immunostumulant activity by phyagocytosis of killed Candida albicans and qualitative nitro blue tetrazolium test using human neutrophils. Results: This preliminary study revealed that Achyranthes aspera extract has stimulated chemotactic, phagocytic and intracellular killing potency of human neutrophils at the concentration range of 25 - 100 mg / ml. Conclusion: From the results obtained it can be observed that the hydroalcoholic extract of Achyranthes aspera stimulates cell-mediated immune system by increasing neutrophil phagocytic function.

Enhanced anti-proteases inLabeo rohita fed with diet containing herbal ingredients

Aqueous root extract ofAchyranthes aspera was incorporated in the experimental diet ofLabeo rohita (rohu). Control diet was prepared without root extract. Feeding of fishes with experimental diet has significantly (p<0.05) enhanced the serum anti-proteases level than fishes fed with control diet.